Nuclear targeting peptide scaffolds for lipofection of nondividing mammalian cells

Lipofection of nondividing cells is inefficient because much of the transfected DNA is retained in endosomes, and that which escapes to the cytoplasm enters the nucleus at low rates. To improve the final rate-limiting step of nuclear import, we conjugated a nonclassical nuclear localization signal (...

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Veröffentlicht in:	Nature biotechnology 1999-09, Vol.17 (9), p.873-877
Hauptverfasser:	Diamond, Scott L, Subramanian, Ajit, Ranganathan, P
Format:	Artikel
Sprache:	eng
Schlagworte:	Agriculture Animals Antigens Antigens, Viral, Tumor - genetics Antigens, Viral, Tumor - metabolism Bioinformatics Biological and medical sciences Biological Transport Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Cations Cattle Cell Compartmentation Cell Nucleus - metabolism Conjugates Conserved sequence Cytoplasm Deoxyribonucleic acid DNA Endosomes Endothelium Endothelium, Vascular - cytology Fluorescence Fundamental and applied biological sciences. Psychology Gene expression Gene therapy Gene transfer Health. Pharmaceutical industry Heterogeneous Nuclear Ribonucleoprotein A1 Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins Imports Industrial applications and implications. Economical aspects Life Sciences Lipids Liposomes Localization Mammalian cells Nuclear Localization Signals Nuclear transport Nuclei (cytology) Nucleotide sequence Peptide Fragments - genetics Peptide Fragments - metabolism Protein Sorting Signals - genetics Protein Sorting Signals - metabolism research-article Ribonucleoproteins - genetics Ribonucleoproteins - metabolism Scaffolds Simian virus 40 Transfection Transfection - methods Wheat germ Wheat germ agglutinin
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