Development of in vitro screening system for assessment of antifilarial activity of compounds
Evaluation of antifilarial activity of new potential agents in vivo is extremely time consuming and uneconomic. In the present study effort has been made to develop an in vitro screening method using Acanthocheilonema viteae, a subcutaneously dwelling rodent filariid with anaerobic metabolic charact...
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| Veröffentlicht in: | Acta tropica 1998-07, Vol.70 (3), p.251-255 |
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| creator | Mukherjee, Monisha Misra, Shailja Chatterjee, R.K |
| description | Evaluation of antifilarial activity of new potential agents in vivo is extremely time consuming and uneconomic. In the present study effort has been made to develop an in vitro screening method using
Acanthocheilonema viteae, a subcutaneously dwelling rodent filariid with anaerobic metabolic characteristics like human filariids,
W. Bancrofti/
Brugia malayi as test parasite. Motility test and tetrazolium (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, MTT) based colorimetric assay were used as parameters in in vitro assay. Results showed that 92.3% of compounds (in vivo active) could be picked up in the in vitro assay when both adults and microfilarae (mf) were used simultaneously. Mf and adult stages separately detected, respectively, 84.6 and 69.2% of in vivo active compounds. The adults and mf separately and both the life stages together exhibited, respectively, 80.0, 50.0 and 80.0% false positive results in the in vitro test with in vivo inactive compounds. It is felt that mf stage when used in in vitro test using motility and MTT assays as parameters would be useful in primary screening of new potential filaricides. |
| doi_str_mv | 10.1016/S0001-706X(98)00016-3 |
| format | Article |
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Acanthocheilonema viteae, a subcutaneously dwelling rodent filariid with anaerobic metabolic characteristics like human filariids,
W. Bancrofti/
Brugia malayi as test parasite. Motility test and tetrazolium (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, MTT) based colorimetric assay were used as parameters in in vitro assay. Results showed that 92.3% of compounds (in vivo active) could be picked up in the in vitro assay when both adults and microfilarae (mf) were used simultaneously. Mf and adult stages separately detected, respectively, 84.6 and 69.2% of in vivo active compounds. The adults and mf separately and both the life stages together exhibited, respectively, 80.0, 50.0 and 80.0% false positive results in the in vitro test with in vivo inactive compounds. It is felt that mf stage when used in in vitro test using motility and MTT assays as parameters would be useful in primary screening of new potential filaricides.</description><identifier>ISSN: 0001-706X</identifier><identifier>EISSN: 1873-6254</identifier><identifier>DOI: 10.1016/S0001-706X(98)00016-3</identifier><identifier>PMID: 9777711</identifier><identifier>CODEN: ACTRAQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Acanthocheilonema viteae ; Animals ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antifilarials ; Antiparasitic agents ; Biological and medical sciences ; Colorimetry ; Coloring Agents - chemistry ; Dipetalonema - drug effects ; Dipetalonema - growth & development ; Dipetalonema - physiology ; Dipetalonema Infections - drug therapy ; Dipetalonema Infections - parasitology ; False Negative Reactions ; False Positive Reactions ; Female ; Filaricides - pharmacology ; In vitro screening ; Inhibition ; Male ; Medical sciences ; Microfilariae - drug effects ; Microfilariae - physiology ; Motility ; Movement - drug effects ; MTT ; Muridae ; Oxidation-Reduction ; Pharmacology. Drug treatments ; Predictive Value of Tests ; Tetrazolium Salts - chemistry ; Thiazoles - chemistry ; Tropical medicine</subject><ispartof>Acta tropica, 1998-07, Vol.70 (3), p.251-255</ispartof><rights>1998 Elsevier Science B.V.</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-8f4875e297408f6e765374492d1b7ca8520cfe46c5ac701bb8884614348cc1803</citedby><cites>FETCH-LOGICAL-c420t-8f4875e297408f6e765374492d1b7ca8520cfe46c5ac701bb8884614348cc1803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0001-706X(98)00016-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2417591$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9777711$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mukherjee, Monisha</creatorcontrib><creatorcontrib>Misra, Shailja</creatorcontrib><creatorcontrib>Chatterjee, R.K</creatorcontrib><title>Development of in vitro screening system for assessment of antifilarial activity of compounds</title><title>Acta tropica</title><addtitle>Acta Trop</addtitle><description>Evaluation of antifilarial activity of new potential agents in vivo is extremely time consuming and uneconomic. In the present study effort has been made to develop an in vitro screening method using
Acanthocheilonema viteae, a subcutaneously dwelling rodent filariid with anaerobic metabolic characteristics like human filariids,
W. Bancrofti/
Brugia malayi as test parasite. Motility test and tetrazolium (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, MTT) based colorimetric assay were used as parameters in in vitro assay. Results showed that 92.3% of compounds (in vivo active) could be picked up in the in vitro assay when both adults and microfilarae (mf) were used simultaneously. Mf and adult stages separately detected, respectively, 84.6 and 69.2% of in vivo active compounds. The adults and mf separately and both the life stages together exhibited, respectively, 80.0, 50.0 and 80.0% false positive results in the in vitro test with in vivo inactive compounds. It is felt that mf stage when used in in vitro test using motility and MTT assays as parameters would be useful in primary screening of new potential filaricides.</description><subject>Acanthocheilonema viteae</subject><subject>Animals</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antifilarials</subject><subject>Antiparasitic agents</subject><subject>Biological and medical sciences</subject><subject>Colorimetry</subject><subject>Coloring Agents - chemistry</subject><subject>Dipetalonema - drug effects</subject><subject>Dipetalonema - growth & development</subject><subject>Dipetalonema - physiology</subject><subject>Dipetalonema Infections - drug therapy</subject><subject>Dipetalonema Infections - parasitology</subject><subject>False Negative Reactions</subject><subject>False Positive Reactions</subject><subject>Female</subject><subject>Filaricides - pharmacology</subject><subject>In vitro screening</subject><subject>Inhibition</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microfilariae - drug effects</subject><subject>Microfilariae - physiology</subject><subject>Motility</subject><subject>Movement - drug effects</subject><subject>MTT</subject><subject>Muridae</subject><subject>Oxidation-Reduction</subject><subject>Pharmacology. Drug treatments</subject><subject>Predictive Value of Tests</subject><subject>Tetrazolium Salts - chemistry</subject><subject>Thiazoles - chemistry</subject><subject>Tropical medicine</subject><issn>0001-706X</issn><issn>1873-6254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1q3DAUhUVoSSZpHiHgRSjNwo3-LMmrUpL0BwJdJIVuitDIV0HBlqa6noF5-9iZ6Wyjjbic71yJj5ALRj8zytT1A6WU1ZqqP59aczUPqhZHZMGMFrXijXxHFgfkhJwiPk8T1w0_Jsetng5jC_L3FjbQ59UAaaxyqGKqNnEsuUJfAFJMTxVucYShCrlUDhEQ_7MujTHE3pXo-sr5MU7N7Rz4PKzyOnX4gbwPrkc4399n5Pe3u8ebH_X9r-8_b77e115yOtYmSKMb4K2W1AQFWjVCS9nyji21d6bh1AeQyjfOa8qWS2OMVEwKabxnhooz8nG3d1XyvzXgaIeIHvreJchrtKqdVhsl3wQFF0JJySaw2YG-ZMQCwa5KHFzZWkbt7N---rezXNsa--rfiql3sX9gvRygO7T2wqf8cp879K4PxSUf8YBxyXTTztiXHQaTtU2EYtFHSB66WMCPtsvxjY-8AOhuof4</recordid><startdate>19980730</startdate><enddate>19980730</enddate><creator>Mukherjee, Monisha</creator><creator>Misra, Shailja</creator><creator>Chatterjee, R.K</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>19980730</creationdate><title>Development of in vitro screening system for assessment of antifilarial activity of compounds</title><author>Mukherjee, Monisha ; Misra, Shailja ; Chatterjee, R.K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-8f4875e297408f6e765374492d1b7ca8520cfe46c5ac701bb8884614348cc1803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Acanthocheilonema viteae</topic><topic>Animals</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Antifilarials</topic><topic>Antiparasitic agents</topic><topic>Biological and medical sciences</topic><topic>Colorimetry</topic><topic>Coloring Agents - chemistry</topic><topic>Dipetalonema - drug effects</topic><topic>Dipetalonema - growth & development</topic><topic>Dipetalonema - physiology</topic><topic>Dipetalonema Infections - drug therapy</topic><topic>Dipetalonema Infections - parasitology</topic><topic>False Negative Reactions</topic><topic>False Positive Reactions</topic><topic>Female</topic><topic>Filaricides - pharmacology</topic><topic>In vitro screening</topic><topic>Inhibition</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microfilariae - drug effects</topic><topic>Microfilariae - physiology</topic><topic>Motility</topic><topic>Movement - drug effects</topic><topic>MTT</topic><topic>Muridae</topic><topic>Oxidation-Reduction</topic><topic>Pharmacology. Drug treatments</topic><topic>Predictive Value of Tests</topic><topic>Tetrazolium Salts - chemistry</topic><topic>Thiazoles - chemistry</topic><topic>Tropical medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mukherjee, Monisha</creatorcontrib><creatorcontrib>Misra, Shailja</creatorcontrib><creatorcontrib>Chatterjee, R.K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Acta tropica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mukherjee, Monisha</au><au>Misra, Shailja</au><au>Chatterjee, R.K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of in vitro screening system for assessment of antifilarial activity of compounds</atitle><jtitle>Acta tropica</jtitle><addtitle>Acta Trop</addtitle><date>1998-07-30</date><risdate>1998</risdate><volume>70</volume><issue>3</issue><spage>251</spage><epage>255</epage><pages>251-255</pages><issn>0001-706X</issn><eissn>1873-6254</eissn><coden>ACTRAQ</coden><abstract>Evaluation of antifilarial activity of new potential agents in vivo is extremely time consuming and uneconomic. In the present study effort has been made to develop an in vitro screening method using
Acanthocheilonema viteae, a subcutaneously dwelling rodent filariid with anaerobic metabolic characteristics like human filariids,
W. Bancrofti/
Brugia malayi as test parasite. Motility test and tetrazolium (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, MTT) based colorimetric assay were used as parameters in in vitro assay. Results showed that 92.3% of compounds (in vivo active) could be picked up in the in vitro assay when both adults and microfilarae (mf) were used simultaneously. Mf and adult stages separately detected, respectively, 84.6 and 69.2% of in vivo active compounds. The adults and mf separately and both the life stages together exhibited, respectively, 80.0, 50.0 and 80.0% false positive results in the in vitro test with in vivo inactive compounds. It is felt that mf stage when used in in vitro test using motility and MTT assays as parameters would be useful in primary screening of new potential filaricides.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>9777711</pmid><doi>10.1016/S0001-706X(98)00016-3</doi><tpages>5</tpages></addata></record> |
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| subjects | Acanthocheilonema viteae Animals Antibiotics. Antiinfectious agents. Antiparasitic agents Antifilarials Antiparasitic agents Biological and medical sciences Colorimetry Coloring Agents - chemistry Dipetalonema - drug effects Dipetalonema - growth & development Dipetalonema - physiology Dipetalonema Infections - drug therapy Dipetalonema Infections - parasitology False Negative Reactions False Positive Reactions Female Filaricides - pharmacology In vitro screening Inhibition Male Medical sciences Microfilariae - drug effects Microfilariae - physiology Motility Movement - drug effects MTT Muridae Oxidation-Reduction Pharmacology. Drug treatments Predictive Value of Tests Tetrazolium Salts - chemistry Thiazoles - chemistry Tropical medicine |
| title | Development of in vitro screening system for assessment of antifilarial activity of compounds |
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