Quantitative Detection of Hepatitis B Virus DNA with a New PCR Assay
The Amplicor™ HBV Monitor Test for quantitative detection of serum hepatitis B virus (HBV) DNA has recently been introduced. This assay is based on PCR and a non-radioactive hybridization and detection system on microwell plates. Evaluation in a routine diagnostic laboratory showed excellent sensiti...
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| Veröffentlicht in: | Clinical chemistry and laboratory medicine 1998-08, Vol.36 (8), p.601-604 |
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| container_title | Clinical chemistry and laboratory medicine |
| container_volume | 36 |
| creator | Kessler, Harald H. Pierer, Karen Santner, Brigitte I. Vellimedu, Srinivas K. Stelzl, Evelyn Lackner, Herwig Moser, Andrea Marth, Egon |
| description | The Amplicor™ HBV Monitor Test for quantitative detection of serum hepatitis B virus (HBV) DNA has recently been introduced. This assay is based on PCR and a non-radioactive hybridization and detection system on microwell plates. Evaluation in a routine diagnostic laboratory showed excellent sensitivity and adequate reproducibility; however, a more automated format would be desirable. The Amplicor™ HBV Monitor Test is useful for recognizing those patients who might benefit from antiviral treatment and for evaluation of the efficacy of anti-hepatitis B virus treatment. |
| doi_str_mv | 10.1515/CCLM.1998.105 |
| format | Article |
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This assay is based on PCR and a non-radioactive hybridization and detection system on microwell plates. Evaluation in a routine diagnostic laboratory showed excellent sensitivity and adequate reproducibility; however, a more automated format would be desirable. The Amplicor™ HBV Monitor Test is useful for recognizing those patients who might benefit from antiviral treatment and for evaluation of the efficacy of anti-hepatitis B virus treatment.</description><subject>Biological and medical sciences</subject><subject>DNA, Viral - blood</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hepatitis B virus</subject><subject>Hepatitis B virus - genetics</subject><subject>Hepatitis B virus - isolation & purification</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Techniques used in virology</subject><subject>Viral diseases</subject><subject>Viral hepatitis</subject><subject>Virology</subject><issn>1434-6621</issn><issn>1437-4331</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOwzAQRS0E4lFYskTyArFL8djxa1lSoEjlKUDsLCdxhKFtSuxQ-HtSqGDJakZzj-5IB6F9IH3gwI-zbHzZB61VHwhfQ9uQMpmkjMH6954mQlDYQjshvBACnKdyE21qRUQq9DYa3rZ2Fn200b87PHTRFdHXM1xXeOTm3TX6gE_wo2_agIdXA7zw8RlbfOUW-Ca7w4MQ7Ocu2qjsJLi91eyhh7PT-2yUjK_PL7LBOCmYEjEpJYBUFReMMgmOAReUOGZzThRlkFNdCGWFZsRKDjSnZaVEqYRwusy106yHjn5650391roQzdSHwk0mdubqNhhJCFVawL8gJYqpzlMHJj9g0dQhNK4y88ZPbfNpgJilXrPUa5Z6uwvv-INVcZtPXflLr3x2-eEqt6Gwk6qxs8KHv1IBSkr699aH6D5-Y9u8GiGZ5Ob2PjUZBX03fDoz5-wL5HyNbA</recordid><startdate>19980801</startdate><enddate>19980801</enddate><creator>Kessler, Harald H.</creator><creator>Pierer, Karen</creator><creator>Santner, Brigitte I.</creator><creator>Vellimedu, Srinivas K.</creator><creator>Stelzl, Evelyn</creator><creator>Lackner, Herwig</creator><creator>Moser, Andrea</creator><creator>Marth, Egon</creator><general>Walter de Gruyter</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19980801</creationdate><title>Quantitative Detection of Hepatitis B Virus DNA with a New PCR Assay</title><author>Kessler, Harald H. ; Pierer, Karen ; Santner, Brigitte I. ; Vellimedu, Srinivas K. ; Stelzl, Evelyn ; Lackner, Herwig ; Moser, Andrea ; Marth, Egon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-d71178f5632371e315620e3ab508231b29c68a6930a7512b2df86d866e9db9e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Biological and medical sciences</topic><topic>DNA, Viral - blood</topic><topic>Fundamental and applied biological sciences. 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| fulltext | fulltext |
| identifier | ISSN: 1434-6621 |
| ispartof | Clinical chemistry and laboratory medicine, 1998-08, Vol.36 (8), p.601-604 |
| issn | 1434-6621 1437-4331 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70028961 |
| source | MEDLINE; De Gruyter journals |
| subjects | Biological and medical sciences DNA, Viral - blood Fundamental and applied biological sciences. Psychology Hepatitis B virus Hepatitis B virus - genetics Hepatitis B virus - isolation & purification Human viral diseases Humans Infectious diseases Medical sciences Microbiology Polymerase Chain Reaction - methods Reproducibility of Results Sensitivity and Specificity Techniques used in virology Viral diseases Viral hepatitis Virology |
| title | Quantitative Detection of Hepatitis B Virus DNA with a New PCR Assay |
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