Imaging Ca2+ concentration changes at the secretory vesicle surface with a recombinant targeted cameleon

Regulated exocytosis involves the Ca2+-triggered fusion of secretory vesicles with the plasma membrane, by activation of vesicle membrane Ca2+-binding proteins [1]. The Ca2+-binding sites of these proteins are likely to lie within 30 nm of the vesicle surface, a domain in which changes in Ca2+ conce...

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