Placental iodothyronine deiodinase III and II ratios, mRNA expression compared to enzyme activity
Iodothyronine deiodinases III and II (D3 and D2) specific enzyme activities in human placenta both decrease with gestational age. The relation of the enzyme activities with their respective mRNA expression was investigated by semi-quantitative RT-PCR on human placenta mRNA. To investigate if RT-PCR...
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| Veröffentlicht in: | Molecular and cellular endocrinology 1998-07, Vol.142 (1), p.67-73 |
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| creator | Stulp, Maykel R de Vijlder, Jan J.M Ris-Stalpers, Carrie |
| description | Iodothyronine deiodinases III and II (D3 and D2) specific enzyme activities in human placenta both decrease with gestational age. The relation of the enzyme activities with their respective mRNA expression was investigated by semi-quantitative RT-PCR on human placenta mRNA. To investigate if RT-PCR is a useful tool to detect iodothyronine deiodinase mRNA, several tissues were screened using this technique. In all tissues with iodothyronine deiodinase enzyme activity, the corresponding RT-PCR product is present. Similar to D3 specific enzyme activity, the amount of D3 mRNA in placenta declines with gestational age. The ratios of the D3/D2 enzyme activity and mRNA expression in placenta do not correlate. D3 enzyme activity shows an average 300-fold excess compared to D2 activity. However, semi-quantitative PCR analysis of D3 and D2 mRNA shows a D3/D2 ratio varying from 0.05 to 52. These results suggest that the placental D2 mRNA amplified is not translated into placental D2 enzyme activity. |
| doi_str_mv | 10.1016/S0303-7207(98)00118-X |
| format | Article |
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The relation of the enzyme activities with their respective mRNA expression was investigated by semi-quantitative RT-PCR on human placenta mRNA. To investigate if RT-PCR is a useful tool to detect iodothyronine deiodinase mRNA, several tissues were screened using this technique. In all tissues with iodothyronine deiodinase enzyme activity, the corresponding RT-PCR product is present. Similar to D3 specific enzyme activity, the amount of D3 mRNA in placenta declines with gestational age. The ratios of the D3/D2 enzyme activity and mRNA expression in placenta do not correlate. D3 enzyme activity shows an average 300-fold excess compared to D2 activity. However, semi-quantitative PCR analysis of D3 and D2 mRNA shows a D3/D2 ratio varying from 0.05 to 52. 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The relation of the enzyme activities with their respective mRNA expression was investigated by semi-quantitative RT-PCR on human placenta mRNA. To investigate if RT-PCR is a useful tool to detect iodothyronine deiodinase mRNA, several tissues were screened using this technique. In all tissues with iodothyronine deiodinase enzyme activity, the corresponding RT-PCR product is present. Similar to D3 specific enzyme activity, the amount of D3 mRNA in placenta declines with gestational age. The ratios of the D3/D2 enzyme activity and mRNA expression in placenta do not correlate. D3 enzyme activity shows an average 300-fold excess compared to D2 activity. However, semi-quantitative PCR analysis of D3 and D2 mRNA shows a D3/D2 ratio varying from 0.05 to 52. These results suggest that the placental D2 mRNA amplified is not translated into placental D2 enzyme activity.</description><subject>Female</subject><subject>Fetus - enzymology</subject><subject>Gene Expression</subject><subject>Gestational Age</subject><subject>Humans</subject><subject>Iodide Peroxidase - genetics</subject><subject>Iodide Peroxidase - metabolism</subject><subject>Iodothyronine Deiodinase Type II</subject><subject>Iodothyronine deiodinases</subject><subject>mRNA</subject><subject>Organ Specificity</subject><subject>Placenta (human)</subject><subject>Placenta - enzymology</subject><subject>Pregnancy</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>RT-PCR</subject><subject>Semi-quantitative RT-PCR</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9rGzEQxUVpSR23HyGgU2kg28ysvF7pVEzIH0NISppAbkKWxlRlV3Il2cT99NnEJtecHsO8mcf7MXaE8AMBp6e_QYCo2hra70oeAyDK6vEDG6Fs60pC035kozfLZ3aY818AaJtaHrAD1UqhYDJi5ldnLIViOu6ji-XPNsXgA3FHw-yDycTn8zk3wQ3Kkyk-5hPe393MOD2tEuXsY-A29iuTyPESOYX_2564scVvfNl-YZ-Wpsv0da9j9nBxfn92VV3fXs7PZteVFVMoFarlUKERDaC12JBFg5OFWExQCJwI6WChalColGhJSglWDfZaLKFRDrEVY_Zt93eV4r815aJ7ny11nQkU11lPlVKAsh6Mzc5oU8w50VKvku9N2moE_YJWv6LVL9y0kvoVrX4c7o72AetFT-7tas9y2P_c7WloufGUdLaegiXnE9miXfTvJDwDXmuHbQ</recordid><startdate>19980725</startdate><enddate>19980725</enddate><creator>Stulp, Maykel R</creator><creator>de Vijlder, Jan J.M</creator><creator>Ris-Stalpers, Carrie</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980725</creationdate><title>Placental iodothyronine deiodinase III and II ratios, mRNA expression compared to enzyme activity</title><author>Stulp, Maykel R ; de Vijlder, Jan J.M ; Ris-Stalpers, Carrie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-19f01153501cc15ec1a14b3b41331438d0b920919937e8880c911523f059d1173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Female</topic><topic>Fetus - enzymology</topic><topic>Gene Expression</topic><topic>Gestational Age</topic><topic>Humans</topic><topic>Iodide Peroxidase - genetics</topic><topic>Iodide Peroxidase - metabolism</topic><topic>Iodothyronine Deiodinase Type II</topic><topic>Iodothyronine deiodinases</topic><topic>mRNA</topic><topic>Organ Specificity</topic><topic>Placenta (human)</topic><topic>Placenta - enzymology</topic><topic>Pregnancy</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>RT-PCR</topic><topic>Semi-quantitative RT-PCR</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stulp, Maykel R</creatorcontrib><creatorcontrib>de Vijlder, Jan J.M</creatorcontrib><creatorcontrib>Ris-Stalpers, Carrie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stulp, Maykel R</au><au>de Vijlder, Jan J.M</au><au>Ris-Stalpers, Carrie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Placental iodothyronine deiodinase III and II ratios, mRNA expression compared to enzyme activity</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>1998-07-25</date><risdate>1998</risdate><volume>142</volume><issue>1</issue><spage>67</spage><epage>73</epage><pages>67-73</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><abstract>Iodothyronine deiodinases III and II (D3 and D2) specific enzyme activities in human placenta both decrease with gestational age. The relation of the enzyme activities with their respective mRNA expression was investigated by semi-quantitative RT-PCR on human placenta mRNA. To investigate if RT-PCR is a useful tool to detect iodothyronine deiodinase mRNA, several tissues were screened using this technique. In all tissues with iodothyronine deiodinase enzyme activity, the corresponding RT-PCR product is present. Similar to D3 specific enzyme activity, the amount of D3 mRNA in placenta declines with gestational age. The ratios of the D3/D2 enzyme activity and mRNA expression in placenta do not correlate. D3 enzyme activity shows an average 300-fold excess compared to D2 activity. However, semi-quantitative PCR analysis of D3 and D2 mRNA shows a D3/D2 ratio varying from 0.05 to 52. These results suggest that the placental D2 mRNA amplified is not translated into placental D2 enzyme activity.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>9783904</pmid><doi>10.1016/S0303-7207(98)00118-X</doi><tpages>7</tpages></addata></record> |
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| subjects | Female Fetus - enzymology Gene Expression Gestational Age Humans Iodide Peroxidase - genetics Iodide Peroxidase - metabolism Iodothyronine Deiodinase Type II Iodothyronine deiodinases mRNA Organ Specificity Placenta (human) Placenta - enzymology Pregnancy Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism RT-PCR Semi-quantitative RT-PCR |
| title | Placental iodothyronine deiodinase III and II ratios, mRNA expression compared to enzyme activity |
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