Utilization of Antisense Oligodeoxynucleotides with Embryonic Tissues in Culture
Experimental embryology has long used manipulation of interacting tissues to examine questions of tissue interaction and differentiation. The potential for specific manipulation of gene expression in such tissues has made the utilization of antisense techniques desirable. However, problems with this...
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| Veröffentlicht in: | Methods (San Diego, Calif.) Calif.), 1999-07, Vol.18 (3), p.316-321 |
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| container_title | Methods (San Diego, Calif.) |
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| creator | Runyan, Raymond B. Wendler, Christopher C. Romano, Laura A. Boyer, Angelique S. Dagle, John M. Weeks, Daniel L. |
| description | Experimental embryology has long used manipulation of interacting tissues to examine questions of tissue interaction and differentiation. The potential for specific manipulation of gene expression in such tissues has made the utilization of antisense techniques desirable. However, problems with this methodology have discouraged many investigators from using this approach. Selection of target sequences for antisense oligonucleotides, delivery of oligonucleotides into cells or tissues, and the type of modification of the oligonucleotide to be used all present concerns that must be addressed. This paper describes our approach to selection of target sequence and methods of delivery and describes the synthesis of a methoxyethylamidate-modified antisense oligonucleotide that has proved useful in our studies. This approach has enabled us to explore aspects of tissue interaction in the embryonic heart that would have been difficult to explore in a genetic model. |
| doi_str_mv | 10.1006/meth.1999.0790 |
| format | Article |
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This approach has enabled us to explore aspects of tissue interaction in the embryonic heart that would have been difficult to explore in a genetic model.</description><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Gene Targeting - methods</subject><subject>Heart - embryology</subject><subject>Liposomes - chemistry</subject><subject>Nucleic Acid Hybridization</subject><subject>Oligodeoxyribonucleotides, Antisense - chemical synthesis</subject><subject>Oligodeoxyribonucleotides, Antisense - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>Transforming Growth Factor beta - genetics</subject><issn>1046-2023</issn><issn>1095-9130</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kD1PwzAQhi0EoqWwMqJMbAl2nDjxWFXlQ0IqQ5mtxLlQo8QutgOUX4-jdGBh8tl-7tXdg9A1wQnBmN314HcJ4ZwnuOD4BM0J5nnMCcWnY52xOMUpnaEL594xxiQtynM0Cx95xjmZo5dXrzr1U3lldGTaaKm9cqAdRJtOvZkGzPdBD7ID41UDLvpSfhet-9oejFYy2irnhvCsdLQaOj9YuERnbdU5uDqeC7S9X29Xj_Hz5uFptXyOJc2wj9sCCskkZKXkNS1IuDRlntKKtDSjRZkzyQoJkkOVsxSHRXhec1xDU_KMlnSBbqfYvTUfYQIveuUkdF2lwQxOMM5LzFgWwGQCpTXOWWjF3qq-sgdBsBgVilGhGBWKUWFouDkmD3UPzR98chaAcgIgrPepwAonFWgJjbIgvWiM-i_7F2KWgSs</recordid><startdate>19990701</startdate><enddate>19990701</enddate><creator>Runyan, Raymond B.</creator><creator>Wendler, Christopher C.</creator><creator>Romano, Laura A.</creator><creator>Boyer, Angelique S.</creator><creator>Dagle, John M.</creator><creator>Weeks, Daniel L.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990701</creationdate><title>Utilization of Antisense Oligodeoxynucleotides with Embryonic Tissues in Culture</title><author>Runyan, Raymond B. ; Wendler, Christopher C. ; Romano, Laura A. ; Boyer, Angelique S. ; Dagle, John M. ; Weeks, Daniel L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-f7e7c6ce48c9b3717c6d8523a1f3437856c67cec9ea562004695b90bed894383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Gene Targeting - methods</topic><topic>Heart - embryology</topic><topic>Liposomes - chemistry</topic><topic>Nucleic Acid Hybridization</topic><topic>Oligodeoxyribonucleotides, Antisense - chemical synthesis</topic><topic>Oligodeoxyribonucleotides, Antisense - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>Transforming Growth Factor beta - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Runyan, Raymond B.</creatorcontrib><creatorcontrib>Wendler, Christopher C.</creatorcontrib><creatorcontrib>Romano, Laura A.</creatorcontrib><creatorcontrib>Boyer, Angelique S.</creatorcontrib><creatorcontrib>Dagle, John M.</creatorcontrib><creatorcontrib>Weeks, Daniel L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Methods (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Runyan, Raymond B.</au><au>Wendler, Christopher C.</au><au>Romano, Laura A.</au><au>Boyer, Angelique S.</au><au>Dagle, John M.</au><au>Weeks, Daniel L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Utilization of Antisense Oligodeoxynucleotides with Embryonic Tissues in Culture</atitle><jtitle>Methods (San Diego, Calif.)</jtitle><addtitle>Methods</addtitle><date>1999-07-01</date><risdate>1999</risdate><volume>18</volume><issue>3</issue><spage>316</spage><epage>321</epage><pages>316-321</pages><issn>1046-2023</issn><eissn>1095-9130</eissn><abstract>Experimental embryology has long used manipulation of interacting tissues to examine questions of tissue interaction and differentiation. 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| ispartof | Methods (San Diego, Calif.), 1999-07, Vol.18 (3), p.316-321 |
| issn | 1046-2023 1095-9130 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Cells, Cultured Gene Targeting - methods Heart - embryology Liposomes - chemistry Nucleic Acid Hybridization Oligodeoxyribonucleotides, Antisense - chemical synthesis Oligodeoxyribonucleotides, Antisense - genetics RNA, Messenger - genetics Transforming Growth Factor beta - genetics |
| title | Utilization of Antisense Oligodeoxynucleotides with Embryonic Tissues in Culture |
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