Two peptidergic drugs increase the synaptophysin immunoreactivity in brains of 24-month-old rats
The brain-derived peptidergic drug Cerebrolysin has been found to support the survival of neurones in vitro and in vivo. Positive effects on learning and memory have been demonstrated in various animal models and also in clinical trails. In the present study, the effects of Cerebrolysin and its pept...
Gespeichert in:
| Veröffentlicht in: | Journal of molecular histology 1999-06, Vol.31 (6), p.395-401 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 401 |
|---|---|
| container_issue | 6 |
| container_start_page | 395 |
| container_title | Journal of molecular histology |
| container_volume | 31 |
| creator | Reinprecht, I Gschanes, A Windisch, M Fachbach, G |
| description | The brain-derived peptidergic drug Cerebrolysin has been found to support the survival of neurones in vitro and in vivo. Positive effects on learning and memory have been demonstrated in various animal models and also in clinical trails. In the present study, the effects of Cerebrolysin and its peptide preparation E021 on the synapse density in the hippocampus, the dentate gyrus and in the entorhinal cortex of 24-month-old rats were investigated. Rats received the drugs or saline for control for 19 consecutive days (2.5 ml/kg per day). Slices of the brains were immunohistochemically stained with anti-synaptophysin, which is a specific marker of presynaptic terminals. Quantification of the synapse density was done by using light microscopy and a computerised image analysing system. Our results clearly showed that the rats benefit from the administration of both drugs, showing an enhancement in the number of synaptophysin-immunostained presynaptic terminals in the entorhinal cortex, the dentate gyrus, and also in the hippocampal subfields CA1, CA2, CA3 stratum lucidum and CA3 stratum radiatum. It can be assumed that these effects are the reason for improved cognitive performances of rats treated with Cerebrolysin and E021. |
| doi_str_mv | 10.1023/A:1003752208971 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_69982096</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69982096</sourcerecordid><originalsourceid>FETCH-LOGICAL-c196t-c24e0d0819274928edf9d11788da7dac3c2404429566a760bcb9d6436b50bef83</originalsourceid><addsrcrecordid>eNpd0M9LwzAUwPEgipvTszcJCN6qL2mbH95k-AsGXua5pk26ZaxNTVKl_70B58XTO7wPj8cXoUsCtwRofvdwTwByXlIKQnJyhOakZDyjueDHaA5AREYpKWboLIQdAEjO2SmaESgYpbSco4_1t8ODGaLVxm9sg7UfNwHbvvFGBYPj1uAw9WqIbthOwfbYdt3Yu7Rtov2ycUoW117ZPmDXYlpknevjNnN7jb2K4RydtGofzMVhLtD70-N6-ZKt3p5flw-rrCGSxayhhQENgkjKC0mF0a3UhHAhtOJaNXkCUBRUlowpzqBuaqlZkbO6hNq0Il-gm9-7g3efowmx6mxozH6veuPGUDEpBQXJErz-B3du9H36rUpJU0whcpLU1UGNdWd0NXjbKT9Vf-XyHyhEb9A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1020378831</pqid></control><display><type>article</type><title>Two peptidergic drugs increase the synaptophysin immunoreactivity in brains of 24-month-old rats</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Reinprecht, I ; Gschanes, A ; Windisch, M ; Fachbach, G</creator><creatorcontrib>Reinprecht, I ; Gschanes, A ; Windisch, M ; Fachbach, G</creatorcontrib><description>The brain-derived peptidergic drug Cerebrolysin has been found to support the survival of neurones in vitro and in vivo. Positive effects on learning and memory have been demonstrated in various animal models and also in clinical trails. In the present study, the effects of Cerebrolysin and its peptide preparation E021 on the synapse density in the hippocampus, the dentate gyrus and in the entorhinal cortex of 24-month-old rats were investigated. Rats received the drugs or saline for control for 19 consecutive days (2.5 ml/kg per day). Slices of the brains were immunohistochemically stained with anti-synaptophysin, which is a specific marker of presynaptic terminals. Quantification of the synapse density was done by using light microscopy and a computerised image analysing system. Our results clearly showed that the rats benefit from the administration of both drugs, showing an enhancement in the number of synaptophysin-immunostained presynaptic terminals in the entorhinal cortex, the dentate gyrus, and also in the hippocampal subfields CA1, CA2, CA3 stratum lucidum and CA3 stratum radiatum. It can be assumed that these effects are the reason for improved cognitive performances of rats treated with Cerebrolysin and E021.</description><identifier>ISSN: 0018-2214</identifier><identifier>ISSN: 1567-2379</identifier><identifier>EISSN: 1567-2387</identifier><identifier>DOI: 10.1023/A:1003752208971</identifier><identifier>PMID: 10462225</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Amino Acids - pharmacology ; Animals ; Brain - anatomy & histology ; Brain - drug effects ; Brain - metabolism ; Cellular biology ; Image Processing, Computer-Assisted - methods ; Immunohistochemistry - methods ; Microscopy - methods ; Neurons ; Neuropeptides - pharmacology ; Nootropic Agents - pharmacology ; Presynaptic Terminals - drug effects ; Presynaptic Terminals - metabolism ; Rats ; Rodents ; Synaptophysin - immunology</subject><ispartof>Journal of molecular histology, 1999-06, Vol.31 (6), p.395-401</ispartof><rights>Kluwer Academic Publishers 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c196t-c24e0d0819274928edf9d11788da7dac3c2404429566a760bcb9d6436b50bef83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10462225$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reinprecht, I</creatorcontrib><creatorcontrib>Gschanes, A</creatorcontrib><creatorcontrib>Windisch, M</creatorcontrib><creatorcontrib>Fachbach, G</creatorcontrib><title>Two peptidergic drugs increase the synaptophysin immunoreactivity in brains of 24-month-old rats</title><title>Journal of molecular histology</title><addtitle>Histochem J</addtitle><description>The brain-derived peptidergic drug Cerebrolysin has been found to support the survival of neurones in vitro and in vivo. Positive effects on learning and memory have been demonstrated in various animal models and also in clinical trails. In the present study, the effects of Cerebrolysin and its peptide preparation E021 on the synapse density in the hippocampus, the dentate gyrus and in the entorhinal cortex of 24-month-old rats were investigated. Rats received the drugs or saline for control for 19 consecutive days (2.5 ml/kg per day). Slices of the brains were immunohistochemically stained with anti-synaptophysin, which is a specific marker of presynaptic terminals. Quantification of the synapse density was done by using light microscopy and a computerised image analysing system. Our results clearly showed that the rats benefit from the administration of both drugs, showing an enhancement in the number of synaptophysin-immunostained presynaptic terminals in the entorhinal cortex, the dentate gyrus, and also in the hippocampal subfields CA1, CA2, CA3 stratum lucidum and CA3 stratum radiatum. It can be assumed that these effects are the reason for improved cognitive performances of rats treated with Cerebrolysin and E021.</description><subject>Amino Acids - pharmacology</subject><subject>Animals</subject><subject>Brain - anatomy & histology</subject><subject>Brain - drug effects</subject><subject>Brain - metabolism</subject><subject>Cellular biology</subject><subject>Image Processing, Computer-Assisted - methods</subject><subject>Immunohistochemistry - methods</subject><subject>Microscopy - methods</subject><subject>Neurons</subject><subject>Neuropeptides - pharmacology</subject><subject>Nootropic Agents - pharmacology</subject><subject>Presynaptic Terminals - drug effects</subject><subject>Presynaptic Terminals - metabolism</subject><subject>Rats</subject><subject>Rodents</subject><subject>Synaptophysin - immunology</subject><issn>0018-2214</issn><issn>1567-2379</issn><issn>1567-2387</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpd0M9LwzAUwPEgipvTszcJCN6qL2mbH95k-AsGXua5pk26ZaxNTVKl_70B58XTO7wPj8cXoUsCtwRofvdwTwByXlIKQnJyhOakZDyjueDHaA5AREYpKWboLIQdAEjO2SmaESgYpbSco4_1t8ODGaLVxm9sg7UfNwHbvvFGBYPj1uAw9WqIbthOwfbYdt3Yu7Rtov2ycUoW117ZPmDXYlpknevjNnN7jb2K4RydtGofzMVhLtD70-N6-ZKt3p5flw-rrCGSxayhhQENgkjKC0mF0a3UhHAhtOJaNXkCUBRUlowpzqBuaqlZkbO6hNq0Il-gm9-7g3efowmx6mxozH6veuPGUDEpBQXJErz-B3du9H36rUpJU0whcpLU1UGNdWd0NXjbKT9Vf-XyHyhEb9A</recordid><startdate>199906</startdate><enddate>199906</enddate><creator>Reinprecht, I</creator><creator>Gschanes, A</creator><creator>Windisch, M</creator><creator>Fachbach, G</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>199906</creationdate><title>Two peptidergic drugs increase the synaptophysin immunoreactivity in brains of 24-month-old rats</title><author>Reinprecht, I ; Gschanes, A ; Windisch, M ; Fachbach, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c196t-c24e0d0819274928edf9d11788da7dac3c2404429566a760bcb9d6436b50bef83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acids - pharmacology</topic><topic>Animals</topic><topic>Brain - anatomy & histology</topic><topic>Brain - drug effects</topic><topic>Brain - metabolism</topic><topic>Cellular biology</topic><topic>Image Processing, Computer-Assisted - methods</topic><topic>Immunohistochemistry - methods</topic><topic>Microscopy - methods</topic><topic>Neurons</topic><topic>Neuropeptides - pharmacology</topic><topic>Nootropic Agents - pharmacology</topic><topic>Presynaptic Terminals - drug effects</topic><topic>Presynaptic Terminals - metabolism</topic><topic>Rats</topic><topic>Rodents</topic><topic>Synaptophysin - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reinprecht, I</creatorcontrib><creatorcontrib>Gschanes, A</creatorcontrib><creatorcontrib>Windisch, M</creatorcontrib><creatorcontrib>Fachbach, G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular histology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reinprecht, I</au><au>Gschanes, A</au><au>Windisch, M</au><au>Fachbach, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Two peptidergic drugs increase the synaptophysin immunoreactivity in brains of 24-month-old rats</atitle><jtitle>Journal of molecular histology</jtitle><addtitle>Histochem J</addtitle><date>1999-06</date><risdate>1999</risdate><volume>31</volume><issue>6</issue><spage>395</spage><epage>401</epage><pages>395-401</pages><issn>0018-2214</issn><issn>1567-2379</issn><eissn>1567-2387</eissn><abstract>The brain-derived peptidergic drug Cerebrolysin has been found to support the survival of neurones in vitro and in vivo. Positive effects on learning and memory have been demonstrated in various animal models and also in clinical trails. In the present study, the effects of Cerebrolysin and its peptide preparation E021 on the synapse density in the hippocampus, the dentate gyrus and in the entorhinal cortex of 24-month-old rats were investigated. Rats received the drugs or saline for control for 19 consecutive days (2.5 ml/kg per day). Slices of the brains were immunohistochemically stained with anti-synaptophysin, which is a specific marker of presynaptic terminals. Quantification of the synapse density was done by using light microscopy and a computerised image analysing system. Our results clearly showed that the rats benefit from the administration of both drugs, showing an enhancement in the number of synaptophysin-immunostained presynaptic terminals in the entorhinal cortex, the dentate gyrus, and also in the hippocampal subfields CA1, CA2, CA3 stratum lucidum and CA3 stratum radiatum. It can be assumed that these effects are the reason for improved cognitive performances of rats treated with Cerebrolysin and E021.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>10462225</pmid><doi>10.1023/A:1003752208971</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0018-2214 |
| ispartof | Journal of molecular histology, 1999-06, Vol.31 (6), p.395-401 |
| issn | 0018-2214 1567-2379 1567-2387 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69982096 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Amino Acids - pharmacology Animals Brain - anatomy & histology Brain - drug effects Brain - metabolism Cellular biology Image Processing, Computer-Assisted - methods Immunohistochemistry - methods Microscopy - methods Neurons Neuropeptides - pharmacology Nootropic Agents - pharmacology Presynaptic Terminals - drug effects Presynaptic Terminals - metabolism Rats Rodents Synaptophysin - immunology |
| title | Two peptidergic drugs increase the synaptophysin immunoreactivity in brains of 24-month-old rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T02%3A54%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Two%20peptidergic%20drugs%20increase%20the%20synaptophysin%20immunoreactivity%20in%20brains%20of%2024-month-old%20rats&rft.jtitle=Journal%20of%20molecular%20histology&rft.au=Reinprecht,%20I&rft.date=1999-06&rft.volume=31&rft.issue=6&rft.spage=395&rft.epage=401&rft.pages=395-401&rft.issn=0018-2214&rft.eissn=1567-2387&rft_id=info:doi/10.1023/A:1003752208971&rft_dat=%3Cproquest_pubme%3E69982096%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1020378831&rft_id=info:pmid/10462225&rfr_iscdi=true |