Genome analysis of several marine, magnetotactic bacterial strains by pulsed-field gel electrophoresis
The genomic DNA of three strains of marine magnetotactic bacteria, including two facultatively anaerobic vibrios, strains MV-1 and MV-2, and the microaerophilic coccus, strain MC-1, was analyzed by pulsed-field gel electrophoresis (PFGE). Digestion of the genomic DNA of strain MV-1 by the restrictio...
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| Veröffentlicht in: | Current microbiology 1999-10, Vol.39 (4), p.219-225 |
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| description | The genomic DNA of three strains of marine magnetotactic bacteria, including two facultatively anaerobic vibrios, strains MV-1 and MV-2, and the microaerophilic coccus, strain MC-1, was analyzed by pulsed-field gel electrophoresis (PFGE). Digestion of the genomic DNA of strain MV-1 by the restriction endonucleases AvrII, BamHI, HindIII, NheI, SalI, SfiI, SgfI, SgrAI, and XbaI resulted in a large number of fragments below 400 kb that were difficult to resolve by PFGE. Digestion of MV-1 DNA with NotI and RsrII resulted in no fragments. Treatment of genomic DNA of strains MV-1 and MV-2 with PacI, PmeI, and SpeI yielded a manageable number of fragments (ca. 20) that were relatively easily resolved with PFGE, while PacI and SpeI were effective for strain MC-1. There was no evidence for the presence of plasmids and linear chromosomes in any of the strains, and strains MV-1 and MV-2 appear to contain a single, circular chromosome. Genome sizes of strains MV-1, MV-2, and MC-1 were estimated to be between 3.6 and 3.9 Mb (mean +/- SD; 3.7 +/- 0.2), 3. 3 and 3.7 Mb (3.6 +/- 0.2), and 4.3 and 4.7 Mb (4.5 +/- 0.3), respectively. The restriction fragment patterns of the vibrioid strains MV-1 and MV-2 were extremely similar, suggesting that the strains are closely related. |
| doi_str_mv | 10.1007/s002849900448 |
| format | Article |
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J ; BAZYLINSKI, D. A</creator><creatorcontrib>DEAN, A. J ; BAZYLINSKI, D. A</creatorcontrib><description>The genomic DNA of three strains of marine magnetotactic bacteria, including two facultatively anaerobic vibrios, strains MV-1 and MV-2, and the microaerophilic coccus, strain MC-1, was analyzed by pulsed-field gel electrophoresis (PFGE). Digestion of the genomic DNA of strain MV-1 by the restriction endonucleases AvrII, BamHI, HindIII, NheI, SalI, SfiI, SgfI, SgrAI, and XbaI resulted in a large number of fragments below 400 kb that were difficult to resolve by PFGE. Digestion of MV-1 DNA with NotI and RsrII resulted in no fragments. Treatment of genomic DNA of strains MV-1 and MV-2 with PacI, PmeI, and SpeI yielded a manageable number of fragments (ca. 20) that were relatively easily resolved with PFGE, while PacI and SpeI were effective for strain MC-1. There was no evidence for the presence of plasmids and linear chromosomes in any of the strains, and strains MV-1 and MV-2 appear to contain a single, circular chromosome. Genome sizes of strains MV-1, MV-2, and MC-1 were estimated to be between 3.6 and 3.9 Mb (mean +/- SD; 3.7 +/- 0.2), 3. 3 and 3.7 Mb (3.6 +/- 0.2), and 4.3 and 4.7 Mb (4.5 +/- 0.3), respectively. The restriction fragment patterns of the vibrioid strains MV-1 and MV-2 were extremely similar, suggesting that the strains are closely related.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s002849900448</identifier><identifier>PMID: 10486058</identifier><identifier>CODEN: CUMIDD</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Animal, plant and microbial ecology ; Bacteriology ; Biological and medical sciences ; Chromosomes ; Deoxyribonucleic acid ; DNA ; DNA Restriction Enzymes - metabolism ; Electrophoresis ; Electrophoresis, Gel, Pulsed-Field ; Fundamental and applied biological sciences. 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J</creatorcontrib><creatorcontrib>BAZYLINSKI, D. A</creatorcontrib><title>Genome analysis of several marine, magnetotactic bacterial strains by pulsed-field gel electrophoresis</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><description>The genomic DNA of three strains of marine magnetotactic bacteria, including two facultatively anaerobic vibrios, strains MV-1 and MV-2, and the microaerophilic coccus, strain MC-1, was analyzed by pulsed-field gel electrophoresis (PFGE). Digestion of the genomic DNA of strain MV-1 by the restriction endonucleases AvrII, BamHI, HindIII, NheI, SalI, SfiI, SgfI, SgrAI, and XbaI resulted in a large number of fragments below 400 kb that were difficult to resolve by PFGE. Digestion of MV-1 DNA with NotI and RsrII resulted in no fragments. Treatment of genomic DNA of strains MV-1 and MV-2 with PacI, PmeI, and SpeI yielded a manageable number of fragments (ca. 20) that were relatively easily resolved with PFGE, while PacI and SpeI were effective for strain MC-1. There was no evidence for the presence of plasmids and linear chromosomes in any of the strains, and strains MV-1 and MV-2 appear to contain a single, circular chromosome. Genome sizes of strains MV-1, MV-2, and MC-1 were estimated to be between 3.6 and 3.9 Mb (mean +/- SD; 3.7 +/- 0.2), 3. 3 and 3.7 Mb (3.6 +/- 0.2), and 4.3 and 4.7 Mb (4.5 +/- 0.3), respectively. 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Psychology</topic><topic>Genetics</topic><topic>Genome, Bacterial</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Gram-Negative Bacteria - genetics</topic><topic>Gram-Negative Bacteria - growth & development</topic><topic>Magnetics</topic><topic>Microbial ecology</topic><topic>Microbiology</topic><topic>Various environments (extraatmospheric space, air, water)</topic><topic>Water Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DEAN, A. J</creatorcontrib><creatorcontrib>BAZYLINSKI, D. 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J</au><au>BAZYLINSKI, D. A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome analysis of several marine, magnetotactic bacterial strains by pulsed-field gel electrophoresis</atitle><jtitle>Current microbiology</jtitle><addtitle>Curr Microbiol</addtitle><date>1999-10-01</date><risdate>1999</risdate><volume>39</volume><issue>4</issue><spage>219</spage><epage>225</epage><pages>219-225</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><coden>CUMIDD</coden><abstract>The genomic DNA of three strains of marine magnetotactic bacteria, including two facultatively anaerobic vibrios, strains MV-1 and MV-2, and the microaerophilic coccus, strain MC-1, was analyzed by pulsed-field gel electrophoresis (PFGE). Digestion of the genomic DNA of strain MV-1 by the restriction endonucleases AvrII, BamHI, HindIII, NheI, SalI, SfiI, SgfI, SgrAI, and XbaI resulted in a large number of fragments below 400 kb that were difficult to resolve by PFGE. Digestion of MV-1 DNA with NotI and RsrII resulted in no fragments. Treatment of genomic DNA of strains MV-1 and MV-2 with PacI, PmeI, and SpeI yielded a manageable number of fragments (ca. 20) that were relatively easily resolved with PFGE, while PacI and SpeI were effective for strain MC-1. There was no evidence for the presence of plasmids and linear chromosomes in any of the strains, and strains MV-1 and MV-2 appear to contain a single, circular chromosome. Genome sizes of strains MV-1, MV-2, and MC-1 were estimated to be between 3.6 and 3.9 Mb (mean +/- SD; 3.7 +/- 0.2), 3. 3 and 3.7 Mb (3.6 +/- 0.2), and 4.3 and 4.7 Mb (4.5 +/- 0.3), respectively. The restriction fragment patterns of the vibrioid strains MV-1 and MV-2 were extremely similar, suggesting that the strains are closely related.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>10486058</pmid><doi>10.1007/s002849900448</doi><tpages>7</tpages></addata></record> |
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| subjects | Animal, plant and microbial ecology Bacteriology Biological and medical sciences Chromosomes Deoxyribonucleic acid DNA DNA Restriction Enzymes - metabolism Electrophoresis Electrophoresis, Gel, Pulsed-Field Fundamental and applied biological sciences. Psychology Genetics Genome, Bacterial Genomes Genomics Gram-Negative Bacteria - genetics Gram-Negative Bacteria - growth & development Magnetics Microbial ecology Microbiology Various environments (extraatmospheric space, air, water) Water Microbiology |
| title | Genome analysis of several marine, magnetotactic bacterial strains by pulsed-field gel electrophoresis |
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