AML1(-/-) embryos do not express certain hematopoiesis-related gene transcripts including those of the PU.1 gene
The AML1 and PEBP2beta/CBFbeta genes encode the DNA-binding and non-binding subunits, respectively, of the heterodimeric transcription factor, PEBP2/CBF. Targeting each gene results in an almost identical phenotype, namely the complete lack of definitive hematopoiesis in the fetal liver on embryonic...
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| Veröffentlicht in: | Oncogene 1998-11, Vol.17 (18), p.2287-2293 |
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| creator | OKADA, H WATANABE, T ITO, Y NODA, T SATAKE, M NIKI, M TAKANO, H CHIBA, N YANAI, N TANI, K HIBINO, H ASANO, S MUCENSKI, M. L |
| description | The AML1 and PEBP2beta/CBFbeta genes encode the DNA-binding and non-binding subunits, respectively, of the heterodimeric transcription factor, PEBP2/CBF. Targeting each gene results in an almost identical phenotype, namely the complete lack of definitive hematopoiesis in the fetal liver on embryonic day 11.5 (E11.5). We examined and compared the expression levels of various hematopoiesis-related genes in wild type embryos and in embryos mutated for AML1 or PEBP2beta/CBFbeta. The RNAs were prepared from the yolk sacs of E9.5 embryos, from the aorta-gonad- mesonephros regions of E11.5 embryos and from the livers of E11.5 embryos and RT-PCR was performed to detect various gene transcripts. Transcripts were detected for most of the hematopoiesis-related genes that encode transcription factors, cytokines and cytokine receptors, even in tissues from homozygously targeted embryos. On the other hand, PU.1 transcripts were never detected in any tissue of AML1(-/-) or PEBP2beta/CBFbeta(-/-) embryos. In addition, transcripts for the Vav, flk-2/flt-3, M-CSF receptor, G-CSF receptor and c-Myb genes were not detected in certain tissues of the (-/-) embryos. The results suggest that the expression of a particular set of hematopoiesis-related genes is closely correlated with the PEBP2/CBF function. |
| doi_str_mv | 10.1038/sj.onc.1202151 |
| format | Article |
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L</creator><creatorcontrib>OKADA, H ; WATANABE, T ; ITO, Y ; NODA, T ; SATAKE, M ; NIKI, M ; TAKANO, H ; CHIBA, N ; YANAI, N ; TANI, K ; HIBINO, H ; ASANO, S ; MUCENSKI, M. L</creatorcontrib><description>The AML1 and PEBP2beta/CBFbeta genes encode the DNA-binding and non-binding subunits, respectively, of the heterodimeric transcription factor, PEBP2/CBF. Targeting each gene results in an almost identical phenotype, namely the complete lack of definitive hematopoiesis in the fetal liver on embryonic day 11.5 (E11.5). We examined and compared the expression levels of various hematopoiesis-related genes in wild type embryos and in embryos mutated for AML1 or PEBP2beta/CBFbeta. The RNAs were prepared from the yolk sacs of E9.5 embryos, from the aorta-gonad- mesonephros regions of E11.5 embryos and from the livers of E11.5 embryos and RT-PCR was performed to detect various gene transcripts. Transcripts were detected for most of the hematopoiesis-related genes that encode transcription factors, cytokines and cytokine receptors, even in tissues from homozygously targeted embryos. On the other hand, PU.1 transcripts were never detected in any tissue of AML1(-/-) or PEBP2beta/CBFbeta(-/-) embryos. In addition, transcripts for the Vav, flk-2/flt-3, M-CSF receptor, G-CSF receptor and c-Myb genes were not detected in certain tissues of the (-/-) embryos. The results suggest that the expression of a particular set of hematopoiesis-related genes is closely correlated with the PEBP2/CBF function.</description><identifier>ISSN: 0950-9232</identifier><identifier>EISSN: 1476-5594</identifier><identifier>DOI: 10.1038/sj.onc.1202151</identifier><identifier>PMID: 9811459</identifier><language>eng</language><publisher>Basingstoke: Nature Publishing</publisher><subject>AML1 protein ; Animals ; Aorta ; Biological and medical sciences ; c-Myb protein ; CBF protein ; Cell physiology ; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes ; Core Binding Factor Alpha 2 Subunit ; Cytokine receptors ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Embryonic and Fetal Development - genetics ; Embryos ; Female ; Fetuses ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental ; Genes ; Granulocyte colony-stimulating factor ; Hematopoiesis - genetics ; Heterozygote ; Homozygote ; Macrophage colony-stimulating factor ; Mice ; Mice, Inbred C57BL ; Molecular and cellular biology ; Oncogenes - genetics ; Phenotypes ; Proto-Oncogene Proteins - genetics ; PU.1 protein ; Trans-Activators - genetics ; Transcription Factor AP-2 ; Transcription factors ; Transcription Factors - genetics ; Transcription Factors - metabolism</subject><ispartof>Oncogene, 1998-11, Vol.17 (18), p.2287-2293</ispartof><rights>1999 INIST-CNRS</rights><rights>Macmillan Publishers Limited 1998.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-4b8fb6588384d68c6d76685a4fe18048aaecc4381f7780ebe76bc04339d7983f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1607070$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9811459$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>OKADA, H</creatorcontrib><creatorcontrib>WATANABE, T</creatorcontrib><creatorcontrib>ITO, Y</creatorcontrib><creatorcontrib>NODA, T</creatorcontrib><creatorcontrib>SATAKE, M</creatorcontrib><creatorcontrib>NIKI, M</creatorcontrib><creatorcontrib>TAKANO, H</creatorcontrib><creatorcontrib>CHIBA, N</creatorcontrib><creatorcontrib>YANAI, N</creatorcontrib><creatorcontrib>TANI, K</creatorcontrib><creatorcontrib>HIBINO, H</creatorcontrib><creatorcontrib>ASANO, S</creatorcontrib><creatorcontrib>MUCENSKI, M. L</creatorcontrib><title>AML1(-/-) embryos do not express certain hematopoiesis-related gene transcripts including those of the PU.1 gene</title><title>Oncogene</title><addtitle>Oncogene</addtitle><description>The AML1 and PEBP2beta/CBFbeta genes encode the DNA-binding and non-binding subunits, respectively, of the heterodimeric transcription factor, PEBP2/CBF. Targeting each gene results in an almost identical phenotype, namely the complete lack of definitive hematopoiesis in the fetal liver on embryonic day 11.5 (E11.5). We examined and compared the expression levels of various hematopoiesis-related genes in wild type embryos and in embryos mutated for AML1 or PEBP2beta/CBFbeta. The RNAs were prepared from the yolk sacs of E9.5 embryos, from the aorta-gonad- mesonephros regions of E11.5 embryos and from the livers of E11.5 embryos and RT-PCR was performed to detect various gene transcripts. Transcripts were detected for most of the hematopoiesis-related genes that encode transcription factors, cytokines and cytokine receptors, even in tissues from homozygously targeted embryos. On the other hand, PU.1 transcripts were never detected in any tissue of AML1(-/-) or PEBP2beta/CBFbeta(-/-) embryos. In addition, transcripts for the Vav, flk-2/flt-3, M-CSF receptor, G-CSF receptor and c-Myb genes were not detected in certain tissues of the (-/-) embryos. The results suggest that the expression of a particular set of hematopoiesis-related genes is closely correlated with the PEBP2/CBF function.</description><subject>AML1 protein</subject><subject>Animals</subject><subject>Aorta</subject><subject>Biological and medical sciences</subject><subject>c-Myb protein</subject><subject>CBF protein</subject><subject>Cell physiology</subject><subject>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</subject><subject>Core Binding Factor Alpha 2 Subunit</subject><subject>Cytokine receptors</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Embryonic and Fetal Development - genetics</subject><subject>Embryos</subject><subject>Female</subject><subject>Fetuses</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genes</subject><subject>Granulocyte colony-stimulating factor</subject><subject>Hematopoiesis - genetics</subject><subject>Heterozygote</subject><subject>Homozygote</subject><subject>Macrophage colony-stimulating factor</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular and cellular biology</subject><subject>Oncogenes - genetics</subject><subject>Phenotypes</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>PU.1 protein</subject><subject>Trans-Activators - genetics</subject><subject>Transcription Factor AP-2</subject><subject>Transcription factors</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><issn>0950-9232</issn><issn>1476-5594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2L1jAQxoMo6-vq1ZsQUEQP7SZNmo_jsvgFr-jBPYc0ne7mpW1qpgX3vze6RcGLzMAMzG8emHkIec5ZzZkwF3iq0xxq3rCGt_wBOXCpVdW2Vj4kB2ZbVtlGNI_JE8QTY0xb1pyRM2s4l609kOXy85G_qS6qtxSmLt8lpH2ic1op_FgyINIAefVxprcw-TUtKQJGrDKMfoWe3sAMdM1-xpDjsiKNcxi3Ps43dL1NCDQNpQH69brmv-Gn5NHgR4Rnez0n1-_ffbv6WB2_fPh0dXmsgtBmrWRnhk61xggje2WC6rVSpvVyAG6YNN5DCFIYPmhtGHSgVReYFML22hoxiHPy-l53yen7Bri6KWKAcfQzpA2dZoVWVv8X5JqXNLKAL_8BT2nLcznCNUpyUTArClXfUyEnxAyDW3KcfL5znLlfjjk8ueKY2x0rCy922a2boP-D7xaV-at97jH4cSi_DhH_qiqmS4if_UidZg</recordid><startdate>19981105</startdate><enddate>19981105</enddate><creator>OKADA, H</creator><creator>WATANABE, T</creator><creator>ITO, Y</creator><creator>NODA, T</creator><creator>SATAKE, M</creator><creator>NIKI, M</creator><creator>TAKANO, H</creator><creator>CHIBA, N</creator><creator>YANAI, N</creator><creator>TANI, K</creator><creator>HIBINO, H</creator><creator>ASANO, S</creator><creator>MUCENSKI, M. L</creator><general>Nature Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19981105</creationdate><title>AML1(-/-) embryos do not express certain hematopoiesis-related gene transcripts including those of the PU.1 gene</title><author>OKADA, H ; WATANABE, T ; ITO, Y ; NODA, T ; SATAKE, M ; NIKI, M ; TAKANO, H ; CHIBA, N ; YANAI, N ; TANI, K ; HIBINO, H ; ASANO, S ; MUCENSKI, M. 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Psychology</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genes</topic><topic>Granulocyte colony-stimulating factor</topic><topic>Hematopoiesis - genetics</topic><topic>Heterozygote</topic><topic>Homozygote</topic><topic>Macrophage colony-stimulating factor</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Molecular and cellular biology</topic><topic>Oncogenes - genetics</topic><topic>Phenotypes</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>PU.1 protein</topic><topic>Trans-Activators - genetics</topic><topic>Transcription Factor AP-2</topic><topic>Transcription factors</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OKADA, H</creatorcontrib><creatorcontrib>WATANABE, T</creatorcontrib><creatorcontrib>ITO, Y</creatorcontrib><creatorcontrib>NODA, T</creatorcontrib><creatorcontrib>SATAKE, M</creatorcontrib><creatorcontrib>NIKI, M</creatorcontrib><creatorcontrib>TAKANO, H</creatorcontrib><creatorcontrib>CHIBA, N</creatorcontrib><creatorcontrib>YANAI, N</creatorcontrib><creatorcontrib>TANI, K</creatorcontrib><creatorcontrib>HIBINO, H</creatorcontrib><creatorcontrib>ASANO, S</creatorcontrib><creatorcontrib>MUCENSKI, M. 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L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>AML1(-/-) embryos do not express certain hematopoiesis-related gene transcripts including those of the PU.1 gene</atitle><jtitle>Oncogene</jtitle><addtitle>Oncogene</addtitle><date>1998-11-05</date><risdate>1998</risdate><volume>17</volume><issue>18</issue><spage>2287</spage><epage>2293</epage><pages>2287-2293</pages><issn>0950-9232</issn><eissn>1476-5594</eissn><abstract>The AML1 and PEBP2beta/CBFbeta genes encode the DNA-binding and non-binding subunits, respectively, of the heterodimeric transcription factor, PEBP2/CBF. Targeting each gene results in an almost identical phenotype, namely the complete lack of definitive hematopoiesis in the fetal liver on embryonic day 11.5 (E11.5). We examined and compared the expression levels of various hematopoiesis-related genes in wild type embryos and in embryos mutated for AML1 or PEBP2beta/CBFbeta. The RNAs were prepared from the yolk sacs of E9.5 embryos, from the aorta-gonad- mesonephros regions of E11.5 embryos and from the livers of E11.5 embryos and RT-PCR was performed to detect various gene transcripts. Transcripts were detected for most of the hematopoiesis-related genes that encode transcription factors, cytokines and cytokine receptors, even in tissues from homozygously targeted embryos. On the other hand, PU.1 transcripts were never detected in any tissue of AML1(-/-) or PEBP2beta/CBFbeta(-/-) embryos. In addition, transcripts for the Vav, flk-2/flt-3, M-CSF receptor, G-CSF receptor and c-Myb genes were not detected in certain tissues of the (-/-) embryos. The results suggest that the expression of a particular set of hematopoiesis-related genes is closely correlated with the PEBP2/CBF function.</abstract><cop>Basingstoke</cop><pub>Nature Publishing</pub><pmid>9811459</pmid><doi>10.1038/sj.onc.1202151</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0950-9232 |
| ispartof | Oncogene, 1998-11, Vol.17 (18), p.2287-2293 |
| issn | 0950-9232 1476-5594 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature Journals Online; EZB-FREE-00999 freely available EZB journals |
| subjects | AML1 protein Animals Aorta Biological and medical sciences c-Myb protein CBF protein Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Core Binding Factor Alpha 2 Subunit Cytokine receptors DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Embryonic and Fetal Development - genetics Embryos Female Fetuses Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental Genes Granulocyte colony-stimulating factor Hematopoiesis - genetics Heterozygote Homozygote Macrophage colony-stimulating factor Mice Mice, Inbred C57BL Molecular and cellular biology Oncogenes - genetics Phenotypes Proto-Oncogene Proteins - genetics PU.1 protein Trans-Activators - genetics Transcription Factor AP-2 Transcription factors Transcription Factors - genetics Transcription Factors - metabolism |
| title | AML1(-/-) embryos do not express certain hematopoiesis-related gene transcripts including those of the PU.1 gene |
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