Characterization of endothelium-dependent relaxations in mesenteries from transgenic hypertensive rats
Endothelial dysfunction has been reported to be a feature of hypertension. We have investigated the relative contributions of nitric oxide (NO) and the endothelium-derived hyperpolarizing factor (EDHF) to endothelium-dependent relaxations in isolated mesenteries from (mREN-2)-27 transgenic hypertens...
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| Veröffentlicht in: | European journal of pharmacology 1998-09, Vol.358 (1), p.31-40 |
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| description | Endothelial dysfunction has been reported to be a feature of hypertension. We have investigated the relative contributions of nitric oxide (NO) and the endothelium-derived hyperpolarizing factor (EDHF) to endothelium-dependent relaxations in isolated mesenteries from (mREN-2)-27 transgenic hypertensive (TGH) rats and their normotensive controls (Hannover Sprague–Dawley). Relaxation to the endothelium-dependent relaxant, carbachol, was unimpaired in mesenteries from TGH rats compared to the Hannover Sprague–Dawley controls. Inhibition of NO synthase (with 100 μM
N
ω-nitro-
l-arginine methyl ester) had greater inhibitory effects against these relaxations in the mesenteries from Hannover Sprague–Dawley compared to TGH. Inhibition of EDHF activity with high K
+ also had greater inhibitory effects against endothelium-dependent relaxations in the mesenteries from the Hannover Sprague–Dawley compared to TGH. The present results show that, although endothelium-dependent relaxation is unimpaired in mesenteries from TGH rats, there are differences in the relative contributions of NO and EDHF, such that inhibition of either NO or EDHF alone in TGH mesenteries has less impact compared to Hannover Sprague–Dawley. It is suggested that the recently identified reciprocal relationship between NO and EDHF is upregulated in the mesenteries from the TGH rats. |
| doi_str_mv | 10.1016/S0014-2999(98)00584-6 |
| format | Article |
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N
ω-nitro-
l-arginine methyl ester) had greater inhibitory effects against these relaxations in the mesenteries from Hannover Sprague–Dawley compared to TGH. Inhibition of EDHF activity with high K
+ also had greater inhibitory effects against endothelium-dependent relaxations in the mesenteries from the Hannover Sprague–Dawley compared to TGH. The present results show that, although endothelium-dependent relaxation is unimpaired in mesenteries from TGH rats, there are differences in the relative contributions of NO and EDHF, such that inhibition of either NO or EDHF alone in TGH mesenteries has less impact compared to Hannover Sprague–Dawley. It is suggested that the recently identified reciprocal relationship between NO and EDHF is upregulated in the mesenteries from the TGH rats.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/S0014-2999(98)00584-6</identifier><identifier>PMID: 9809866</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Animals, Genetically Modified ; Arterial hypertension. Arterial hypotension ; Biological and medical sciences ; Blood and lymphatic vessels ; Carbachol - pharmacology ; Cardiology. Vascular system ; Cardiotonic Agents - pharmacology ; Cardiovascular Physiological Phenomena - drug effects ; Cromakalim - pharmacology ; EDHF (endothelium-derived hyperpolarizing factor) ; Endothelium ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - physiology ; Enzyme Inhibitors - pharmacology ; Experimental diseases ; Female ; Hypertension ; In Vitro Techniques ; Male ; Medical sciences ; Mesenteric arterial bed ; Mesenteric Arteries - drug effects ; Mesenteric Arteries - physiology ; Methoxamine - pharmacology ; mRen-2 Transgenic hypertensive rat ; NG-Nitroarginine Methyl Ester - pharmacology ; Nitric oxide (NO) ; Nitroprusside - pharmacology ; Potassium Chloride - pharmacology ; Rats ; Rats, Sprague-Dawley ; Vasoconstrictor Agents - pharmacology ; Vasodilation - drug effects ; Vasodilation - physiology ; Vasodilator Agents - pharmacology</subject><ispartof>European journal of pharmacology, 1998-09, Vol.358 (1), p.31-40</ispartof><rights>1998 Elsevier Science B.V.</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-a5a8e23217e0290d44d7cb19e3b0d41f4466eea540a64602ac2204a075c9edd23</citedby><cites>FETCH-LOGICAL-c389t-a5a8e23217e0290d44d7cb19e3b0d41f4466eea540a64602ac2204a075c9edd23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014299998005846$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2436684$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9809866$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Randall, Michael D.</creatorcontrib><creatorcontrib>March, Julie E.</creatorcontrib><title>Characterization of endothelium-dependent relaxations in mesenteries from transgenic hypertensive rats</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>Endothelial dysfunction has been reported to be a feature of hypertension. We have investigated the relative contributions of nitric oxide (NO) and the endothelium-derived hyperpolarizing factor (EDHF) to endothelium-dependent relaxations in isolated mesenteries from (mREN-2)-27 transgenic hypertensive (TGH) rats and their normotensive controls (Hannover Sprague–Dawley). Relaxation to the endothelium-dependent relaxant, carbachol, was unimpaired in mesenteries from TGH rats compared to the Hannover Sprague–Dawley controls. Inhibition of NO synthase (with 100 μM
N
ω-nitro-
l-arginine methyl ester) had greater inhibitory effects against these relaxations in the mesenteries from Hannover Sprague–Dawley compared to TGH. Inhibition of EDHF activity with high K
+ also had greater inhibitory effects against endothelium-dependent relaxations in the mesenteries from the Hannover Sprague–Dawley compared to TGH. The present results show that, although endothelium-dependent relaxation is unimpaired in mesenteries from TGH rats, there are differences in the relative contributions of NO and EDHF, such that inhibition of either NO or EDHF alone in TGH mesenteries has less impact compared to Hannover Sprague–Dawley. It is suggested that the recently identified reciprocal relationship between NO and EDHF is upregulated in the mesenteries from the TGH rats.</description><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Arterial hypertension. Arterial hypotension</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Carbachol - pharmacology</subject><subject>Cardiology. Vascular system</subject><subject>Cardiotonic Agents - pharmacology</subject><subject>Cardiovascular Physiological Phenomena - drug effects</subject><subject>Cromakalim - pharmacology</subject><subject>EDHF (endothelium-derived hyperpolarizing factor)</subject><subject>Endothelium</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - physiology</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Experimental diseases</subject><subject>Female</subject><subject>Hypertension</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mesenteric arterial bed</subject><subject>Mesenteric Arteries - drug effects</subject><subject>Mesenteric Arteries - physiology</subject><subject>Methoxamine - pharmacology</subject><subject>mRen-2 Transgenic hypertensive rat</subject><subject>NG-Nitroarginine Methyl Ester - pharmacology</subject><subject>Nitric oxide (NO)</subject><subject>Nitroprusside - pharmacology</subject><subject>Potassium Chloride - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Vasoconstrictor Agents - pharmacology</subject><subject>Vasodilation - drug effects</subject><subject>Vasodilation - physiology</subject><subject>Vasodilator Agents - pharmacology</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFuEzEQhi0EKmnhESr5gFB7WBh7vV77hKoI2kqVOABny7FnG1e73mA7VcvT4yZRrj1Z4_n-mdFHyDmDLwyY_PoLgImGa60vtLoE6JRo5BuyYKrXDfSMvyWLI_KenOb8AJXSvDshJ1qBVlIuyLBc22RdwRT-2RLmSOeBYvRzWeMYtlPjcVNLjIUmHO3Tjsk0RDphrr81h5kOaZ5oSTbme4zB0fXzBlPBmMMj0mRL_kDeDXbM-PHwnpE_P77_Xt40dz-vb5dXd41rlS6N7axC3nLWI3ANXgjfuxXT2K5qwQYhpES0nQArhQRuHecgLPSd0-g9b8_I5_3cTZr_bjEXM4XscBxtxHmbTQ8gRN_2Fez2oEtzzgkHs0lhsunZMDAvfs3Or3mRZ7QyO79G1tz5YcF2NaE_pg5Ca__ToW-zs-NQnbiQjxgXrZRKVOzbHsMq4zFgMtkFjA59SOiK8XN45ZD_k0eZiw</recordid><startdate>19980925</startdate><enddate>19980925</enddate><creator>Randall, Michael D.</creator><creator>March, Julie E.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980925</creationdate><title>Characterization of endothelium-dependent relaxations in mesenteries from transgenic hypertensive rats</title><author>Randall, Michael D. ; March, Julie E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-a5a8e23217e0290d44d7cb19e3b0d41f4466eea540a64602ac2204a075c9edd23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Animals, Genetically Modified</topic><topic>Arterial hypertension. Arterial hypotension</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Carbachol - pharmacology</topic><topic>Cardiology. Vascular system</topic><topic>Cardiotonic Agents - pharmacology</topic><topic>Cardiovascular Physiological Phenomena - drug effects</topic><topic>Cromakalim - pharmacology</topic><topic>EDHF (endothelium-derived hyperpolarizing factor)</topic><topic>Endothelium</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - physiology</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Experimental diseases</topic><topic>Female</topic><topic>Hypertension</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mesenteric arterial bed</topic><topic>Mesenteric Arteries - drug effects</topic><topic>Mesenteric Arteries - physiology</topic><topic>Methoxamine - pharmacology</topic><topic>mRen-2 Transgenic hypertensive rat</topic><topic>NG-Nitroarginine Methyl Ester - pharmacology</topic><topic>Nitric oxide (NO)</topic><topic>Nitroprusside - pharmacology</topic><topic>Potassium Chloride - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Vasoconstrictor Agents - pharmacology</topic><topic>Vasodilation - drug effects</topic><topic>Vasodilation - physiology</topic><topic>Vasodilator Agents - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Randall, Michael D.</creatorcontrib><creatorcontrib>March, Julie E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Randall, Michael D.</au><au>March, Julie E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of endothelium-dependent relaxations in mesenteries from transgenic hypertensive rats</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>1998-09-25</date><risdate>1998</risdate><volume>358</volume><issue>1</issue><spage>31</spage><epage>40</epage><pages>31-40</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>Endothelial dysfunction has been reported to be a feature of hypertension. We have investigated the relative contributions of nitric oxide (NO) and the endothelium-derived hyperpolarizing factor (EDHF) to endothelium-dependent relaxations in isolated mesenteries from (mREN-2)-27 transgenic hypertensive (TGH) rats and their normotensive controls (Hannover Sprague–Dawley). Relaxation to the endothelium-dependent relaxant, carbachol, was unimpaired in mesenteries from TGH rats compared to the Hannover Sprague–Dawley controls. Inhibition of NO synthase (with 100 μM
N
ω-nitro-
l-arginine methyl ester) had greater inhibitory effects against these relaxations in the mesenteries from Hannover Sprague–Dawley compared to TGH. Inhibition of EDHF activity with high K
+ also had greater inhibitory effects against endothelium-dependent relaxations in the mesenteries from the Hannover Sprague–Dawley compared to TGH. The present results show that, although endothelium-dependent relaxation is unimpaired in mesenteries from TGH rats, there are differences in the relative contributions of NO and EDHF, such that inhibition of either NO or EDHF alone in TGH mesenteries has less impact compared to Hannover Sprague–Dawley. It is suggested that the recently identified reciprocal relationship between NO and EDHF is upregulated in the mesenteries from the TGH rats.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>9809866</pmid><doi>10.1016/S0014-2999(98)00584-6</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | European journal of pharmacology, 1998-09, Vol.358 (1), p.31-40 |
| issn | 0014-2999 1879-0712 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Animals, Genetically Modified Arterial hypertension. Arterial hypotension Biological and medical sciences Blood and lymphatic vessels Carbachol - pharmacology Cardiology. Vascular system Cardiotonic Agents - pharmacology Cardiovascular Physiological Phenomena - drug effects Cromakalim - pharmacology EDHF (endothelium-derived hyperpolarizing factor) Endothelium Endothelium, Vascular - drug effects Endothelium, Vascular - physiology Enzyme Inhibitors - pharmacology Experimental diseases Female Hypertension In Vitro Techniques Male Medical sciences Mesenteric arterial bed Mesenteric Arteries - drug effects Mesenteric Arteries - physiology Methoxamine - pharmacology mRen-2 Transgenic hypertensive rat NG-Nitroarginine Methyl Ester - pharmacology Nitric oxide (NO) Nitroprusside - pharmacology Potassium Chloride - pharmacology Rats Rats, Sprague-Dawley Vasoconstrictor Agents - pharmacology Vasodilation - drug effects Vasodilation - physiology Vasodilator Agents - pharmacology |
| title | Characterization of endothelium-dependent relaxations in mesenteries from transgenic hypertensive rats |
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