The Chick Embryo Chorioallantoic Membrane as a Model to Investigate the Angiogenic Properties of Human Endometrium
The chick embryo chorioallantoic membrane (CAM) is an established in vivo angiogenesis assay. The aim of our study was to assess the angiogenic properties of endometrium and to quantitate the vascular response in an accurate way. Samples of proliferative endometrium (n = 17) and control mouse skin t...
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| Veröffentlicht in: | Gynecologic and obstetric investigation 1999-01, Vol.48 (2), p.108-112 |
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| container_title | Gynecologic and obstetric investigation |
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| creator | Maas, Jacques W.M. Le Noble, Ferdinand A.C. Dunselman, Gerard A.J. de Goeij, Anton F.P.M. Struyker Boudier, Harry A.J. Evers, Johannes L.H. |
| description | The chick embryo chorioallantoic membrane (CAM) is an established in vivo angiogenesis assay. The aim of our study was to assess the angiogenic properties of endometrium and to quantitate the vascular response in an accurate way. Samples of proliferative endometrium (n = 17) and control mouse skin tissue (n = 8) were explanted onto the CAM at day 10 of incubation. Additional controls consisted of normal unmanipulated CAM (n = 12). Four days after grafting, photographs of the explant and the surrounding area were taken in ovo to measure the vascular density index (VDI). The VDI is a stereological estimate of vessel number and length, which was obtained by counting the intersections of vessels with a circular grid superimposed on a computerized image. Endometrium caused a significant increase in VDI as compared to both unmanipulated CAM (p < 0.001) and skin tissue as a control (p < 0.007). The intra-observer variability was 5.2%. This study demonstrates that the CAM assay is a suitable model to assess the angiogenic properties of endometrium. Furthermore, it allows detailed quantitation of the vascular response in an objective and reproducible way. Our findings suggest the CAM to be a promising model to study the role of angiogenesis in both normal human endometrium and diseases involving the endometrium. |
| doi_str_mv | 10.1159/000010150 |
| format | Article |
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The aim of our study was to assess the angiogenic properties of endometrium and to quantitate the vascular response in an accurate way. Samples of proliferative endometrium (n = 17) and control mouse skin tissue (n = 8) were explanted onto the CAM at day 10 of incubation. Additional controls consisted of normal unmanipulated CAM (n = 12). Four days after grafting, photographs of the explant and the surrounding area were taken in ovo to measure the vascular density index (VDI). The VDI is a stereological estimate of vessel number and length, which was obtained by counting the intersections of vessels with a circular grid superimposed on a computerized image. Endometrium caused a significant increase in VDI as compared to both unmanipulated CAM (p < 0.001) and skin tissue as a control (p < 0.007). The intra-observer variability was 5.2%. This study demonstrates that the CAM assay is a suitable model to assess the angiogenic properties of endometrium. Furthermore, it allows detailed quantitation of the vascular response in an objective and reproducible way. Our findings suggest the CAM to be a promising model to study the role of angiogenesis in both normal human endometrium and diseases involving the endometrium.</description><identifier>ISSN: 0378-7346</identifier><identifier>EISSN: 1423-002X</identifier><identifier>DOI: 10.1159/000010150</identifier><identifier>PMID: 10461001</identifier><identifier>CODEN: GOBIDS</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Allantois - blood supply ; Animals ; Biological and medical sciences ; Chick Embryo ; Chorion - blood supply ; Endometrium - blood supply ; Female ; Genital system. Mammary gland ; Humans ; Image Processing, Computer-Assisted ; In Vitro Techniques ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; Membranes ; Mice ; Models, Biological ; Neovascularization, Physiologic - physiology ; Observer Variation ; Original Paper ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Pregnancy ; Skin Transplantation ; Transplantation, Heterologous</subject><ispartof>Gynecologic and obstetric investigation, 1999-01, Vol.48 (2), p.108-112</ispartof><rights>1999 S. Karger AG, Basel</rights><rights>1999 INIST-CNRS</rights><rights>Copyright (c) 1999 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-7a6924a91a9f525cff8c488bb86b2668afda5df8bde161ac755c9cac731550d53</citedby><cites>FETCH-LOGICAL-c384t-7a6924a91a9f525cff8c488bb86b2668afda5df8bde161ac755c9cac731550d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,2423,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1905462$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10461001$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maas, Jacques W.M.</creatorcontrib><creatorcontrib>Le Noble, Ferdinand A.C.</creatorcontrib><creatorcontrib>Dunselman, Gerard A.J.</creatorcontrib><creatorcontrib>de Goeij, Anton F.P.M.</creatorcontrib><creatorcontrib>Struyker Boudier, Harry A.J.</creatorcontrib><creatorcontrib>Evers, Johannes L.H.</creatorcontrib><title>The Chick Embryo Chorioallantoic Membrane as a Model to Investigate the Angiogenic Properties of Human Endometrium</title><title>Gynecologic and obstetric investigation</title><addtitle>Gynecol Obstet Invest</addtitle><description>The chick embryo chorioallantoic membrane (CAM) is an established in vivo angiogenesis assay. The aim of our study was to assess the angiogenic properties of endometrium and to quantitate the vascular response in an accurate way. Samples of proliferative endometrium (n = 17) and control mouse skin tissue (n = 8) were explanted onto the CAM at day 10 of incubation. Additional controls consisted of normal unmanipulated CAM (n = 12). Four days after grafting, photographs of the explant and the surrounding area were taken in ovo to measure the vascular density index (VDI). The VDI is a stereological estimate of vessel number and length, which was obtained by counting the intersections of vessels with a circular grid superimposed on a computerized image. Endometrium caused a significant increase in VDI as compared to both unmanipulated CAM (p < 0.001) and skin tissue as a control (p < 0.007). The intra-observer variability was 5.2%. This study demonstrates that the CAM assay is a suitable model to assess the angiogenic properties of endometrium. Furthermore, it allows detailed quantitation of the vascular response in an objective and reproducible way. Our findings suggest the CAM to be a promising model to study the role of angiogenesis in both normal human endometrium and diseases involving the endometrium.</description><subject>Allantois - blood supply</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chick Embryo</subject><subject>Chorion - blood supply</subject><subject>Endometrium - blood supply</subject><subject>Female</subject><subject>Genital system. Mammary gland</subject><subject>Humans</subject><subject>Image Processing, Computer-Assisted</subject><subject>In Vitro Techniques</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Membranes</subject><subject>Mice</subject><subject>Models, Biological</subject><subject>Neovascularization, Physiologic - physiology</subject><subject>Observer Variation</subject><subject>Original Paper</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. 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Mammary gland</topic><topic>Humans</topic><topic>Image Processing, Computer-Assisted</topic><topic>In Vitro Techniques</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Membranes</topic><topic>Mice</topic><topic>Models, Biological</topic><topic>Neovascularization, Physiologic - physiology</topic><topic>Observer Variation</topic><topic>Original Paper</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Pregnancy</topic><topic>Skin Transplantation</topic><topic>Transplantation, Heterologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maas, Jacques W.M.</creatorcontrib><creatorcontrib>Le Noble, Ferdinand A.C.</creatorcontrib><creatorcontrib>Dunselman, Gerard A.J.</creatorcontrib><creatorcontrib>de Goeij, Anton F.P.M.</creatorcontrib><creatorcontrib>Struyker Boudier, Harry A.J.</creatorcontrib><creatorcontrib>Evers, Johannes L.H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Gynecologic and obstetric investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maas, Jacques W.M.</au><au>Le Noble, Ferdinand A.C.</au><au>Dunselman, Gerard A.J.</au><au>de Goeij, Anton F.P.M.</au><au>Struyker Boudier, Harry A.J.</au><au>Evers, Johannes L.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Chick Embryo Chorioallantoic Membrane as a Model to Investigate the Angiogenic Properties of Human Endometrium</atitle><jtitle>Gynecologic and obstetric investigation</jtitle><addtitle>Gynecol Obstet Invest</addtitle><date>1999-01-01</date><risdate>1999</risdate><volume>48</volume><issue>2</issue><spage>108</spage><epage>112</epage><pages>108-112</pages><issn>0378-7346</issn><eissn>1423-002X</eissn><coden>GOBIDS</coden><abstract>The chick embryo chorioallantoic membrane (CAM) is an established in vivo angiogenesis assay. The aim of our study was to assess the angiogenic properties of endometrium and to quantitate the vascular response in an accurate way. Samples of proliferative endometrium (n = 17) and control mouse skin tissue (n = 8) were explanted onto the CAM at day 10 of incubation. Additional controls consisted of normal unmanipulated CAM (n = 12). Four days after grafting, photographs of the explant and the surrounding area were taken in ovo to measure the vascular density index (VDI). The VDI is a stereological estimate of vessel number and length, which was obtained by counting the intersections of vessels with a circular grid superimposed on a computerized image. Endometrium caused a significant increase in VDI as compared to both unmanipulated CAM (p < 0.001) and skin tissue as a control (p < 0.007). The intra-observer variability was 5.2%. This study demonstrates that the CAM assay is a suitable model to assess the angiogenic properties of endometrium. Furthermore, it allows detailed quantitation of the vascular response in an objective and reproducible way. Our findings suggest the CAM to be a promising model to study the role of angiogenesis in both normal human endometrium and diseases involving the endometrium.</abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>10461001</pmid><doi>10.1159/000010150</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0378-7346 |
| ispartof | Gynecologic and obstetric investigation, 1999-01, Vol.48 (2), p.108-112 |
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| language | eng |
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| subjects | Allantois - blood supply Animals Biological and medical sciences Chick Embryo Chorion - blood supply Endometrium - blood supply Female Genital system. Mammary gland Humans Image Processing, Computer-Assisted In Vitro Techniques Investigative techniques, diagnostic techniques (general aspects) Medical sciences Membranes Mice Models, Biological Neovascularization, Physiologic - physiology Observer Variation Original Paper Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Pregnancy Skin Transplantation Transplantation, Heterologous |
| title | The Chick Embryo Chorioallantoic Membrane as a Model to Investigate the Angiogenic Properties of Human Endometrium |
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