Green fluorescent protein (GFP) as a reporter gene for the plant pathogenic oomycete Phytophthora palmivora
Transgenic Phytophthora palmivora strains that produce green fluorescent protein (GFP) or β-glucuronidase (GUS) constitutively were obtained after stable DNA integration using a polyethylene-glycol and CaCl 2-based transformation protocol. GFP and GUS production were monitored during several stages...
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| Veröffentlicht in: | FEMS microbiology letters 1999-09, Vol.178 (1), p.71-80 |
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| container_title | FEMS microbiology letters |
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| creator | van West, Pieter Reid, Brian Campbell, Tracey A. Sandrock, Robert W. Fry, William E. Kamoun, Sophien Gow, Neil A.R. |
| description | Transgenic
Phytophthora palmivora strains that produce green fluorescent protein (GFP) or β-glucuronidase (GUS) constitutively were obtained after stable DNA integration using a polyethylene-glycol and CaCl
2-based transformation protocol. GFP and GUS production were monitored during several stages of the life cycle of
P. palmivora to evaluate their use in molecular and physiological studies. 40% of the GFP transformants produced the GFP to a level detectable by a confocal laser scanning microscope, whereas 75% of the GUS transformants produced GUS. GFP could be visualised readily in swimming zoospores and other developmental stages of
P. palmivora cells. For high magnification microscopic studies, GFP is better visualised and was superior to GUS. In contrast, for macroscopic examination, GUS was superior. Our findings indicate that both GFP and GUS can be used successfully as reporter genes in
P. palmivora. |
| doi_str_mv | 10.1016/S0378-1097(99)00320-1 |
| format | Article |
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Phytophthora palmivora strains that produce green fluorescent protein (GFP) or β-glucuronidase (GUS) constitutively were obtained after stable DNA integration using a polyethylene-glycol and CaCl
2-based transformation protocol. GFP and GUS production were monitored during several stages of the life cycle of
P. palmivora to evaluate their use in molecular and physiological studies. 40% of the GFP transformants produced the GFP to a level detectable by a confocal laser scanning microscope, whereas 75% of the GUS transformants produced GUS. GFP could be visualised readily in swimming zoospores and other developmental stages of
P. palmivora cells. For high magnification microscopic studies, GFP is better visualised and was superior to GUS. In contrast, for macroscopic examination, GUS was superior. Our findings indicate that both GFP and GUS can be used successfully as reporter genes in
P. palmivora.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/S0378-1097(99)00320-1</identifier><identifier>PMID: 10483725</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford: Elsevier B.V</publisher><subject>Algal Proteins - genetics ; Algal Proteins - metabolism ; Animals ; b-Glucuronidase ; Biological and medical sciences ; calcium chloride ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; Generalities. Techniques ; Genes, Reporter ; genetic transformation ; Glucuronidase - genetics ; Glucuronidase - metabolism ; Green fluorescent protein ; Green Fluorescent Proteins ; GUS protein ; Life Cycle Stages ; Luminescent Proteins - genetics ; Luminescent Proteins - metabolism ; Microbiology ; Microscopy, Confocal ; Microscopy, Fluorescence ; Mycological methods and techniques used in mycology ; Mycology ; Oomycete ; Phytopathology. Animal pests. Plant and forest protection ; Phytophthora - chemistry ; Phytophthora - enzymology ; Phytophthora - genetics ; Phytophthora - growth & development ; Phytophthora palmivora ; plant pathogenic fungi ; Plants - microbiology ; polyethylene glycol ; Scyphozoa ; Transformation, Genetic ; β-Glucuronidase</subject><ispartof>FEMS microbiology letters, 1999-09, Vol.178 (1), p.71-80</ispartof><rights>1999 Federation of European Microbiological Societies</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c563t-99a6bcba009ebd5fca6f66bd55a6c804db1aa59f36bc5519647c2ec32176f6683</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1912235$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10483725$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van West, Pieter</creatorcontrib><creatorcontrib>Reid, Brian</creatorcontrib><creatorcontrib>Campbell, Tracey A.</creatorcontrib><creatorcontrib>Sandrock, Robert W.</creatorcontrib><creatorcontrib>Fry, William E.</creatorcontrib><creatorcontrib>Kamoun, Sophien</creatorcontrib><creatorcontrib>Gow, Neil A.R.</creatorcontrib><title>Green fluorescent protein (GFP) as a reporter gene for the plant pathogenic oomycete Phytophthora palmivora</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Transgenic
Phytophthora palmivora strains that produce green fluorescent protein (GFP) or β-glucuronidase (GUS) constitutively were obtained after stable DNA integration using a polyethylene-glycol and CaCl
2-based transformation protocol. GFP and GUS production were monitored during several stages of the life cycle of
P. palmivora to evaluate their use in molecular and physiological studies. 40% of the GFP transformants produced the GFP to a level detectable by a confocal laser scanning microscope, whereas 75% of the GUS transformants produced GUS. GFP could be visualised readily in swimming zoospores and other developmental stages of
P. palmivora cells. For high magnification microscopic studies, GFP is better visualised and was superior to GUS. In contrast, for macroscopic examination, GUS was superior. Our findings indicate that both GFP and GUS can be used successfully as reporter genes in
P. palmivora.</description><subject>Algal Proteins - genetics</subject><subject>Algal Proteins - metabolism</subject><subject>Animals</subject><subject>b-Glucuronidase</subject><subject>Biological and medical sciences</subject><subject>calcium chloride</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>Generalities. Techniques</subject><subject>Genes, Reporter</subject><subject>genetic transformation</subject><subject>Glucuronidase - genetics</subject><subject>Glucuronidase - metabolism</subject><subject>Green fluorescent protein</subject><subject>Green Fluorescent Proteins</subject><subject>GUS protein</subject><subject>Life Cycle Stages</subject><subject>Luminescent Proteins - genetics</subject><subject>Luminescent Proteins - metabolism</subject><subject>Microbiology</subject><subject>Microscopy, Confocal</subject><subject>Microscopy, Fluorescence</subject><subject>Mycological methods and techniques used in mycology</subject><subject>Mycology</subject><subject>Oomycete</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Phytophthora - chemistry</subject><subject>Phytophthora - enzymology</subject><subject>Phytophthora - genetics</subject><subject>Phytophthora - growth & development</subject><subject>Phytophthora palmivora</subject><subject>plant pathogenic fungi</subject><subject>Plants - microbiology</subject><subject>polyethylene glycol</subject><subject>Scyphozoa</subject><subject>Transformation, Genetic</subject><subject>β-Glucuronidase</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0ctu1DAUBmALgehQeATAC4TaRcCX2I5XCFV0QKpEpdK15TgnHUMSB9tTad4epxkBu65s2Z8v5z8IvabkAyVUfrwhXDUVJVqdaX1OCGekok_QhgpVV1LL5ina_CUn6EVKPwkhNSPyOTqhpG64YmKDfm0jwIT7YR8iJAdTxnMMGfyEz7aX1-fYJmxxhDnEDBHfwQS4DxHnHeB5sAu3eRfKunc4hPHgIAO-3h1ymHdlI9oChtHfl9lL9Ky3Q4JXx_EU3V5--XHxtbr6vv128fmqckLyXGltZetaS4iGthO9s7KXssyEla4hdddSa4XueVFCUC1r5Rg4zqhaYMNP0fv13lLJ7z2kbEZfShvKdyHsk1FLWg3jj0KqSoC0FgWKFboYUorQmzn60caDocQs7TAP7TBL1kZr89AOQ8u5N8cH9u0I3X-n1vwLeHcENjk79NFOzqd_TlPG-MLerqy3wdi7WMjtDSOUE6Z5rdRSyqdVQAn23kM0yXmYHHQ-gsumC_6Rv_4B1PGwyg</recordid><startdate>19990901</startdate><enddate>19990901</enddate><creator>van West, Pieter</creator><creator>Reid, Brian</creator><creator>Campbell, Tracey A.</creator><creator>Sandrock, Robert W.</creator><creator>Fry, William E.</creator><creator>Kamoun, Sophien</creator><creator>Gow, Neil A.R.</creator><general>Elsevier B.V</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19990901</creationdate><title>Green fluorescent protein (GFP) as a reporter gene for the plant pathogenic oomycete Phytophthora palmivora</title><author>van West, Pieter ; Reid, Brian ; Campbell, Tracey A. ; Sandrock, Robert W. ; Fry, William E. ; Kamoun, Sophien ; Gow, Neil A.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c563t-99a6bcba009ebd5fca6f66bd55a6c804db1aa59f36bc5519647c2ec32176f6683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Algal Proteins - genetics</topic><topic>Algal Proteins - metabolism</topic><topic>Animals</topic><topic>b-Glucuronidase</topic><topic>Biological and medical sciences</topic><topic>calcium chloride</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>Generalities. Techniques</topic><topic>Genes, Reporter</topic><topic>genetic transformation</topic><topic>Glucuronidase - genetics</topic><topic>Glucuronidase - metabolism</topic><topic>Green fluorescent protein</topic><topic>Green Fluorescent Proteins</topic><topic>GUS protein</topic><topic>Life Cycle Stages</topic><topic>Luminescent Proteins - genetics</topic><topic>Luminescent Proteins - metabolism</topic><topic>Microbiology</topic><topic>Microscopy, Confocal</topic><topic>Microscopy, Fluorescence</topic><topic>Mycological methods and techniques used in mycology</topic><topic>Mycology</topic><topic>Oomycete</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Phytophthora - chemistry</topic><topic>Phytophthora - enzymology</topic><topic>Phytophthora - genetics</topic><topic>Phytophthora - growth & development</topic><topic>Phytophthora palmivora</topic><topic>plant pathogenic fungi</topic><topic>Plants - microbiology</topic><topic>polyethylene glycol</topic><topic>Scyphozoa</topic><topic>Transformation, Genetic</topic><topic>β-Glucuronidase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van West, Pieter</creatorcontrib><creatorcontrib>Reid, Brian</creatorcontrib><creatorcontrib>Campbell, Tracey A.</creatorcontrib><creatorcontrib>Sandrock, Robert W.</creatorcontrib><creatorcontrib>Fry, William E.</creatorcontrib><creatorcontrib>Kamoun, Sophien</creatorcontrib><creatorcontrib>Gow, Neil A.R.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van West, Pieter</au><au>Reid, Brian</au><au>Campbell, Tracey A.</au><au>Sandrock, Robert W.</au><au>Fry, William E.</au><au>Kamoun, Sophien</au><au>Gow, Neil A.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Green fluorescent protein (GFP) as a reporter gene for the plant pathogenic oomycete Phytophthora palmivora</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>1999-09-01</date><risdate>1999</risdate><volume>178</volume><issue>1</issue><spage>71</spage><epage>80</epage><pages>71-80</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Transgenic
Phytophthora palmivora strains that produce green fluorescent protein (GFP) or β-glucuronidase (GUS) constitutively were obtained after stable DNA integration using a polyethylene-glycol and CaCl
2-based transformation protocol. GFP and GUS production were monitored during several stages of the life cycle of
P. palmivora to evaluate their use in molecular and physiological studies. 40% of the GFP transformants produced the GFP to a level detectable by a confocal laser scanning microscope, whereas 75% of the GUS transformants produced GUS. GFP could be visualised readily in swimming zoospores and other developmental stages of
P. palmivora cells. For high magnification microscopic studies, GFP is better visualised and was superior to GUS. In contrast, for macroscopic examination, GUS was superior. Our findings indicate that both GFP and GUS can be used successfully as reporter genes in
P. palmivora.</abstract><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>10483725</pmid><doi>10.1016/S0378-1097(99)00320-1</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1999-09, Vol.178 (1), p.71-80 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70032823 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Algal Proteins - genetics Algal Proteins - metabolism Animals b-Glucuronidase Biological and medical sciences calcium chloride Fundamental and applied biological sciences. Psychology Fungal plant pathogens Generalities. Techniques Genes, Reporter genetic transformation Glucuronidase - genetics Glucuronidase - metabolism Green fluorescent protein Green Fluorescent Proteins GUS protein Life Cycle Stages Luminescent Proteins - genetics Luminescent Proteins - metabolism Microbiology Microscopy, Confocal Microscopy, Fluorescence Mycological methods and techniques used in mycology Mycology Oomycete Phytopathology. Animal pests. Plant and forest protection Phytophthora - chemistry Phytophthora - enzymology Phytophthora - genetics Phytophthora - growth & development Phytophthora palmivora plant pathogenic fungi Plants - microbiology polyethylene glycol Scyphozoa Transformation, Genetic β-Glucuronidase |
| title | Green fluorescent protein (GFP) as a reporter gene for the plant pathogenic oomycete Phytophthora palmivora |
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