Role of Fe(III) in Fe(II)citrate-mediated peroxidation of mitochondrial membrane lipids
In this report we study the effect of Fe(III) on lipid peroxidation induced by Fe(II)citrate in mitochondrial membranes, as assessed by the production of thiobarbituric acid-reactive substances and antimycin A-insensitive oxygen uptake. The presence of Fe(III) stimulates initiation of lipid peroxida...
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| Veröffentlicht in: | Molecular and cellular biochemistry 1999-06, Vol.196 (1/2), p.163-168 |
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| creator | Castilho, R.F Meinicke, A.R Vercesi, A.E Hermes-Lima, M |
| description | In this report we study the effect of Fe(III) on lipid peroxidation induced by Fe(II)citrate in mitochondrial membranes, as assessed by the production of thiobarbituric acid-reactive substances and antimycin A-insensitive oxygen uptake. The presence of Fe(III) stimulates initiation of lipid peroxidation when low citrate:Fe(II) ratios are used (< or = 4:1). For a citrate:total iron ratio of 1:1 the maximal stimulation of lipid peroxidation by Fe(III) was observed when the Fe(II):Fe(III) ratio was in the range of 1:1 to 1:2. The lag phase that accompanies oxygen uptake was greatly diminished by increasing concentrations of Fe(III) when the citrate:total iron ratio was 1:1, but not when this ratio was higher. It is concluded that the increase of lipid peroxidation by Fe(III) is observed only when low citrate:Fe(II) ratios were used. Similar results were obtained using ATP as a ligand of iron. Monitoring the rate of spontaneous Fe(II) oxidation by measuring oxygen uptake in buffered medium, in the absence of mitochondria, Fe(III)-stimulated oxygen consumption was observed only when a low citrate:Fe(II) ratio was used. This result suggests that Fe(III) may facilitate the initiation and/or propagation of lipid peroxidation by increasing the rate of Fe(II)citrate-generated reactive oxygen species. |
| doi_str_mv | 10.1023/A:1006988129221 |
| format | Article |
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The presence of Fe(III) stimulates initiation of lipid peroxidation when low citrate:Fe(II) ratios are used (< or = 4:1). For a citrate:total iron ratio of 1:1 the maximal stimulation of lipid peroxidation by Fe(III) was observed when the Fe(II):Fe(III) ratio was in the range of 1:1 to 1:2. The lag phase that accompanies oxygen uptake was greatly diminished by increasing concentrations of Fe(III) when the citrate:total iron ratio was 1:1, but not when this ratio was higher. It is concluded that the increase of lipid peroxidation by Fe(III) is observed only when low citrate:Fe(II) ratios were used. Similar results were obtained using ATP as a ligand of iron. Monitoring the rate of spontaneous Fe(II) oxidation by measuring oxygen uptake in buffered medium, in the absence of mitochondria, Fe(III)-stimulated oxygen consumption was observed only when a low citrate:Fe(II) ratio was used. This result suggests that Fe(III) may facilitate the initiation and/or propagation of lipid peroxidation by increasing the rate of Fe(II)citrate-generated reactive oxygen species.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1023/A:1006988129221</identifier><identifier>PMID: 10448916</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Animals ; citrates ; Ferric Compounds - metabolism ; ferric ions ; Ferrous Compounds - metabolism ; ferrous ions ; free radicals ; Intracellular Membranes - metabolism ; Iron ; Lipid Peroxidation ; Lipids ; Membrane Lipids - metabolism ; Mitochondria - metabolism ; Oxygen ; Oxygen Consumption ; Oxygen uptake ; Peroxidation ; Rats ; Rats, Wistar ; reactive oxygen species</subject><ispartof>Molecular and cellular biochemistry, 1999-06, Vol.196 (1/2), p.163-168</ispartof><rights>Kluwer Academic Publishers 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c304t-55f411a62a9ea2b743d4ec92dbfb03752553cd99d86a41341509a0bed1dc9e373</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10448916$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Castilho, R.F</creatorcontrib><creatorcontrib>Meinicke, A.R</creatorcontrib><creatorcontrib>Vercesi, A.E</creatorcontrib><creatorcontrib>Hermes-Lima, M</creatorcontrib><title>Role of Fe(III) in Fe(II)citrate-mediated peroxidation of mitochondrial membrane lipids</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><description>In this report we study the effect of Fe(III) on lipid peroxidation induced by Fe(II)citrate in mitochondrial membranes, as assessed by the production of thiobarbituric acid-reactive substances and antimycin A-insensitive oxygen uptake. The presence of Fe(III) stimulates initiation of lipid peroxidation when low citrate:Fe(II) ratios are used (< or = 4:1). For a citrate:total iron ratio of 1:1 the maximal stimulation of lipid peroxidation by Fe(III) was observed when the Fe(II):Fe(III) ratio was in the range of 1:1 to 1:2. The lag phase that accompanies oxygen uptake was greatly diminished by increasing concentrations of Fe(III) when the citrate:total iron ratio was 1:1, but not when this ratio was higher. It is concluded that the increase of lipid peroxidation by Fe(III) is observed only when low citrate:Fe(II) ratios were used. Similar results were obtained using ATP as a ligand of iron. Monitoring the rate of spontaneous Fe(II) oxidation by measuring oxygen uptake in buffered medium, in the absence of mitochondria, Fe(III)-stimulated oxygen consumption was observed only when a low citrate:Fe(II) ratio was used. This result suggests that Fe(III) may facilitate the initiation and/or propagation of lipid peroxidation by increasing the rate of Fe(II)citrate-generated reactive oxygen species.</description><subject>Animals</subject><subject>citrates</subject><subject>Ferric Compounds - metabolism</subject><subject>ferric ions</subject><subject>Ferrous Compounds - metabolism</subject><subject>ferrous ions</subject><subject>free radicals</subject><subject>Intracellular Membranes - metabolism</subject><subject>Iron</subject><subject>Lipid Peroxidation</subject><subject>Lipids</subject><subject>Membrane Lipids - metabolism</subject><subject>Mitochondria - metabolism</subject><subject>Oxygen</subject><subject>Oxygen Consumption</subject><subject>Oxygen uptake</subject><subject>Peroxidation</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>reactive oxygen species</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkTFPwzAQhS0EoqUws0HEgOgQ8NlOHLNVFYVIlZCAijFyYgdcJXGxEwn-PYaWhend8L3Tu3cInQK-BkzozewWME5FlgERhMAeGkPCacwEiH00xhTjOAPOR-jI-zXGAQY4RCPAjGUC0jF6fbKNjmwdLfRVnufTyHTbcVqZ3slex61WJqiKNtrZT6Nkb2z342hNb6t32ylnZBO1ui2d7HTUmI1R_hgd1LLx-mSnE7Ra3L3MH-Ll430-ny3jimLWx0lSMwCZEim0JCVnVDFdCaLKusSUJyRJaKWEUFkqGVAGCRYSl1qBqoSmnE7Q5XbvxtmPQfu-aI2vdNOEKHbwRSpEmhFOAnjxD1zbwXUhW8GTlITGfqGzHTSU4e5i40wr3Vfx11cAzrdALW0h35zxxeqZYKCYCMrDE-g39I9z0A</recordid><startdate>19990601</startdate><enddate>19990601</enddate><creator>Castilho, R.F</creator><creator>Meinicke, A.R</creator><creator>Vercesi, A.E</creator><creator>Hermes-Lima, M</creator><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19990601</creationdate><title>Role of Fe(III) in Fe(II)citrate-mediated peroxidation of mitochondrial membrane lipids</title><author>Castilho, R.F ; Meinicke, A.R ; Vercesi, A.E ; Hermes-Lima, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c304t-55f411a62a9ea2b743d4ec92dbfb03752553cd99d86a41341509a0bed1dc9e373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>citrates</topic><topic>Ferric Compounds - metabolism</topic><topic>ferric ions</topic><topic>Ferrous Compounds - metabolism</topic><topic>ferrous ions</topic><topic>free radicals</topic><topic>Intracellular Membranes - metabolism</topic><topic>Iron</topic><topic>Lipid Peroxidation</topic><topic>Lipids</topic><topic>Membrane Lipids - metabolism</topic><topic>Mitochondria - metabolism</topic><topic>Oxygen</topic><topic>Oxygen Consumption</topic><topic>Oxygen uptake</topic><topic>Peroxidation</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>reactive oxygen species</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Castilho, R.F</creatorcontrib><creatorcontrib>Meinicke, A.R</creatorcontrib><creatorcontrib>Vercesi, A.E</creatorcontrib><creatorcontrib>Hermes-Lima, M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Castilho, R.F</au><au>Meinicke, A.R</au><au>Vercesi, A.E</au><au>Hermes-Lima, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of Fe(III) in Fe(II)citrate-mediated peroxidation of mitochondrial membrane lipids</atitle><jtitle>Molecular and cellular biochemistry</jtitle><addtitle>Mol Cell Biochem</addtitle><date>1999-06-01</date><risdate>1999</risdate><volume>196</volume><issue>1/2</issue><spage>163</spage><epage>168</epage><pages>163-168</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>In this report we study the effect of Fe(III) on lipid peroxidation induced by Fe(II)citrate in mitochondrial membranes, as assessed by the production of thiobarbituric acid-reactive substances and antimycin A-insensitive oxygen uptake. The presence of Fe(III) stimulates initiation of lipid peroxidation when low citrate:Fe(II) ratios are used (< or = 4:1). For a citrate:total iron ratio of 1:1 the maximal stimulation of lipid peroxidation by Fe(III) was observed when the Fe(II):Fe(III) ratio was in the range of 1:1 to 1:2. The lag phase that accompanies oxygen uptake was greatly diminished by increasing concentrations of Fe(III) when the citrate:total iron ratio was 1:1, but not when this ratio was higher. It is concluded that the increase of lipid peroxidation by Fe(III) is observed only when low citrate:Fe(II) ratios were used. Similar results were obtained using ATP as a ligand of iron. Monitoring the rate of spontaneous Fe(II) oxidation by measuring oxygen uptake in buffered medium, in the absence of mitochondria, Fe(III)-stimulated oxygen consumption was observed only when a low citrate:Fe(II) ratio was used. This result suggests that Fe(III) may facilitate the initiation and/or propagation of lipid peroxidation by increasing the rate of Fe(II)citrate-generated reactive oxygen species.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>10448916</pmid><doi>10.1023/A:1006988129221</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals citrates Ferric Compounds - metabolism ferric ions Ferrous Compounds - metabolism ferrous ions free radicals Intracellular Membranes - metabolism Iron Lipid Peroxidation Lipids Membrane Lipids - metabolism Mitochondria - metabolism Oxygen Oxygen Consumption Oxygen uptake Peroxidation Rats Rats, Wistar reactive oxygen species |
| title | Role of Fe(III) in Fe(II)citrate-mediated peroxidation of mitochondrial membrane lipids |
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