The regulated expression of an intrabody produces a mutant phenotype in Drosophila
Intrabodies show great promise for controlling gene expression. As an initial attempt to evaluate the intrabody technology in Drosophila, the gene poxn was used as target. Transgenic flies harboring different anti-Poxn scFv genes integrated into various chromosomes were obtained. In one transformant...
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| Veröffentlicht in: | FEBS letters 1998-10, Vol.437 (1), p.81-86 |
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| container_title | FEBS letters |
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| creator | Hassanzadeh Gh, Gholamreza Devoogdt, Nick Ghysen, Alain De Baetselier, Patrick Muyldermans, Serge Dambly-Chaudière, Christine |
| description | Intrabodies show great promise for controlling gene expression. As an initial attempt to evaluate the intrabody technology in
Drosophila, the gene
poxn was used as target. Transgenic flies harboring different anti-Poxn scFv genes integrated into various chromosomes were obtained. In one transformant, a phenocopy resembling the hypomorphic
poxn
− phenotype was produced in embryos and larvae following induction of expression of α-Poxn2 intrabody. The antisense approach was used as control. Parameters that can affect the success of intrabody technology are described. |
| doi_str_mv | 10.1016/S0014-5793(98)01205-8 |
| format | Article |
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Drosophila, the gene
poxn was used as target. Transgenic flies harboring different anti-Poxn scFv genes integrated into various chromosomes were obtained. In one transformant, a phenocopy resembling the hypomorphic
poxn
− phenotype was produced in embryos and larvae following induction of expression of α-Poxn2 intrabody. The antisense approach was used as control. Parameters that can affect the success of intrabody technology are described.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(98)01205-8</identifier><identifier>PMID: 9804176</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Animals, Genetically Modified ; Antisense ; CNS, central nervous system ; DNA, Antisense ; Drosophila ; Drosophila - genetics ; Drosophila Proteins ; es organ, external sense organ ; Gene Expression Regulation ; Hot Temperature ; Immunoglobulin Fragments ; Intrabody ; m-es organ, mono-innervated external sense organ ; Mutation ; Nerve Tissue Proteins - genetics ; p-es organ, poly-innervated external sense organ ; Paired Box Transcription Factors ; PCR, polymerase chain reaction ; Phenocopy ; Phenotype ; PNS, peripheral nervous system ; Poxn ; scFv, single-chain variable fragment ; Transcription Factors ; Transfection</subject><ispartof>FEBS letters, 1998-10, Vol.437 (1), p.81-86</ispartof><rights>1998 Federation of European Biochemical Societies</rights><rights>FEBS Letters 437 (1998) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4258-9234d77a760c6205b4ec8dbfc7ee9a8afb95d1dd449591dd395786bf933996fa3</citedby><cites>FETCH-LOGICAL-c4258-9234d77a760c6205b4ec8dbfc7ee9a8afb95d1dd449591dd395786bf933996fa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2898%2901205-8$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014579398012058$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,1427,3537,27901,27902,45550,45551,46384,46808,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9804176$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hassanzadeh Gh, Gholamreza</creatorcontrib><creatorcontrib>Devoogdt, Nick</creatorcontrib><creatorcontrib>Ghysen, Alain</creatorcontrib><creatorcontrib>De Baetselier, Patrick</creatorcontrib><creatorcontrib>Muyldermans, Serge</creatorcontrib><creatorcontrib>Dambly-Chaudière, Christine</creatorcontrib><title>The regulated expression of an intrabody produces a mutant phenotype in Drosophila</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Intrabodies show great promise for controlling gene expression. As an initial attempt to evaluate the intrabody technology in
Drosophila, the gene
poxn was used as target. Transgenic flies harboring different anti-Poxn scFv genes integrated into various chromosomes were obtained. In one transformant, a phenocopy resembling the hypomorphic
poxn
− phenotype was produced in embryos and larvae following induction of expression of α-Poxn2 intrabody. The antisense approach was used as control. Parameters that can affect the success of intrabody technology are described.</description><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Antisense</subject><subject>CNS, central nervous system</subject><subject>DNA, Antisense</subject><subject>Drosophila</subject><subject>Drosophila - genetics</subject><subject>Drosophila Proteins</subject><subject>es organ, external sense organ</subject><subject>Gene Expression Regulation</subject><subject>Hot Temperature</subject><subject>Immunoglobulin Fragments</subject><subject>Intrabody</subject><subject>m-es organ, mono-innervated external sense organ</subject><subject>Mutation</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>p-es organ, poly-innervated external sense organ</subject><subject>Paired Box Transcription Factors</subject><subject>PCR, polymerase chain reaction</subject><subject>Phenocopy</subject><subject>Phenotype</subject><subject>PNS, peripheral nervous system</subject><subject>Poxn</subject><subject>scFv, single-chain variable fragment</subject><subject>Transcription Factors</subject><subject>Transfection</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkFFP2zAUha2JiRW2n1DJTwgestlJHNtPCLp2TEKatLFny7FvVqM0DnbC6L_Haau-wtOVfc499-hDaE7JV0po9e0PIbTMGJfFpRRXhOaEZeIDmlHBi6woK3GCZkfLJ3QW4yNJb0HlKTqVgpSUVzP0-2ENOMC_sdUDWAwvfYAYne-wb7DusOuGoGtvt7gP3o4GItZ4Mw66G3C_hs4P2x6SC38PPvp-7Vr9GX1sdBvhy2Geo7-r5cPiLrv_9ePn4uY-M2XORCbzorSca14RU6XydQlG2LoxHEBqoZtaMkutLUvJZJqFZFxUdSOLQsqq0cU5utjnpmZPI8RBbVw00La6Az9GxQnJWcVYMrK90aSOMUCj-uA2OmwVJWpiqXYs1QRKSaF2LJVIe_PDgbHegD1uHeAl_W6v_3ctbN8XqlbL23ynTEJKmr6nU9f7KEjAnh0EFY2DzoB1AcygrHdvlH0F426YRw</recordid><startdate>19981016</startdate><enddate>19981016</enddate><creator>Hassanzadeh Gh, Gholamreza</creator><creator>Devoogdt, Nick</creator><creator>Ghysen, Alain</creator><creator>De Baetselier, Patrick</creator><creator>Muyldermans, Serge</creator><creator>Dambly-Chaudière, Christine</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19981016</creationdate><title>The regulated expression of an intrabody produces a mutant phenotype in Drosophila</title><author>Hassanzadeh Gh, Gholamreza ; Devoogdt, Nick ; Ghysen, Alain ; De Baetselier, Patrick ; Muyldermans, Serge ; Dambly-Chaudière, Christine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4258-9234d77a760c6205b4ec8dbfc7ee9a8afb95d1dd449591dd395786bf933996fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Animals, Genetically Modified</topic><topic>Antisense</topic><topic>CNS, central nervous system</topic><topic>DNA, Antisense</topic><topic>Drosophila</topic><topic>Drosophila - genetics</topic><topic>Drosophila Proteins</topic><topic>es organ, external sense organ</topic><topic>Gene Expression Regulation</topic><topic>Hot Temperature</topic><topic>Immunoglobulin Fragments</topic><topic>Intrabody</topic><topic>m-es organ, mono-innervated external sense organ</topic><topic>Mutation</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>p-es organ, poly-innervated external sense organ</topic><topic>Paired Box Transcription Factors</topic><topic>PCR, polymerase chain reaction</topic><topic>Phenocopy</topic><topic>Phenotype</topic><topic>PNS, peripheral nervous system</topic><topic>Poxn</topic><topic>scFv, single-chain variable fragment</topic><topic>Transcription Factors</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hassanzadeh Gh, Gholamreza</creatorcontrib><creatorcontrib>Devoogdt, Nick</creatorcontrib><creatorcontrib>Ghysen, Alain</creatorcontrib><creatorcontrib>De Baetselier, Patrick</creatorcontrib><creatorcontrib>Muyldermans, Serge</creatorcontrib><creatorcontrib>Dambly-Chaudière, Christine</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hassanzadeh Gh, Gholamreza</au><au>Devoogdt, Nick</au><au>Ghysen, Alain</au><au>De Baetselier, Patrick</au><au>Muyldermans, Serge</au><au>Dambly-Chaudière, Christine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The regulated expression of an intrabody produces a mutant phenotype in Drosophila</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1998-10-16</date><risdate>1998</risdate><volume>437</volume><issue>1</issue><spage>81</spage><epage>86</epage><pages>81-86</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Intrabodies show great promise for controlling gene expression. As an initial attempt to evaluate the intrabody technology in
Drosophila, the gene
poxn was used as target. Transgenic flies harboring different anti-Poxn scFv genes integrated into various chromosomes were obtained. In one transformant, a phenocopy resembling the hypomorphic
poxn
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| ispartof | FEBS letters, 1998-10, Vol.437 (1), p.81-86 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Animals, Genetically Modified Antisense CNS, central nervous system DNA, Antisense Drosophila Drosophila - genetics Drosophila Proteins es organ, external sense organ Gene Expression Regulation Hot Temperature Immunoglobulin Fragments Intrabody m-es organ, mono-innervated external sense organ Mutation Nerve Tissue Proteins - genetics p-es organ, poly-innervated external sense organ Paired Box Transcription Factors PCR, polymerase chain reaction Phenocopy Phenotype PNS, peripheral nervous system Poxn scFv, single-chain variable fragment Transcription Factors Transfection |
| title | The regulated expression of an intrabody produces a mutant phenotype in Drosophila |
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