Peripheral blood cytokine responses and disease severity in respiratory syncytial virus bronchiolitis
The role of cellular immunity in disease severity in respiratory syncytial virus (RSV) bronchiolitis is largely unknown. This study investigated the association between disease severity and systemic cytokine responses in hospitalized ventilated and nonventilated RSV bronchiolitis patients. In whole...
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| Veröffentlicht in: | The European respiratory journal 1999-07, Vol.14 (1), p.144-149 |
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| creator | Bont, L Heijnen, CJ Kavelaars, A van Aalderen, WM Brus, F Draaisma, JT Geelen, SM van Vught, HJ Kimpen, JL |
| description | The role of cellular immunity in disease severity in respiratory syncytial virus (RSV) bronchiolitis is largely unknown. This study investigated the association between disease severity and systemic cytokine responses in hospitalized ventilated and nonventilated RSV bronchiolitis patients.
In whole blood cultures stimulated with phytohaemagglutinin (PHA), lymphoproliferative responses and interferon (IFN)‐γ and interleukin (IL)‐4 production during acute illness were measured. In addition, plasma cytokines were measured. Measurements were repeated in the convalescent phase, 3–4 weeks after admission.
Fifty patients were included. The median age in ventilaled patients was significantly lower than in nonventilated patients (1 versus 4 months, p |
| doi_str_mv | 10.1034/j.1399-3003.1999.14a24.x |
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In whole blood cultures stimulated with phytohaemagglutinin (PHA), lymphoproliferative responses and interferon (IFN)‐γ and interleukin (IL)‐4 production during acute illness were measured. In addition, plasma cytokines were measured. Measurements were repeated in the convalescent phase, 3–4 weeks after admission.
Fifty patients were included. The median age in ventilaled patients was significantly lower than in nonventilated patients (1 versus 4 months, p<0.05). In comparison with nonventilated patients, the ventilated patients had significantly lower lymphoproliferative responses and a lower production of IFN‐γ and IL‐4. In fact, IFN‐γ and IL‐4 production in ventilated patients was almost completely undetectable. Plasma IL‐8 levels in ventilated patients were significantly higher than in nonventilated patients. In the convalescent phase, lymphoproliferative and cytokine responses as well as plasma IL‐8 levels were normal in both patient groups. Since RSV bronchiolitis is associated with the subsequent development of asthma, the possible skewing of the T‐helper (Th1/Th2) cytokine balance was investigated. This was found neither in the acute nor in the convalescent phase.
In conclusion, the data indicate that depressed lymphocyte function and elevated plasma interleukin‐8 levels are markers of severe disease. It is suggested that age and maturation related immune mechanisms could explain the occurrence of severe respiratory syncytial virus bronchiolitis requiring mechanical ventilation in young infants.</description><identifier>ISSN: 0903-1936</identifier><identifier>EISSN: 1399-3003</identifier><identifier>DOI: 10.1034/j.1399-3003.1999.14a24.x</identifier><identifier>PMID: 10489842</identifier><language>eng</language><publisher>Oxford, UK: Eur Respiratory Soc</publisher><subject>AIDS/HIV ; Antibodies, Viral - analysis ; Biological and medical sciences ; Biomarkers - blood ; Bronchiolitis, Viral - blood ; Bronchiolitis, Viral - immunology ; Bronchiolitis, Viral - virology ; Cells, Cultured ; Cytokines ; disease severity ; Female ; Human viral diseases ; Humans ; Infant ; Infant, Newborn ; Infectious diseases ; Interferon-gamma - blood ; Interleukin-12 - blood ; Interleukin-4 - blood ; Interleukin-8 - blood ; Male ; Medical sciences ; Phytohemagglutinins ; respiratory syncytial virus ; Respiratory Syncytial Virus Infections - blood ; Respiratory Syncytial Virus Infections - immunology ; Respiratory Syncytial Virus Infections - virology ; Respiratory Syncytial Viruses - immunology ; Respiratory Syncytial Viruses - isolation & purification ; Severity of Illness Index ; Th1 Cells - drug effects ; Th1 Cells - metabolism ; Th1 Cells - pathology ; Th2 Cells - drug effects ; Th2 Cells - metabolism ; Th2 Cells - pathology ; Viral diseases ; Viral diseases of the respiratory system and ent viral diseases</subject><ispartof>The European respiratory journal, 1999-07, Vol.14 (1), p.144-149</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4754-19853ce2f41ce961fd2cd6da9e58d404ae5ee0d1145ecfb2a2579b6cc111b7033</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1034%2Fj.1399-3003.1999.14a24.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27933,27934,45584</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1929723$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10489842$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bont, L</creatorcontrib><creatorcontrib>Heijnen, CJ</creatorcontrib><creatorcontrib>Kavelaars, A</creatorcontrib><creatorcontrib>van Aalderen, WM</creatorcontrib><creatorcontrib>Brus, F</creatorcontrib><creatorcontrib>Draaisma, JT</creatorcontrib><creatorcontrib>Geelen, SM</creatorcontrib><creatorcontrib>van Vught, HJ</creatorcontrib><creatorcontrib>Kimpen, JL</creatorcontrib><title>Peripheral blood cytokine responses and disease severity in respiratory syncytial virus bronchiolitis</title><title>The European respiratory journal</title><addtitle>Eur Respir J</addtitle><description>The role of cellular immunity in disease severity in respiratory syncytial virus (RSV) bronchiolitis is largely unknown. This study investigated the association between disease severity and systemic cytokine responses in hospitalized ventilated and nonventilated RSV bronchiolitis patients.
In whole blood cultures stimulated with phytohaemagglutinin (PHA), lymphoproliferative responses and interferon (IFN)‐γ and interleukin (IL)‐4 production during acute illness were measured. In addition, plasma cytokines were measured. Measurements were repeated in the convalescent phase, 3–4 weeks after admission.
Fifty patients were included. The median age in ventilaled patients was significantly lower than in nonventilated patients (1 versus 4 months, p<0.05). In comparison with nonventilated patients, the ventilated patients had significantly lower lymphoproliferative responses and a lower production of IFN‐γ and IL‐4. In fact, IFN‐γ and IL‐4 production in ventilated patients was almost completely undetectable. Plasma IL‐8 levels in ventilated patients were significantly higher than in nonventilated patients. In the convalescent phase, lymphoproliferative and cytokine responses as well as plasma IL‐8 levels were normal in both patient groups. Since RSV bronchiolitis is associated with the subsequent development of asthma, the possible skewing of the T‐helper (Th1/Th2) cytokine balance was investigated. This was found neither in the acute nor in the convalescent phase.
In conclusion, the data indicate that depressed lymphocyte function and elevated plasma interleukin‐8 levels are markers of severe disease. It is suggested that age and maturation related immune mechanisms could explain the occurrence of severe respiratory syncytial virus bronchiolitis requiring mechanical ventilation in young infants.</description><subject>AIDS/HIV</subject><subject>Antibodies, Viral - analysis</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - blood</subject><subject>Bronchiolitis, Viral - blood</subject><subject>Bronchiolitis, Viral - immunology</subject><subject>Bronchiolitis, Viral - virology</subject><subject>Cells, Cultured</subject><subject>Cytokines</subject><subject>disease severity</subject><subject>Female</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Infectious diseases</subject><subject>Interferon-gamma - blood</subject><subject>Interleukin-12 - blood</subject><subject>Interleukin-4 - blood</subject><subject>Interleukin-8 - blood</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Phytohemagglutinins</subject><subject>respiratory syncytial virus</subject><subject>Respiratory Syncytial Virus Infections - blood</subject><subject>Respiratory Syncytial Virus Infections - immunology</subject><subject>Respiratory Syncytial Virus Infections - virology</subject><subject>Respiratory Syncytial Viruses - immunology</subject><subject>Respiratory Syncytial Viruses - isolation & purification</subject><subject>Severity of Illness Index</subject><subject>Th1 Cells - drug effects</subject><subject>Th1 Cells - metabolism</subject><subject>Th1 Cells - pathology</subject><subject>Th2 Cells - drug effects</subject><subject>Th2 Cells - metabolism</subject><subject>Th2 Cells - pathology</subject><subject>Viral diseases</subject><subject>Viral diseases of the respiratory system and ent viral diseases</subject><issn>0903-1936</issn><issn>1399-3003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtv1DAUhS0EokPhLyAvoLsJvrHz8AapqspLlUAI1pbj3DAePPHgO9M2_x6nGQFLVl74O-cefYxxEAUIqd5sC5Bar6UQsgCtdQHKlqq4f8RWfz4es5XQQq5By_qMPSPaCgG1kvCUnYFQrW5VuWL4BZPfbzDZwLsQY8_ddIg__Yg8Ie3jSEjcjj3vPaEl5IS3OXGYuB8fCJ_sIaaJ0zTmpM81tz4diXcpjm7jY_AHT8_Zk8EGwhen95x9f3f97erD-ubz-49Xlzdrp5pK5altJR2WgwKHuoahL11f91Zj1fZKKIsVougBVIVu6EpbVo3uaucAoGuElOfsYundp_jriHQwO08OQ7AjxiOZRghV1XWTwXYBXYpECQezT35n02RAmFmx2ZrZpJlNmlmxeVBs7nP05enGsdth_09wcZqBVyfAkrNhSHZ0nv5yutRNOW99u2B3PuD03_fN9ddPoC5LlQteLwUb_2Nz5xMa2tkQ8iwwmLagDOSQkr8Bx4Snog</recordid><startdate>199907</startdate><enddate>199907</enddate><creator>Bont, L</creator><creator>Heijnen, CJ</creator><creator>Kavelaars, A</creator><creator>van Aalderen, WM</creator><creator>Brus, F</creator><creator>Draaisma, JT</creator><creator>Geelen, SM</creator><creator>van Vught, HJ</creator><creator>Kimpen, JL</creator><general>Eur Respiratory Soc</general><general>European Respiratory Journal</general><general>Maney</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199907</creationdate><title>Peripheral blood cytokine responses and disease severity in respiratory syncytial virus bronchiolitis</title><author>Bont, L ; Heijnen, CJ ; Kavelaars, A ; van Aalderen, WM ; Brus, F ; Draaisma, JT ; Geelen, SM ; van Vught, HJ ; Kimpen, JL</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4754-19853ce2f41ce961fd2cd6da9e58d404ae5ee0d1145ecfb2a2579b6cc111b7033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>AIDS/HIV</topic><topic>Antibodies, Viral - analysis</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - blood</topic><topic>Bronchiolitis, Viral - blood</topic><topic>Bronchiolitis, Viral - immunology</topic><topic>Bronchiolitis, Viral - virology</topic><topic>Cells, Cultured</topic><topic>Cytokines</topic><topic>disease severity</topic><topic>Female</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Infectious diseases</topic><topic>Interferon-gamma - blood</topic><topic>Interleukin-12 - blood</topic><topic>Interleukin-4 - blood</topic><topic>Interleukin-8 - blood</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Phytohemagglutinins</topic><topic>respiratory syncytial virus</topic><topic>Respiratory Syncytial Virus Infections - blood</topic><topic>Respiratory Syncytial Virus Infections - immunology</topic><topic>Respiratory Syncytial Virus Infections - virology</topic><topic>Respiratory Syncytial Viruses - immunology</topic><topic>Respiratory Syncytial Viruses - isolation & purification</topic><topic>Severity of Illness Index</topic><topic>Th1 Cells - drug effects</topic><topic>Th1 Cells - metabolism</topic><topic>Th1 Cells - pathology</topic><topic>Th2 Cells - drug effects</topic><topic>Th2 Cells - metabolism</topic><topic>Th2 Cells - pathology</topic><topic>Viral diseases</topic><topic>Viral diseases of the respiratory system and ent viral diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bont, L</creatorcontrib><creatorcontrib>Heijnen, CJ</creatorcontrib><creatorcontrib>Kavelaars, A</creatorcontrib><creatorcontrib>van Aalderen, WM</creatorcontrib><creatorcontrib>Brus, F</creatorcontrib><creatorcontrib>Draaisma, JT</creatorcontrib><creatorcontrib>Geelen, SM</creatorcontrib><creatorcontrib>van Vught, HJ</creatorcontrib><creatorcontrib>Kimpen, JL</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The European respiratory journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bont, L</au><au>Heijnen, CJ</au><au>Kavelaars, A</au><au>van Aalderen, WM</au><au>Brus, F</au><au>Draaisma, JT</au><au>Geelen, SM</au><au>van Vught, HJ</au><au>Kimpen, JL</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Peripheral blood cytokine responses and disease severity in respiratory syncytial virus bronchiolitis</atitle><jtitle>The European respiratory journal</jtitle><addtitle>Eur Respir J</addtitle><date>1999-07</date><risdate>1999</risdate><volume>14</volume><issue>1</issue><spage>144</spage><epage>149</epage><pages>144-149</pages><issn>0903-1936</issn><eissn>1399-3003</eissn><abstract>The role of cellular immunity in disease severity in respiratory syncytial virus (RSV) bronchiolitis is largely unknown. This study investigated the association between disease severity and systemic cytokine responses in hospitalized ventilated and nonventilated RSV bronchiolitis patients.
In whole blood cultures stimulated with phytohaemagglutinin (PHA), lymphoproliferative responses and interferon (IFN)‐γ and interleukin (IL)‐4 production during acute illness were measured. In addition, plasma cytokines were measured. Measurements were repeated in the convalescent phase, 3–4 weeks after admission.
Fifty patients were included. The median age in ventilaled patients was significantly lower than in nonventilated patients (1 versus 4 months, p<0.05). In comparison with nonventilated patients, the ventilated patients had significantly lower lymphoproliferative responses and a lower production of IFN‐γ and IL‐4. In fact, IFN‐γ and IL‐4 production in ventilated patients was almost completely undetectable. Plasma IL‐8 levels in ventilated patients were significantly higher than in nonventilated patients. In the convalescent phase, lymphoproliferative and cytokine responses as well as plasma IL‐8 levels were normal in both patient groups. Since RSV bronchiolitis is associated with the subsequent development of asthma, the possible skewing of the T‐helper (Th1/Th2) cytokine balance was investigated. This was found neither in the acute nor in the convalescent phase.
In conclusion, the data indicate that depressed lymphocyte function and elevated plasma interleukin‐8 levels are markers of severe disease. It is suggested that age and maturation related immune mechanisms could explain the occurrence of severe respiratory syncytial virus bronchiolitis requiring mechanical ventilation in young infants.</abstract><cop>Oxford, UK</cop><pub>Eur Respiratory Soc</pub><pmid>10489842</pmid><doi>10.1034/j.1399-3003.1999.14a24.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The European respiratory journal, 1999-07, Vol.14 (1), p.144-149 |
| issn | 0903-1936 1399-3003 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via Wiley Online Library |
| subjects | AIDS/HIV Antibodies, Viral - analysis Biological and medical sciences Biomarkers - blood Bronchiolitis, Viral - blood Bronchiolitis, Viral - immunology Bronchiolitis, Viral - virology Cells, Cultured Cytokines disease severity Female Human viral diseases Humans Infant Infant, Newborn Infectious diseases Interferon-gamma - blood Interleukin-12 - blood Interleukin-4 - blood Interleukin-8 - blood Male Medical sciences Phytohemagglutinins respiratory syncytial virus Respiratory Syncytial Virus Infections - blood Respiratory Syncytial Virus Infections - immunology Respiratory Syncytial Virus Infections - virology Respiratory Syncytial Viruses - immunology Respiratory Syncytial Viruses - isolation & purification Severity of Illness Index Th1 Cells - drug effects Th1 Cells - metabolism Th1 Cells - pathology Th2 Cells - drug effects Th2 Cells - metabolism Th2 Cells - pathology Viral diseases Viral diseases of the respiratory system and ent viral diseases |
| title | Peripheral blood cytokine responses and disease severity in respiratory syncytial virus bronchiolitis |
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