Immunity obtained by gene-gun inoculation of a rotavirus DNA vaccine to the abdominal epidermis or anorectal epithelium
We have previously shown that gene-gun delivery of murine rotavirus DNA vaccines to the epidermis induced protection against rotavirus challenge in mice. In this study, we used a rotavirus group antigen (VP6)-specific DNA vaccine to compare epidermal immunization with immunization to the anorectal e...
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| Veröffentlicht in: | Vaccine 1999-08, Vol.17 (23), p.3171-3176 |
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| creator | Chen, Shing C Fynan, Ellen F Greenberg, Harry B Herrmann, John E |
| description | We have previously shown that gene-gun delivery of murine rotavirus DNA vaccines to the epidermis induced protection against rotavirus challenge in mice. In this study, we used a rotavirus group antigen (VP6)-specific DNA vaccine to compare epidermal immunization with immunization to the anorectal epithelium for efficacy in inducing protective immunity. The vaccine was administered into cells of the abdominal epidermis or anorectal epithelium of adult BALB/c mice with an Accell gene-gun (PowderJect, Inc). Vaccines administered by either route elicited rotavirus-specific ELISA antibodies and analysis of the IgG subtypes indicated Th2-type responses were generated by both routes of administration, in contrast to Th1-type responses generated by live rotavirus. Protection against virus challenge was obtained in mice inoculated by either route, as shown by significant reduction of virus excreted in stools. The protection obtained by immunization of the anorectal epithelium was greater than that for epidermal immunization at the same vaccine dose. These results suggest that mucosal immunization of DNA vaccines may be an effective means to generate protective immunity against mucosal pathogens. |
| doi_str_mv | 10.1016/S0264-410X(99)00081-X |
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In this study, we used a rotavirus group antigen (VP6)-specific DNA vaccine to compare epidermal immunization with immunization to the anorectal epithelium for efficacy in inducing protective immunity. The vaccine was administered into cells of the abdominal epidermis or anorectal epithelium of adult BALB/c mice with an Accell gene-gun (PowderJect, Inc). Vaccines administered by either route elicited rotavirus-specific ELISA antibodies and analysis of the IgG subtypes indicated Th2-type responses were generated by both routes of administration, in contrast to Th1-type responses generated by live rotavirus. Protection against virus challenge was obtained in mice inoculated by either route, as shown by significant reduction of virus excreted in stools. The protection obtained by immunization of the anorectal epithelium was greater than that for epidermal immunization at the same vaccine dose. These results suggest that mucosal immunization of DNA vaccines may be an effective means to generate protective immunity against mucosal pathogens.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/S0264-410X(99)00081-X</identifier><identifier>PMID: 10462253</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Abdomen ; AIDS/HIV ; Animals ; Antibodies, Viral - biosynthesis ; Antibodies, Viral - blood ; Antibody Specificity ; Antigens, Viral - immunology ; Biolistics ; Biological and medical sciences ; Epidermis ; Epithelium ; Female ; Fundamental and applied biological sciences. Psychology ; Intestinal Mucosa - immunology ; Lymphocyte Activation - immunology ; Mice ; Mice, Inbred BALB C ; Microbiology ; Mucosal ; Plasmid DNA immunization ; Rectum ; Rotavirus ; Rotavirus - genetics ; Rotavirus - immunology ; Rotavirus Infections - prevention & control ; Th1 Cells - immunology ; Th2 Cells - immunology ; Vaccine ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vaccines, DNA - administration & dosage ; Vaccines, DNA - genetics ; Vaccines, DNA - immunology ; Viral Vaccines - administration & dosage ; Viral Vaccines - genetics ; Viral Vaccines - immunology ; Virology</subject><ispartof>Vaccine, 1999-08, Vol.17 (23), p.3171-3176</ispartof><rights>1999 Elsevier Science Ltd</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c516t-b128ba041b7207f6fc748c6a76e8eec3c59614ddab5c28726891042702d310193</citedby><cites>FETCH-LOGICAL-c516t-b128ba041b7207f6fc748c6a76e8eec3c59614ddab5c28726891042702d310193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0264-410X(99)00081-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1903930$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10462253$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Shing C</creatorcontrib><creatorcontrib>Fynan, Ellen F</creatorcontrib><creatorcontrib>Greenberg, Harry B</creatorcontrib><creatorcontrib>Herrmann, John E</creatorcontrib><title>Immunity obtained by gene-gun inoculation of a rotavirus DNA vaccine to the abdominal epidermis or anorectal epithelium</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>We have previously shown that gene-gun delivery of murine rotavirus DNA vaccines to the epidermis induced protection against rotavirus challenge in mice. In this study, we used a rotavirus group antigen (VP6)-specific DNA vaccine to compare epidermal immunization with immunization to the anorectal epithelium for efficacy in inducing protective immunity. The vaccine was administered into cells of the abdominal epidermis or anorectal epithelium of adult BALB/c mice with an Accell gene-gun (PowderJect, Inc). Vaccines administered by either route elicited rotavirus-specific ELISA antibodies and analysis of the IgG subtypes indicated Th2-type responses were generated by both routes of administration, in contrast to Th1-type responses generated by live rotavirus. Protection against virus challenge was obtained in mice inoculated by either route, as shown by significant reduction of virus excreted in stools. The protection obtained by immunization of the anorectal epithelium was greater than that for epidermal immunization at the same vaccine dose. These results suggest that mucosal immunization of DNA vaccines may be an effective means to generate protective immunity against mucosal pathogens.</description><subject>Abdomen</subject><subject>AIDS/HIV</subject><subject>Animals</subject><subject>Antibodies, Viral - biosynthesis</subject><subject>Antibodies, Viral - blood</subject><subject>Antibody Specificity</subject><subject>Antigens, Viral - immunology</subject><subject>Biolistics</subject><subject>Biological and medical sciences</subject><subject>Epidermis</subject><subject>Epithelium</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Intestinal Mucosa - immunology</subject><subject>Lymphocyte Activation - immunology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Mucosal</subject><subject>Plasmid DNA immunization</subject><subject>Rectum</subject><subject>Rotavirus</subject><subject>Rotavirus - genetics</subject><subject>Rotavirus - immunology</subject><subject>Rotavirus Infections - prevention & control</subject><subject>Th1 Cells - immunology</subject><subject>Th2 Cells - immunology</subject><subject>Vaccine</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vaccines, DNA - administration & dosage</subject><subject>Vaccines, DNA - genetics</subject><subject>Vaccines, DNA - immunology</subject><subject>Viral Vaccines - administration & dosage</subject><subject>Viral Vaccines - genetics</subject><subject>Viral Vaccines - immunology</subject><subject>Virology</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0cuKFDEUBuAgitOOPoKShYguSnOpSiUrGcbbwKALFXoXUsmpMVKVtEmqpd_e9FSj7mYVCN_J5f8RekrJa0qoePOVMNE2LSXbl0q9IoRI2mzvoQ2VPW9YR-V9tPlLztCjnH9W1HGqHqIzSlrBWMc36PfVPC_BlwOOQzE-gMPDAd9AgOZmCdiHaJfJFB8DjiM2OMVi9j4tGb_7fIH3xto6g0vE5QdgM7g4-2AmDDvvIM0-45iwCTGBLet2dZNf5sfowWimDE9O6zn6_uH9t8tPzfWXj1eXF9eN7agozUCZHAxp6dAz0o9itH0rrTC9AAlgue2UoK1zZugskz0TUtW_sZ4wx2tMip-jF-u5uxR_LZCLro-yME0mQFyyFkpJKVpxJ6Q9py1VXYXdCm2KOScY9S752aSDpkQfq9G31ehj7lopfVuN3ta5Z6cLlmEG99_U2kUFz0_AZGumMZlgff7nFOGKk8rergxqbHsPSWfrIVhw_piydtHf8ZI_Hwur2w</recordid><startdate>19990806</startdate><enddate>19990806</enddate><creator>Chen, Shing C</creator><creator>Fynan, Ellen F</creator><creator>Greenberg, Harry B</creator><creator>Herrmann, John E</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19990806</creationdate><title>Immunity obtained by gene-gun inoculation of a rotavirus DNA vaccine to the abdominal epidermis or anorectal epithelium</title><author>Chen, Shing C ; Fynan, Ellen F ; Greenberg, Harry B ; Herrmann, John E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-b128ba041b7207f6fc748c6a76e8eec3c59614ddab5c28726891042702d310193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Abdomen</topic><topic>AIDS/HIV</topic><topic>Animals</topic><topic>Antibodies, Viral - biosynthesis</topic><topic>Antibodies, Viral - blood</topic><topic>Antibody Specificity</topic><topic>Antigens, Viral - immunology</topic><topic>Biolistics</topic><topic>Biological and medical sciences</topic><topic>Epidermis</topic><topic>Epithelium</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Intestinal Mucosa - immunology</topic><topic>Lymphocyte Activation - immunology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Mucosal</topic><topic>Plasmid DNA immunization</topic><topic>Rectum</topic><topic>Rotavirus</topic><topic>Rotavirus - genetics</topic><topic>Rotavirus - immunology</topic><topic>Rotavirus Infections - prevention & control</topic><topic>Th1 Cells - immunology</topic><topic>Th2 Cells - immunology</topic><topic>Vaccine</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vaccines, DNA - administration & dosage</topic><topic>Vaccines, DNA - genetics</topic><topic>Vaccines, DNA - immunology</topic><topic>Viral Vaccines - administration & dosage</topic><topic>Viral Vaccines - genetics</topic><topic>Viral Vaccines - immunology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Shing C</creatorcontrib><creatorcontrib>Fynan, Ellen F</creatorcontrib><creatorcontrib>Greenberg, Harry B</creatorcontrib><creatorcontrib>Herrmann, John E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Shing C</au><au>Fynan, Ellen F</au><au>Greenberg, Harry B</au><au>Herrmann, John E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunity obtained by gene-gun inoculation of a rotavirus DNA vaccine to the abdominal epidermis or anorectal epithelium</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>1999-08-06</date><risdate>1999</risdate><volume>17</volume><issue>23</issue><spage>3171</spage><epage>3176</epage><pages>3171-3176</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>We have previously shown that gene-gun delivery of murine rotavirus DNA vaccines to the epidermis induced protection against rotavirus challenge in mice. In this study, we used a rotavirus group antigen (VP6)-specific DNA vaccine to compare epidermal immunization with immunization to the anorectal epithelium for efficacy in inducing protective immunity. The vaccine was administered into cells of the abdominal epidermis or anorectal epithelium of adult BALB/c mice with an Accell gene-gun (PowderJect, Inc). Vaccines administered by either route elicited rotavirus-specific ELISA antibodies and analysis of the IgG subtypes indicated Th2-type responses were generated by both routes of administration, in contrast to Th1-type responses generated by live rotavirus. Protection against virus challenge was obtained in mice inoculated by either route, as shown by significant reduction of virus excreted in stools. The protection obtained by immunization of the anorectal epithelium was greater than that for epidermal immunization at the same vaccine dose. These results suggest that mucosal immunization of DNA vaccines may be an effective means to generate protective immunity against mucosal pathogens.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>10462253</pmid><doi>10.1016/S0264-410X(99)00081-X</doi><tpages>6</tpages></addata></record> |
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| subjects | Abdomen AIDS/HIV Animals Antibodies, Viral - biosynthesis Antibodies, Viral - blood Antibody Specificity Antigens, Viral - immunology Biolistics Biological and medical sciences Epidermis Epithelium Female Fundamental and applied biological sciences. Psychology Intestinal Mucosa - immunology Lymphocyte Activation - immunology Mice Mice, Inbred BALB C Microbiology Mucosal Plasmid DNA immunization Rectum Rotavirus Rotavirus - genetics Rotavirus - immunology Rotavirus Infections - prevention & control Th1 Cells - immunology Th2 Cells - immunology Vaccine Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, DNA - administration & dosage Vaccines, DNA - genetics Vaccines, DNA - immunology Viral Vaccines - administration & dosage Viral Vaccines - genetics Viral Vaccines - immunology Virology |
| title | Immunity obtained by gene-gun inoculation of a rotavirus DNA vaccine to the abdominal epidermis or anorectal epithelium |
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