Functional Conservations of the Alkaline Nuclease of Herpes Simplex Type 1 and Human Cytomegalovirus
The herpes simplex virus type 1 UL12 gene product, alkaline nuclease (AN), appears to be involved in viral DNA processing and capsid egress from the nucleus (Shao, L., Rapp, L. M., and Weller, S. K.,Virology196, 146–162, 1993). Although the HSV-1 AN is not absolutely essential for viral replication...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1998-09, Vol.249 (2), p.460-470 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Gao, Min Robertson, Barbara J. McCann, Patrick J. O'Boyle, Donald R. Weller, Sandra K. Newcomb, William W. Brown, Jay C. Weinheimer, Steven P. |
| description | The herpes simplex virus type 1 UL12 gene product, alkaline nuclease (AN), appears to be involved in viral DNA processing and capsid egress from the nucleus (Shao, L., Rapp, L. M., and Weller, S. K.,Virology196, 146–162, 1993). Although the HSV-1 AN is not absolutely essential for viral replication in tissue culture, conservation of the AN gene in all herpesviruses suggests an important role in the life cycle of herpesviruses. The counterpart of HSV-1 AN for human cytomegalovirus (HCMV) is the UL98 gene product. To examine whether the HCMV AN could substitute for HSV-1 AN, we performedtrans-complementation experiments using a HSV-1 amplicon plasmid carrying the HCMV UL98 gene. Our results indicate (i) HCMV AN can complement the growth of the HSV-1 AN deletion mutantUL12lacZvirus intrans;(ii) a new recombinant virus,UL12laZcUL98/99,appears to be generated by the integration of the HCMV UL98 gene into the HSV-1UL12lacZviral genome; (iii) in contrast to its parental HSV-1UL12lacZvirus, capsids formed inUL12lacZUL98/99-infected Vero cells were able to transport from the nucleus to the cytoplasm and mature into infectious viruses. Our results demonstrate a functional conservation of AN between HSV-1 and HCMV. |
| doi_str_mv | 10.1006/viro.1998.9344 |
| format | Article |
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M., and Weller, S. K.,Virology196, 146–162, 1993). Although the HSV-1 AN is not absolutely essential for viral replication in tissue culture, conservation of the AN gene in all herpesviruses suggests an important role in the life cycle of herpesviruses. The counterpart of HSV-1 AN for human cytomegalovirus (HCMV) is the UL98 gene product. To examine whether the HCMV AN could substitute for HSV-1 AN, we performedtrans-complementation experiments using a HSV-1 amplicon plasmid carrying the HCMV UL98 gene. Our results indicate (i) HCMV AN can complement the growth of the HSV-1 AN deletion mutantUL12lacZvirus intrans;(ii) a new recombinant virus,UL12laZcUL98/99,appears to be generated by the integration of the HCMV UL98 gene into the HSV-1UL12lacZviral genome; (iii) in contrast to its parental HSV-1UL12lacZvirus, capsids formed inUL12lacZUL98/99-infected Vero cells were able to transport from the nucleus to the cytoplasm and mature into infectious viruses. Our results demonstrate a functional conservation of AN between HSV-1 and HCMV.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1006/viro.1998.9344</identifier><identifier>PMID: 9791036</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cercopithecus aethiops ; Chromosome Mapping ; Cytomegalovirus - enzymology ; Cytomegalovirus - genetics ; Cytomegalovirus - growth & development ; Evolution, Molecular ; Gene Deletion ; Genes, Viral ; Genetic Complementation Test ; Herpes simplex virus 1 ; Herpesvirus 1, Human - enzymology ; Herpesvirus 1, Human - genetics ; Herpesvirus 1, Human - growth & development ; Human cytomegalovirus ; Humans ; Lac Operon ; Microscopy, Electron ; Mutation ; Ribonucleases - genetics ; Ribonucleases - metabolism ; Species Specificity ; Vero Cells</subject><ispartof>Virology (New York, N.Y.), 1998-09, Vol.249 (2), p.460-470</ispartof><rights>1998 Academic Press</rights><rights>Copyright 1998 Academic Press.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-9748f2c8201cd5cd2a843772e780e6be3ffe2fb0add50ddc841da54618e1964d3</citedby><cites>FETCH-LOGICAL-c410t-9748f2c8201cd5cd2a843772e780e6be3ffe2fb0add50ddc841da54618e1964d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/viro.1998.9344$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9791036$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gao, Min</creatorcontrib><creatorcontrib>Robertson, Barbara J.</creatorcontrib><creatorcontrib>McCann, Patrick J.</creatorcontrib><creatorcontrib>O'Boyle, Donald R.</creatorcontrib><creatorcontrib>Weller, Sandra K.</creatorcontrib><creatorcontrib>Newcomb, William W.</creatorcontrib><creatorcontrib>Brown, Jay C.</creatorcontrib><creatorcontrib>Weinheimer, Steven P.</creatorcontrib><title>Functional Conservations of the Alkaline Nuclease of Herpes Simplex Type 1 and Human Cytomegalovirus</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>The herpes simplex virus type 1 UL12 gene product, alkaline nuclease (AN), appears to be involved in viral DNA processing and capsid egress from the nucleus (Shao, L., Rapp, L. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Cercopithecus aethiops Chromosome Mapping Cytomegalovirus - enzymology Cytomegalovirus - genetics Cytomegalovirus - growth & development Evolution, Molecular Gene Deletion Genes, Viral Genetic Complementation Test Herpes simplex virus 1 Herpesvirus 1, Human - enzymology Herpesvirus 1, Human - genetics Herpesvirus 1, Human - growth & development Human cytomegalovirus Humans Lac Operon Microscopy, Electron Mutation Ribonucleases - genetics Ribonucleases - metabolism Species Specificity Vero Cells |
| title | Functional Conservations of the Alkaline Nuclease of Herpes Simplex Type 1 and Human Cytomegalovirus |
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