In situ hybridization for telomerase RNA in routine cytologic brushings for the diagnosis of pancreaticobiliary malignancies
Background: Brush cytology for the diagnosis of pancreaticobiliary malignancy has an overall sensitivity of 50%. Accurate and specific markers are therefore needed for the optimal evaluation of pancreaticobiliary strictures. Telomerase activity is present in 85% to 90% of all human cancers. We sough...
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| Veröffentlicht in: | Gastrointestinal endoscopy 1998-10, Vol.48 (4), p.402-405 |
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| creator | Morales, Carmela P. Burdick, J.Steven Saboorian, M.Hossein Wright, Woodring E. Shay, Jerry W. |
| description | Background: Brush cytology for the diagnosis of pancreaticobiliary malignancy has an overall sensitivity of 50%. Accurate and specific markers are therefore needed for the optimal evaluation of pancreaticobiliary strictures. Telomerase activity is present in 85% to 90% of all human cancers. We sought to determine the utility of in situ hybridization for telomerase RNA in endoscopic brushings for the diagnosis of pancreaticobiliary malignancy.
Methods: Endoscopic brushings from 18 patients with pancreatic or biliary strictures were evaluated by routine cytology and in situ hybridization for telomerase RNA.
Results: Eight of 18 strictures were malignant. Cytology was positive in 5 patients, whereas telomerase RNA was positive in 6. All malignancies were diagnosed by either cytology or telomerase RNA; however, both studies were positive in only 3. There were no false-positive results by either technique.
Conclusion: The detection of telomerase RNA in endoscopic brushings may be an important adjunct to cytology for cancer diagnosis in pancreaticobiliary strictures. |
| doi_str_mv | 10.1016/S0016-5107(98)70011-2 |
| format | Article |
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Methods: Endoscopic brushings from 18 patients with pancreatic or biliary strictures were evaluated by routine cytology and in situ hybridization for telomerase RNA.
Results: Eight of 18 strictures were malignant. Cytology was positive in 5 patients, whereas telomerase RNA was positive in 6. All malignancies were diagnosed by either cytology or telomerase RNA; however, both studies were positive in only 3. There were no false-positive results by either technique.
Conclusion: The detection of telomerase RNA in endoscopic brushings may be an important adjunct to cytology for cancer diagnosis in pancreaticobiliary strictures.</description><identifier>ISSN: 0016-5107</identifier><identifier>EISSN: 1097-6779</identifier><identifier>DOI: 10.1016/S0016-5107(98)70011-2</identifier><identifier>PMID: 9786114</identifier><identifier>CODEN: GAENBQ</identifier><language>eng</language><publisher>New York, NY: Mosby, Inc</publisher><subject>Adult ; Aged ; Bile Duct Neoplasms - diagnosis ; Bile Ducts - enzymology ; Bile Ducts - pathology ; Biological and medical sciences ; Cholangiopancreatography, Endoscopic Retrograde ; Digestive system. Abdomen ; Endoscopy ; Female ; Humans ; In Situ Hybridization ; Investigative techniques, diagnostic techniques (general aspects) ; Male ; Medical sciences ; Middle Aged ; Pancreatic Ducts - enzymology ; Pancreatic Ducts - pathology ; Pancreatic Neoplasms - diagnosis ; RNA - analysis ; Telomerase - analysis ; Telomerase - genetics</subject><ispartof>Gastrointestinal endoscopy, 1998-10, Vol.48 (4), p.402-405</ispartof><rights>1998 American Society for Gastrointestinal Endoscopy</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-ea6d3b03d11298d583de354ff3277eadb981251f6c4dc357eb959bfae38c7bd73</citedby><cites>FETCH-LOGICAL-c455t-ea6d3b03d11298d583de354ff3277eadb981251f6c4dc357eb959bfae38c7bd73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0016-5107(98)70011-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2405178$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9786114$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Morales, Carmela P.</creatorcontrib><creatorcontrib>Burdick, J.Steven</creatorcontrib><creatorcontrib>Saboorian, M.Hossein</creatorcontrib><creatorcontrib>Wright, Woodring E.</creatorcontrib><creatorcontrib>Shay, Jerry W.</creatorcontrib><title>In situ hybridization for telomerase RNA in routine cytologic brushings for the diagnosis of pancreaticobiliary malignancies</title><title>Gastrointestinal endoscopy</title><addtitle>Gastrointest Endosc</addtitle><description>Background: Brush cytology for the diagnosis of pancreaticobiliary malignancy has an overall sensitivity of 50%. Accurate and specific markers are therefore needed for the optimal evaluation of pancreaticobiliary strictures. Telomerase activity is present in 85% to 90% of all human cancers. We sought to determine the utility of in situ hybridization for telomerase RNA in endoscopic brushings for the diagnosis of pancreaticobiliary malignancy.
Methods: Endoscopic brushings from 18 patients with pancreatic or biliary strictures were evaluated by routine cytology and in situ hybridization for telomerase RNA.
Results: Eight of 18 strictures were malignant. Cytology was positive in 5 patients, whereas telomerase RNA was positive in 6. All malignancies were diagnosed by either cytology or telomerase RNA; however, both studies were positive in only 3. There were no false-positive results by either technique.
Conclusion: The detection of telomerase RNA in endoscopic brushings may be an important adjunct to cytology for cancer diagnosis in pancreaticobiliary strictures.</description><subject>Adult</subject><subject>Aged</subject><subject>Bile Duct Neoplasms - diagnosis</subject><subject>Bile Ducts - enzymology</subject><subject>Bile Ducts - pathology</subject><subject>Biological and medical sciences</subject><subject>Cholangiopancreatography, Endoscopic Retrograde</subject><subject>Digestive system. Abdomen</subject><subject>Endoscopy</subject><subject>Female</subject><subject>Humans</subject><subject>In Situ Hybridization</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Pancreatic Ducts - enzymology</subject><subject>Pancreatic Ducts - pathology</subject><subject>Pancreatic Neoplasms - diagnosis</subject><subject>RNA - analysis</subject><subject>Telomerase - analysis</subject><subject>Telomerase - genetics</subject><issn>0016-5107</issn><issn>1097-6779</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2PFCEQhonRrOPqT9iEgzF6aIWmaeBkNhs_Ntlo4seZ0FA9U6YHRujeZIw_XmZnMlcvEKjnLYqHkCvO3nLG-3ffWV0byZl6bfQbVU-8aR-RFWdGNb1S5jFZnZGn5FkpvxhjuhX8glwYpXvOuxX5extpwXmhm_2QMeAfN2OKdEyZzjClLWRXgH77ck0x0pyWGSNQv5_TlNbo6ZCXssG4LsfEBmhAt46pYKFppDsXfYba0qcBJ3R5T7duwnWs9wjlOXkyuqnAi9N-SX5-_PDj5nNz9_XT7c31XeM7KecGXB_EwETgvDU6SC0CCNmNo2iVAhcGo3kr-dj7LnghFQxGmmF0ILRXQ1Dikrw69t3l9HuBMtstFg_T5CKkpdjeGC204hWUR9DnVEqG0e4ybuvYljN7sG4frNuDUmu0fbBu25q7Oj2wDFsI59RJc62_PNVd8W4a8-H_5Yy1HZNc6Yq9P2JQZdwjZFuqpughYAY_25DwP4P8A40ioXo</recordid><startdate>19981001</startdate><enddate>19981001</enddate><creator>Morales, Carmela P.</creator><creator>Burdick, J.Steven</creator><creator>Saboorian, M.Hossein</creator><creator>Wright, Woodring E.</creator><creator>Shay, Jerry W.</creator><general>Mosby, Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19981001</creationdate><title>In situ hybridization for telomerase RNA in routine cytologic brushings for the diagnosis of pancreaticobiliary malignancies</title><author>Morales, Carmela P. ; Burdick, J.Steven ; Saboorian, M.Hossein ; Wright, Woodring E. ; Shay, Jerry W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-ea6d3b03d11298d583de354ff3277eadb981251f6c4dc357eb959bfae38c7bd73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Bile Duct Neoplasms - diagnosis</topic><topic>Bile Ducts - enzymology</topic><topic>Bile Ducts - pathology</topic><topic>Biological and medical sciences</topic><topic>Cholangiopancreatography, Endoscopic Retrograde</topic><topic>Digestive system. Abdomen</topic><topic>Endoscopy</topic><topic>Female</topic><topic>Humans</topic><topic>In Situ Hybridization</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Pancreatic Ducts - enzymology</topic><topic>Pancreatic Ducts - pathology</topic><topic>Pancreatic Neoplasms - diagnosis</topic><topic>RNA - analysis</topic><topic>Telomerase - analysis</topic><topic>Telomerase - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Morales, Carmela P.</creatorcontrib><creatorcontrib>Burdick, J.Steven</creatorcontrib><creatorcontrib>Saboorian, M.Hossein</creatorcontrib><creatorcontrib>Wright, Woodring E.</creatorcontrib><creatorcontrib>Shay, Jerry W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gastrointestinal endoscopy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Morales, Carmela P.</au><au>Burdick, J.Steven</au><au>Saboorian, M.Hossein</au><au>Wright, Woodring E.</au><au>Shay, Jerry W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In situ hybridization for telomerase RNA in routine cytologic brushings for the diagnosis of pancreaticobiliary malignancies</atitle><jtitle>Gastrointestinal endoscopy</jtitle><addtitle>Gastrointest Endosc</addtitle><date>1998-10-01</date><risdate>1998</risdate><volume>48</volume><issue>4</issue><spage>402</spage><epage>405</epage><pages>402-405</pages><issn>0016-5107</issn><eissn>1097-6779</eissn><coden>GAENBQ</coden><abstract>Background: Brush cytology for the diagnosis of pancreaticobiliary malignancy has an overall sensitivity of 50%. Accurate and specific markers are therefore needed for the optimal evaluation of pancreaticobiliary strictures. Telomerase activity is present in 85% to 90% of all human cancers. We sought to determine the utility of in situ hybridization for telomerase RNA in endoscopic brushings for the diagnosis of pancreaticobiliary malignancy.
Methods: Endoscopic brushings from 18 patients with pancreatic or biliary strictures were evaluated by routine cytology and in situ hybridization for telomerase RNA.
Results: Eight of 18 strictures were malignant. Cytology was positive in 5 patients, whereas telomerase RNA was positive in 6. All malignancies were diagnosed by either cytology or telomerase RNA; however, both studies were positive in only 3. There were no false-positive results by either technique.
Conclusion: The detection of telomerase RNA in endoscopic brushings may be an important adjunct to cytology for cancer diagnosis in pancreaticobiliary strictures.</abstract><cop>New York, NY</cop><pub>Mosby, Inc</pub><pmid>9786114</pmid><doi>10.1016/S0016-5107(98)70011-2</doi><tpages>4</tpages></addata></record> |
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| subjects | Adult Aged Bile Duct Neoplasms - diagnosis Bile Ducts - enzymology Bile Ducts - pathology Biological and medical sciences Cholangiopancreatography, Endoscopic Retrograde Digestive system. Abdomen Endoscopy Female Humans In Situ Hybridization Investigative techniques, diagnostic techniques (general aspects) Male Medical sciences Middle Aged Pancreatic Ducts - enzymology Pancreatic Ducts - pathology Pancreatic Neoplasms - diagnosis RNA - analysis Telomerase - analysis Telomerase - genetics |
| title | In situ hybridization for telomerase RNA in routine cytologic brushings for the diagnosis of pancreaticobiliary malignancies |
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