Detection by HPLC of a trypanothione synthetase activity in vitro from Entamoeba histolytica

We have previously demonstrated the presence of glutathione‐spermidine (Gsp) and trypanothione [T(SH)2] from Entamoeba histolytica trophozoites, on the basis of results obtained with acid extracts purified by Florisil and DEAE‐cellulose, derivatized with the fluorescent reagent monobromobimane and s...

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Veröffentlicht in:	Biotechnology and applied biochemistry 1999-08, Vol.30 (1), p.41-45
Hauptverfasser:	Ondarza, Raul N., Hernandez, Eva, Iturbe, Angelica, Hurtado, Gerardo, Tamayo, Elsa Maria
Format:	Artikel
Sprache:	eng
Schlagworte:	Amide Synthases - metabolism Animals Biological and medical sciences Chromatography, High Pressure Liquid Chromatography, Ion Exchange Crithidia - enzymology Entamoeba histolytica Entamoeba histolytica - enzymology Fundamental and applied biological sciences. Psychology General aspects and techniques Protozoa Spectrometry, Fluorescence
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