Partial characterization of the ribonuclease P from Tetrahymena pyriformis

Ribonuclease P activity from infusoria Tetrahymena pyriformis has been isolated and purified more than 1000-fold over cytosol crude extract. Purified tRNA 5′ endonuclease processes in vitro heterologous, substrates, precursors of the human tRNA Tyr and Drosophila melanogaster tRNA Len, exactly at th...

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Veröffentlicht in:Biochimie 1998-07, Vol.80 (7), p.595-604
Hauptverfasser: Vainauskas, Saulius, Stribinskis, Vilius, Padegimas, Linas, Juodka, Benediktas
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container_end_page 604
container_issue 7
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container_title Biochimie
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creator Vainauskas, Saulius
Stribinskis, Vilius
Padegimas, Linas
Juodka, Benediktas
description Ribonuclease P activity from infusoria Tetrahymena pyriformis has been isolated and purified more than 1000-fold over cytosol crude extract. Purified tRNA 5′ endonuclease processes in vitro heterologous, substrates, precursors of the human tRNA Tyr and Drosophila melanogaster tRNA Len, exactly at the 5′ end of the mature molecules. The activity was abolished by micrococcal nuclease and protease treatment indicating that both RNA and protein components are essential for its activity. The most abundant polypeptides in the purified enzyme fractions have molecular masses of about 100, 44 and 35 kDa. The enzyme requires divalent cations for its activity and shows optimal activity in the presence of the low concentrations of the monovalent salts. Substrate structural requirements for the purified enzyme were analyzed with different tRNA precursor models. The analysis of the derivatives of tRNA Leu precursors with altered aminoacyl stem structures reveals that end of the stem is important for substrate 5′ end processing with purified enzyme.
doi_str_mv 10.1016/S0300-9084(98)80012-6
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subjects Animals
Base Sequence
Drosophila melanogaster
Endoribonucleases - chemistry
Endoribonucleases - isolation & purification
Humans
Molecular Sequence Data
Nucleic Acid Conformation
Ribonuclease P
RNA Precursors - chemistry
RNA, Catalytic - chemistry
RNA, Catalytic - isolation & purification
RNA, Transfer, Leu - chemistry
RNA, Transfer, Tyr - chemistry
Substrate Specificity
Tetrahymena pyriformis
Tetrahymena pyriformis - enzymology
tRNA
title Partial characterization of the ribonuclease P from Tetrahymena pyriformis
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