Smaug, a Novel RNA-Binding Protein that Operates a Translational Switch in Drosophila
During Drosophila embryogenesis, a gradient of Nanos protein emanating from the posterior pole organizes abdominal segmentation. This gradient arises from translational regulation of nanos mRNA, which is activated in the specialized cytoplasm at the posterior pole of the embryo and repressed elsewhe...
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| Veröffentlicht in: | Molecular cell 1999-08, Vol.4 (2), p.209-218 |
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| creator | Dahanukar, Anupama Walker, James A Wharton, Robin P |
| description | During
Drosophila embryogenesis, a gradient of Nanos protein emanating from the posterior pole organizes abdominal segmentation. This gradient arises from translational regulation of
nanos mRNA, which is activated in the specialized cytoplasm at the posterior pole of the embryo and repressed elsewhere. Previously, we have defined
cis-acting elements in the mRNA that mediate this translational switch. In this report, we identify a factor named Smaug that binds to these elements and represses translation in the bulk cytoplasm. Smaug interacts genetically and biochemically with Oskar, a key component of the pole plasm for activation of
nanos mRNA and specification of the germline precursors. These observations suggest that Smaug operates a translational switch that governs the distribution of Nanos protein. |
| doi_str_mv | 10.1016/S1097-2765(00)80368-8 |
| format | Article |
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Drosophila embryogenesis, a gradient of Nanos protein emanating from the posterior pole organizes abdominal segmentation. This gradient arises from translational regulation of
nanos mRNA, which is activated in the specialized cytoplasm at the posterior pole of the embryo and repressed elsewhere. Previously, we have defined
cis-acting elements in the mRNA that mediate this translational switch. In this report, we identify a factor named Smaug that binds to these elements and represses translation in the bulk cytoplasm. Smaug interacts genetically and biochemically with Oskar, a key component of the pole plasm for activation of
nanos mRNA and specification of the germline precursors. These observations suggest that Smaug operates a translational switch that governs the distribution of Nanos protein.</description><identifier>ISSN: 1097-2765</identifier><identifier>EISSN: 1097-4164</identifier><identifier>DOI: 10.1016/S1097-2765(00)80368-8</identifier><identifier>PMID: 10488336</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Abdomen ; Amino Acid Sequence ; Animals ; Animals, Genetically Modified ; Base Sequence ; Binding Sites ; Drosophila ; Drosophila melanogaster - embryology ; Drosophila melanogaster - genetics ; Drosophila Proteins ; Embryo, Nonmammalian - physiology ; Gene Expression Regulation, Developmental ; Genomic Library ; Insect Proteins - genetics ; Molecular Sequence Data ; Morphogenesis ; nanos (nos) gene ; nanos gene ; Oskar protein ; Promoter Regions, Genetic ; Protein Biosynthesis ; Recombinant Fusion Proteins - biosynthesis ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; RNA-Binding Proteins - chemistry ; RNA-Binding Proteins - genetics ; RNA-Binding Proteins - metabolism ; Smaug protein</subject><ispartof>Molecular cell, 1999-08, Vol.4 (2), p.209-218</ispartof><rights>1999 Cell Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-e133c8c24a7a83fedf1b290611e9bd702548be35c98e8e873598f251c3b060ec3</citedby><cites>FETCH-LOGICAL-c439t-e133c8c24a7a83fedf1b290611e9bd702548be35c98e8e873598f251c3b060ec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1097276500803688$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10488336$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dahanukar, Anupama</creatorcontrib><creatorcontrib>Walker, James A</creatorcontrib><creatorcontrib>Wharton, Robin P</creatorcontrib><title>Smaug, a Novel RNA-Binding Protein that Operates a Translational Switch in Drosophila</title><title>Molecular cell</title><addtitle>Mol Cell</addtitle><description>During
Drosophila embryogenesis, a gradient of Nanos protein emanating from the posterior pole organizes abdominal segmentation. This gradient arises from translational regulation of
nanos mRNA, which is activated in the specialized cytoplasm at the posterior pole of the embryo and repressed elsewhere. Previously, we have defined
cis-acting elements in the mRNA that mediate this translational switch. In this report, we identify a factor named Smaug that binds to these elements and represses translation in the bulk cytoplasm. Smaug interacts genetically and biochemically with Oskar, a key component of the pole plasm for activation of
nanos mRNA and specification of the germline precursors. These observations suggest that Smaug operates a translational switch that governs the distribution of Nanos protein.</description><subject>Abdomen</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Drosophila</subject><subject>Drosophila melanogaster - embryology</subject><subject>Drosophila melanogaster - genetics</subject><subject>Drosophila Proteins</subject><subject>Embryo, Nonmammalian - physiology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genomic Library</subject><subject>Insect Proteins - genetics</subject><subject>Molecular Sequence Data</subject><subject>Morphogenesis</subject><subject>nanos (nos) gene</subject><subject>nanos gene</subject><subject>Oskar protein</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Biosynthesis</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA-Binding Proteins - chemistry</subject><subject>RNA-Binding Proteins - genetics</subject><subject>RNA-Binding Proteins - metabolism</subject><subject>Smaug protein</subject><issn>1097-2765</issn><issn>1097-4164</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtPwzAMgCMEYmPwE0A9IZAoOE2bpic03kiIIbadozR1t6CuHUkK4t_TPQ7ckA-2rM-2_BFyTOGSAuVXYwpZGkYpT84AzgUwLkKxQ_rrdkx5vLutV0iPHDj3AUDjRGT7pEchFoIx3ifT8UK1s4tABa_NF1bB--swvDF1YepZ8GYbj6YO_Fz5YLREqzy6jpxYVbtKedPUqgrG38bredBxd7ZxzXJuKnVI9kpVOTza5gGZPtxPbp_Cl9Hj8-3wJdQxy3yIlDEtdBSrVAlWYlHSPMqAU4pZXqQQJbHIkSU6E9hFypJMlFFCNcuBA2o2IKebvUvbfLbovFwYp7GqVI1N62QKECecR_-CNGVC0M7JgCQbUHfPOIulXFqzUPZHUpAr8XItXq6sSgC5Fi9XcyfbA22-wOLP1MZ0B1xvAOx8fBm00mmDtcbCWNReFo3558Qv9CCRdw</recordid><startdate>19990801</startdate><enddate>19990801</enddate><creator>Dahanukar, Anupama</creator><creator>Walker, James A</creator><creator>Wharton, Robin P</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19990801</creationdate><title>Smaug, a Novel RNA-Binding Protein that Operates a Translational Switch in Drosophila</title><author>Dahanukar, Anupama ; Walker, James A ; Wharton, Robin P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-e133c8c24a7a83fedf1b290611e9bd702548be35c98e8e873598f251c3b060ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Abdomen</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Animals, Genetically Modified</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Drosophila</topic><topic>Drosophila melanogaster - embryology</topic><topic>Drosophila melanogaster - genetics</topic><topic>Drosophila Proteins</topic><topic>Embryo, Nonmammalian - physiology</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genomic Library</topic><topic>Insect Proteins - genetics</topic><topic>Molecular Sequence Data</topic><topic>Morphogenesis</topic><topic>nanos (nos) gene</topic><topic>nanos gene</topic><topic>Oskar protein</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Biosynthesis</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA-Binding Proteins - chemistry</topic><topic>RNA-Binding Proteins - genetics</topic><topic>RNA-Binding Proteins - metabolism</topic><topic>Smaug protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dahanukar, Anupama</creatorcontrib><creatorcontrib>Walker, James A</creatorcontrib><creatorcontrib>Wharton, Robin P</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dahanukar, Anupama</au><au>Walker, James A</au><au>Wharton, Robin P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Smaug, a Novel RNA-Binding Protein that Operates a Translational Switch in Drosophila</atitle><jtitle>Molecular cell</jtitle><addtitle>Mol Cell</addtitle><date>1999-08-01</date><risdate>1999</risdate><volume>4</volume><issue>2</issue><spage>209</spage><epage>218</epage><pages>209-218</pages><issn>1097-2765</issn><eissn>1097-4164</eissn><abstract>During
Drosophila embryogenesis, a gradient of Nanos protein emanating from the posterior pole organizes abdominal segmentation. This gradient arises from translational regulation of
nanos mRNA, which is activated in the specialized cytoplasm at the posterior pole of the embryo and repressed elsewhere. Previously, we have defined
cis-acting elements in the mRNA that mediate this translational switch. In this report, we identify a factor named Smaug that binds to these elements and represses translation in the bulk cytoplasm. Smaug interacts genetically and biochemically with Oskar, a key component of the pole plasm for activation of
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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Abdomen Amino Acid Sequence Animals Animals, Genetically Modified Base Sequence Binding Sites Drosophila Drosophila melanogaster - embryology Drosophila melanogaster - genetics Drosophila Proteins Embryo, Nonmammalian - physiology Gene Expression Regulation, Developmental Genomic Library Insect Proteins - genetics Molecular Sequence Data Morphogenesis nanos (nos) gene nanos gene Oskar protein Promoter Regions, Genetic Protein Biosynthesis Recombinant Fusion Proteins - biosynthesis RNA, Messenger - genetics RNA, Messenger - metabolism RNA-Binding Proteins - chemistry RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism Smaug protein |
| title | Smaug, a Novel RNA-Binding Protein that Operates a Translational Switch in Drosophila |
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