Developmental Changes in Expression of Angiotensinogen mRNA in Rat Nephron Segments
We studied the localization of angiotensinogen mRNA in rat nephron segments and the differences in angiotensinogen mRNA levels between male Sprague-Dawley rats at 6 and 12wk of age using reverse transcription and polymerase chain reaction (RT-PCR). Each nephron segment of the rat kidney was microdis...
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| Veröffentlicht in: | Hypertension Research 1998, Vol.21(3), pp.155-161 |
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| container_title | Hypertension Research |
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| creator | Yamaguchi, Satoshi Tamura, Kouichi Nyui, Nobuo Hibi, Kiyoshi Ishigami, Tomoaki Kihara, Minoru Yabana, Machiko Sesoko, Shoji Ishii, Masao Umemura, Satoshi |
| description | We studied the localization of angiotensinogen mRNA in rat nephron segments and the differences in angiotensinogen mRNA levels between male Sprague-Dawley rats at 6 and 12wk of age using reverse transcription and polymerase chain reaction (RT-PCR). Each nephron segment of the rat kidney was microdissected. Total RNA was prepared and used in the following RT-PCR assay. The PCR products were size-fractionated by agarose gel electrophoresis, visualized with ethidium bromide staining, and identified by Southern blot analysis. The relative amounts of products were determined by densitometry. Strong bands corresponding to angiotensinogen mRNA were detected from proximal convoluted and straight tubules, and weaker bands were found in glomeruli. The signals in all tissues in 12-wk-old rats were weaker than those in 6-wk-old rats. Since local angiotensinogen is the unique substrate of the tissue renin-angiotensin system and exerts an autocrine-paracrine influence on renal function, the changes in tubular angiotensinogen may be related to physiological and morphological changes in the rat kidney during development. (Hypertens Res 1998; 21: 155-161) |
| doi_str_mv | 10.1291/hypres.21.155 |
| format | Article |
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Each nephron segment of the rat kidney was microdissected. Total RNA was prepared and used in the following RT-PCR assay. The PCR products were size-fractionated by agarose gel electrophoresis, visualized with ethidium bromide staining, and identified by Southern blot analysis. The relative amounts of products were determined by densitometry. Strong bands corresponding to angiotensinogen mRNA were detected from proximal convoluted and straight tubules, and weaker bands were found in glomeruli. The signals in all tissues in 12-wk-old rats were weaker than those in 6-wk-old rats. Since local angiotensinogen is the unique substrate of the tissue renin-angiotensin system and exerts an autocrine-paracrine influence on renal function, the changes in tubular angiotensinogen may be related to physiological and morphological changes in the rat kidney during development. (Hypertens Res 1998; 21: 155-161)</description><identifier>ISSN: 0916-9636</identifier><identifier>EISSN: 1348-4214</identifier><identifier>DOI: 10.1291/hypres.21.155</identifier><identifier>PMID: 9786598</identifier><language>eng</language><publisher>England: The Japanese Society of Hypertension</publisher><subject>angiotensinogen ; Angiotensinogen - biosynthesis ; Animals ; Autoradiography ; Kidney Tubules, Proximal - drug effects ; Kidney Tubules, Proximal - physiology ; Male ; mRNA ; Nephrons - growth & development ; Nephrons - metabolism ; polymerase chain reaction ; Rats ; Rats, Sprague-Dawley ; renal tubule ; Reverse Transcriptase Polymerase Chain Reaction ; reverse transcription ; RNA, Messenger - biosynthesis</subject><ispartof>Hypertension Research, 1998, Vol.21(3), pp.155-161</ispartof><rights>The Japanese Society of Hypertension</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9786598$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamaguchi, Satoshi</creatorcontrib><creatorcontrib>Tamura, Kouichi</creatorcontrib><creatorcontrib>Nyui, Nobuo</creatorcontrib><creatorcontrib>Hibi, Kiyoshi</creatorcontrib><creatorcontrib>Ishigami, Tomoaki</creatorcontrib><creatorcontrib>Kihara, Minoru</creatorcontrib><creatorcontrib>Yabana, Machiko</creatorcontrib><creatorcontrib>Sesoko, Shoji</creatorcontrib><creatorcontrib>Ishii, Masao</creatorcontrib><creatorcontrib>Umemura, Satoshi</creatorcontrib><title>Developmental Changes in Expression of Angiotensinogen mRNA in Rat Nephron Segments</title><title>Hypertension Research</title><addtitle>Hypertension Research</addtitle><description>We studied the localization of angiotensinogen mRNA in rat nephron segments and the differences in angiotensinogen mRNA levels between male Sprague-Dawley rats at 6 and 12wk of age using reverse transcription and polymerase chain reaction (RT-PCR). Each nephron segment of the rat kidney was microdissected. Total RNA was prepared and used in the following RT-PCR assay. The PCR products were size-fractionated by agarose gel electrophoresis, visualized with ethidium bromide staining, and identified by Southern blot analysis. The relative amounts of products were determined by densitometry. Strong bands corresponding to angiotensinogen mRNA were detected from proximal convoluted and straight tubules, and weaker bands were found in glomeruli. The signals in all tissues in 12-wk-old rats were weaker than those in 6-wk-old rats. Since local angiotensinogen is the unique substrate of the tissue renin-angiotensin system and exerts an autocrine-paracrine influence on renal function, the changes in tubular angiotensinogen may be related to physiological and morphological changes in the rat kidney during development. (Hypertens Res 1998; 21: 155-161)</description><subject>angiotensinogen</subject><subject>Angiotensinogen - biosynthesis</subject><subject>Animals</subject><subject>Autoradiography</subject><subject>Kidney Tubules, Proximal - drug effects</subject><subject>Kidney Tubules, Proximal - physiology</subject><subject>Male</subject><subject>mRNA</subject><subject>Nephrons - growth & development</subject><subject>Nephrons - metabolism</subject><subject>polymerase chain reaction</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>renal tubule</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>reverse transcription</subject><subject>RNA, Messenger - biosynthesis</subject><issn>0916-9636</issn><issn>1348-4214</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1LwzAYh4Moc06PHoWevHUmTdIlxzE3FcaETc8hS99-jDatSSfuv7elY5e8gd_Dc3gQeiR4SiJJXvJT48BPIzIlnF-hMaFMhCwi7BqNsSRxKGMa36I77w8YR4JLMkIjORMxl2KMdq_wC2XdVGBbXQaLXNsMfFDYYPnXe31R26BOg7nNiroF6wtbZ2CDaruZ99RWt8EGmtx12A6yXuPv0U2qSw8P5ztB36vl1-I9XH--fSzm69BwjNuQamO0NBKihLA0YSmdUcM0cBwTQRjhghohU86TdJYCZVyzfdL_4wTiPZZ0gp4Hb-PqnyP4VlWFN1CW2kJ99CqWUnQdcAeGA2hc7b2DVDWuqLQ7KYJVH1ENEVVEVBex45_O4uO-guRCn6t1-2rYD77VGVx27drClHC2ESmj3kiHpxNfAJNrp8DSf-gZiNM</recordid><startdate>1998</startdate><enddate>1998</enddate><creator>Yamaguchi, Satoshi</creator><creator>Tamura, Kouichi</creator><creator>Nyui, Nobuo</creator><creator>Hibi, Kiyoshi</creator><creator>Ishigami, Tomoaki</creator><creator>Kihara, Minoru</creator><creator>Yabana, Machiko</creator><creator>Sesoko, Shoji</creator><creator>Ishii, Masao</creator><creator>Umemura, Satoshi</creator><general>The Japanese Society of Hypertension</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1998</creationdate><title>Developmental Changes in Expression of Angiotensinogen mRNA in Rat Nephron Segments</title><author>Yamaguchi, Satoshi ; Tamura, Kouichi ; Nyui, Nobuo ; Hibi, Kiyoshi ; Ishigami, Tomoaki ; Kihara, Minoru ; Yabana, Machiko ; Sesoko, Shoji ; Ishii, Masao ; Umemura, Satoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c500t-3acca9c9e2d14fd4f373c4ae50618141583c89f55df7fe345a4bddf7f6de6b093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>angiotensinogen</topic><topic>Angiotensinogen - biosynthesis</topic><topic>Animals</topic><topic>Autoradiography</topic><topic>Kidney Tubules, Proximal - drug effects</topic><topic>Kidney Tubules, Proximal - physiology</topic><topic>Male</topic><topic>mRNA</topic><topic>Nephrons - growth & development</topic><topic>Nephrons - metabolism</topic><topic>polymerase chain reaction</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>renal tubule</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>reverse transcription</topic><topic>RNA, Messenger - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamaguchi, Satoshi</creatorcontrib><creatorcontrib>Tamura, Kouichi</creatorcontrib><creatorcontrib>Nyui, Nobuo</creatorcontrib><creatorcontrib>Hibi, Kiyoshi</creatorcontrib><creatorcontrib>Ishigami, Tomoaki</creatorcontrib><creatorcontrib>Kihara, Minoru</creatorcontrib><creatorcontrib>Yabana, Machiko</creatorcontrib><creatorcontrib>Sesoko, Shoji</creatorcontrib><creatorcontrib>Ishii, Masao</creatorcontrib><creatorcontrib>Umemura, Satoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Hypertension Research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamaguchi, Satoshi</au><au>Tamura, Kouichi</au><au>Nyui, Nobuo</au><au>Hibi, Kiyoshi</au><au>Ishigami, Tomoaki</au><au>Kihara, Minoru</au><au>Yabana, Machiko</au><au>Sesoko, Shoji</au><au>Ishii, Masao</au><au>Umemura, Satoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Developmental Changes in Expression of Angiotensinogen mRNA in Rat Nephron Segments</atitle><jtitle>Hypertension Research</jtitle><addtitle>Hypertension Research</addtitle><date>1998</date><risdate>1998</risdate><volume>21</volume><issue>3</issue><spage>155</spage><epage>161</epage><pages>155-161</pages><issn>0916-9636</issn><eissn>1348-4214</eissn><abstract>We studied the localization of angiotensinogen mRNA in rat nephron segments and the differences in angiotensinogen mRNA levels between male Sprague-Dawley rats at 6 and 12wk of age using reverse transcription and polymerase chain reaction (RT-PCR). Each nephron segment of the rat kidney was microdissected. Total RNA was prepared and used in the following RT-PCR assay. The PCR products were size-fractionated by agarose gel electrophoresis, visualized with ethidium bromide staining, and identified by Southern blot analysis. The relative amounts of products were determined by densitometry. Strong bands corresponding to angiotensinogen mRNA were detected from proximal convoluted and straight tubules, and weaker bands were found in glomeruli. The signals in all tissues in 12-wk-old rats were weaker than those in 6-wk-old rats. Since local angiotensinogen is the unique substrate of the tissue renin-angiotensin system and exerts an autocrine-paracrine influence on renal function, the changes in tubular angiotensinogen may be related to physiological and morphological changes in the rat kidney during development. (Hypertens Res 1998; 21: 155-161)</abstract><cop>England</cop><pub>The Japanese Society of Hypertension</pub><pmid>9786598</pmid><doi>10.1291/hypres.21.155</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese |
| subjects | angiotensinogen Angiotensinogen - biosynthesis Animals Autoradiography Kidney Tubules, Proximal - drug effects Kidney Tubules, Proximal - physiology Male mRNA Nephrons - growth & development Nephrons - metabolism polymerase chain reaction Rats Rats, Sprague-Dawley renal tubule Reverse Transcriptase Polymerase Chain Reaction reverse transcription RNA, Messenger - biosynthesis |
| title | Developmental Changes in Expression of Angiotensinogen mRNA in Rat Nephron Segments |
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