Expression of milk protein genes is induced directly by exogenous STAT5 without prolactin-mediated signal transduction in transgenic mice
STAT5 is a member of the family of STAT (signal transducer for activating transcription), which was isolated from nuclear extracts of the lactating gland of sheep. It was reported that, in vitro, STAT5 could be activated directly by prolactin (PRL) without PRL‐mediated signal transduction. The prese...
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| Veröffentlicht in: | Molecular reproduction and development 1999-10, Vol.54 (2), p.121-125 |
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| creator | Inuzuka, H Yamanouchi, K Tachi, C Tojo, H |
| description | STAT5 is a member of the family of STAT (signal transducer for activating transcription), which was isolated from nuclear extracts of the lactating gland of sheep. It was reported that, in vitro, STAT5 could be activated directly by prolactin (PRL) without PRL‐mediated signal transduction. The present study was conducted to investigate above the possibility in vivo, using the transgenic mice overexpressing ovine STAT5 (oSTAT5) under the control of MMTV promoter. Three transgenic mouse lines that expressed the exogenously introduced oSTAT5 in their mammary glands were established. The expression levels of exogenous oSTAT5 were higher than those of endogenous STAT in the mammary glands of all of the three transgenic lines. Although the expression levels of endogenous milk protein genes, WAP, and β‐casein genes, were not correlated with oSTAT5 expression, the expression levels of WAP and β‐casein were induced by exogenous oSTAT5 in the transgenic mice. The present study demonstrated, at very least, that STAT5 expression can directly activate the expression of milk protein genes, and particularly the WAP gene without PRL‐mediated signal transduction. Mol. Reprod. Dev. 54:121–125, 1999. © 1999 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/(SICI)1098-2795(199910)54:2<121::AID-MRD3>3.0.CO;2-T |
| format | Article |
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It was reported that, in vitro, STAT5 could be activated directly by prolactin (PRL) without PRL‐mediated signal transduction. The present study was conducted to investigate above the possibility in vivo, using the transgenic mice overexpressing ovine STAT5 (oSTAT5) under the control of MMTV promoter. Three transgenic mouse lines that expressed the exogenously introduced oSTAT5 in their mammary glands were established. The expression levels of exogenous oSTAT5 were higher than those of endogenous STAT in the mammary glands of all of the three transgenic lines. Although the expression levels of endogenous milk protein genes, WAP, and β‐casein genes, were not correlated with oSTAT5 expression, the expression levels of WAP and β‐casein were induced by exogenous oSTAT5 in the transgenic mice. The present study demonstrated, at very least, that STAT5 expression can directly activate the expression of milk protein genes, and particularly the WAP gene without PRL‐mediated signal transduction. Mol. Reprod. Dev. 54:121–125, 1999. © 1999 Wiley‐Liss, Inc.</description><identifier>ISSN: 1040-452X</identifier><identifier>EISSN: 1098-2795</identifier><identifier>DOI: 10.1002/(SICI)1098-2795(199910)54:2<121::AID-MRD3>3.0.CO;2-T</identifier><identifier>PMID: 10471471</identifier><identifier>CODEN: MREDEE</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Animals ; Artificial Gene Fusion ; Biological and medical sciences ; Breast - metabolism ; Caseins - genetics ; Caseins - metabolism ; DNA-Binding Proteins - pharmacology ; DNA-Binding Proteins - physiology ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression - drug effects ; Gene Expression - physiology ; genes ; Mice ; Mice, Inbred C57BL ; Mice, Inbred ICR ; Mice, Transgenic ; milk proteins ; Milk Proteins - genetics ; Milk Proteins - metabolism ; Models, Genetic ; Molecular and cellular biology ; Molecular genetics ; ovine STAT5 ; prolactin ; Prolactin - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; signal transducer for activating transcription ; signal transduction ; Signal Transduction - physiology ; STAT5 Transcription Factor ; Trans-Activators - pharmacology ; Trans-Activators - physiology ; transgenic animals ; transgenic mice ; WAP ; Whey Proteins ; β-casein</subject><ispartof>Molecular reproduction and development, 1999-10, Vol.54 (2), p.121-125</ispartof><rights>Copyright © 1999 Wiley‐Liss, Inc.</rights><rights>1999 INIST-CNRS</rights><rights>Copyright 1999 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c5183-8a889e4618f93079af562dfdb3adbbae2c12392a7494e1723fbc046b04d06b4d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291098-2795%28199910%2954%3A2%3C121%3A%3AAID-MRD3%3E3.0.CO%3B2-T$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291098-2795%28199910%2954%3A2%3C121%3A%3AAID-MRD3%3E3.0.CO%3B2-T$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1984508$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10471471$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inuzuka, H</creatorcontrib><creatorcontrib>Yamanouchi, K</creatorcontrib><creatorcontrib>Tachi, C</creatorcontrib><creatorcontrib>Tojo, H</creatorcontrib><title>Expression of milk protein genes is induced directly by exogenous STAT5 without prolactin-mediated signal transduction in transgenic mice</title><title>Molecular reproduction and development</title><addtitle>Mol. Reprod. Dev</addtitle><description>STAT5 is a member of the family of STAT (signal transducer for activating transcription), which was isolated from nuclear extracts of the lactating gland of sheep. It was reported that, in vitro, STAT5 could be activated directly by prolactin (PRL) without PRL‐mediated signal transduction. The present study was conducted to investigate above the possibility in vivo, using the transgenic mice overexpressing ovine STAT5 (oSTAT5) under the control of MMTV promoter. Three transgenic mouse lines that expressed the exogenously introduced oSTAT5 in their mammary glands were established. The expression levels of exogenous oSTAT5 were higher than those of endogenous STAT in the mammary glands of all of the three transgenic lines. Although the expression levels of endogenous milk protein genes, WAP, and β‐casein genes, were not correlated with oSTAT5 expression, the expression levels of WAP and β‐casein were induced by exogenous oSTAT5 in the transgenic mice. The present study demonstrated, at very least, that STAT5 expression can directly activate the expression of milk protein genes, and particularly the WAP gene without PRL‐mediated signal transduction. Mol. Reprod. Dev. 54:121–125, 1999. © 1999 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Artificial Gene Fusion</subject><subject>Biological and medical sciences</subject><subject>Breast - metabolism</subject><subject>Caseins - genetics</subject><subject>Caseins - metabolism</subject><subject>DNA-Binding Proteins - pharmacology</subject><subject>DNA-Binding Proteins - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression - drug effects</subject><subject>Gene Expression - physiology</subject><subject>genes</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred ICR</subject><subject>Mice, Transgenic</subject><subject>milk proteins</subject><subject>Milk Proteins - genetics</subject><subject>Milk Proteins - metabolism</subject><subject>Models, Genetic</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>ovine STAT5</subject><subject>prolactin</subject><subject>Prolactin - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>signal transducer for activating transcription</subject><subject>signal transduction</subject><subject>Signal Transduction - physiology</subject><subject>STAT5 Transcription Factor</subject><subject>Trans-Activators - pharmacology</subject><subject>Trans-Activators - physiology</subject><subject>transgenic animals</subject><subject>transgenic mice</subject><subject>WAP</subject><subject>Whey Proteins</subject><subject>β-casein</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd9u0zAUxiMEYmPwCpALhLaLlOM_aewyIVXtGJUGRWsmEDdHjuN0ZmlS4lRrH4G3xlmqDQkkJEux4-_8zufzBcEpgQEBoG-PF7PJ7ISAFBFNZHxMpJQETmI-oqeEktFoPJtGny6n7D0bwGAyf0ej9FFweF_wuNtziHhMvx0Ez5z7AQBSCngaHPiLhPh1GPw6264b45ytq7AuwpUtb8J1U7fGVuHSVMaF1q8q32iTh7ltjG7LXZjtQrOt_X29ceEiHadxeGvb63rTdsWl0q2topXJrWp9mbPLSpVh26jKeVDb9fL4u7NnWO3bavM8eFKo0pkX--9RcPXhLJ18jC7m57PJ-CLSMREsEkoIafiQiEIySKQq4iHNizxjKs8yZagmlEmqEi65IQllRaaBDzPgOQwznrOj4E3P9U5_boxrcWWdNmWpKuOfgwkAAZEwL0x7oW5q5xpT4LqxK9XskAB2ESF2EWE3cewmjn1EGHOk6CNC9BFhFxEyBJzM_e_UY1_u-28yP6I_oH0mXvB6L1BOq7LwY9LWPeik4DGIB3u3tjS7v7z9x9o_nN2dPTbqsda1ZnuPVc0NDhOWxPj18zmKL4Kk0-klfvf6V72-UDWqZeOdXi0oEAZUxlRwwn4DNzPTaA</recordid><startdate>199910</startdate><enddate>199910</enddate><creator>Inuzuka, H</creator><creator>Yamanouchi, K</creator><creator>Tachi, C</creator><creator>Tojo, H</creator><general>John Wiley & Sons, Inc</general><general>Wiley-Liss</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199910</creationdate><title>Expression of milk protein genes is induced directly by exogenous STAT5 without prolactin-mediated signal transduction in transgenic mice</title><author>Inuzuka, H ; Yamanouchi, K ; Tachi, C ; Tojo, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5183-8a889e4618f93079af562dfdb3adbbae2c12392a7494e1723fbc046b04d06b4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Artificial Gene Fusion</topic><topic>Biological and medical sciences</topic><topic>Breast - metabolism</topic><topic>Caseins - genetics</topic><topic>Caseins - metabolism</topic><topic>DNA-Binding Proteins - pharmacology</topic><topic>DNA-Binding Proteins - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression - drug effects</topic><topic>Gene Expression - physiology</topic><topic>genes</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred ICR</topic><topic>Mice, Transgenic</topic><topic>milk proteins</topic><topic>Milk Proteins - genetics</topic><topic>Milk Proteins - metabolism</topic><topic>Models, Genetic</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>ovine STAT5</topic><topic>prolactin</topic><topic>Prolactin - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>signal transducer for activating transcription</topic><topic>signal transduction</topic><topic>Signal Transduction - physiology</topic><topic>STAT5 Transcription Factor</topic><topic>Trans-Activators - pharmacology</topic><topic>Trans-Activators - physiology</topic><topic>transgenic animals</topic><topic>transgenic mice</topic><topic>WAP</topic><topic>Whey Proteins</topic><topic>β-casein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Inuzuka, H</creatorcontrib><creatorcontrib>Yamanouchi, K</creatorcontrib><creatorcontrib>Tachi, C</creatorcontrib><creatorcontrib>Tojo, H</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inuzuka, H</au><au>Yamanouchi, K</au><au>Tachi, C</au><au>Tojo, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of milk protein genes is induced directly by exogenous STAT5 without prolactin-mediated signal transduction in transgenic mice</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol. Reprod. Dev</addtitle><date>1999-10</date><risdate>1999</risdate><volume>54</volume><issue>2</issue><spage>121</spage><epage>125</epage><pages>121-125</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><coden>MREDEE</coden><abstract>STAT5 is a member of the family of STAT (signal transducer for activating transcription), which was isolated from nuclear extracts of the lactating gland of sheep. It was reported that, in vitro, STAT5 could be activated directly by prolactin (PRL) without PRL‐mediated signal transduction. The present study was conducted to investigate above the possibility in vivo, using the transgenic mice overexpressing ovine STAT5 (oSTAT5) under the control of MMTV promoter. Three transgenic mouse lines that expressed the exogenously introduced oSTAT5 in their mammary glands were established. The expression levels of exogenous oSTAT5 were higher than those of endogenous STAT in the mammary glands of all of the three transgenic lines. Although the expression levels of endogenous milk protein genes, WAP, and β‐casein genes, were not correlated with oSTAT5 expression, the expression levels of WAP and β‐casein were induced by exogenous oSTAT5 in the transgenic mice. The present study demonstrated, at very least, that STAT5 expression can directly activate the expression of milk protein genes, and particularly the WAP gene without PRL‐mediated signal transduction. Mol. Reprod. Dev. 54:121–125, 1999. © 1999 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>10471471</pmid><doi>10.1002/(SICI)1098-2795(199910)54:2<121::AID-MRD3>3.0.CO;2-T</doi><tpages>5</tpages></addata></record> |
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| ispartof | Molecular reproduction and development, 1999-10, Vol.54 (2), p.121-125 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Artificial Gene Fusion Biological and medical sciences Breast - metabolism Caseins - genetics Caseins - metabolism DNA-Binding Proteins - pharmacology DNA-Binding Proteins - physiology Fundamental and applied biological sciences. Psychology Gene expression Gene Expression - drug effects Gene Expression - physiology genes Mice Mice, Inbred C57BL Mice, Inbred ICR Mice, Transgenic milk proteins Milk Proteins - genetics Milk Proteins - metabolism Models, Genetic Molecular and cellular biology Molecular genetics ovine STAT5 prolactin Prolactin - metabolism Reverse Transcriptase Polymerase Chain Reaction signal transducer for activating transcription signal transduction Signal Transduction - physiology STAT5 Transcription Factor Trans-Activators - pharmacology Trans-Activators - physiology transgenic animals transgenic mice WAP Whey Proteins β-casein |
| title | Expression of milk protein genes is induced directly by exogenous STAT5 without prolactin-mediated signal transduction in transgenic mice |
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