A Sensitive New Method for Rapid Detection of Abnormal Methylation Patterns in Global DNA and within CpG Islands

To assess alterations in DNA methylation density in both global DNA and within CpG islands, we have developed a simple method based on the use of methylation-sensitive restriction endonucleases that leave a 5′ guanine overhang after DNA cleavage, with subsequent single nucleotide extension with radi...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Biochemical and biophysical research communications 1999-09, Vol.262 (3), p.624-628
Hauptverfasser:	Pogribny, Igor, Yi, Ping, James, S.Jill
Format:	Artikel
Sprache:	eng
Schlagworte:	Carcinoma, Hepatocellular Deoxycytosine Nucleotides - metabolism Dinucleoside Phosphates - analysis DNA - chemistry DNA - genetics DNA Methylation DNA Restriction Enzymes DNA, Neoplasm - chemistry DNA, Neoplasm - metabolism Humans Liver Neoplasms Restriction Mapping - methods Sensitivity and Specificity Tritium Tumor Cells, Cultured
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!