Determining chemotactic responses by two subsurface microaerophiles using a simplified capillary assay method
A simplified capillary chemotaxis assay utilizing a hypodermic needle, syringe, and disposable pipette tip was developed to measure bacterial tactic responses. The method was applied to two strains of subsurface microaerophilic bacteria. This method was more convenient than the Adler method and requ...
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| Veröffentlicht in: | Journal of microbiological methods 1999-09, Vol.37 (3), p.255-263 |
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| container_title | Journal of microbiological methods |
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| creator | Mazumder, Raja Phelps, Tommy J Krieg, Noel R Benoit, Robert E |
| description | A simplified capillary chemotaxis assay utilizing a hypodermic needle, syringe, and disposable pipette tip was developed to measure bacterial tactic responses. The method was applied to two strains of subsurface microaerophilic bacteria. This method was more convenient than the Adler method and required less practice. Isolate VT10 was a strain of
Pseudomonas syringae, which was isolated from the shallow subsurface. It was chemotactically attracted toward dextrose, glycerol, and phenol, which could be used as sole carbon sources, and toward maltose, which could not be used. Isolate MR100 was phylogenetically related to
Pseudomonas mendocina and was isolated from the deep subsurface. It showed no tactic response to these compounds, although, it could use dextrose, maltose, and glycerol as carbon sources. The chemotaxis results obtained by the new method were verified by using the swarm plate assay technique. The simplified technique may be useful for routine chemotactic testing. |
| doi_str_mv | 10.1016/S0167-7012(99)00072-X |
| format | Article |
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Pseudomonas syringae, which was isolated from the shallow subsurface. It was chemotactically attracted toward dextrose, glycerol, and phenol, which could be used as sole carbon sources, and toward maltose, which could not be used. Isolate MR100 was phylogenetically related to
Pseudomonas mendocina and was isolated from the deep subsurface. It showed no tactic response to these compounds, although, it could use dextrose, maltose, and glycerol as carbon sources. The chemotaxis results obtained by the new method were verified by using the swarm plate assay technique. The simplified technique may be useful for routine chemotactic testing.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/S0167-7012(99)00072-X</identifier><identifier>PMID: 10480269</identifier><identifier>CODEN: JMIMDQ</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Bacteriological methods and techniques used in bacteriology ; Bacteriological Techniques ; Bacteriology ; Biological and medical sciences ; Chemotaxis ; Culture Media - chemistry ; Fundamental and applied biological sciences. Psychology ; Glucose - metabolism ; Glycerol - metabolism ; Microaerophilic ; Microbiology ; Pseudomonas - physiology ; Simplified capillary chemotaxis assay ; Subsurface ; Swarm plate assay</subject><ispartof>Journal of microbiological methods, 1999-09, Vol.37 (3), p.255-263</ispartof><rights>1999 Elsevier Science B.V.</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-ad96f33518f4d4b3c3db120ecab3f60224147fdf24cb029d0c81cce633df015b3</citedby><cites>FETCH-LOGICAL-c421t-ad96f33518f4d4b3c3db120ecab3f60224147fdf24cb029d0c81cce633df015b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0167-7012(99)00072-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1910888$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10480269$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mazumder, Raja</creatorcontrib><creatorcontrib>Phelps, Tommy J</creatorcontrib><creatorcontrib>Krieg, Noel R</creatorcontrib><creatorcontrib>Benoit, Robert E</creatorcontrib><title>Determining chemotactic responses by two subsurface microaerophiles using a simplified capillary assay method</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>A simplified capillary chemotaxis assay utilizing a hypodermic needle, syringe, and disposable pipette tip was developed to measure bacterial tactic responses. The method was applied to two strains of subsurface microaerophilic bacteria. This method was more convenient than the Adler method and required less practice. Isolate VT10 was a strain of
Pseudomonas syringae, which was isolated from the shallow subsurface. It was chemotactically attracted toward dextrose, glycerol, and phenol, which could be used as sole carbon sources, and toward maltose, which could not be used. Isolate MR100 was phylogenetically related to
Pseudomonas mendocina and was isolated from the deep subsurface. It showed no tactic response to these compounds, although, it could use dextrose, maltose, and glycerol as carbon sources. The chemotaxis results obtained by the new method were verified by using the swarm plate assay technique. The simplified technique may be useful for routine chemotactic testing.</description><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriological Techniques</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Chemotaxis</subject><subject>Culture Media - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose - metabolism</subject><subject>Glycerol - metabolism</subject><subject>Microaerophilic</subject><subject>Microbiology</subject><subject>Pseudomonas - physiology</subject><subject>Simplified capillary chemotaxis assay</subject><subject>Subsurface</subject><subject>Swarm plate assay</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctuFDEQRS0EIpPAJ4C8QAgWDX70dNsrhEJ4SJFYAFJ2lrtcZoz6hasbNH-PJzMCdtm4vDi3qm5dxp5I8UoK2bz-Up62aoVUL6x9KYRoVXVzj22kKR-jt_Y-2_xFztg50Q8h5FbX5iE7k6I2QjV2w4Z3uGAe0pjG7xx2OEyLhyUBz0jzNBIS7_Z8-T1xWjtac_SAfEiQJ495mnepL8RKB7XnlIa5TzFh4ODn1Pc-77kn8ns-4LKbwiP2IPqe8PGpXrBv76--Xn6srj9_-HT59rqCWsml8sE2UeutNLEOdadBh04qgeA7HRuhVC3rNoaoauiEskGAkQDYaB1i8djpC_b82HfO088VaXFDIsCy0IjTSq4VQmtr7J2gbFWrTa0LuD2CxTlRxujmnIbiz0nhDoG420Dc4drOWncbiLspuqenAWs3YPhPdUygAM9OgCfwfcx-hET_OCuFMaZgb44YlrP9SpgdQcIRMKSMsLgwpTs2-QOfA6pF</recordid><startdate>19990901</startdate><enddate>19990901</enddate><creator>Mazumder, Raja</creator><creator>Phelps, Tommy J</creator><creator>Krieg, Noel R</creator><creator>Benoit, Robert E</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19990901</creationdate><title>Determining chemotactic responses by two subsurface microaerophiles using a simplified capillary assay method</title><author>Mazumder, Raja ; Phelps, Tommy J ; Krieg, Noel R ; Benoit, Robert E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-ad96f33518f4d4b3c3db120ecab3f60224147fdf24cb029d0c81cce633df015b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriological Techniques</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Chemotaxis</topic><topic>Culture Media - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - metabolism</topic><topic>Glycerol - metabolism</topic><topic>Microaerophilic</topic><topic>Microbiology</topic><topic>Pseudomonas - physiology</topic><topic>Simplified capillary chemotaxis assay</topic><topic>Subsurface</topic><topic>Swarm plate assay</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mazumder, Raja</creatorcontrib><creatorcontrib>Phelps, Tommy J</creatorcontrib><creatorcontrib>Krieg, Noel R</creatorcontrib><creatorcontrib>Benoit, Robert E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mazumder, Raja</au><au>Phelps, Tommy J</au><au>Krieg, Noel R</au><au>Benoit, Robert E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determining chemotactic responses by two subsurface microaerophiles using a simplified capillary assay method</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>1999-09-01</date><risdate>1999</risdate><volume>37</volume><issue>3</issue><spage>255</spage><epage>263</epage><pages>255-263</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><coden>JMIMDQ</coden><abstract>A simplified capillary chemotaxis assay utilizing a hypodermic needle, syringe, and disposable pipette tip was developed to measure bacterial tactic responses. 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Pseudomonas syringae, which was isolated from the shallow subsurface. It was chemotactically attracted toward dextrose, glycerol, and phenol, which could be used as sole carbon sources, and toward maltose, which could not be used. Isolate MR100 was phylogenetically related to
Pseudomonas mendocina and was isolated from the deep subsurface. It showed no tactic response to these compounds, although, it could use dextrose, maltose, and glycerol as carbon sources. The chemotaxis results obtained by the new method were verified by using the swarm plate assay technique. The simplified technique may be useful for routine chemotactic testing.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>10480269</pmid><doi>10.1016/S0167-7012(99)00072-X</doi><tpages>9</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Bacteriological methods and techniques used in bacteriology Bacteriological Techniques Bacteriology Biological and medical sciences Chemotaxis Culture Media - chemistry Fundamental and applied biological sciences. Psychology Glucose - metabolism Glycerol - metabolism Microaerophilic Microbiology Pseudomonas - physiology Simplified capillary chemotaxis assay Subsurface Swarm plate assay |
| title | Determining chemotactic responses by two subsurface microaerophiles using a simplified capillary assay method |
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