Kinetics of antibody-based antigen detection in serum and faeces of sheep experimentally infected with Fasciola hepatica
The monoclonal antibody ES78 was used in a sandwich immunosorbent assay (Sandwich ELISA) for the detection of antigens in sera and faeces in the course of Fasciola hepatica infection in 10 experimentally infected sheep.. All infected sheep had circulating antigens in the first week post-infection (W...
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| Veröffentlicht in: | Veterinary parasitology 1999-09, Vol.86 (1), p.23-31 |
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| creator | Duménigo, B.E. Espino, A.M. Finlay, C.M. Mezo, M. |
| description | The monoclonal antibody ES78 was used in a sandwich immunosorbent assay (Sandwich ELISA) for the detection of antigens in sera and faeces in the course of
Fasciola hepatica infection in 10 experimentally infected sheep.. All infected sheep had circulating antigens in the first week post-infection (WPI). Antigenemia was detectable until WPI 3 in four infected sheep, WPI 4 in five infected sheep and in only one sheep by WPI 5. The detection of coproantigens (Fa
g) was possible in five infected sheep at WPI-4, in four sheep at WPI-5 and in one sheep only at WPI-6. This technique was compared to an indirect ELISA for the detection of antibodies using excretory secretory antigens of
F. hepatica. A significant correlation was found between Fa
g and egg output and also with adult worm numbers. Our method demonstrated that the diagnosis of active fasciolosis in sheep is possible during all periods of infection. |
| doi_str_mv | 10.1016/S0304-4017(99)00126-0 |
| format | Article |
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Fasciola hepatica infection in 10 experimentally infected sheep.. All infected sheep had circulating antigens in the first week post-infection (WPI). Antigenemia was detectable until WPI 3 in four infected sheep, WPI 4 in five infected sheep and in only one sheep by WPI 5. The detection of coproantigens (Fa
g) was possible in five infected sheep at WPI-4, in four sheep at WPI-5 and in one sheep only at WPI-6. This technique was compared to an indirect ELISA for the detection of antibodies using excretory secretory antigens of
F. hepatica. A significant correlation was found between Fa
g and egg output and also with adult worm numbers. Our method demonstrated that the diagnosis of active fasciolosis in sheep is possible during all periods of infection.</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/S0304-4017(99)00126-0</identifier><identifier>PMID: 10489199</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antibodies, Helminth - blood ; Antibodies, Monoclonal - immunology ; antibody detection ; ANTICORPS MONOCLONAL ; ANTICUERPOS MONOCLONALES ; antigen detection ; ANTIGENE ; antigenemia ; ANTIGENOS ; ANTIGENS ; Antigens, Helminth - analysis ; Antigens, Helminth - blood ; Antigens, Helminth - immunology ; BLOOD SERUM ; Cattle: Trematoda ; Circulating antigen ; circulating antigens ; Coproantigen ; coproantigens ; ELISA ; enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Enzyme-Linked Immunosorbent Assay - veterinary ; ES antigen detection ; excretory-secretory products ; experimental infections ; FAECES ; FASCIOLA HEPATICA ; Fasciola hepatica - immunology ; Fasciola hepatica - isolation & purification ; fascioliasis ; Fascioliasis - diagnosis ; Fascioliasis - immunology ; Fascioliasis - veterinary ; fecal egg count ; FECES ; Feces - parasitology ; Female ; HECES ; helminth ova ; Kinetics ; Kinetics, sandwich ELISA ; MONOCLONAL ANTIBODIES ; OVIN ; OVINOS ; Parasite Egg Count - veterinary ; serodiagnosis ; SERUM SANGUIN ; SHEEP ; Sheep Diseases - diagnosis ; Sheep Diseases - immunology ; Sheep Diseases - parasitology ; SUERO SANGUINEO ; temporal variation ; TEST ELISA</subject><ispartof>Veterinary parasitology, 1999-09, Vol.86 (1), p.23-31</ispartof><rights>1999 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-8d306c5346f26a27bfd77ae682be350922a57d0a815bc69e7473e5d3f40d4bf73</citedby><cites>FETCH-LOGICAL-c407t-8d306c5346f26a27bfd77ae682be350922a57d0a815bc69e7473e5d3f40d4bf73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304401799001260$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10489199$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Duménigo, B.E.</creatorcontrib><creatorcontrib>Espino, A.M.</creatorcontrib><creatorcontrib>Finlay, C.M.</creatorcontrib><creatorcontrib>Mezo, M.</creatorcontrib><title>Kinetics of antibody-based antigen detection in serum and faeces of sheep experimentally infected with Fasciola hepatica</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>The monoclonal antibody ES78 was used in a sandwich immunosorbent assay (Sandwich ELISA) for the detection of antigens in sera and faeces in the course of
Fasciola hepatica infection in 10 experimentally infected sheep.. All infected sheep had circulating antigens in the first week post-infection (WPI). Antigenemia was detectable until WPI 3 in four infected sheep, WPI 4 in five infected sheep and in only one sheep by WPI 5. The detection of coproantigens (Fa
g) was possible in five infected sheep at WPI-4, in four sheep at WPI-5 and in one sheep only at WPI-6. This technique was compared to an indirect ELISA for the detection of antibodies using excretory secretory antigens of
F. hepatica. A significant correlation was found between Fa
g and egg output and also with adult worm numbers. Our method demonstrated that the diagnosis of active fasciolosis in sheep is possible during all periods of infection.</description><subject>Animals</subject><subject>Antibodies, Helminth - blood</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>antibody detection</subject><subject>ANTICORPS MONOCLONAL</subject><subject>ANTICUERPOS MONOCLONALES</subject><subject>antigen detection</subject><subject>ANTIGENE</subject><subject>antigenemia</subject><subject>ANTIGENOS</subject><subject>ANTIGENS</subject><subject>Antigens, Helminth - analysis</subject><subject>Antigens, Helminth - blood</subject><subject>Antigens, Helminth - immunology</subject><subject>BLOOD SERUM</subject><subject>Cattle: Trematoda</subject><subject>Circulating antigen</subject><subject>circulating antigens</subject><subject>Coproantigen</subject><subject>coproantigens</subject><subject>ELISA</subject><subject>enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>ES antigen detection</subject><subject>excretory-secretory products</subject><subject>experimental infections</subject><subject>FAECES</subject><subject>FASCIOLA HEPATICA</subject><subject>Fasciola hepatica - immunology</subject><subject>Fasciola hepatica - isolation & purification</subject><subject>fascioliasis</subject><subject>Fascioliasis - diagnosis</subject><subject>Fascioliasis - immunology</subject><subject>Fascioliasis - veterinary</subject><subject>fecal egg count</subject><subject>FECES</subject><subject>Feces - parasitology</subject><subject>Female</subject><subject>HECES</subject><subject>helminth ova</subject><subject>Kinetics</subject><subject>Kinetics, sandwich ELISA</subject><subject>MONOCLONAL ANTIBODIES</subject><subject>OVIN</subject><subject>OVINOS</subject><subject>Parasite Egg Count - veterinary</subject><subject>serodiagnosis</subject><subject>SERUM SANGUIN</subject><subject>SHEEP</subject><subject>Sheep Diseases - diagnosis</subject><subject>Sheep Diseases - immunology</subject><subject>Sheep Diseases - parasitology</subject><subject>SUERO SANGUINEO</subject><subject>temporal variation</subject><subject>TEST ELISA</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi0EokvhJxR8QuUQGH_F8alCVQuIFRxKz5Zjj7tG2STEWej-e7ybCiEu-GJZ87wz44eQMwZvGbD63Q0IkJUEps-NeQPAeF3BI7JijRYVVwoek9Uf5IQ8y_k7AEio9VNywkA2hhmzIvefU49z8pkOkbp-Tu0Q9lXrMobj8w57GnBGP6ehp6mnGafdtpQCjQ49HnN5gzhSvB9xSlvsZ9d1-8LGkiptfqV5Q69d9mnoHN3g6Mo895w8ia7L-OLhPiW311ffLj9W668fPl2-X1degp6rJgiovRKyjrx2XLcxaO2wbniLQoHh3CkdwDVMtb42qKUWqIKIEoJsoxan5PXSd5yGHzvMs92m7LHrXI_DLlsNIBojWQHVAvppyHnCaMfyGzftLQN7UG6Pyu3BpzXGHpVbKLmXDwN27RbDX6nFcQFeLUB0g3V3U8r29oYDE8CNFFwdWpz9S3xZczgcJlVT6hdLHYuonwknW2Ri7zGkqTi2YUj_WfI3gWKjwQ</recordid><startdate>19990915</startdate><enddate>19990915</enddate><creator>Duménigo, B.E.</creator><creator>Espino, A.M.</creator><creator>Finlay, C.M.</creator><creator>Mezo, M.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990915</creationdate><title>Kinetics of antibody-based antigen detection in serum and faeces of sheep experimentally infected with Fasciola hepatica</title><author>Duménigo, B.E. ; Espino, A.M. ; Finlay, C.M. ; Mezo, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-8d306c5346f26a27bfd77ae682be350922a57d0a815bc69e7473e5d3f40d4bf73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Antibodies, Helminth - blood</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>antibody detection</topic><topic>ANTICORPS MONOCLONAL</topic><topic>ANTICUERPOS MONOCLONALES</topic><topic>antigen detection</topic><topic>ANTIGENE</topic><topic>antigenemia</topic><topic>ANTIGENOS</topic><topic>ANTIGENS</topic><topic>Antigens, Helminth - analysis</topic><topic>Antigens, Helminth - blood</topic><topic>Antigens, Helminth - immunology</topic><topic>BLOOD SERUM</topic><topic>Cattle: Trematoda</topic><topic>Circulating antigen</topic><topic>circulating antigens</topic><topic>Coproantigen</topic><topic>coproantigens</topic><topic>ELISA</topic><topic>enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>ES antigen detection</topic><topic>excretory-secretory products</topic><topic>experimental infections</topic><topic>FAECES</topic><topic>FASCIOLA HEPATICA</topic><topic>Fasciola hepatica - immunology</topic><topic>Fasciola hepatica - isolation & purification</topic><topic>fascioliasis</topic><topic>Fascioliasis - diagnosis</topic><topic>Fascioliasis - immunology</topic><topic>Fascioliasis - veterinary</topic><topic>fecal egg count</topic><topic>FECES</topic><topic>Feces - parasitology</topic><topic>Female</topic><topic>HECES</topic><topic>helminth ova</topic><topic>Kinetics</topic><topic>Kinetics, sandwich ELISA</topic><topic>MONOCLONAL ANTIBODIES</topic><topic>OVIN</topic><topic>OVINOS</topic><topic>Parasite Egg Count - veterinary</topic><topic>serodiagnosis</topic><topic>SERUM SANGUIN</topic><topic>SHEEP</topic><topic>Sheep Diseases - diagnosis</topic><topic>Sheep Diseases - immunology</topic><topic>Sheep Diseases - parasitology</topic><topic>SUERO SANGUINEO</topic><topic>temporal variation</topic><topic>TEST ELISA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Duménigo, B.E.</creatorcontrib><creatorcontrib>Espino, A.M.</creatorcontrib><creatorcontrib>Finlay, C.M.</creatorcontrib><creatorcontrib>Mezo, M.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duménigo, B.E.</au><au>Espino, A.M.</au><au>Finlay, C.M.</au><au>Mezo, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Kinetics of antibody-based antigen detection in serum and faeces of sheep experimentally infected with Fasciola hepatica</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>1999-09-15</date><risdate>1999</risdate><volume>86</volume><issue>1</issue><spage>23</spage><epage>31</epage><pages>23-31</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>The monoclonal antibody ES78 was used in a sandwich immunosorbent assay (Sandwich ELISA) for the detection of antigens in sera and faeces in the course of
Fasciola hepatica infection in 10 experimentally infected sheep.. All infected sheep had circulating antigens in the first week post-infection (WPI). Antigenemia was detectable until WPI 3 in four infected sheep, WPI 4 in five infected sheep and in only one sheep by WPI 5. The detection of coproantigens (Fa
g) was possible in five infected sheep at WPI-4, in four sheep at WPI-5 and in one sheep only at WPI-6. This technique was compared to an indirect ELISA for the detection of antibodies using excretory secretory antigens of
F. hepatica. A significant correlation was found between Fa
g and egg output and also with adult worm numbers. Our method demonstrated that the diagnosis of active fasciolosis in sheep is possible during all periods of infection.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>10489199</pmid><doi>10.1016/S0304-4017(99)00126-0</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-4017 |
| ispartof | Veterinary parasitology, 1999-09, Vol.86 (1), p.23-31 |
| issn | 0304-4017 1873-2550 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibodies, Helminth - blood Antibodies, Monoclonal - immunology antibody detection ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES antigen detection ANTIGENE antigenemia ANTIGENOS ANTIGENS Antigens, Helminth - analysis Antigens, Helminth - blood Antigens, Helminth - immunology BLOOD SERUM Cattle: Trematoda Circulating antigen circulating antigens Coproantigen coproantigens ELISA enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary ES antigen detection excretory-secretory products experimental infections FAECES FASCIOLA HEPATICA Fasciola hepatica - immunology Fasciola hepatica - isolation & purification fascioliasis Fascioliasis - diagnosis Fascioliasis - immunology Fascioliasis - veterinary fecal egg count FECES Feces - parasitology Female HECES helminth ova Kinetics Kinetics, sandwich ELISA MONOCLONAL ANTIBODIES OVIN OVINOS Parasite Egg Count - veterinary serodiagnosis SERUM SANGUIN SHEEP Sheep Diseases - diagnosis Sheep Diseases - immunology Sheep Diseases - parasitology SUERO SANGUINEO temporal variation TEST ELISA |
| title | Kinetics of antibody-based antigen detection in serum and faeces of sheep experimentally infected with Fasciola hepatica |
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