Distribution of Rho-Kinase in the Bovine Brain
Rho-associated kinase (Rho-kinase) is a serine/threonine protein kinase downstream of the small GTPase Rho, which participates in signaling pathways of many cellular functions. Although Rho-kinase is implicated in the regulation of the morphology of neuronal cells, the distribution of Rho-kinase in...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1999-09, Vol.263 (2), p.575-579 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Hashimoto, Ryota Nakamura, Yu Kosako, Hidetaka Amano, Mutsuki Kaibuchi, Kozo Inagaki, Masaki Takeda, Masatoshi |
| description | Rho-associated kinase (Rho-kinase) is a serine/threonine protein kinase downstream of the small GTPase Rho, which participates in signaling pathways of many cellular functions. Although Rho-kinase is implicated in the regulation of the morphology of neuronal cells, the distribution of Rho-kinase in the brain has not been elucidated yet. In this study, we investigated the distribution of Rho-kinase using three antibodies recognizing the different epitopes of Rho-kinase. Rho-kinase was abundantly expressed in the gray matter in comparison with the white matter. Strong immunoreactivity was observed in the pyramidal neurons of the cerebral cortex and hippocampus and in the Purkinje cells of the cerebellum. These results indicate that Rho-kinase is abundantly distributed in neurons and might play an important role in remodeling of neurites. |
| doi_str_mv | 10.1006/bbrc.1999.1409 |
| format | Article |
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Although Rho-kinase is implicated in the regulation of the morphology of neuronal cells, the distribution of Rho-kinase in the brain has not been elucidated yet. In this study, we investigated the distribution of Rho-kinase using three antibodies recognizing the different epitopes of Rho-kinase. Rho-kinase was abundantly expressed in the gray matter in comparison with the white matter. Strong immunoreactivity was observed in the pyramidal neurons of the cerebral cortex and hippocampus and in the Purkinje cells of the cerebellum. These results indicate that Rho-kinase is abundantly distributed in neurons and might play an important role in remodeling of neurites.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1999.1409</identifier><identifier>PMID: 10491334</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Antibody Specificity ; Brain - cytology ; Brain - enzymology ; Cattle ; cerebellum ; Cerebellum - cytology ; cerebral cortex ; Epitopes - immunology ; hippocampus ; Hippocampus - cytology ; immunohistochemistry ; Intracellular Signaling Peptides and Proteins ; neurite retraction ; Protein-Serine-Threonine Kinases - immunology ; Protein-Serine-Threonine Kinases - isolation & purification ; Purkinje Cells ; Pyramidal Cells ; Rho ; rho-Associated Kinases ; Rho-kinase ; Tissue Distribution</subject><ispartof>Biochemical and biophysical research communications, 1999-09, Vol.263 (2), p.575-579</ispartof><rights>1999 Academic Press</rights><rights>Copyright 1999 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-ef4f3b5956acf28797a2f3b322187f9458eebfa1cac9cccfb8b0ab132ff8cc553</citedby><cites>FETCH-LOGICAL-c406t-ef4f3b5956acf28797a2f3b322187f9458eebfa1cac9cccfb8b0ab132ff8cc553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X99914092$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10491334$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hashimoto, Ryota</creatorcontrib><creatorcontrib>Nakamura, Yu</creatorcontrib><creatorcontrib>Kosako, Hidetaka</creatorcontrib><creatorcontrib>Amano, Mutsuki</creatorcontrib><creatorcontrib>Kaibuchi, Kozo</creatorcontrib><creatorcontrib>Inagaki, Masaki</creatorcontrib><creatorcontrib>Takeda, Masatoshi</creatorcontrib><title>Distribution of Rho-Kinase in the Bovine Brain</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Rho-associated kinase (Rho-kinase) is a serine/threonine protein kinase downstream of the small GTPase Rho, which participates in signaling pathways of many cellular functions. Although Rho-kinase is implicated in the regulation of the morphology of neuronal cells, the distribution of Rho-kinase in the brain has not been elucidated yet. In this study, we investigated the distribution of Rho-kinase using three antibodies recognizing the different epitopes of Rho-kinase. Rho-kinase was abundantly expressed in the gray matter in comparison with the white matter. Strong immunoreactivity was observed in the pyramidal neurons of the cerebral cortex and hippocampus and in the Purkinje cells of the cerebellum. These results indicate that Rho-kinase is abundantly distributed in neurons and might play an important role in remodeling of neurites.</description><subject>Animals</subject><subject>Antibody Specificity</subject><subject>Brain - cytology</subject><subject>Brain - enzymology</subject><subject>Cattle</subject><subject>cerebellum</subject><subject>Cerebellum - cytology</subject><subject>cerebral cortex</subject><subject>Epitopes - immunology</subject><subject>hippocampus</subject><subject>Hippocampus - cytology</subject><subject>immunohistochemistry</subject><subject>Intracellular Signaling Peptides and Proteins</subject><subject>neurite retraction</subject><subject>Protein-Serine-Threonine Kinases - immunology</subject><subject>Protein-Serine-Threonine Kinases - isolation & purification</subject><subject>Purkinje Cells</subject><subject>Pyramidal Cells</subject><subject>Rho</subject><subject>rho-Associated Kinases</subject><subject>Rho-kinase</subject><subject>Tissue Distribution</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1LAzEQhoMotlavHmVP3nbN7HeO2vqFBUEUvIVkOqGRdlOT3YL_3l22By-eXph55oV5GLsEngDn5Y3WHhMQQiSQc3HEpsAFj1Pg-TGb8p6IUwGfE3YWwhfnAHkpTtmkXwvIsnzKkoUNrbe6a61rImeit7WLX2yjAkW2ido1RXdub5s-vLLNOTsxahPo4pAz9vFw_z5_ipevj8_z22WMOS_bmExuMl2IolRo0roSlUr7QZamUFdG5EVNpI0CVCgQ0ehac6UhS42pEYsim7HrsXfn3XdHoZVbG5A2G9WQ64KsOC-gygYwGUH0LgRPRu683Sr_I4HLwZAcDMnBkBwM9QdXh-ZOb2n1Bx-V9EA9AtT_t7fkZUBLDdLKesJWrpz9r_sX01x0fg</recordid><startdate>19990924</startdate><enddate>19990924</enddate><creator>Hashimoto, Ryota</creator><creator>Nakamura, Yu</creator><creator>Kosako, Hidetaka</creator><creator>Amano, Mutsuki</creator><creator>Kaibuchi, Kozo</creator><creator>Inagaki, Masaki</creator><creator>Takeda, Masatoshi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990924</creationdate><title>Distribution of Rho-Kinase in the Bovine Brain</title><author>Hashimoto, Ryota ; Nakamura, Yu ; Kosako, Hidetaka ; Amano, Mutsuki ; Kaibuchi, Kozo ; Inagaki, Masaki ; Takeda, Masatoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-ef4f3b5956acf28797a2f3b322187f9458eebfa1cac9cccfb8b0ab132ff8cc553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Antibody Specificity</topic><topic>Brain - cytology</topic><topic>Brain - enzymology</topic><topic>Cattle</topic><topic>cerebellum</topic><topic>Cerebellum - cytology</topic><topic>cerebral cortex</topic><topic>Epitopes - immunology</topic><topic>hippocampus</topic><topic>Hippocampus - cytology</topic><topic>immunohistochemistry</topic><topic>Intracellular Signaling Peptides and Proteins</topic><topic>neurite retraction</topic><topic>Protein-Serine-Threonine Kinases - immunology</topic><topic>Protein-Serine-Threonine Kinases - isolation & purification</topic><topic>Purkinje Cells</topic><topic>Pyramidal Cells</topic><topic>Rho</topic><topic>rho-Associated Kinases</topic><topic>Rho-kinase</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hashimoto, Ryota</creatorcontrib><creatorcontrib>Nakamura, Yu</creatorcontrib><creatorcontrib>Kosako, Hidetaka</creatorcontrib><creatorcontrib>Amano, Mutsuki</creatorcontrib><creatorcontrib>Kaibuchi, Kozo</creatorcontrib><creatorcontrib>Inagaki, Masaki</creatorcontrib><creatorcontrib>Takeda, Masatoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hashimoto, Ryota</au><au>Nakamura, Yu</au><au>Kosako, Hidetaka</au><au>Amano, Mutsuki</au><au>Kaibuchi, Kozo</au><au>Inagaki, Masaki</au><au>Takeda, Masatoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of Rho-Kinase in the Bovine Brain</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1999-09-24</date><risdate>1999</risdate><volume>263</volume><issue>2</issue><spage>575</spage><epage>579</epage><pages>575-579</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Rho-associated kinase (Rho-kinase) is a serine/threonine protein kinase downstream of the small GTPase Rho, which participates in signaling pathways of many cellular functions. Although Rho-kinase is implicated in the regulation of the morphology of neuronal cells, the distribution of Rho-kinase in the brain has not been elucidated yet. In this study, we investigated the distribution of Rho-kinase using three antibodies recognizing the different epitopes of Rho-kinase. Rho-kinase was abundantly expressed in the gray matter in comparison with the white matter. Strong immunoreactivity was observed in the pyramidal neurons of the cerebral cortex and hippocampus and in the Purkinje cells of the cerebellum. These results indicate that Rho-kinase is abundantly distributed in neurons and might play an important role in remodeling of neurites.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10491334</pmid><doi>10.1006/bbrc.1999.1409</doi><tpages>5</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1999-09, Vol.263 (2), p.575-579 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibody Specificity Brain - cytology Brain - enzymology Cattle cerebellum Cerebellum - cytology cerebral cortex Epitopes - immunology hippocampus Hippocampus - cytology immunohistochemistry Intracellular Signaling Peptides and Proteins neurite retraction Protein-Serine-Threonine Kinases - immunology Protein-Serine-Threonine Kinases - isolation & purification Purkinje Cells Pyramidal Cells Rho rho-Associated Kinases Rho-kinase Tissue Distribution |
| title | Distribution of Rho-Kinase in the Bovine Brain |
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