Dose-dependent effects of IL-12 treatment to immune response induced after immunization with a recombinant respiratory syncytial virus (RSV) fusion protein fragment
The amino acid (aa) sequence 190–289 of the RSV fusion (F) glycoprotein expressed in insect cells (bF 190–289) has been shown to partially protect BALB/c mice and to prime for a Th2 cell response. We evaluated the effects of IL-12 treatment during antigen priming of bF 190–289 on immune response and...
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| Veröffentlicht in: | Vaccine 1999-08, Vol.17 (23), p.2983-2990 |
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| creator | Werle, Bettina Fromantin, Catherine Alexandre, Anne Kohli, Evelyne Pothier, Pierre |
| description | The amino acid (aa) sequence 190–289 of the RSV fusion (F) glycoprotein expressed in insect cells (bF
190–289) has been shown to partially protect BALB/c mice and to prime for a Th2 cell response. We evaluated the effects of IL-12 treatment during antigen priming of bF
190–289 on immune response and protective efficacy. Low doses of IL-12 (10 ng) reduced IL-4 and IL-5 secretion (but did not affect IL-10 production) and decreased inflammatory signs whereas high doses of IL-12 had no effects. In addition, IL-12 treatment did not improve resistance to RSV replication. These results suggest that IL-12 treatment attenuates Th2 response and Th2 associated pulmonary inflammatory response in a dose-dependent manner, without improving protective efficacy. |
| doi_str_mv | 10.1016/S0264-410X(99)00172-3 |
| format | Article |
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190–289) has been shown to partially protect BALB/c mice and to prime for a Th2 cell response. We evaluated the effects of IL-12 treatment during antigen priming of bF
190–289 on immune response and protective efficacy. Low doses of IL-12 (10 ng) reduced IL-4 and IL-5 secretion (but did not affect IL-10 production) and decreased inflammatory signs whereas high doses of IL-12 had no effects. In addition, IL-12 treatment did not improve resistance to RSV replication. These results suggest that IL-12 treatment attenuates Th2 response and Th2 associated pulmonary inflammatory response in a dose-dependent manner, without improving protective efficacy.</description><subject>AIDS/HIV</subject><subject>Animals</subject><subject>Antigens, Viral - immunology</subject><subject>Biological and medical sciences</subject><subject>Bronchoalveolar Lavage Fluid - cytology</subject><subject>Bronchoalveolar Lavage Fluid - immunology</subject><subject>CD4-CD8 Ratio</subject><subject>Dose-Response Relationship, Immunologic</subject><subject>Eosinophils - cytology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>HN Protein</topic><topic>IL-12</topic><topic>Immune response</topic><topic>Interleukin-10 - biosynthesis</topic><topic>Interleukin-12 - pharmacology</topic><topic>Interleukin-4 - biosynthesis</topic><topic>Interleukin-5 - biosynthesis</topic><topic>Leukocyte Count</topic><topic>Lymphocyte Activation - immunology</topic><topic>Macrophages - cytology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Neutralization Tests</topic><topic>Peptide Fragments - immunology</topic><topic>Respiratory syncytial virus</topic><topic>Respiratory Syncytial Viruses - immunology</topic><topic>Th2 Cells - immunology</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Viral Envelope Proteins</topic><topic>Viral Fusion Proteins - immunology</topic><topic>Viral Proteins - immunology</topic><topic>Viral Vaccines - immunology</topic><topic>Virology</topic><topic>Virus Replication - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Werle, Bettina</creatorcontrib><creatorcontrib>Fromantin, Catherine</creatorcontrib><creatorcontrib>Alexandre, Anne</creatorcontrib><creatorcontrib>Kohli, Evelyne</creatorcontrib><creatorcontrib>Pothier, Pierre</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Werle, Bettina</au><au>Fromantin, Catherine</au><au>Alexandre, Anne</au><au>Kohli, Evelyne</au><au>Pothier, Pierre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dose-dependent effects of IL-12 treatment to immune response induced after immunization with a recombinant respiratory syncytial virus (RSV) fusion protein fragment</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>1999-08-06</date><risdate>1999</risdate><volume>17</volume><issue>23</issue><spage>2983</spage><epage>2990</epage><pages>2983-2990</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>The amino acid (aa) sequence 190–289 of the RSV fusion (F) glycoprotein expressed in insect cells (bF
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190–289 on immune response and protective efficacy. Low doses of IL-12 (10 ng) reduced IL-4 and IL-5 secretion (but did not affect IL-10 production) and decreased inflammatory signs whereas high doses of IL-12 had no effects. In addition, IL-12 treatment did not improve resistance to RSV replication. These results suggest that IL-12 treatment attenuates Th2 response and Th2 associated pulmonary inflammatory response in a dose-dependent manner, without improving protective efficacy.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>10462233</pmid><doi>10.1016/S0264-410X(99)00172-3</doi><tpages>8</tpages></addata></record> |
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| subjects | AIDS/HIV Animals Antigens, Viral - immunology Biological and medical sciences Bronchoalveolar Lavage Fluid - cytology Bronchoalveolar Lavage Fluid - immunology CD4-CD8 Ratio Dose-Response Relationship, Immunologic Eosinophils - cytology Female Fundamental and applied biological sciences. Psychology HN Protein IL-12 Immune response Interleukin-10 - biosynthesis Interleukin-12 - pharmacology Interleukin-4 - biosynthesis Interleukin-5 - biosynthesis Leukocyte Count Lymphocyte Activation - immunology Macrophages - cytology Mice Mice, Inbred BALB C Microbiology Neutralization Tests Peptide Fragments - immunology Respiratory syncytial virus Respiratory Syncytial Viruses - immunology Th2 Cells - immunology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Viral Envelope Proteins Viral Fusion Proteins - immunology Viral Proteins - immunology Viral Vaccines - immunology Virology Virus Replication - immunology |
| title | Dose-dependent effects of IL-12 treatment to immune response induced after immunization with a recombinant respiratory syncytial virus (RSV) fusion protein fragment |
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