Transcriptional profiling on all open reading frames of Saccharomyces cerevisiae
Open reading frames (6116) of the budding yeast Saccharomyces cerevisiae were PCR‐amplified from genomic DNA using 12,232 primers specific to the ends of the coding sequences; the success rate of amplification was 97%. PCR‐products were made accessible to hybridization by being arrayed at very high...
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| Veröffentlicht in: | Yeast (Chichester, England) England), 1998-09, Vol.14 (13), p.1209-1221 |
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| container_title | Yeast (Chichester, England) |
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| creator | Hauser, Nicole C. Vingron, Martin Scheideler, Marcel Krems, Bernhard Hellmuth, Klaus Entian, Karl‐Dieter Hoheisel, Jörg D. |
| description | Open reading frames (6116) of the budding yeast Saccharomyces cerevisiae were PCR‐amplified from genomic DNA using 12,232 primers specific to the ends of the coding sequences; the success rate of amplification was 97%. PCR‐products were made accessible to hybridization by being arrayed at very high density on solid support media using various robotic devices. Probes made from total RNA preparations were hybridized for the analysis of the transcriptional activity of yeast under various growth conditions and of different strains. Experimental factors that proved critical to the performance, such as different RNA isolation procedures and the assessment of hybridization results, for example, were investigated in detail. Various software tools were developed that permit convenient handling and sound analysis of the large data quantities obtained from transcriptional profiling studies. Comprehensive arrays are being distributed within the European Yeast Functional Analysis Network (EUROFAN) and beyond. © 1998 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/(SICI)1097-0061(19980930)14:13<1209::AID-YEA311>3.0.CO;2-N |
| format | Article |
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PCR‐products were made accessible to hybridization by being arrayed at very high density on solid support media using various robotic devices. Probes made from total RNA preparations were hybridized for the analysis of the transcriptional activity of yeast under various growth conditions and of different strains. Experimental factors that proved critical to the performance, such as different RNA isolation procedures and the assessment of hybridization results, for example, were investigated in detail. Various software tools were developed that permit convenient handling and sound analysis of the large data quantities obtained from transcriptional profiling studies. 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Comprehensive arrays are being distributed within the European Yeast Functional Analysis Network (EUROFAN) and beyond. © 1998 John Wiley & Sons, Ltd.</description><subject>Blotting, Western</subject><subject>DNA Primers - chemistry</subject><subject>DNA Probes - chemistry</subject><subject>Electrophoresis, Agar Gel</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enzymes - chemistry</subject><subject>expression profiling</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Image Processing, Computer-Assisted</subject><subject>microarrays</subject><subject>Nucleic Acid Hybridization</subject><subject>Open Reading Frames - genetics</subject><subject>Phenol - chemistry</subject><subject>Polymerase Chain Reaction</subject><subject>RNA, Fungal - chemistry</subject><subject>RNA, Fungal - isolation & purification</subject><subject>Robotics</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Sensitivity and Specificity</subject><subject>transcription</subject><subject>Transcription, Genetic - genetics</subject><subject>Transcription, Genetic - physiology</subject><issn>0749-503X</issn><issn>1097-0061</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9rE0EUxQdRalr9CMI-Sfuw8d6ZnexOFCFdaw2URmgFBeEymdzVkf3XmcaSb-8uSfvq04Vzzr1czk-Ic4QpAsh3pzfLcnmGYPIUYIanaEwBRsEZZnNUH1CCmc8Xy0_pj4uFQvyopjAtV-9lev1MTJ7WnosJ5JlJNajvL8VxjH8AELUsjsSRyQ0WRk7E19tg2-iC7-9919o66UNX-dq3v5KuTWxdJ13PbRLYbkatCrbhmHRVcmOd-21D1-zcIDgO_NdHb_mVeFHZOvLrwzwR3z5f3JZf0qvV5bJcXKW9khJTWWWOnTTKsimKtYIq07nOhn_VzHBmgAuUxazCYm3WXIHJHA5hyZoVON6oE_F2f3d4-G7L8Z4aHx3XtW2520bKAUBDrv4bxBz1DLUegm8Owe264Q31wTc27OjQ1eD_3PsPvubdk41AIzQamdFYPo3l0yMzwoxQ0ciMBmS0R0aKgMoVSbo-KOofIs6PIw</recordid><startdate>19980930</startdate><enddate>19980930</enddate><creator>Hauser, Nicole C.</creator><creator>Vingron, Martin</creator><creator>Scheideler, Marcel</creator><creator>Krems, Bernhard</creator><creator>Hellmuth, Klaus</creator><creator>Entian, Karl‐Dieter</creator><creator>Hoheisel, Jörg D.</creator><general>John Wiley & Sons, Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19980930</creationdate><title>Transcriptional profiling on all open reading frames of Saccharomyces cerevisiae</title><author>Hauser, Nicole C. ; Vingron, Martin ; Scheideler, Marcel ; Krems, Bernhard ; Hellmuth, Klaus ; Entian, Karl‐Dieter ; Hoheisel, Jörg D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p3221-2f4cec293ae988b30f45754749369e490e81286f18b9bef094c193a2e5e30ced3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Blotting, Western</topic><topic>DNA Primers - chemistry</topic><topic>DNA Probes - chemistry</topic><topic>Electrophoresis, Agar Gel</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Enzymes - chemistry</topic><topic>expression profiling</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Image Processing, Computer-Assisted</topic><topic>microarrays</topic><topic>Nucleic Acid Hybridization</topic><topic>Open Reading Frames - genetics</topic><topic>Phenol - chemistry</topic><topic>Polymerase Chain Reaction</topic><topic>RNA, Fungal - chemistry</topic><topic>RNA, Fungal - isolation & purification</topic><topic>Robotics</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Sensitivity and Specificity</topic><topic>transcription</topic><topic>Transcription, Genetic - genetics</topic><topic>Transcription, Genetic - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hauser, Nicole C.</creatorcontrib><creatorcontrib>Vingron, Martin</creatorcontrib><creatorcontrib>Scheideler, Marcel</creatorcontrib><creatorcontrib>Krems, Bernhard</creatorcontrib><creatorcontrib>Hellmuth, Klaus</creatorcontrib><creatorcontrib>Entian, Karl‐Dieter</creatorcontrib><creatorcontrib>Hoheisel, Jörg D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Yeast (Chichester, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hauser, Nicole C.</au><au>Vingron, Martin</au><au>Scheideler, Marcel</au><au>Krems, Bernhard</au><au>Hellmuth, Klaus</au><au>Entian, Karl‐Dieter</au><au>Hoheisel, Jörg D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptional profiling on all open reading frames of Saccharomyces cerevisiae</atitle><jtitle>Yeast (Chichester, England)</jtitle><addtitle>Yeast</addtitle><date>1998-09-30</date><risdate>1998</risdate><volume>14</volume><issue>13</issue><spage>1209</spage><epage>1221</epage><pages>1209-1221</pages><issn>0749-503X</issn><eissn>1097-0061</eissn><abstract>Open reading frames (6116) of the budding yeast Saccharomyces cerevisiae were PCR‐amplified from genomic DNA using 12,232 primers specific to the ends of the coding sequences; the success rate of amplification was 97%. PCR‐products were made accessible to hybridization by being arrayed at very high density on solid support media using various robotic devices. Probes made from total RNA preparations were hybridized for the analysis of the transcriptional activity of yeast under various growth conditions and of different strains. Experimental factors that proved critical to the performance, such as different RNA isolation procedures and the assessment of hybridization results, for example, were investigated in detail. Various software tools were developed that permit convenient handling and sound analysis of the large data quantities obtained from transcriptional profiling studies. Comprehensive arrays are being distributed within the European Yeast Functional Analysis Network (EUROFAN) and beyond. © 1998 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>9791892</pmid><doi>10.1002/(SICI)1097-0061(19980930)14:13<1209::AID-YEA311>3.0.CO;2-N</doi><tpages>13</tpages></addata></record> |
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| subjects | Blotting, Western DNA Primers - chemistry DNA Probes - chemistry Electrophoresis, Agar Gel Electrophoresis, Polyacrylamide Gel Enzymes - chemistry expression profiling Gene Expression Regulation, Fungal Image Processing, Computer-Assisted microarrays Nucleic Acid Hybridization Open Reading Frames - genetics Phenol - chemistry Polymerase Chain Reaction RNA, Fungal - chemistry RNA, Fungal - isolation & purification Robotics Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Sensitivity and Specificity transcription Transcription, Genetic - genetics Transcription, Genetic - physiology |
| title | Transcriptional profiling on all open reading frames of Saccharomyces cerevisiae |
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