Evidence for the presence of several phosphodiesterase isoforms in brown adipose tissue of Zucker rats: modulation of PDE2 by the fa gene expression
The present study was undertaken to characterise the phosphodiesterases (PDEs) present in brown adipose tissue (BAT) of Zucker rat pups and to determine whether the capacity for degradation of cyclic nucleotides was affected by the fatty genotype. Regardless of the genotype, PDE2–4 contributed to to...
Gespeichert in:
| Veröffentlicht in: | FEBS letters 1999-07, Vol.456 (1), p.207-210 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 210 |
|---|---|
| container_issue | 1 |
| container_start_page | 207 |
| container_title | FEBS letters |
| container_volume | 456 |
| creator | Coudray, Carole Charon, Christine Komas, Narcisse Mory, Gérard Diot-Dupuy, Francine Manganiello, Vincent Ferre, Pascal Bazin, Raymond |
| description | The present study was undertaken to characterise the phosphodiesterases (PDEs) present in brown adipose tissue (BAT) of Zucker rat pups and to determine whether the capacity for degradation of cyclic nucleotides was affected by the fatty genotype. Regardless of the genotype, PDE2–4 contributed to total PDE activity, the PDE3 activity equalling the sum of PDE2 and 4 activities. In fa/fa compared to Fa/fa rats, (a) PDE2 activity was significantly increased, (b) Western blot analysis of PDE2 revealed two signals at 71 and 105 kDa, with changes in protein being in good parallelism with changes in activity, (c) the PDE2 mRNA concentration was also significantly increased. In good agreement, the cGMP concentration was decreased in BAT from fa/fa pups. |
| doi_str_mv | 10.1016/S0014-5793(99)00934-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69970673</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0014579399009345</els_id><sourcerecordid>69970673</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4735-97734d50e58bd0d2b0bd2f0dc774349091df5b97523b7c5a22498478144a5d4e3</originalsourceid><addsrcrecordid>eNqNUcFu1DAQtRCILoVPAPmE4BCwY3sdc0FQthSpEkjAhYvlxBNqyMbBk2zZ_-CDcZIKcYODZc289-bZ8wh5yNkzzvj2-UfGuCyUNuKJMU8ZMyJXt8iGV1oUQm6r22Tzh3JC7iF-Y7muuLlLTjiTqlTKbMiv3SF46BugbUx0vAI6JMClEVuKcIDkOjpcRczHB8AxNxBowJgFe6Shp3WK1z11PgwxI2NAnBb1l6n5DokmN-ILuo9-6twYYj9DH97sSlofF8PW0a_QA4WfszVmxn1yp3UdwoOb-5R8Pt99OrsoLt-_fXf26rJopBaqMFoL6RUDVdWe-bJmtS9b5hutpZCGGe5bVRutSlHrRrmylKaSuuJSOuUliFPyeJ07pPhjyn-z-4ANdJ3rIU5ot8ZottUiE9VKbFJETNDaIYW9S0fLmZ3jsEscdt61NcYucViVdY9uDKZ6D_4v1br_TLhYCdehg-P_TbXnu9flgsyAMUt79nq5joK8sUOAZLEJc5A-JGhG62P4x2t_A7Tar6c</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69970673</pqid></control><display><type>article</type><title>Evidence for the presence of several phosphodiesterase isoforms in brown adipose tissue of Zucker rats: modulation of PDE2 by the fa gene expression</title><source>MEDLINE</source><source>Wiley Online Library Free Content</source><source>Access via Wiley Online Library</source><source>Access via ScienceDirect (Elsevier)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Coudray, Carole ; Charon, Christine ; Komas, Narcisse ; Mory, Gérard ; Diot-Dupuy, Francine ; Manganiello, Vincent ; Ferre, Pascal ; Bazin, Raymond</creator><creatorcontrib>Coudray, Carole ; Charon, Christine ; Komas, Narcisse ; Mory, Gérard ; Diot-Dupuy, Francine ; Manganiello, Vincent ; Ferre, Pascal ; Bazin, Raymond</creatorcontrib><description>The present study was undertaken to characterise the phosphodiesterases (PDEs) present in brown adipose tissue (BAT) of Zucker rat pups and to determine whether the capacity for degradation of cyclic nucleotides was affected by the fatty genotype. Regardless of the genotype, PDE2–4 contributed to total PDE activity, the PDE3 activity equalling the sum of PDE2 and 4 activities. In fa/fa compared to Fa/fa rats, (a) PDE2 activity was significantly increased, (b) Western blot analysis of PDE2 revealed two signals at 71 and 105 kDa, with changes in protein being in good parallelism with changes in activity, (c) the PDE2 mRNA concentration was also significantly increased. In good agreement, the cGMP concentration was decreased in BAT from fa/fa pups.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(99)00934-5</identifier><identifier>PMID: 10452559</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Adipose Tissue, Brown - enzymology ; Animals ; Animals, Newborn ; Blotting, Northern ; Cilostamide, N-cyclohexyl-N-methyl-4-(1,2-dihydro-2-oxo-6-quinolyloxy) butiramide ; Cyclic AMP - metabolism ; Cyclic GMP ; Cyclic GMP - metabolism ; Cytosol - enzymology ; Female ; Gene Expression Regulation ; Immunoblotting ; Isoenzymes - genetics ; Isoenzymes - metabolism ; Leptin ; Male ; Obesity ; Obesity - genetics ; Phosphoric Diester Hydrolases - genetics ; Phosphoric Diester Hydrolases - metabolism ; Proteins - genetics ; Rats ; Rats, Zucker ; TES, N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid ; Type 3 phosphodiesterase ; Type 4 phosphodiesterase</subject><ispartof>FEBS letters, 1999-07, Vol.456 (1), p.207-210</ispartof><rights>1999 Federation of European Biochemical Societies</rights><rights>FEBS Letters 456 (1999) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4735-97734d50e58bd0d2b0bd2f0dc774349091df5b97523b7c5a22498478144a5d4e3</citedby><cites>FETCH-LOGICAL-c4735-97734d50e58bd0d2b0bd2f0dc774349091df5b97523b7c5a22498478144a5d4e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2899%2900934-5$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0014-5793(99)00934-5$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1417,1433,3550,27924,27925,45574,45575,45995,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10452559$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Coudray, Carole</creatorcontrib><creatorcontrib>Charon, Christine</creatorcontrib><creatorcontrib>Komas, Narcisse</creatorcontrib><creatorcontrib>Mory, Gérard</creatorcontrib><creatorcontrib>Diot-Dupuy, Francine</creatorcontrib><creatorcontrib>Manganiello, Vincent</creatorcontrib><creatorcontrib>Ferre, Pascal</creatorcontrib><creatorcontrib>Bazin, Raymond</creatorcontrib><title>Evidence for the presence of several phosphodiesterase isoforms in brown adipose tissue of Zucker rats: modulation of PDE2 by the fa gene expression</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The present study was undertaken to characterise the phosphodiesterases (PDEs) present in brown adipose tissue (BAT) of Zucker rat pups and to determine whether the capacity for degradation of cyclic nucleotides was affected by the fatty genotype. Regardless of the genotype, PDE2–4 contributed to total PDE activity, the PDE3 activity equalling the sum of PDE2 and 4 activities. In fa/fa compared to Fa/fa rats, (a) PDE2 activity was significantly increased, (b) Western blot analysis of PDE2 revealed two signals at 71 and 105 kDa, with changes in protein being in good parallelism with changes in activity, (c) the PDE2 mRNA concentration was also significantly increased. In good agreement, the cGMP concentration was decreased in BAT from fa/fa pups.</description><subject>Adipose Tissue, Brown - enzymology</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Blotting, Northern</subject><subject>Cilostamide, N-cyclohexyl-N-methyl-4-(1,2-dihydro-2-oxo-6-quinolyloxy) butiramide</subject><subject>Cyclic AMP - metabolism</subject><subject>Cyclic GMP</subject><subject>Cyclic GMP - metabolism</subject><subject>Cytosol - enzymology</subject><subject>Female</subject><subject>Gene Expression Regulation</subject><subject>Immunoblotting</subject><subject>Isoenzymes - genetics</subject><subject>Isoenzymes - metabolism</subject><subject>Leptin</subject><subject>Male</subject><subject>Obesity</subject><subject>Obesity - genetics</subject><subject>Phosphoric Diester Hydrolases - genetics</subject><subject>Phosphoric Diester Hydrolases - metabolism</subject><subject>Proteins - genetics</subject><subject>Rats</subject><subject>Rats, Zucker</subject><subject>TES, N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid</subject><subject>Type 3 phosphodiesterase</subject><subject>Type 4 phosphodiesterase</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUcFu1DAQtRCILoVPAPmE4BCwY3sdc0FQthSpEkjAhYvlxBNqyMbBk2zZ_-CDcZIKcYODZc289-bZ8wh5yNkzzvj2-UfGuCyUNuKJMU8ZMyJXt8iGV1oUQm6r22Tzh3JC7iF-Y7muuLlLTjiTqlTKbMiv3SF46BugbUx0vAI6JMClEVuKcIDkOjpcRczHB8AxNxBowJgFe6Shp3WK1z11PgwxI2NAnBb1l6n5DokmN-ILuo9-6twYYj9DH97sSlofF8PW0a_QA4WfszVmxn1yp3UdwoOb-5R8Pt99OrsoLt-_fXf26rJopBaqMFoL6RUDVdWe-bJmtS9b5hutpZCGGe5bVRutSlHrRrmylKaSuuJSOuUliFPyeJ07pPhjyn-z-4ANdJ3rIU5ot8ZottUiE9VKbFJETNDaIYW9S0fLmZ3jsEscdt61NcYucViVdY9uDKZ6D_4v1br_TLhYCdehg-P_TbXnu9flgsyAMUt79nq5joK8sUOAZLEJc5A-JGhG62P4x2t_A7Tar6c</recordid><startdate>19990730</startdate><enddate>19990730</enddate><creator>Coudray, Carole</creator><creator>Charon, Christine</creator><creator>Komas, Narcisse</creator><creator>Mory, Gérard</creator><creator>Diot-Dupuy, Francine</creator><creator>Manganiello, Vincent</creator><creator>Ferre, Pascal</creator><creator>Bazin, Raymond</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990730</creationdate><title>Evidence for the presence of several phosphodiesterase isoforms in brown adipose tissue of Zucker rats: modulation of PDE2 by the fa gene expression</title><author>Coudray, Carole ; Charon, Christine ; Komas, Narcisse ; Mory, Gérard ; Diot-Dupuy, Francine ; Manganiello, Vincent ; Ferre, Pascal ; Bazin, Raymond</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4735-97734d50e58bd0d2b0bd2f0dc774349091df5b97523b7c5a22498478144a5d4e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adipose Tissue, Brown - enzymology</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Blotting, Northern</topic><topic>Cilostamide, N-cyclohexyl-N-methyl-4-(1,2-dihydro-2-oxo-6-quinolyloxy) butiramide</topic><topic>Cyclic AMP - metabolism</topic><topic>Cyclic GMP</topic><topic>Cyclic GMP - metabolism</topic><topic>Cytosol - enzymology</topic><topic>Female</topic><topic>Gene Expression Regulation</topic><topic>Immunoblotting</topic><topic>Isoenzymes - genetics</topic><topic>Isoenzymes - metabolism</topic><topic>Leptin</topic><topic>Male</topic><topic>Obesity</topic><topic>Obesity - genetics</topic><topic>Phosphoric Diester Hydrolases - genetics</topic><topic>Phosphoric Diester Hydrolases - metabolism</topic><topic>Proteins - genetics</topic><topic>Rats</topic><topic>Rats, Zucker</topic><topic>TES, N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid</topic><topic>Type 3 phosphodiesterase</topic><topic>Type 4 phosphodiesterase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Coudray, Carole</creatorcontrib><creatorcontrib>Charon, Christine</creatorcontrib><creatorcontrib>Komas, Narcisse</creatorcontrib><creatorcontrib>Mory, Gérard</creatorcontrib><creatorcontrib>Diot-Dupuy, Francine</creatorcontrib><creatorcontrib>Manganiello, Vincent</creatorcontrib><creatorcontrib>Ferre, Pascal</creatorcontrib><creatorcontrib>Bazin, Raymond</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coudray, Carole</au><au>Charon, Christine</au><au>Komas, Narcisse</au><au>Mory, Gérard</au><au>Diot-Dupuy, Francine</au><au>Manganiello, Vincent</au><au>Ferre, Pascal</au><au>Bazin, Raymond</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for the presence of several phosphodiesterase isoforms in brown adipose tissue of Zucker rats: modulation of PDE2 by the fa gene expression</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1999-07-30</date><risdate>1999</risdate><volume>456</volume><issue>1</issue><spage>207</spage><epage>210</epage><pages>207-210</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>The present study was undertaken to characterise the phosphodiesterases (PDEs) present in brown adipose tissue (BAT) of Zucker rat pups and to determine whether the capacity for degradation of cyclic nucleotides was affected by the fatty genotype. Regardless of the genotype, PDE2–4 contributed to total PDE activity, the PDE3 activity equalling the sum of PDE2 and 4 activities. In fa/fa compared to Fa/fa rats, (a) PDE2 activity was significantly increased, (b) Western blot analysis of PDE2 revealed two signals at 71 and 105 kDa, with changes in protein being in good parallelism with changes in activity, (c) the PDE2 mRNA concentration was also significantly increased. In good agreement, the cGMP concentration was decreased in BAT from fa/fa pups.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>10452559</pmid><doi>10.1016/S0014-5793(99)00934-5</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1999-07, Vol.456 (1), p.207-210 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69970673 |
| source | MEDLINE; Wiley Online Library Free Content; Access via Wiley Online Library; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adipose Tissue, Brown - enzymology Animals Animals, Newborn Blotting, Northern Cilostamide, N-cyclohexyl-N-methyl-4-(1,2-dihydro-2-oxo-6-quinolyloxy) butiramide Cyclic AMP - metabolism Cyclic GMP Cyclic GMP - metabolism Cytosol - enzymology Female Gene Expression Regulation Immunoblotting Isoenzymes - genetics Isoenzymes - metabolism Leptin Male Obesity Obesity - genetics Phosphoric Diester Hydrolases - genetics Phosphoric Diester Hydrolases - metabolism Proteins - genetics Rats Rats, Zucker TES, N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid Type 3 phosphodiesterase Type 4 phosphodiesterase |
| title | Evidence for the presence of several phosphodiesterase isoforms in brown adipose tissue of Zucker rats: modulation of PDE2 by the fa gene expression |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T20%3A18%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evidence%20for%20the%20presence%20of%20several%20phosphodiesterase%20isoforms%20in%20brown%20adipose%20tissue%20of%20Zucker%20rats:%20modulation%20of%20PDE2%20by%20the%20fa%20gene%20expression&rft.jtitle=FEBS%20letters&rft.au=Coudray,%20Carole&rft.date=1999-07-30&rft.volume=456&rft.issue=1&rft.spage=207&rft.epage=210&rft.pages=207-210&rft.issn=0014-5793&rft.eissn=1873-3468&rft_id=info:doi/10.1016/S0014-5793(99)00934-5&rft_dat=%3Cproquest_cross%3E69970673%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69970673&rft_id=info:pmid/10452559&rft_els_id=S0014579399009345&rfr_iscdi=true |