A one-step solid phase immunoassay for simultaneous detection of serum IgG and IgM antibodies to Borrelia burgdorferi
A one-step immunoassay for simultaneous detection of serum IgG and IgM antibodies to Borrelia burgdorferi has been developed. The assay is based on C1q, which binds to immune complexes containing IgG and/or IgM antibodies. Micro-beads pre-coated with antibodies to human C1q are mixed with human seru...
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| Veröffentlicht in: | Journal of immunological methods 1998-09, Vol.218 (1), p.9-17 |
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| creator | Schønau, Andreas Stender, Henrik Grauballe, Per Chr |
| description | A one-step immunoassay for simultaneous detection of serum IgG and IgM antibodies to
Borrelia burgdorferi has been developed. The assay is based on C1q, which binds to immune complexes containing IgG and/or IgM antibodies. Micro-beads pre-coated with antibodies to human C1q are mixed with human serum samples and fluorochrome-labelled
B. burgdorferi flagellum antigen. In the presence of serum IgG and/or IgM antibodies to
B. burgdorferi, fluorochrome-labelled antigen/antibody complexes are formed. These are then bound by serum C1q and are subsequently captured on the anti-C1q-coated beads. The sample is analysed on a flow cytometer and the presence of fluorescent beads is, thus, indicative of a positive test result. In the present study the sensitivity and specificity of the assay are compared to those of the indirect IDEIA™
B. burgdorferi IgG and the
μ-chain capture IDEIA™
B. burgdorferi IgM ELISAs for separate determination of IgG and IgM. Detection using a flow cytometer can be performed without separation of the beads from the reaction mixture, which means that in practice, the method is carried out as a one-step assay and it is, thus, very suitable for automation. Other advantages of this kind of assay includes an antibody/antigen reaction which occurs in solution and the potential of using the method for the detection of antibodies against several antigens from the same or different infectious agents (multi-parameter screening). |
| doi_str_mv | 10.1016/S0022-1759(98)00063-5 |
| format | Article |
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Borrelia burgdorferi has been developed. The assay is based on C1q, which binds to immune complexes containing IgG and/or IgM antibodies. Micro-beads pre-coated with antibodies to human C1q are mixed with human serum samples and fluorochrome-labelled
B. burgdorferi flagellum antigen. In the presence of serum IgG and/or IgM antibodies to
B. burgdorferi, fluorochrome-labelled antigen/antibody complexes are formed. These are then bound by serum C1q and are subsequently captured on the anti-C1q-coated beads. The sample is analysed on a flow cytometer and the presence of fluorescent beads is, thus, indicative of a positive test result. In the present study the sensitivity and specificity of the assay are compared to those of the indirect IDEIA™
B. burgdorferi IgG and the
μ-chain capture IDEIA™
B. burgdorferi IgM ELISAs for separate determination of IgG and IgM. Detection using a flow cytometer can be performed without separation of the beads from the reaction mixture, which means that in practice, the method is carried out as a one-step assay and it is, thus, very suitable for automation. Other advantages of this kind of assay includes an antibody/antigen reaction which occurs in solution and the potential of using the method for the detection of antibodies against several antigens from the same or different infectious agents (multi-parameter screening).</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/S0022-1759(98)00063-5</identifier><identifier>PMID: 9819119</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Antibodies, Bacterial - blood ; Antigen-Antibody Complex ; Antigens, Bacterial - immunology ; Bacterial diseases ; Biological and medical sciences ; Borrelia burgdorferi ; Borrelia infections ; C1q ; Complement C1q ; Enzyme-Linked Immunosorbent Assay - methods ; Flow cytometer ; Flow Cytometry ; Fluorescent Antibody Technique, Indirect ; Human bacterial diseases ; Humans ; Immunoassay - methods ; Immunoglobulin G - blood ; Immunoglobulin M - blood ; Infectious diseases ; Lyme Disease - blood ; Lyme Disease - epidemiology ; Medical sciences ; Micro-beads ; Multi-parameter screening ; One-step immunoassay ; Prevalence ; Prospective Studies ; Sensitivity and Specificity ; Tropical bacterial diseases</subject><ispartof>Journal of immunological methods, 1998-09, Vol.218 (1), p.9-17</ispartof><rights>1998 Elsevier Science B.V.</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-883e7d8c60f734ae599b904fe65faee6e84d0629f26a109c1c4bdc35d0dd0f7a3</citedby><cites>FETCH-LOGICAL-c420t-883e7d8c60f734ae599b904fe65faee6e84d0629f26a109c1c4bdc35d0dd0f7a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0022-1759(98)00063-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1584186$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9819119$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schønau, Andreas</creatorcontrib><creatorcontrib>Stender, Henrik</creatorcontrib><creatorcontrib>Grauballe, Per Chr</creatorcontrib><title>A one-step solid phase immunoassay for simultaneous detection of serum IgG and IgM antibodies to Borrelia burgdorferi</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>A one-step immunoassay for simultaneous detection of serum IgG and IgM antibodies to
Borrelia burgdorferi has been developed. The assay is based on C1q, which binds to immune complexes containing IgG and/or IgM antibodies. Micro-beads pre-coated with antibodies to human C1q are mixed with human serum samples and fluorochrome-labelled
B. burgdorferi flagellum antigen. In the presence of serum IgG and/or IgM antibodies to
B. burgdorferi, fluorochrome-labelled antigen/antibody complexes are formed. These are then bound by serum C1q and are subsequently captured on the anti-C1q-coated beads. The sample is analysed on a flow cytometer and the presence of fluorescent beads is, thus, indicative of a positive test result. In the present study the sensitivity and specificity of the assay are compared to those of the indirect IDEIA™
B. burgdorferi IgG and the
μ-chain capture IDEIA™
B. burgdorferi IgM ELISAs for separate determination of IgG and IgM. Detection using a flow cytometer can be performed without separation of the beads from the reaction mixture, which means that in practice, the method is carried out as a one-step assay and it is, thus, very suitable for automation. Other advantages of this kind of assay includes an antibody/antigen reaction which occurs in solution and the potential of using the method for the detection of antibodies against several antigens from the same or different infectious agents (multi-parameter screening).</description><subject>Antibodies, Bacterial - blood</subject><subject>Antigen-Antibody Complex</subject><subject>Antigens, Bacterial - immunology</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>Borrelia burgdorferi</subject><subject>Borrelia infections</subject><subject>C1q</subject><subject>Complement C1q</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Flow cytometer</subject><subject>Flow Cytometry</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Immunoassay - methods</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin M - blood</subject><subject>Infectious diseases</subject><subject>Lyme Disease - blood</subject><subject>Lyme Disease - epidemiology</subject><subject>Medical sciences</subject><subject>Micro-beads</subject><subject>Multi-parameter screening</subject><subject>One-step immunoassay</subject><subject>Prevalence</subject><subject>Prospective Studies</subject><subject>Sensitivity and Specificity</subject><subject>Tropical bacterial diseases</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EKtvCR6jkA0JwCIyzsWOfUFvRUqmIA3C2HHtcjJJ48SRI_fa43VU59vQO83vz7zF2KuCDAKE-fgdo20b00rwz-j0AqG0jn7GN0H3b9Abkc7Z5RF6yY6LfFRKg4IgdGS2MEGbD1jOeZ2xowR2nPKbAd78cIU_TtM7ZEbk7HnPhlKZ1XNyMeSUecEG_pDzzHDlhWSd-fXvF3Ryqfq26pCGHhMSXzM9zKTgmx4e13IZcIpb0ir2IbiR8fdAT9vPy84-LL83Nt6vri7ObxnctLI3WW-yD9gpiv-0cSmMGA11EJaNDVKi7AKo1sVVOgPHCd0PwWxkghGpx2xP2dt93V_KfFWmxUyKP47g_xPYAUnTQPgkKJVvoW1NBuQd9yUQFo92VNLlyZwXY-1zsQy72_unWaPuQi5XVd3oYsA4ThkfXIYhaf3OoO_JujMXNPtH_5lJ3QquKfdpjWL_2N2Gx5BPOHkMqNRIbcnpikX-2_qr7</recordid><startdate>19980901</startdate><enddate>19980901</enddate><creator>Schønau, Andreas</creator><creator>Stender, Henrik</creator><creator>Grauballe, Per Chr</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19980901</creationdate><title>A one-step solid phase immunoassay for simultaneous detection of serum IgG and IgM antibodies to Borrelia burgdorferi</title><author>Schønau, Andreas ; Stender, Henrik ; Grauballe, Per Chr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-883e7d8c60f734ae599b904fe65faee6e84d0629f26a109c1c4bdc35d0dd0f7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Antibodies, Bacterial - blood</topic><topic>Antigen-Antibody Complex</topic><topic>Antigens, Bacterial - immunology</topic><topic>Bacterial diseases</topic><topic>Biological and medical sciences</topic><topic>Borrelia burgdorferi</topic><topic>Borrelia infections</topic><topic>C1q</topic><topic>Complement C1q</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Flow cytometer</topic><topic>Flow Cytometry</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Immunoassay - methods</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulin M - blood</topic><topic>Infectious diseases</topic><topic>Lyme Disease - blood</topic><topic>Lyme Disease - epidemiology</topic><topic>Medical sciences</topic><topic>Micro-beads</topic><topic>Multi-parameter screening</topic><topic>One-step immunoassay</topic><topic>Prevalence</topic><topic>Prospective Studies</topic><topic>Sensitivity and Specificity</topic><topic>Tropical bacterial diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schønau, Andreas</creatorcontrib><creatorcontrib>Stender, Henrik</creatorcontrib><creatorcontrib>Grauballe, Per Chr</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schønau, Andreas</au><au>Stender, Henrik</au><au>Grauballe, Per Chr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A one-step solid phase immunoassay for simultaneous detection of serum IgG and IgM antibodies to Borrelia burgdorferi</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1998-09-01</date><risdate>1998</risdate><volume>218</volume><issue>1</issue><spage>9</spage><epage>17</epage><pages>9-17</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>A one-step immunoassay for simultaneous detection of serum IgG and IgM antibodies to
Borrelia burgdorferi has been developed. The assay is based on C1q, which binds to immune complexes containing IgG and/or IgM antibodies. Micro-beads pre-coated with antibodies to human C1q are mixed with human serum samples and fluorochrome-labelled
B. burgdorferi flagellum antigen. In the presence of serum IgG and/or IgM antibodies to
B. burgdorferi, fluorochrome-labelled antigen/antibody complexes are formed. These are then bound by serum C1q and are subsequently captured on the anti-C1q-coated beads. The sample is analysed on a flow cytometer and the presence of fluorescent beads is, thus, indicative of a positive test result. In the present study the sensitivity and specificity of the assay are compared to those of the indirect IDEIA™
B. burgdorferi IgG and the
μ-chain capture IDEIA™
B. burgdorferi IgM ELISAs for separate determination of IgG and IgM. Detection using a flow cytometer can be performed without separation of the beads from the reaction mixture, which means that in practice, the method is carried out as a one-step assay and it is, thus, very suitable for automation. Other advantages of this kind of assay includes an antibody/antigen reaction which occurs in solution and the potential of using the method for the detection of antibodies against several antigens from the same or different infectious agents (multi-parameter screening).</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>9819119</pmid><doi>10.1016/S0022-1759(98)00063-5</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of immunological methods, 1998-09, Vol.218 (1), p.9-17 |
| issn | 0022-1759 1872-7905 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Antibodies, Bacterial - blood Antigen-Antibody Complex Antigens, Bacterial - immunology Bacterial diseases Biological and medical sciences Borrelia burgdorferi Borrelia infections C1q Complement C1q Enzyme-Linked Immunosorbent Assay - methods Flow cytometer Flow Cytometry Fluorescent Antibody Technique, Indirect Human bacterial diseases Humans Immunoassay - methods Immunoglobulin G - blood Immunoglobulin M - blood Infectious diseases Lyme Disease - blood Lyme Disease - epidemiology Medical sciences Micro-beads Multi-parameter screening One-step immunoassay Prevalence Prospective Studies Sensitivity and Specificity Tropical bacterial diseases |
| title | A one-step solid phase immunoassay for simultaneous detection of serum IgG and IgM antibodies to Borrelia burgdorferi |
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