Acute Inhibition of Spontaneous Uterine Contractions by an Estrogenic Polychlorinated Biphenyl Is Associated with Disruption of Gap Junctional Communication
An estrogenic polychlorinated biphenyl, 4-hydroxy-2′,4′,6′-trichlorobiphenyl (4-OH-TCB), inhibits oscillatory uterine contractions immediately. Because increased gap junction formation is associated with the development of synchronized uterine contractions at term, we examined whether the inhibitory...
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| Veröffentlicht in: | Toxicology and applied pharmacology 1998-09, Vol.152 (1), p.18-29 |
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| creator | Tsai, Mei-Ling Cesen-Cummings, Kimberley Webb, R.Clinton Loch-Caruso, Rita |
| description | An estrogenic polychlorinated biphenyl, 4-hydroxy-2′,4′,6′-trichlorobiphenyl (4-OH-TCB), inhibits oscillatory uterine contractions immediately. Because increased gap junction formation is associated with the development of synchronized uterine contractions at term, we examined whether the inhibitory effect of 4-OH-TCB on spontaneous oscillatory contractions was due to the disruption of gap junctional communication. The effect of 4-OH-TCB on gap junctional communication was determined by intercellular Lucifer yellow dye transfer in primary cultures of myometrial myocytes isolated from midgestation rats. Intercellular dye transfer was inhibited by 4-OH-TCB or 17β-estradiol in a concentration-dependent manner. The inhibitory effect of 4-OH-TCB on intercellular dye transfer was reversed by tetraethylammonium (TEA). To examine effects on uterine contraction, longitudinal uterine strips were excised from midgestation rats and placed in muscle baths for isometric force measurement. Spontaneous uterine oscillation was suppressed by 4-OH-TCB or 17β-estradiol. The inhibitory effects of 4-OH-TCB and 17β-estradiol on spontaneous oscillations were counteracted by TEA but were not affected by a calcium ionophore (A23187) or a calcium-dependent potassium channel blocker (apamin). These results suggest that the acute inhibition of spontaneous oscillatory contractions by an estrogenic polychlorinated biphenyl may result from the disruption of intercellular communication. |
| doi_str_mv | 10.1006/taap.1998.8516 |
| format | Article |
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Because increased gap junction formation is associated with the development of synchronized uterine contractions at term, we examined whether the inhibitory effect of 4-OH-TCB on spontaneous oscillatory contractions was due to the disruption of gap junctional communication. The effect of 4-OH-TCB on gap junctional communication was determined by intercellular Lucifer yellow dye transfer in primary cultures of myometrial myocytes isolated from midgestation rats. Intercellular dye transfer was inhibited by 4-OH-TCB or 17β-estradiol in a concentration-dependent manner. The inhibitory effect of 4-OH-TCB on intercellular dye transfer was reversed by tetraethylammonium (TEA). To examine effects on uterine contraction, longitudinal uterine strips were excised from midgestation rats and placed in muscle baths for isometric force measurement. Spontaneous uterine oscillation was suppressed by 4-OH-TCB or 17β-estradiol. The inhibitory effects of 4-OH-TCB and 17β-estradiol on spontaneous oscillations were counteracted by TEA but were not affected by a calcium ionophore (A23187) or a calcium-dependent potassium channel blocker (apamin). These results suggest that the acute inhibition of spontaneous oscillatory contractions by an estrogenic polychlorinated biphenyl may result from the disruption of intercellular communication.</description><identifier>ISSN: 0041-008X</identifier><identifier>EISSN: 1096-0333</identifier><identifier>DOI: 10.1006/taap.1998.8516</identifier><identifier>PMID: 9772196</identifier><identifier>CODEN: TXAPA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Blotting, Western ; Calcimycin - pharmacology ; Cell Communication - drug effects ; Cell Communication - physiology ; Cells, Cultured ; Connexin 43 - analysis ; Dose-Response Relationship, Drug ; Environmental pollutants toxicology ; Estradiol - pharmacology ; Female ; Gap Junctions - drug effects ; Gap Junctions - physiology ; General aspects ; Heptanol - pharmacology ; In Vitro Techniques ; Medical sciences ; Muscle, Smooth - cytology ; Muscle, Smooth - drug effects ; Muscle, Smooth - physiology ; Polychlorinated Biphenyls - pharmacology ; Pregnancy ; Rats ; Rats, Sprague-Dawley ; Tetraethylammonium - pharmacology ; Toxicology ; Uterine Contraction - drug effects ; Uterine Contraction - physiology ; Uterus - cytology ; Uterus - drug effects ; Uterus - physiology</subject><ispartof>Toxicology and applied pharmacology, 1998-09, Vol.152 (1), p.18-29</ispartof><rights>1998 Academic Press</rights><rights>1999 INIST-CNRS</rights><rights>Copyright 1998 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-840e6939a4384f52ab056d1b9bb01e07eb87b65fb9065070003b0a520fafcef53</citedby><cites>FETCH-LOGICAL-c399t-840e6939a4384f52ab056d1b9bb01e07eb87b65fb9065070003b0a520fafcef53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/taap.1998.8516$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1606231$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9772196$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsai, Mei-Ling</creatorcontrib><creatorcontrib>Cesen-Cummings, Kimberley</creatorcontrib><creatorcontrib>Webb, R.Clinton</creatorcontrib><creatorcontrib>Loch-Caruso, Rita</creatorcontrib><title>Acute Inhibition of Spontaneous Uterine Contractions by an Estrogenic Polychlorinated Biphenyl Is Associated with Disruption of Gap Junctional Communication</title><title>Toxicology and applied pharmacology</title><addtitle>Toxicol Appl Pharmacol</addtitle><description>An estrogenic polychlorinated biphenyl, 4-hydroxy-2′,4′,6′-trichlorobiphenyl (4-OH-TCB), inhibits oscillatory uterine contractions immediately. Because increased gap junction formation is associated with the development of synchronized uterine contractions at term, we examined whether the inhibitory effect of 4-OH-TCB on spontaneous oscillatory contractions was due to the disruption of gap junctional communication. The effect of 4-OH-TCB on gap junctional communication was determined by intercellular Lucifer yellow dye transfer in primary cultures of myometrial myocytes isolated from midgestation rats. Intercellular dye transfer was inhibited by 4-OH-TCB or 17β-estradiol in a concentration-dependent manner. The inhibitory effect of 4-OH-TCB on intercellular dye transfer was reversed by tetraethylammonium (TEA). To examine effects on uterine contraction, longitudinal uterine strips were excised from midgestation rats and placed in muscle baths for isometric force measurement. Spontaneous uterine oscillation was suppressed by 4-OH-TCB or 17β-estradiol. The inhibitory effects of 4-OH-TCB and 17β-estradiol on spontaneous oscillations were counteracted by TEA but were not affected by a calcium ionophore (A23187) or a calcium-dependent potassium channel blocker (apamin). These results suggest that the acute inhibition of spontaneous oscillatory contractions by an estrogenic polychlorinated biphenyl may result from the disruption of intercellular communication.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Calcimycin - pharmacology</subject><subject>Cell Communication - drug effects</subject><subject>Cell Communication - physiology</subject><subject>Cells, Cultured</subject><subject>Connexin 43 - analysis</subject><subject>Dose-Response Relationship, Drug</subject><subject>Environmental pollutants toxicology</subject><subject>Estradiol - pharmacology</subject><subject>Female</subject><subject>Gap Junctions - drug effects</subject><subject>Gap Junctions - physiology</subject><subject>General aspects</subject><subject>Heptanol - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Medical sciences</subject><subject>Muscle, Smooth - cytology</subject><subject>Muscle, Smooth - drug effects</subject><subject>Muscle, Smooth - physiology</subject><subject>Polychlorinated Biphenyls - pharmacology</subject><subject>Pregnancy</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Tetraethylammonium - pharmacology</subject><subject>Toxicology</subject><subject>Uterine Contraction - drug effects</subject><subject>Uterine Contraction - physiology</subject><subject>Uterus - cytology</subject><subject>Uterus - drug effects</subject><subject>Uterus - physiology</subject><issn>0041-008X</issn><issn>1096-0333</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-P0zAQxS0EWsrClRuSD4hbyjh_nPhYyrIUrQQSrMTNsp0JNUrtYDugfhc-LM62wAlxsjzzmzej9wh5ymDNAPjLpNS0ZkJ0665h_B5ZMRC8gKqq7pMVQM0KgO7zQ_Ioxq8AIOqaXZAL0bYlE3xFfm7MnJDu3N5qm6x31A_04-RdUg79HOltwmAd0m0uBWUWJFJ9pMrRq5iC_4LOGvrBj0ezH31GVcKevrLTHt1xpLtINzF6Y-_KP2za09c2hnn6vetaTfTd7O6E1ZjXHA5zVlTL_zF5MKgx4pPze0lu31x92r4tbt5f77abm8JUQqSiqwG5qISqq64emlJpaHjPtNAaGEKLums1bwYtgDfQZhcqDaopYVCDwaGpLsmLk-4U_LcZY5IHGw2O48kDyYXIgyX8F2QtY6yu2gyuT6AJPsaAg5yCPahwlAzkkptccpNLbnLJLQ88OyvP-oD9H_wcVO4_P_dVNGocgnLGxr-qHHhZsYx1JwyzXd8tBhmNRWewtwFNkr23_7rgFzpytxo</recordid><startdate>19980901</startdate><enddate>19980901</enddate><creator>Tsai, Mei-Ling</creator><creator>Cesen-Cummings, Kimberley</creator><creator>Webb, R.Clinton</creator><creator>Loch-Caruso, Rita</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19980901</creationdate><title>Acute Inhibition of Spontaneous Uterine Contractions by an Estrogenic Polychlorinated Biphenyl Is Associated with Disruption of Gap Junctional Communication</title><author>Tsai, Mei-Ling ; Cesen-Cummings, Kimberley ; Webb, R.Clinton ; Loch-Caruso, Rita</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-840e6939a4384f52ab056d1b9bb01e07eb87b65fb9065070003b0a520fafcef53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Calcimycin - pharmacology</topic><topic>Cell Communication - drug effects</topic><topic>Cell Communication - physiology</topic><topic>Cells, Cultured</topic><topic>Connexin 43 - analysis</topic><topic>Dose-Response Relationship, Drug</topic><topic>Environmental pollutants toxicology</topic><topic>Estradiol - pharmacology</topic><topic>Female</topic><topic>Gap Junctions - drug effects</topic><topic>Gap Junctions - physiology</topic><topic>General aspects</topic><topic>Heptanol - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Medical sciences</topic><topic>Muscle, Smooth - cytology</topic><topic>Muscle, Smooth - drug effects</topic><topic>Muscle, Smooth - physiology</topic><topic>Polychlorinated Biphenyls - pharmacology</topic><topic>Pregnancy</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Tetraethylammonium - pharmacology</topic><topic>Toxicology</topic><topic>Uterine Contraction - drug effects</topic><topic>Uterine Contraction - physiology</topic><topic>Uterus - cytology</topic><topic>Uterus - drug effects</topic><topic>Uterus - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsai, Mei-Ling</creatorcontrib><creatorcontrib>Cesen-Cummings, Kimberley</creatorcontrib><creatorcontrib>Webb, R.Clinton</creatorcontrib><creatorcontrib>Loch-Caruso, Rita</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Toxicology and applied pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsai, Mei-Ling</au><au>Cesen-Cummings, Kimberley</au><au>Webb, R.Clinton</au><au>Loch-Caruso, Rita</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acute Inhibition of Spontaneous Uterine Contractions by an Estrogenic Polychlorinated Biphenyl Is Associated with Disruption of Gap Junctional Communication</atitle><jtitle>Toxicology and applied pharmacology</jtitle><addtitle>Toxicol Appl Pharmacol</addtitle><date>1998-09-01</date><risdate>1998</risdate><volume>152</volume><issue>1</issue><spage>18</spage><epage>29</epage><pages>18-29</pages><issn>0041-008X</issn><eissn>1096-0333</eissn><coden>TXAPA9</coden><abstract>An estrogenic polychlorinated biphenyl, 4-hydroxy-2′,4′,6′-trichlorobiphenyl (4-OH-TCB), inhibits oscillatory uterine contractions immediately. Because increased gap junction formation is associated with the development of synchronized uterine contractions at term, we examined whether the inhibitory effect of 4-OH-TCB on spontaneous oscillatory contractions was due to the disruption of gap junctional communication. The effect of 4-OH-TCB on gap junctional communication was determined by intercellular Lucifer yellow dye transfer in primary cultures of myometrial myocytes isolated from midgestation rats. Intercellular dye transfer was inhibited by 4-OH-TCB or 17β-estradiol in a concentration-dependent manner. The inhibitory effect of 4-OH-TCB on intercellular dye transfer was reversed by tetraethylammonium (TEA). To examine effects on uterine contraction, longitudinal uterine strips were excised from midgestation rats and placed in muscle baths for isometric force measurement. Spontaneous uterine oscillation was suppressed by 4-OH-TCB or 17β-estradiol. The inhibitory effects of 4-OH-TCB and 17β-estradiol on spontaneous oscillations were counteracted by TEA but were not affected by a calcium ionophore (A23187) or a calcium-dependent potassium channel blocker (apamin). These results suggest that the acute inhibition of spontaneous oscillatory contractions by an estrogenic polychlorinated biphenyl may result from the disruption of intercellular communication.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>9772196</pmid><doi>10.1006/taap.1998.8516</doi><tpages>12</tpages></addata></record> |
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| ispartof | Toxicology and applied pharmacology, 1998-09, Vol.152 (1), p.18-29 |
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| subjects | Animals Biological and medical sciences Blotting, Western Calcimycin - pharmacology Cell Communication - drug effects Cell Communication - physiology Cells, Cultured Connexin 43 - analysis Dose-Response Relationship, Drug Environmental pollutants toxicology Estradiol - pharmacology Female Gap Junctions - drug effects Gap Junctions - physiology General aspects Heptanol - pharmacology In Vitro Techniques Medical sciences Muscle, Smooth - cytology Muscle, Smooth - drug effects Muscle, Smooth - physiology Polychlorinated Biphenyls - pharmacology Pregnancy Rats Rats, Sprague-Dawley Tetraethylammonium - pharmacology Toxicology Uterine Contraction - drug effects Uterine Contraction - physiology Uterus - cytology Uterus - drug effects Uterus - physiology |
| title | Acute Inhibition of Spontaneous Uterine Contractions by an Estrogenic Polychlorinated Biphenyl Is Associated with Disruption of Gap Junctional Communication |
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