Relationship between abnormal sperm chromatin packing and IVF results
This study was initiated to determine the relationship between the fertilizing potential of spermatozoa and abnormalities in the compact packing of their chromatin which occurs in the final stage of male germ cell differentiation. Chromatin packing involves disulphide bridge covalent cross-linking....
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| Veröffentlicht in: | Molecular human reproduction 1999-09, Vol.5 (9), p.825-830 |
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| container_title | Molecular human reproduction |
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| creator | Filatov, M V Semenova, E V Vorob'eva, O A Leont'eva, O A Drobchenko, E A |
| description | This study was initiated to determine the relationship between the fertilizing potential of spermatozoa and abnormalities in the compact packing of their chromatin which occurs in the final stage of male germ cell differentiation. Chromatin packing involves disulphide bridge covalent cross-linking. The degree of packing was determined from the accessibility of DNA to a fluorescent dye, ethidium bromide, following detergent treatment of the spermatozoa. The amount of dye bound was determined by flow cytometry in the presence or absence of heparin, a polyanion which removes only non-disulphide bridge-linked proteins. The results of a number of different sperm samples were compared with their results following in-vitro fertilization, and a relationship between disordered sperm chromatin packing and rates of embryo cleavage was observed. This study suggests that abnormal chromatin packing in spermatozoa may contribute to male fertility. |
| doi_str_mv | 10.1093/molehr/5.9.825 |
| format | Article |
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This study suggests that abnormal chromatin packing in spermatozoa may contribute to male fertility.</description><identifier>ISSN: 1360-9947</identifier><identifier>EISSN: 1460-2407</identifier><identifier>DOI: 10.1093/molehr/5.9.825</identifier><identifier>PMID: 10460220</identifier><language>eng</language><publisher>England</publisher><subject>Cell Nucleus - genetics ; Cell Nucleus - ultrastructure ; Chromatin - metabolism ; Chromatin - ultrastructure ; DNA - metabolism ; Embryo Transfer ; Embryo, Mammalian - physiology ; Ethidium - analysis ; Ethidium - metabolism ; Female ; Fertilization in Vitro - methods ; Flow Cytometry ; Fluorescent Dyes - analysis ; Fluorescent Dyes - metabolism ; Humans ; Infertility, Male - genetics ; Male ; Molecular Biology - methods ; Nuclear Proteins - metabolism ; Oligospermia ; Pregnancy ; Pregnancy Rate ; Reference Values ; Spermatozoa - cytology ; Spermatozoa - physiology</subject><ispartof>Molecular human reproduction, 1999-09, Vol.5 (9), p.825-830</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c331t-aebc8229e6d0ca055228af95cf8a0b02126070a17063e51885bdad6123149fae3</citedby><cites>FETCH-LOGICAL-c331t-aebc8229e6d0ca055228af95cf8a0b02126070a17063e51885bdad6123149fae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10460220$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Filatov, M V</creatorcontrib><creatorcontrib>Semenova, E V</creatorcontrib><creatorcontrib>Vorob'eva, O A</creatorcontrib><creatorcontrib>Leont'eva, O A</creatorcontrib><creatorcontrib>Drobchenko, E A</creatorcontrib><title>Relationship between abnormal sperm chromatin packing and IVF results</title><title>Molecular human reproduction</title><addtitle>Mol Hum Reprod</addtitle><description>This study was initiated to determine the relationship between the fertilizing potential of spermatozoa and abnormalities in the compact packing of their chromatin which occurs in the final stage of male germ cell differentiation. 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This study suggests that abnormal chromatin packing in spermatozoa may contribute to male fertility.</description><subject>Cell Nucleus - genetics</subject><subject>Cell Nucleus - ultrastructure</subject><subject>Chromatin - metabolism</subject><subject>Chromatin - ultrastructure</subject><subject>DNA - metabolism</subject><subject>Embryo Transfer</subject><subject>Embryo, Mammalian - physiology</subject><subject>Ethidium - analysis</subject><subject>Ethidium - metabolism</subject><subject>Female</subject><subject>Fertilization in Vitro - methods</subject><subject>Flow Cytometry</subject><subject>Fluorescent Dyes - analysis</subject><subject>Fluorescent Dyes - metabolism</subject><subject>Humans</subject><subject>Infertility, Male - genetics</subject><subject>Male</subject><subject>Molecular Biology - methods</subject><subject>Nuclear Proteins - metabolism</subject><subject>Oligospermia</subject><subject>Pregnancy</subject><subject>Pregnancy Rate</subject><subject>Reference Values</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - physiology</subject><issn>1360-9947</issn><issn>1460-2407</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkDFPwzAUhC0EolBYGZEntqTPdpzEI6paqFQJCQGr5TgvNBAnwU6E-PcEpQPT3fDdDR8hNwxiBkqsXNfgwa9krOKcyxNywZIUIp5Adjp1MXWlkmxBLkP4AGAZl_k5WTCYKM7hgmyesTFD3bXhUPe0wOEbsaWmaDvvTENDj95Re_Cdm6iW9sZ-1u07NW1Jd29b6jGMzRCuyFllmoDXx1yS1-3mZf0Y7Z8eduv7fWSFYENksLA55wrTEqwBKTnPTaWkrXIDBXDGU8jAsAxSgZLluSxKU6aMC5aoyqBYkrv5t_fd14hh0K4OFpvGtNiNQadKKSFEOoHxDFrfheCx0r2vnfE_moH-E6dncVpqpSdx0-D2-DwWDst_-GxK_AKs02qJ</recordid><startdate>19990901</startdate><enddate>19990901</enddate><creator>Filatov, M V</creator><creator>Semenova, E V</creator><creator>Vorob'eva, O A</creator><creator>Leont'eva, O A</creator><creator>Drobchenko, E A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990901</creationdate><title>Relationship between abnormal sperm chromatin packing and IVF results</title><author>Filatov, M V ; Semenova, E V ; Vorob'eva, O A ; Leont'eva, O A ; Drobchenko, E A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c331t-aebc8229e6d0ca055228af95cf8a0b02126070a17063e51885bdad6123149fae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Cell Nucleus - genetics</topic><topic>Cell Nucleus - ultrastructure</topic><topic>Chromatin - metabolism</topic><topic>Chromatin - ultrastructure</topic><topic>DNA - metabolism</topic><topic>Embryo Transfer</topic><topic>Embryo, Mammalian - physiology</topic><topic>Ethidium - analysis</topic><topic>Ethidium - metabolism</topic><topic>Female</topic><topic>Fertilization in Vitro - methods</topic><topic>Flow Cytometry</topic><topic>Fluorescent Dyes - analysis</topic><topic>Fluorescent Dyes - metabolism</topic><topic>Humans</topic><topic>Infertility, Male - genetics</topic><topic>Male</topic><topic>Molecular Biology - methods</topic><topic>Nuclear Proteins - metabolism</topic><topic>Oligospermia</topic><topic>Pregnancy</topic><topic>Pregnancy Rate</topic><topic>Reference Values</topic><topic>Spermatozoa - cytology</topic><topic>Spermatozoa - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Filatov, M V</creatorcontrib><creatorcontrib>Semenova, E V</creatorcontrib><creatorcontrib>Vorob'eva, O A</creatorcontrib><creatorcontrib>Leont'eva, O A</creatorcontrib><creatorcontrib>Drobchenko, E A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular human reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Filatov, M V</au><au>Semenova, E V</au><au>Vorob'eva, O A</au><au>Leont'eva, O A</au><au>Drobchenko, E A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relationship between abnormal sperm chromatin packing and IVF results</atitle><jtitle>Molecular human reproduction</jtitle><addtitle>Mol Hum Reprod</addtitle><date>1999-09-01</date><risdate>1999</risdate><volume>5</volume><issue>9</issue><spage>825</spage><epage>830</epage><pages>825-830</pages><issn>1360-9947</issn><eissn>1460-2407</eissn><abstract>This study was initiated to determine the relationship between the fertilizing potential of spermatozoa and abnormalities in the compact packing of their chromatin which occurs in the final stage of male germ cell differentiation. 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| ispartof | Molecular human reproduction, 1999-09, Vol.5 (9), p.825-830 |
| issn | 1360-9947 1460-2407 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; Oxford Journals |
| subjects | Cell Nucleus - genetics Cell Nucleus - ultrastructure Chromatin - metabolism Chromatin - ultrastructure DNA - metabolism Embryo Transfer Embryo, Mammalian - physiology Ethidium - analysis Ethidium - metabolism Female Fertilization in Vitro - methods Flow Cytometry Fluorescent Dyes - analysis Fluorescent Dyes - metabolism Humans Infertility, Male - genetics Male Molecular Biology - methods Nuclear Proteins - metabolism Oligospermia Pregnancy Pregnancy Rate Reference Values Spermatozoa - cytology Spermatozoa - physiology |
| title | Relationship between abnormal sperm chromatin packing and IVF results |
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