Molecular dissection by gene targeting in the chicken B cell line DT40
The gene-targeting experiment is a powerful tool for clarifying the functions of each gene product. For instance, establishment of a gene-knockout mouse, undoubtedly, contributes to our understanding of the functions of each gene in vivo. However, assuming that the gene product is expressed in vario...
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| Veröffentlicht in: | Folia Pharmacologica Japonica 1999, Vol.113(5), pp.309-316 |
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| container_title | Folia Pharmacologica Japonica |
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| creator | OKADA, Hidetaka KUROSAKI, Tomohiro |
| description | The gene-targeting experiment is a powerful tool for clarifying the functions of each gene product. For instance, establishment of a gene-knockout mouse, undoubtedly, contributes to our understanding of the functions of each gene in vivo. However, assuming that the gene product is expressed in various lineage cells, the phenotype in this knockout mouse exhibits a mixture of defects in various lineage cells. Thus, to compensate for this weak point, cell-based gene-knock experiments are required. Since the DT40 chicken B cell line shows a high frequency of homologous recombination, this cell line can be used for rapid dissection of each gene product at the cellular level. |
| doi_str_mv | 10.1254/fpj.113.309 |
| format | Article |
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Since the DT40 chicken B cell line shows a high frequency of homologous recombination, this cell line can be used for rapid dissection of each gene product at the cellular level.</description><subject>Animals</subject><subject>B-Lymphocytes</subject><subject>BCR</subject><subject>Cells, Cultured</subject><subject>Chickens</subject><subject>conditional mutant</subject><subject>DT40 cells</subject><subject>gene targeting</subject><subject>Gene Targeting - methods</subject><subject>Mutation</subject><subject>SHIP</subject><issn>0015-5691</issn><issn>1347-8397</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkD1PwzAQQC0EggqY2JEnFpTiix1_jFDKhwRigdlKnEtrSJNiJwP_HpdWFcvdcE9Pp0fIBbAp5IW4adafUwA-5cwckAlwoTLNjTokE8agyApp4IScx-grxgqVK8nhmJwAEzqd9YQ8vPYturEtA619jOgG33e0-qEL7JAOZVjg4LsF9R0dlkjd0rsv7Ogdddi2tPUJun8X7IwcNWUb8Xy3T8nHw_x99pS9vD0-z25fMsdBmKyWjTFcSRBaCsZrVecNE1VRK1aB1hJMrpUG6YxGUbNGGeAOGwl5el8UyE_J1da7Dv33iHGwKx83r5Qd9mO0irFc8sIk8HoLutDHGLCx6-BXZfixwOymnE3lbCpnU7lEX-60Y7XC-h-77ZSA-Rb4jEO5wD1QhsG7FjcyMEL8CYvdTOL93S3LYLHjv_n-fmE</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>OKADA, Hidetaka</creator><creator>KUROSAKI, Tomohiro</creator><general>The Japanese Pharmacological Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1999</creationdate><title>Molecular dissection by gene targeting in the chicken B cell line DT40</title><author>OKADA, Hidetaka ; KUROSAKI, Tomohiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3149-d6f993761486403d7d2f04b5d70b188619287816c98e4d0f7913cef612b0045e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>jpn</language><creationdate>1999</creationdate><topic>Animals</topic><topic>B-Lymphocytes</topic><topic>BCR</topic><topic>Cells, Cultured</topic><topic>Chickens</topic><topic>conditional mutant</topic><topic>DT40 cells</topic><topic>gene targeting</topic><topic>Gene Targeting - methods</topic><topic>Mutation</topic><topic>SHIP</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OKADA, Hidetaka</creatorcontrib><creatorcontrib>KUROSAKI, Tomohiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Folia Pharmacologica Japonica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OKADA, Hidetaka</au><au>KUROSAKI, Tomohiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular dissection by gene targeting in the chicken B cell line DT40</atitle><jtitle>Folia Pharmacologica Japonica</jtitle><addtitle>Nihon Yakurigaku Zasshi</addtitle><date>1999</date><risdate>1999</risdate><volume>113</volume><issue>5</issue><spage>309</spage><epage>316</epage><pages>309-316</pages><issn>0015-5691</issn><eissn>1347-8397</eissn><abstract>The gene-targeting experiment is a powerful tool for clarifying the functions of each gene product. 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| ispartof | Folia Pharmacologica Japonica, 1999, Vol.113(5), pp.309-316 |
| issn | 0015-5691 1347-8397 |
| language | jpn |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals B-Lymphocytes BCR Cells, Cultured Chickens conditional mutant DT40 cells gene targeting Gene Targeting - methods Mutation SHIP |
| title | Molecular dissection by gene targeting in the chicken B cell line DT40 |
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