Nucleotide Sequence Variability in the Nontranscribed Spacer of the rRNA Locus in the Oyster Parasite Perkinsus marinus
We examined the sequence variability of the nontranscribed spacer (NTS) and internal-transcribed spacer (ITS1 and ITS2) domains of the rRNA locus of Perkinsus marinus from Maryland, Florida, and Louisiana. The sequence of P. marinus DNA including the 5S rRNA, NTS, small subunit (SSU) rRNA, ITS1, and...
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Veröffentlicht in: | The Journal of parasitology 1999-08, Vol.85 (4), p.650-656 |
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description | We examined the sequence variability of the nontranscribed spacer (NTS) and internal-transcribed spacer (ITS1 and ITS2) domains of the rRNA locus of Perkinsus marinus from Maryland, Florida, and Louisiana. The sequence of P. marinus DNA including the 5S rRNA, NTS, small subunit (SSU) rRNA, ITS1, and ITS2 regions confirmed their contiguity in the rRNA locus and revealed differences at 28 positions with the SSU rRNA sequences published earlier. The 307-bp polymerase chain reaction (PCR)-amplified fragments from the NTS domain of the various P. marinus isolates revealed the presence of 2 distinct sequences, designated as types I and II, that differed at 6 defined nucleotide positions. Based on these differences, nested PCR and restriction enzyme digests were used to distinguish between the 2 types. Sequences of the ITS1 and ITS2 domains of samples from either NTS type I (n = 3) or type II (n = 3) showed no variation and were identical to published sequences. Frequencies of the P. marinus NTS sequence types I and II in infected oysters varied with the geographic origin of the samples. All Maryland samples examined (n = 19) corresponded to the NTS type I sequence, the type II was the most frequent in the Florida samples (n = 17), and both types were about equally represented in the Louisiana samples (n = 19), with both sequence types found in individual oyster specimens. Although it has been suggested that P. marinus is diploid, it remains to be determined if both NTS sequence types can be present in a single P. marinus trophozoite. |
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F. Robledo ; Wright, Anita C. ; Marsh, Adam G. ; Vasta, Gerardo R.</creator><creatorcontrib>José A. F. Robledo ; Wright, Anita C. ; Marsh, Adam G. ; Vasta, Gerardo R.</creatorcontrib><description>We examined the sequence variability of the nontranscribed spacer (NTS) and internal-transcribed spacer (ITS1 and ITS2) domains of the rRNA locus of Perkinsus marinus from Maryland, Florida, and Louisiana. The sequence of P. marinus DNA including the 5S rRNA, NTS, small subunit (SSU) rRNA, ITS1, and ITS2 regions confirmed their contiguity in the rRNA locus and revealed differences at 28 positions with the SSU rRNA sequences published earlier. The 307-bp polymerase chain reaction (PCR)-amplified fragments from the NTS domain of the various P. marinus isolates revealed the presence of 2 distinct sequences, designated as types I and II, that differed at 6 defined nucleotide positions. Based on these differences, nested PCR and restriction enzyme digests were used to distinguish between the 2 types. Sequences of the ITS1 and ITS2 domains of samples from either NTS type I (n = 3) or type II (n = 3) showed no variation and were identical to published sequences. Frequencies of the P. marinus NTS sequence types I and II in infected oysters varied with the geographic origin of the samples. All Maryland samples examined (n = 19) corresponded to the NTS type I sequence, the type II was the most frequent in the Florida samples (n = 17), and both types were about equally represented in the Louisiana samples (n = 19), with both sequence types found in individual oyster specimens. Although it has been suggested that P. marinus is diploid, it remains to be determined if both NTS sequence types can be present in a single P. marinus trophozoite.</description><identifier>ISSN: 0022-3395</identifier><identifier>EISSN: 1937-2345</identifier><identifier>DOI: 10.2307/3285738</identifier><identifier>PMID: 10461944</identifier><identifier>CODEN: JOPAA2</identifier><language>eng</language><publisher>Lawrence, KS: American Society of Parasitologists</publisher><subject>Animals ; Apicomplexa - genetics ; Base Sequence ; Biological and medical sciences ; Dinoflagellida - genetics ; DNA ; DNA, Ribosomal - genetics ; Florida ; Fundamental and applied biological sciences. Psychology ; Genetic Variation ; Genetics of eukaryotes. Biological and molecular evolution ; Genetics-Evolution ; Geographic regions ; Gulfs ; Internal transcribed spacers ; Louisiana ; Marine ; Maryland ; Molecular Sequence Data ; Mollusca ; Ostreidae - parasitology ; Oysters ; Parasites ; Parasitology ; Perkinsus marinus ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Population genetics, reproduction patterns ; Protozoa ; rRNA genes ; Sequence Homology, Nucleic Acid ; Shellfish ; USA</subject><ispartof>The Journal of parasitology, 1999-08, Vol.85 (4), p.650-656</ispartof><rights>Copyright 1999 American Society of Parasitologists</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-19fd9816fec34061cbd806be9e1951779dda8c2148743b3fa3e5a159f8eb21463</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3285738$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3285738$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1927251$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10461944$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>José A. F. Robledo</creatorcontrib><creatorcontrib>Wright, Anita C.</creatorcontrib><creatorcontrib>Marsh, Adam G.</creatorcontrib><creatorcontrib>Vasta, Gerardo R.</creatorcontrib><title>Nucleotide Sequence Variability in the Nontranscribed Spacer of the rRNA Locus in the Oyster Parasite Perkinsus marinus</title><title>The Journal of parasitology</title><addtitle>J Parasitol</addtitle><description>We examined the sequence variability of the nontranscribed spacer (NTS) and internal-transcribed spacer (ITS1 and ITS2) domains of the rRNA locus of Perkinsus marinus from Maryland, Florida, and Louisiana. The sequence of P. marinus DNA including the 5S rRNA, NTS, small subunit (SSU) rRNA, ITS1, and ITS2 regions confirmed their contiguity in the rRNA locus and revealed differences at 28 positions with the SSU rRNA sequences published earlier. The 307-bp polymerase chain reaction (PCR)-amplified fragments from the NTS domain of the various P. marinus isolates revealed the presence of 2 distinct sequences, designated as types I and II, that differed at 6 defined nucleotide positions. Based on these differences, nested PCR and restriction enzyme digests were used to distinguish between the 2 types. Sequences of the ITS1 and ITS2 domains of samples from either NTS type I (n = 3) or type II (n = 3) showed no variation and were identical to published sequences. Frequencies of the P. marinus NTS sequence types I and II in infected oysters varied with the geographic origin of the samples. All Maryland samples examined (n = 19) corresponded to the NTS type I sequence, the type II was the most frequent in the Florida samples (n = 17), and both types were about equally represented in the Louisiana samples (n = 19), with both sequence types found in individual oyster specimens. Although it has been suggested that P. marinus is diploid, it remains to be determined if both NTS sequence types can be present in a single P. marinus trophozoite.</description><subject>Animals</subject><subject>Apicomplexa - genetics</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Dinoflagellida - genetics</subject><subject>DNA</subject><subject>DNA, Ribosomal - genetics</subject><subject>Florida</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Variation</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Genetics-Evolution</subject><subject>Geographic regions</subject><subject>Gulfs</subject><subject>Internal transcribed spacers</subject><subject>Louisiana</subject><subject>Marine</subject><subject>Maryland</subject><subject>Molecular Sequence Data</subject><subject>Mollusca</subject><subject>Ostreidae - parasitology</subject><subject>Oysters</subject><subject>Parasites</subject><subject>Parasitology</subject><subject>Perkinsus marinus</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Population genetics, reproduction patterns</subject><subject>Protozoa</subject><subject>rRNA genes</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Shellfish</subject><subject>USA</subject><issn>0022-3395</issn><issn>1937-2345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0UtLAzEQB_AgitYqfgPJQfS0mse-cizFF5RWfF2XbHYWU7e7NZNF-u2NtqIX8RACmR__MDOEHHF2LiTLLqTIk0zmW2TAlcwiIeNkmwwYEyKSUiV7ZB9xzhhLwtkle5zFKVdxPCDv09400HlbAX2Atx5aA_RZO6tL21i_oral_gXotGu90y0aZ0uo6MNSG3C0q7-K7n46opPO9PjNZyv0oX6nnUbrgd6Be7UtBrAI2W2PB2Sn1g3C4eYekqery8fxTTSZXd-OR5PIxFL4iKu6UjlPazAyZik3ZZWztAQFXCU8y1RV6dwIHudZLEtZawmJ5omqcyjDayqH5HSdu3Rd6A59sbBooGl0C12PRapUnjIm_4U8k0pyJQI8W0PjOkQHdbF0NnS1KjgrPpdRbJYR5PEmsi8XUP1y6-kHcLIBGo1u6jBgY_HHKZGJhP-wOfrO_fndB-9nm_c</recordid><startdate>19990801</startdate><enddate>19990801</enddate><creator>José A. F. Robledo</creator><creator>Wright, Anita C.</creator><creator>Marsh, Adam G.</creator><creator>Vasta, Gerardo R.</creator><general>American Society of Parasitologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19990801</creationdate><title>Nucleotide Sequence Variability in the Nontranscribed Spacer of the rRNA Locus in the Oyster Parasite Perkinsus marinus</title><author>José A. F. Robledo ; Wright, Anita C. ; Marsh, Adam G. ; Vasta, Gerardo R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-19fd9816fec34061cbd806be9e1951779dda8c2148743b3fa3e5a159f8eb21463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Apicomplexa - genetics</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Dinoflagellida - genetics</topic><topic>DNA</topic><topic>DNA, Ribosomal - genetics</topic><topic>Florida</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Variation</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Genetics-Evolution</topic><topic>Geographic regions</topic><topic>Gulfs</topic><topic>Internal transcribed spacers</topic><topic>Louisiana</topic><topic>Marine</topic><topic>Maryland</topic><topic>Molecular Sequence Data</topic><topic>Mollusca</topic><topic>Ostreidae - parasitology</topic><topic>Oysters</topic><topic>Parasites</topic><topic>Parasitology</topic><topic>Perkinsus marinus</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Population genetics, reproduction patterns</topic><topic>Protozoa</topic><topic>rRNA genes</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Shellfish</topic><topic>USA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>José A. F. Robledo</creatorcontrib><creatorcontrib>Wright, Anita C.</creatorcontrib><creatorcontrib>Marsh, Adam G.</creatorcontrib><creatorcontrib>Vasta, Gerardo R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>José A. F. Robledo</au><au>Wright, Anita C.</au><au>Marsh, Adam G.</au><au>Vasta, Gerardo R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nucleotide Sequence Variability in the Nontranscribed Spacer of the rRNA Locus in the Oyster Parasite Perkinsus marinus</atitle><jtitle>The Journal of parasitology</jtitle><addtitle>J Parasitol</addtitle><date>1999-08-01</date><risdate>1999</risdate><volume>85</volume><issue>4</issue><spage>650</spage><epage>656</epage><pages>650-656</pages><issn>0022-3395</issn><eissn>1937-2345</eissn><coden>JOPAA2</coden><abstract>We examined the sequence variability of the nontranscribed spacer (NTS) and internal-transcribed spacer (ITS1 and ITS2) domains of the rRNA locus of Perkinsus marinus from Maryland, Florida, and Louisiana. The sequence of P. marinus DNA including the 5S rRNA, NTS, small subunit (SSU) rRNA, ITS1, and ITS2 regions confirmed their contiguity in the rRNA locus and revealed differences at 28 positions with the SSU rRNA sequences published earlier. The 307-bp polymerase chain reaction (PCR)-amplified fragments from the NTS domain of the various P. marinus isolates revealed the presence of 2 distinct sequences, designated as types I and II, that differed at 6 defined nucleotide positions. Based on these differences, nested PCR and restriction enzyme digests were used to distinguish between the 2 types. Sequences of the ITS1 and ITS2 domains of samples from either NTS type I (n = 3) or type II (n = 3) showed no variation and were identical to published sequences. Frequencies of the P. marinus NTS sequence types I and II in infected oysters varied with the geographic origin of the samples. All Maryland samples examined (n = 19) corresponded to the NTS type I sequence, the type II was the most frequent in the Florida samples (n = 17), and both types were about equally represented in the Louisiana samples (n = 19), with both sequence types found in individual oyster specimens. Although it has been suggested that P. marinus is diploid, it remains to be determined if both NTS sequence types can be present in a single P. marinus trophozoite.</abstract><cop>Lawrence, KS</cop><pub>American Society of Parasitologists</pub><pmid>10461944</pmid><doi>10.2307/3285738</doi><tpages>7</tpages></addata></record> |
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subjects | Animals Apicomplexa - genetics Base Sequence Biological and medical sciences Dinoflagellida - genetics DNA DNA, Ribosomal - genetics Florida Fundamental and applied biological sciences. Psychology Genetic Variation Genetics of eukaryotes. Biological and molecular evolution Genetics-Evolution Geographic regions Gulfs Internal transcribed spacers Louisiana Marine Maryland Molecular Sequence Data Mollusca Ostreidae - parasitology Oysters Parasites Parasitology Perkinsus marinus Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Population genetics, reproduction patterns Protozoa rRNA genes Sequence Homology, Nucleic Acid Shellfish USA |
title | Nucleotide Sequence Variability in the Nontranscribed Spacer of the rRNA Locus in the Oyster Parasite Perkinsus marinus |
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