Overproduction of the Bradyrhizobium japonicum c-type cytochrome subunits of the cbb3 oxidase in Escherichia coli

We report on a system to improve expression of mature c-type cytochromes in Escherichia coli. It is based on the use of plasmid pEC86 that expresses the E. coli cytochrome c maturation genes ccmABCDEFGH constitutively, whereby the production of both endogenous and foreign c-type cytochromes was incr...

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Veröffentlicht in:	Biochemical and biophysical research communications 1998-10, Vol.251 (3), p.744-747
Hauptverfasser:	Arslan, E, Schulz, H, Zufferey, R, Künzler, P, Thöny-Meyer, L
Format:	Artikel
Sprache:	eng
Schlagworte:	Bradyrhizobium - enzymology Cytochrome c Group - biosynthesis Cytochrome c Group - genetics Electron Transport Complex IV - biosynthesis Electron Transport Complex IV - genetics Escherichia coli - genetics Membrane Proteins - biosynthesis Membrane Proteins - genetics Oxidoreductases - biosynthesis Oxidoreductases - genetics Plasmids Protein Processing, Post-Translational Recombinant Proteins - biosynthesis Solubility
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