Identification of two distinct tumor-suppressor loci on the long arm of chromosome 10 in small cell lung cancer
Recent cytogenetic studies indicated that loss of the long arm of chromosome 10 is a frequent event in small cell lung cancer (SCLC) and that a common region of the deletion is at 10q24-qter, which suggests the presence of a tumor-suppressor gene there. To map precise tumor-suppressor loci on the ch...
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| Veröffentlicht in: | Oncogene 1998-10, Vol.17 (13), p.1749-1753 |
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| creator | KIM, S. K RO, J. Y KEMP, B. L JIN SOO LEE TAE JUNG KWON HONG, W. K LI MAO |
| description | Recent cytogenetic studies indicated that loss of the long arm of chromosome 10 is a frequent event in small cell lung cancer (SCLC) and that a common region of the deletion is at 10q24-qter, which suggests the presence of a tumor-suppressor gene there. To map precise tumor-suppressor loci on the chromosome arm for further positional cloning efforts, we tested 46 primary SCLCs using microsatellite analysis. By analysing 11 highly polymorphic microsatellite markers located in 10q23-q26, we found that at least 78% (36/46) of the tumors exhibited loss of heterozygosity (LOH) at 10q with at least two distinct minimally deleted regions. LOH at one region (10q24) was found in at least 74% (32/43) of informative cases with a minimally deleted region between D10S198 and D1OS192 (about 2 cM); LOH at another region (10q24-q25) was observed in at least 66% (29/44) of informative tumors with a minimally deleted region between D10S221 and D10S587 (about 11 cM). LOH at both regions or across both regions was observed in at least 52% (24/46) of the tumors tested. However, no mutations or homozygous deletions were found in the coding region of MXI1, a candidate tumor suppressor gene at 10q24-q25, in a panel of SCLC cell lines. Our data demonstrate that at least two tumor-suppressor loci exist on 10q and that they may play an important role in SCLC tumorigenesis. |
| doi_str_mv | 10.1038/sj.onc.1202073 |
| format | Article |
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K ; RO, J. Y ; KEMP, B. L ; JIN SOO LEE ; TAE JUNG KWON ; HONG, W. K ; LI MAO</creator><creatorcontrib>KIM, S. K ; RO, J. Y ; KEMP, B. L ; JIN SOO LEE ; TAE JUNG KWON ; HONG, W. K ; LI MAO</creatorcontrib><description>Recent cytogenetic studies indicated that loss of the long arm of chromosome 10 is a frequent event in small cell lung cancer (SCLC) and that a common region of the deletion is at 10q24-qter, which suggests the presence of a tumor-suppressor gene there. To map precise tumor-suppressor loci on the chromosome arm for further positional cloning efforts, we tested 46 primary SCLCs using microsatellite analysis. By analysing 11 highly polymorphic microsatellite markers located in 10q23-q26, we found that at least 78% (36/46) of the tumors exhibited loss of heterozygosity (LOH) at 10q with at least two distinct minimally deleted regions. LOH at one region (10q24) was found in at least 74% (32/43) of informative cases with a minimally deleted region between D10S198 and D1OS192 (about 2 cM); LOH at another region (10q24-q25) was observed in at least 66% (29/44) of informative tumors with a minimally deleted region between D10S221 and D10S587 (about 11 cM). LOH at both regions or across both regions was observed in at least 52% (24/46) of the tumors tested. However, no mutations or homozygous deletions were found in the coding region of MXI1, a candidate tumor suppressor gene at 10q24-q25, in a panel of SCLC cell lines. Our data demonstrate that at least two tumor-suppressor loci exist on 10q and that they may play an important role in SCLC tumorigenesis.</description><identifier>ISSN: 0950-9232</identifier><identifier>EISSN: 1476-5594</identifier><identifier>DOI: 10.1038/sj.onc.1202073</identifier><identifier>PMID: 9796705</identifier><language>eng</language><publisher>Basingstoke: Nature Publishing</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Basic Helix-Loop-Helix Transcription Factors ; Biological and medical sciences ; Carcinoma, Small Cell - genetics ; Cell physiology ; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes ; Chromosome 10 ; Chromosomes ; Chromosomes, Human, Pair 10 ; Cytogenetics ; DNA-Binding Proteins - genetics ; Female ; Fundamental and applied biological sciences. 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By analysing 11 highly polymorphic microsatellite markers located in 10q23-q26, we found that at least 78% (36/46) of the tumors exhibited loss of heterozygosity (LOH) at 10q with at least two distinct minimally deleted regions. LOH at one region (10q24) was found in at least 74% (32/43) of informative cases with a minimally deleted region between D10S198 and D1OS192 (about 2 cM); LOH at another region (10q24-q25) was observed in at least 66% (29/44) of informative tumors with a minimally deleted region between D10S221 and D10S587 (about 11 cM). LOH at both regions or across both regions was observed in at least 52% (24/46) of the tumors tested. However, no mutations or homozygous deletions were found in the coding region of MXI1, a candidate tumor suppressor gene at 10q24-q25, in a panel of SCLC cell lines. 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Psychology</subject><subject>Gene deletion</subject><subject>Genes, Tumor Suppressor</subject><subject>Genetic markers</subject><subject>Heterozygosity</subject><subject>Humans</subject><subject>Loss of Heterozygosity</subject><subject>Lung cancer</subject><subject>Lung Neoplasms - genetics</subject><subject>Male</subject><subject>Microsatellites</subject><subject>Middle Aged</subject><subject>Molecular and cellular biology</subject><subject>Small cell lung carcinoma</subject><subject>Transcription Factors - genetics</subject><subject>Tumor suppressor genes</subject><subject>Tumor Suppressor Proteins</subject><subject>Tumorigenesis</subject><subject>Tumors</subject><issn>0950-9232</issn><issn>1476-5594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo67h69SYEFG89VuWj0znK4sfCghc9h3Q67WboTsYkjfjvzbDNHrx4SQXep4qkHkJeIxwR-PChnI4puiMyYKD4E3JAofpOSi2ekgNoCZ1mnD0nL0o5AYDSwK7IlVa6VyAPJN1OPtYwB2drSJGmmdbfiU6h1BBdpXVbU-7Kdj5nX0rKdEku0AbWe9_u8Se1eb10ufuc1lTS6ikCDZGW1S4Ldb4dy9Y4Z6Pz-SV5Ntul-Fd7vSY_Pn_6fvO1u_v25fbm413nxCBqp3EUI3d6dEwJi3YUArmTKKTWcoRR-8GLXk0WJeDgkfvesgnEMNrBT_3Mr8n7h7nnnH5tvlSzhnJ5jI0-bcUoAGQc8L8gKtAcxQV8-w94SluO7ROG9S0HBdg36vhAuZxKyX425xxWm_8YBHMRZsrJNGFmF9Ya3uxjt3H10yO-G2r5uz23xdllzm2NoTxiTHCJzfBfe36eRQ</recordid><startdate>19981001</startdate><enddate>19981001</enddate><creator>KIM, S. 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K ; LI MAO</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-91b4b3c9bc274a1ab4413c5145995b0b9e8e467da15018e13e6a2d048ba8ed6f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Basic Helix-Loop-Helix Transcription Factors</topic><topic>Biological and medical sciences</topic><topic>Carcinoma, Small Cell - genetics</topic><topic>Cell physiology</topic><topic>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</topic><topic>Chromosome 10</topic><topic>Chromosomes</topic><topic>Chromosomes, Human, Pair 10</topic><topic>Cytogenetics</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene deletion</topic><topic>Genes, Tumor Suppressor</topic><topic>Genetic markers</topic><topic>Heterozygosity</topic><topic>Humans</topic><topic>Loss of Heterozygosity</topic><topic>Lung cancer</topic><topic>Lung Neoplasms - genetics</topic><topic>Male</topic><topic>Microsatellites</topic><topic>Middle Aged</topic><topic>Molecular and cellular biology</topic><topic>Small cell lung carcinoma</topic><topic>Transcription Factors - genetics</topic><topic>Tumor suppressor genes</topic><topic>Tumor Suppressor Proteins</topic><topic>Tumorigenesis</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KIM, S. K</creatorcontrib><creatorcontrib>RO, J. Y</creatorcontrib><creatorcontrib>KEMP, B. L</creatorcontrib><creatorcontrib>JIN SOO LEE</creatorcontrib><creatorcontrib>TAE JUNG KWON</creatorcontrib><creatorcontrib>HONG, W. 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K</au><au>RO, J. Y</au><au>KEMP, B. L</au><au>JIN SOO LEE</au><au>TAE JUNG KWON</au><au>HONG, W. K</au><au>LI MAO</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of two distinct tumor-suppressor loci on the long arm of chromosome 10 in small cell lung cancer</atitle><jtitle>Oncogene</jtitle><addtitle>Oncogene</addtitle><date>1998-10-01</date><risdate>1998</risdate><volume>17</volume><issue>13</issue><spage>1749</spage><epage>1753</epage><pages>1749-1753</pages><issn>0950-9232</issn><eissn>1476-5594</eissn><abstract>Recent cytogenetic studies indicated that loss of the long arm of chromosome 10 is a frequent event in small cell lung cancer (SCLC) and that a common region of the deletion is at 10q24-qter, which suggests the presence of a tumor-suppressor gene there. To map precise tumor-suppressor loci on the chromosome arm for further positional cloning efforts, we tested 46 primary SCLCs using microsatellite analysis. By analysing 11 highly polymorphic microsatellite markers located in 10q23-q26, we found that at least 78% (36/46) of the tumors exhibited loss of heterozygosity (LOH) at 10q with at least two distinct minimally deleted regions. LOH at one region (10q24) was found in at least 74% (32/43) of informative cases with a minimally deleted region between D10S198 and D1OS192 (about 2 cM); LOH at another region (10q24-q25) was observed in at least 66% (29/44) of informative tumors with a minimally deleted region between D10S221 and D10S587 (about 11 cM). LOH at both regions or across both regions was observed in at least 52% (24/46) of the tumors tested. However, no mutations or homozygous deletions were found in the coding region of MXI1, a candidate tumor suppressor gene at 10q24-q25, in a panel of SCLC cell lines. 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| source | MEDLINE; Nature; EZB-FREE-00999 freely available EZB journals; SpringerLink Journals - AutoHoldings |
| subjects | Adult Aged Aged, 80 and over Basic Helix-Loop-Helix Transcription Factors Biological and medical sciences Carcinoma, Small Cell - genetics Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Chromosome 10 Chromosomes Chromosomes, Human, Pair 10 Cytogenetics DNA-Binding Proteins - genetics Female Fundamental and applied biological sciences. Psychology Gene deletion Genes, Tumor Suppressor Genetic markers Heterozygosity Humans Loss of Heterozygosity Lung cancer Lung Neoplasms - genetics Male Microsatellites Middle Aged Molecular and cellular biology Small cell lung carcinoma Transcription Factors - genetics Tumor suppressor genes Tumor Suppressor Proteins Tumorigenesis Tumors |
| title | Identification of two distinct tumor-suppressor loci on the long arm of chromosome 10 in small cell lung cancer |
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