Dogs are definitive hosts of Neospora caninum
Dogs were investigated to determine if they are definitive hosts of Neospora caninum. Four dogs were fed N. caninum tissue cysts in infected mouse tissue, and two negative control dogs were fed uninfected mouse tissue. Dog faeces were examined daily for 30 days using a sucrose flotation technique. T...
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| Veröffentlicht in: | International journal for parasitology 1998-09, Vol.28 (9), p.1473-1478 |
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| container_title | International journal for parasitology |
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| creator | MCALLISTER, M. M DUBEY, J. P LINDSAY, D. S JOLLEY, W. R WILLS, R. A MCGUIRE, A. M |
| description | Dogs were investigated to determine if they are definitive hosts of Neospora caninum. Four dogs were fed N. caninum tissue cysts in infected mouse tissue, and two negative control dogs were fed uninfected mouse tissue. Dog faeces were examined daily for 30 days using a sucrose flotation technique. Three challenged dogs shed spherical to subspherical unsporulated oocysts, measuring 10 to 11 microns in diameter. Oocysts sporulated within 3 days and contained two sporocysts, each with four sporozoites. Outbred, inbred, and gamma-interferon knockout mice were inoculated with canine faecal extracts and monitored for evidence of neosporosis using a variety of morphologic, immunohistologic, serologic, and genetic analyses. Mice that received faeces from each dog observed to shed oocysts were demonstrated to have neosporosis by two or more techniques. One mouse was demonstrated to be infected with N. caninum by immunohistochemistry, ultrastructural analysis, and a species-specific PCR test. No evidence of neosporosis was observed in control animals. Based on this study, dogs are a definitive host of Neospora caninum. |
| doi_str_mv | 10.1016/S0020-7519(98)00138-6 |
| format | Article |
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M ; DUBEY, J. P ; LINDSAY, D. S ; JOLLEY, W. R ; WILLS, R. A ; MCGUIRE, A. M</creator><creatorcontrib>MCALLISTER, M. M ; DUBEY, J. P ; LINDSAY, D. S ; JOLLEY, W. R ; WILLS, R. A ; MCGUIRE, A. M</creatorcontrib><description>Dogs were investigated to determine if they are definitive hosts of Neospora caninum. Four dogs were fed N. caninum tissue cysts in infected mouse tissue, and two negative control dogs were fed uninfected mouse tissue. Dog faeces were examined daily for 30 days using a sucrose flotation technique. Three challenged dogs shed spherical to subspherical unsporulated oocysts, measuring 10 to 11 microns in diameter. Oocysts sporulated within 3 days and contained two sporocysts, each with four sporozoites. Outbred, inbred, and gamma-interferon knockout mice were inoculated with canine faecal extracts and monitored for evidence of neosporosis using a variety of morphologic, immunohistologic, serologic, and genetic analyses. Mice that received faeces from each dog observed to shed oocysts were demonstrated to have neosporosis by two or more techniques. One mouse was demonstrated to be infected with N. caninum by immunohistochemistry, ultrastructural analysis, and a species-specific PCR test. No evidence of neosporosis was observed in control animals. Based on this study, dogs are a definitive host of Neospora caninum.</description><identifier>ISSN: 0020-7519</identifier><identifier>EISSN: 1879-0135</identifier><identifier>DOI: 10.1016/S0020-7519(98)00138-6</identifier><identifier>PMID: 9770635</identifier><identifier>CODEN: IJPYBT</identifier><language>eng</language><publisher>Oxford: Elsevier Science</publisher><subject>Animal protozoal diseases ; Animals ; Antibodies, Monoclonal ; Antibodies, Protozoan ; Antibody Specificity ; Biological and medical sciences ; Brain - parasitology ; Cells, Cultured - parasitology ; Coccidiosis - pathology ; Coccidiosis - veterinary ; Disease Vectors ; DNA, Protozoan - analysis ; Dog Diseases - parasitology ; Dog Diseases - pathology ; Dogs ; Feces - parasitology ; Fluorescent Antibody Technique, Indirect ; Fundamental and applied biological sciences. Psychology ; Host-Parasite Interactions ; Immunohistochemistry ; Infectious diseases ; Life Cycle Stages ; Life cycle. Host-agent relationship. Pathogenesis ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Mice, Knockout ; Neospora - immunology ; Neospora - isolation & purification ; Neospora caninum ; neosporosis ; Parasitic diseases ; Polymerase Chain Reaction ; Protozoa ; Protozoal diseases</subject><ispartof>International journal for parasitology, 1998-09, Vol.28 (9), p.1473-1478</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1596154$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9770635$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MCALLISTER, M. M</creatorcontrib><creatorcontrib>DUBEY, J. P</creatorcontrib><creatorcontrib>LINDSAY, D. S</creatorcontrib><creatorcontrib>JOLLEY, W. R</creatorcontrib><creatorcontrib>WILLS, R. A</creatorcontrib><creatorcontrib>MCGUIRE, A. M</creatorcontrib><title>Dogs are definitive hosts of Neospora caninum</title><title>International journal for parasitology</title><addtitle>Int J Parasitol</addtitle><description>Dogs were investigated to determine if they are definitive hosts of Neospora caninum. Four dogs were fed N. caninum tissue cysts in infected mouse tissue, and two negative control dogs were fed uninfected mouse tissue. Dog faeces were examined daily for 30 days using a sucrose flotation technique. Three challenged dogs shed spherical to subspherical unsporulated oocysts, measuring 10 to 11 microns in diameter. Oocysts sporulated within 3 days and contained two sporocysts, each with four sporozoites. Outbred, inbred, and gamma-interferon knockout mice were inoculated with canine faecal extracts and monitored for evidence of neosporosis using a variety of morphologic, immunohistologic, serologic, and genetic analyses. Mice that received faeces from each dog observed to shed oocysts were demonstrated to have neosporosis by two or more techniques. One mouse was demonstrated to be infected with N. caninum by immunohistochemistry, ultrastructural analysis, and a species-specific PCR test. No evidence of neosporosis was observed in control animals. Based on this study, dogs are a definitive host of Neospora caninum.</description><subject>Animal protozoal diseases</subject><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Antibodies, Protozoan</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Brain - parasitology</subject><subject>Cells, Cultured - parasitology</subject><subject>Coccidiosis - pathology</subject><subject>Coccidiosis - veterinary</subject><subject>Disease Vectors</subject><subject>DNA, Protozoan - analysis</subject><subject>Dog Diseases - parasitology</subject><subject>Dog Diseases - pathology</subject><subject>Dogs</subject><subject>Feces - parasitology</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Host-Parasite Interactions</subject><subject>Immunohistochemistry</subject><subject>Infectious diseases</subject><subject>Life Cycle Stages</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Knockout</subject><subject>Neospora - immunology</subject><subject>Neospora - isolation & purification</subject><subject>Neospora caninum</subject><subject>neosporosis</subject><subject>Parasitic diseases</subject><subject>Polymerase Chain Reaction</subject><subject>Protozoa</subject><subject>Protozoal diseases</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0EtLxDAUBeAgyjiO_oSBLkR0Ec1t81zK-IRBF-q63LaJRtqmNq3gv7dgma2rw-V8nMUlZA3sEhjIqxfGUkaVAHNu9AVjkGkq98gStDJ0usQ-We7IITmK8XNCIuN8QRZGKSYzsST0JrzHBHubVNb51g_-2yYfIQ4xCS55siF2ocekxNa3Y3NMDhzW0Z7MuSJvd7evmwe6fb5_3FxvaZdKOVAstAbUqKEqrDPIucrSEjlIkaVSQGEUOKdT1BIZTInIOLdOV6YSKZpsRc7-drs-fI02DnnjY2nrGlsbxphLY6TRQv0LQQllgLEJrmc4Fo2t8q73DfY_-fyIqT-de4wl1q7HtvRxx0AYCYJnvzkRa4U</recordid><startdate>19980901</startdate><enddate>19980901</enddate><creator>MCALLISTER, M. 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Psychology</topic><topic>Host-Parasite Interactions</topic><topic>Immunohistochemistry</topic><topic>Infectious diseases</topic><topic>Life Cycle Stages</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Knockout</topic><topic>Neospora - immunology</topic><topic>Neospora - isolation & purification</topic><topic>Neospora caninum</topic><topic>neosporosis</topic><topic>Parasitic diseases</topic><topic>Polymerase Chain Reaction</topic><topic>Protozoa</topic><topic>Protozoal diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MCALLISTER, M. M</creatorcontrib><creatorcontrib>DUBEY, J. P</creatorcontrib><creatorcontrib>LINDSAY, D. S</creatorcontrib><creatorcontrib>JOLLEY, W. R</creatorcontrib><creatorcontrib>WILLS, R. A</creatorcontrib><creatorcontrib>MCGUIRE, A. 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Outbred, inbred, and gamma-interferon knockout mice were inoculated with canine faecal extracts and monitored for evidence of neosporosis using a variety of morphologic, immunohistologic, serologic, and genetic analyses. Mice that received faeces from each dog observed to shed oocysts were demonstrated to have neosporosis by two or more techniques. One mouse was demonstrated to be infected with N. caninum by immunohistochemistry, ultrastructural analysis, and a species-specific PCR test. No evidence of neosporosis was observed in control animals. Based on this study, dogs are a definitive host of Neospora caninum.</abstract><cop>Oxford</cop><pub>Elsevier Science</pub><pmid>9770635</pmid><doi>10.1016/S0020-7519(98)00138-6</doi><tpages>6</tpages></addata></record> |
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| subjects | Animal protozoal diseases Animals Antibodies, Monoclonal Antibodies, Protozoan Antibody Specificity Biological and medical sciences Brain - parasitology Cells, Cultured - parasitology Coccidiosis - pathology Coccidiosis - veterinary Disease Vectors DNA, Protozoan - analysis Dog Diseases - parasitology Dog Diseases - pathology Dogs Feces - parasitology Fluorescent Antibody Technique, Indirect Fundamental and applied biological sciences. Psychology Host-Parasite Interactions Immunohistochemistry Infectious diseases Life Cycle Stages Life cycle. Host-agent relationship. Pathogenesis Medical sciences Mice Mice, Inbred BALB C Mice, Knockout Neospora - immunology Neospora - isolation & purification Neospora caninum neosporosis Parasitic diseases Polymerase Chain Reaction Protozoa Protozoal diseases |
| title | Dogs are definitive hosts of Neospora caninum |
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