Vibrio cholerae iron transport: haem transport genes are linked to one of two sets of tonB, exbB, exbD genes
Vibrio cholerae was found to have two sets of genes encoding TonB, ExbB and ExbD proteins. The first set (tonB1, exbB1, exbD1) was obtained by complementation of a V. cholerae tonB mutant. In the mutant, a plasmid containing these genes permitted transport via the known V. cholerae high‐affinity iro...
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description | Vibrio cholerae was found to have two sets of genes encoding TonB, ExbB and ExbD proteins. The first set (tonB1, exbB1, exbD1) was obtained by complementation of a V. cholerae tonB mutant. In the mutant, a plasmid containing these genes permitted transport via the known V. cholerae high‐affinity iron transport systems, including uptake of haem, vibriobactin and ferrichrome. When chromosomal mutations in exbB1 or exbD1 were introduced into a wild‐type V. cholerae background, no defect in iron transport was noted, indicating the existence of additional genes that can complement the defect in the wild‐type background. Another region of the V. cholerae chromosome was cloned that encoded a second functional TonB/Exb system (tonB2, exbB2, exbD2). A chromosomal mutation in exbB2 also failed to exhibit a defect in iron transport, but a V. cholerae strain that had chromosomal mutations in both the exbB1 and exbB2 genes displayed a mutant phenotype similar to that of an Escherichia coli tonB mutant. The genes encoding TonB1, ExbB1, ExbD1 were part of an operon that included three haem transport genes (hutBCD), and all six genes appeared to be expressed from a single Fur‐regulated promoter upstream of tonB1. A plasmid containing all six genes permitted utilization of haem by an E. coli strain expressing the V. cholerae haem receptor, HutA. Analysis of the hut genes indicated that hutBCD, which are predicted to encode a periplasmic binding protein (HutB) and cytoplasmic membrane permease (HutC and HutD), were required to reconstitute the V. cholerae haem transport system in E. coli. In V. cholerae, the presence of hutBCD stimulated growth when haemin was the iron source, but these genes were not essential for haemin utilization in V. cholerae. |
doi_str_mv | 10.1046/j.1365-2958.1998.01034.x |
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The first set (tonB1, exbB1, exbD1) was obtained by complementation of a V. cholerae tonB mutant. In the mutant, a plasmid containing these genes permitted transport via the known V. cholerae high‐affinity iron transport systems, including uptake of haem, vibriobactin and ferrichrome. When chromosomal mutations in exbB1 or exbD1 were introduced into a wild‐type V. cholerae background, no defect in iron transport was noted, indicating the existence of additional genes that can complement the defect in the wild‐type background. Another region of the V. cholerae chromosome was cloned that encoded a second functional TonB/Exb system (tonB2, exbB2, exbD2). A chromosomal mutation in exbB2 also failed to exhibit a defect in iron transport, but a V. cholerae strain that had chromosomal mutations in both the exbB1 and exbB2 genes displayed a mutant phenotype similar to that of an Escherichia coli tonB mutant. The genes encoding TonB1, ExbB1, ExbD1 were part of an operon that included three haem transport genes (hutBCD), and all six genes appeared to be expressed from a single Fur‐regulated promoter upstream of tonB1. A plasmid containing all six genes permitted utilization of haem by an E. coli strain expressing the V. cholerae haem receptor, HutA. Analysis of the hut genes indicated that hutBCD, which are predicted to encode a periplasmic binding protein (HutB) and cytoplasmic membrane permease (HutC and HutD), were required to reconstitute the V. cholerae haem transport system in E. coli. In V. cholerae, the presence of hutBCD stimulated growth when haemin was the iron source, but these genes were not essential for haemin utilization in V. cholerae.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1046/j.1365-2958.1998.01034.x</identifier><identifier>PMID: 9781885</identifier><language>eng</language><publisher>Oxford BSL: Blackwell Science Ltd, UK</publisher><subject>Amino Acid Sequence ; Bacterial Proteins - genetics ; Base Sequence ; Biological Transport, Active - genetics ; Cloning, Molecular ; DNA Primers - genetics ; DNA, Bacterial - genetics ; Escherichia coli ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Escherichia coli Proteins ; Genes, Bacterial ; Genetic Complementation Test ; Genetic Linkage ; Heme - metabolism ; Iron - metabolism ; Membrane Proteins - genetics ; Molecular Sequence Data ; Mutation ; Restriction Mapping ; Species Specificity ; Vibrio cholerae ; Vibrio cholerae - genetics ; Vibrio cholerae - metabolism</subject><ispartof>Molecular microbiology, 1998-09, Vol.29 (6), p.1493-1507</ispartof><rights>Blackwell Science Ltd, Oxford</rights><rights>Copyright Blackwell Scientific Publications Ltd. Sep 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4884-5d4485d1bb72f2202b2444a1856d207cbc29e2fe695a7a895fc9da7f25a629973</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2958.1998.01034.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2958.1998.01034.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9781885$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Occhino, Deborah A.</creatorcontrib><creatorcontrib>Wyckoff, Elizabeth E.</creatorcontrib><creatorcontrib>Henderson, Douglas P.</creatorcontrib><creatorcontrib>Wrona, Thomas J.</creatorcontrib><creatorcontrib>Payne, Shelley M.</creatorcontrib><title>Vibrio cholerae iron transport: haem transport genes are linked to one of two sets of tonB, exbB, exbD genes</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Vibrio cholerae was found to have two sets of genes encoding TonB, ExbB and ExbD proteins. The first set (tonB1, exbB1, exbD1) was obtained by complementation of a V. cholerae tonB mutant. In the mutant, a plasmid containing these genes permitted transport via the known V. cholerae high‐affinity iron transport systems, including uptake of haem, vibriobactin and ferrichrome. When chromosomal mutations in exbB1 or exbD1 were introduced into a wild‐type V. cholerae background, no defect in iron transport was noted, indicating the existence of additional genes that can complement the defect in the wild‐type background. Another region of the V. cholerae chromosome was cloned that encoded a second functional TonB/Exb system (tonB2, exbB2, exbD2). A chromosomal mutation in exbB2 also failed to exhibit a defect in iron transport, but a V. cholerae strain that had chromosomal mutations in both the exbB1 and exbB2 genes displayed a mutant phenotype similar to that of an Escherichia coli tonB mutant. The genes encoding TonB1, ExbB1, ExbD1 were part of an operon that included three haem transport genes (hutBCD), and all six genes appeared to be expressed from a single Fur‐regulated promoter upstream of tonB1. A plasmid containing all six genes permitted utilization of haem by an E. coli strain expressing the V. cholerae haem receptor, HutA. Analysis of the hut genes indicated that hutBCD, which are predicted to encode a periplasmic binding protein (HutB) and cytoplasmic membrane permease (HutC and HutD), were required to reconstitute the V. cholerae haem transport system in E. coli. In V. cholerae, the presence of hutBCD stimulated growth when haemin was the iron source, but these genes were not essential for haemin utilization in V. cholerae.</description><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins - genetics</subject><subject>Base Sequence</subject><subject>Biological Transport, Active - genetics</subject><subject>Cloning, Molecular</subject><subject>DNA Primers - genetics</subject><subject>DNA, Bacterial - genetics</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Escherichia coli Proteins</subject><subject>Genes, Bacterial</subject><subject>Genetic Complementation Test</subject><subject>Genetic Linkage</subject><subject>Heme - metabolism</subject><subject>Iron - metabolism</subject><subject>Membrane Proteins - genetics</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Restriction Mapping</subject><subject>Species Specificity</subject><subject>Vibrio cholerae</subject><subject>Vibrio cholerae - genetics</subject><subject>Vibrio cholerae - metabolism</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1P2zAYx61pCArbR5hkcdiJBL_HRuIwylsl0C7btJvlJE_WlDTu7FRtv_0SUoG0C1zsx_q_SH5-CGFKUkqEOl-klCuZMCN1So3RKaGEi3T7AU1ehI9oQowkCdfs9xE6jnFBCOVE8UN0aDJNtZYT1Pyq81B7XMx9A8EBroNvcRdcG1c-dBd47mD5-sZ_oIWIXQDc1O0TlLjz2LeAfYW7jccRuvg8-_bqDMM2H8_rMfcJHVSuifB5f5-gn7c3P6b3ycP3u9n020NSCK1FIkshtCxpnmesYoywnAkhHNVSlYxkRV4wA6wCZaTLnDayKkzpsopJp5gxGT9BX8feVfB_1xA7u6xjAU3jWvDraFVvMqrfxFtGmtF-TZr3xtP_jAu_Dm3_CUuNkpRrTnqTHk1F8DEGqOwq1EsXdpYSO2CzCzvQsQMdO2Czz9jsto9-2fev8yWUL8E9p16_HPVN3cDu3b328XE2TPwf82ylDg</recordid><startdate>199809</startdate><enddate>199809</enddate><creator>Occhino, Deborah A.</creator><creator>Wyckoff, Elizabeth E.</creator><creator>Henderson, Douglas P.</creator><creator>Wrona, Thomas J.</creator><creator>Payne, Shelley M.</creator><general>Blackwell Science Ltd, UK</general><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199809</creationdate><title>Vibrio cholerae iron transport: haem transport genes are linked to one of two sets of tonB, exbB, exbD genes</title><author>Occhino, Deborah A. ; Wyckoff, Elizabeth E. ; Henderson, Douglas P. ; Wrona, Thomas J. ; Payne, Shelley M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4884-5d4485d1bb72f2202b2444a1856d207cbc29e2fe695a7a895fc9da7f25a629973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Amino Acid Sequence</topic><topic>Bacterial Proteins - genetics</topic><topic>Base Sequence</topic><topic>Biological Transport, Active - genetics</topic><topic>Cloning, Molecular</topic><topic>DNA Primers - genetics</topic><topic>DNA, Bacterial - genetics</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Escherichia coli Proteins</topic><topic>Genes, Bacterial</topic><topic>Genetic Complementation Test</topic><topic>Genetic Linkage</topic><topic>Heme - metabolism</topic><topic>Iron - metabolism</topic><topic>Membrane Proteins - genetics</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Restriction Mapping</topic><topic>Species Specificity</topic><topic>Vibrio cholerae</topic><topic>Vibrio cholerae - genetics</topic><topic>Vibrio cholerae - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Occhino, Deborah A.</creatorcontrib><creatorcontrib>Wyckoff, Elizabeth E.</creatorcontrib><creatorcontrib>Henderson, Douglas P.</creatorcontrib><creatorcontrib>Wrona, Thomas J.</creatorcontrib><creatorcontrib>Payne, Shelley M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Occhino, Deborah A.</au><au>Wyckoff, Elizabeth E.</au><au>Henderson, Douglas P.</au><au>Wrona, Thomas J.</au><au>Payne, Shelley M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vibrio cholerae iron transport: haem transport genes are linked to one of two sets of tonB, exbB, exbD genes</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>1998-09</date><risdate>1998</risdate><volume>29</volume><issue>6</issue><spage>1493</spage><epage>1507</epage><pages>1493-1507</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Vibrio cholerae was found to have two sets of genes encoding TonB, ExbB and ExbD proteins. The first set (tonB1, exbB1, exbD1) was obtained by complementation of a V. cholerae tonB mutant. In the mutant, a plasmid containing these genes permitted transport via the known V. cholerae high‐affinity iron transport systems, including uptake of haem, vibriobactin and ferrichrome. When chromosomal mutations in exbB1 or exbD1 were introduced into a wild‐type V. cholerae background, no defect in iron transport was noted, indicating the existence of additional genes that can complement the defect in the wild‐type background. Another region of the V. cholerae chromosome was cloned that encoded a second functional TonB/Exb system (tonB2, exbB2, exbD2). A chromosomal mutation in exbB2 also failed to exhibit a defect in iron transport, but a V. cholerae strain that had chromosomal mutations in both the exbB1 and exbB2 genes displayed a mutant phenotype similar to that of an Escherichia coli tonB mutant. The genes encoding TonB1, ExbB1, ExbD1 were part of an operon that included three haem transport genes (hutBCD), and all six genes appeared to be expressed from a single Fur‐regulated promoter upstream of tonB1. A plasmid containing all six genes permitted utilization of haem by an E. coli strain expressing the V. cholerae haem receptor, HutA. Analysis of the hut genes indicated that hutBCD, which are predicted to encode a periplasmic binding protein (HutB) and cytoplasmic membrane permease (HutC and HutD), were required to reconstitute the V. cholerae haem transport system in E. coli. In V. cholerae, the presence of hutBCD stimulated growth when haemin was the iron source, but these genes were not essential for haemin utilization in V. cholerae.</abstract><cop>Oxford BSL</cop><pub>Blackwell Science Ltd, UK</pub><pmid>9781885</pmid><doi>10.1046/j.1365-2958.1998.01034.x</doi><tpages>15</tpages></addata></record> |
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subjects | Amino Acid Sequence Bacterial Proteins - genetics Base Sequence Biological Transport, Active - genetics Cloning, Molecular DNA Primers - genetics DNA, Bacterial - genetics Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli Proteins Genes, Bacterial Genetic Complementation Test Genetic Linkage Heme - metabolism Iron - metabolism Membrane Proteins - genetics Molecular Sequence Data Mutation Restriction Mapping Species Specificity Vibrio cholerae Vibrio cholerae - genetics Vibrio cholerae - metabolism |
title | Vibrio cholerae iron transport: haem transport genes are linked to one of two sets of tonB, exbB, exbD genes |
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