A DNA Probe for the Detection and Identification of Bacillus sporothermodurans Using the 16S-23S rDNA Spacer Region and Phylogenetic Analysis of some Field Isolates of Bacillus which form Highly Heat Resistant Spores

The spacer regions between the 16S and 23S rRNA genes (spacer regions 1) of Bacillus sporothermodurans were PCR-amplified, cloned and sequenced. Six unique spacer sequences in four size classes were recovered from two strains, rrnA (about 190 bp), rrnB (about 303 bp), rrnC (355 bp) and rrnD (554 bp)...

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Veröffentlicht in:	Systematic and applied microbiology 1998-08, Vol.21 (3), p.398-407
Hauptverfasser:	de Silva, Shyama, Petterson, Bertil, de Muro, Marilena Aquino, Priest, Fergus G.
Format:	Artikel
Sprache:	eng
Schlagworte:	16S rRNA Animals Bacillus - classification Bacillus - genetics Bacillus - growth & development Bacillus - isolation & purification Bacillus sporothermodurans Bacteriological methods and techniques used in bacteriology Bacteriology Base Sequence Biological and medical sciences Cattle DNA Probes DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Food industries Fundamental and applied biological sciences. Psychology Genes, rRNA Hot Temperature Microbiology milk Milk - microbiology Milk and cheese industries. Ice creams Molecular Sequence Data Operon Phylogeny RNA, Ribosomal, 16S - genetics RNA, Ribosomal, 23S - genetics rRNA spacer regions Sequence Analysis, DNA Silage - microbiology Spores, Bacterial tRNA
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