A colorimetric microtiter plate PCR system detects respiratory syncytial virus in nasal aspirates and discriminates subtypes A and B

We developed a colorimetric microtiter plate (MTP) PCR system for specific detection of the respiratory syncytial virus (RSV) nucleocapsid gene and differentiation of viral subtypes A and B. Of 47 pediatric nasal aspirate specimens, the sensitivity and specificity were 94.4% (17 of 18) and 100% (15...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 1999-08, Vol.34 (4), p.333-337
Hauptverfasser:	Tang, Yi-Wei, Heimgartner, Paul J, Tollefson, Sharon J, Berg, Timothy J, Rys, Paul N, Li, Haijing, Smith, Thomas F, Persing, David H, Wright, Peter F
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Cell Culture Techniques - methods Child Child, Preschool Colorimetry - methods Efficiency Enzyme-Linked Immunosorbent Assay - methods Fundamental and applied biological sciences. Psychology Human viral diseases Humans Infant Infant, Newborn Infectious diseases Medical sciences Microbiology Nasal Lavage Fluid - virology Respiratory Syncytial Virus Infections - diagnosis Respiratory Syncytial Viruses - classification Respiratory Syncytial Viruses - isolation & purification Reverse Transcriptase Polymerase Chain Reaction - methods Reverse Transcriptase Polymerase Chain Reaction - standards Sensitivity and Specificity Techniques used in virology Viral diseases Viral diseases of the respiratory system and ent viral diseases Virology
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