essential Schizosaccharomyces pombe cdc23 DNA replication gene shares structural and functional homology with the Saccharomyces cerevisiae DNA43 (MCM10) gene
The fission yeast cdc23 gene is required for correct DNA replication: cdc23 mutants show reduced rates of DNA synthesis and become elongated after cell-cycle arrest. We have cloned the Schizosaccharomyces pombe cdc23 gene by complementation of the temperature-sensitive phenotype of cdc23-M36 and con...
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| Veröffentlicht in: | Current genetics 1998-09, Vol.34 (3), p.164-171 |
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| creator | Aves, S.J Tongue, N Foster, A.J Hart, E.A |
| description | The fission yeast cdc23 gene is required for correct DNA replication: cdc23 mutants show reduced rates of DNA synthesis and become elongated after cell-cycle arrest. We have cloned the Schizosaccharomyces pombe cdc23 gene by complementation of the temperature-sensitive phenotype of cdc23-M36 and confirmed the identity of the gene by integrative mapping. Analysis of the DNA sequence reveals that cdc23 can encode a protein of 593 amino acids (Mr=67 kDa) with 22% overall identity and many structural homologies with the product of the Saccharomyces cerevisiae DNA43 (MCM10) gene which is required for correct initiation of DNA synthesis at chromosomal origins of replication. Construction of a cdc23 null allele has established that the cdc23 gene is essential for viability, with cdc23 deletion mutant spores germinating but undergoing arrest with undivided nuclei in the first or second cell cycle. The S. pombe cdc23 gene on an expression plasmid is able to complement the S. cerevisiae dna43-1 mutant. These structural and functional homologies between two distantly related species suggest that cdc23 and DNA43 may represent genes for a conserved essential eukaryotic DNA replication function. |
| doi_str_mv | 10.1007/s002940050382 |
| format | Article |
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We have cloned the Schizosaccharomyces pombe cdc23 gene by complementation of the temperature-sensitive phenotype of cdc23-M36 and confirmed the identity of the gene by integrative mapping. Analysis of the DNA sequence reveals that cdc23 can encode a protein of 593 amino acids (Mr=67 kDa) with 22% overall identity and many structural homologies with the product of the Saccharomyces cerevisiae DNA43 (MCM10) gene which is required for correct initiation of DNA synthesis at chromosomal origins of replication. Construction of a cdc23 null allele has established that the cdc23 gene is essential for viability, with cdc23 deletion mutant spores germinating but undergoing arrest with undivided nuclei in the first or second cell cycle. The S. pombe cdc23 gene on an expression plasmid is able to complement the S. cerevisiae dna43-1 mutant. 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We have cloned the Schizosaccharomyces pombe cdc23 gene by complementation of the temperature-sensitive phenotype of cdc23-M36 and confirmed the identity of the gene by integrative mapping. Analysis of the DNA sequence reveals that cdc23 can encode a protein of 593 amino acids (Mr=67 kDa) with 22% overall identity and many structural homologies with the product of the Saccharomyces cerevisiae DNA43 (MCM10) gene which is required for correct initiation of DNA synthesis at chromosomal origins of replication. Construction of a cdc23 null allele has established that the cdc23 gene is essential for viability, with cdc23 deletion mutant spores germinating but undergoing arrest with undivided nuclei in the first or second cell cycle. The S. pombe cdc23 gene on an expression plasmid is able to complement the S. cerevisiae dna43-1 mutant. These structural and functional homologies between two distantly related species suggest that cdc23 and DNA43 may represent genes for a conserved essential eukaryotic DNA replication function.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Anaphase-Promoting Complex-Cyclosome</subject><subject>Apc8 Subunit, Anaphase-Promoting Complex-Cyclosome</subject><subject>cdc23 gene</subject><subject>cell cycle</subject><subject>Cell Cycle Proteins - chemistry</subject><subject>Chromosomal Proteins, Non-Histone</subject><subject>Cloning, Molecular</subject><subject>deletions</subject><subject>DNA replication</subject><subject>DNA Replication - genetics</subject><subject>dna replication proteins</subject><subject>DNA-binding proteins</subject><subject>Fungal Proteins - chemistry</subject><subject>genbank/aj224944</subject><subject>Genes, Fungal - genetics</subject><subject>genetic complementation</subject><subject>Genetic Complementation Test</subject><subject>genetic transformation</subject><subject>Microscopy, Fluorescence</subject><subject>Minichromosome Maintenance Proteins</subject><subject>Molecular Sequence Data</subject><subject>mutants</subject><subject>Mutation - genetics</subject><subject>nucleotide sequences</subject><subject>Phenotype</subject><subject>Restriction Mapping</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Schizosaccharomyces - genetics</subject><subject>Schizosaccharomyces pombe</subject><subject>Schizosaccharomyces pombe Proteins</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Deletion - genetics</subject><subject>species differences</subject><subject>structural genes</subject><subject>Ubiquitin-Protein Ligase Complexes</subject><issn>0172-8083</issn><issn>1432-0983</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0U1v1DAQBmALgcpSOHJE-FSVQ2D8FTvHaikfUguHLefIcSYboyRe7AS0_Bf-K152hdQTnKzRPHpH8kvIcwavGYB-kwB4JQEUCMMfkBWTghdQGfGQrIBpXhgw4jF5ktJXAMZNpc_IWaWlAmZW5BemhNPs7UA3rvc_Q7LO9TaGce8w0V0YG6SudVzQt5-uaMTd4J2dfZjoFiekKdvs0hwXNy8xx9ippd0yuYPJYx_GMITtnv7wc0_nHunm3gWHEb_75C0eDkhBL2_Xtwxe_Yl_Sh51dkj47PSek7t313frD8XN5_cf11c3hRNazoXSylomurIVtjNMNEbkTwCulMEOKqtto0XTYtdJbRxjClEZaaCRTLYKxTm5OMbuYvi2YJrr0SeHw2AnDEuqy6oqBRflPyHTTCjJ9X9AqFQpeYbFEboYUorY1bvoRxv3NYP60G99r9_sX5yCl2bE9q8-FZr3L4_7zobabqNP9ZcNByYgN8-1luI32BSp_A</recordid><startdate>19980901</startdate><enddate>19980901</enddate><creator>Aves, S.J</creator><creator>Tongue, N</creator><creator>Foster, A.J</creator><creator>Hart, E.A</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19980901</creationdate><title>essential Schizosaccharomyces pombe cdc23 DNA replication gene shares structural and functional homology with the Saccharomyces cerevisiae DNA43 (MCM10) gene</title><author>Aves, S.J ; Tongue, N ; Foster, A.J ; Hart, E.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c374t-575aa13f6d3af813b8343202558ef09a7ab73bdeff478c115ee58480b414d5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Anaphase-Promoting Complex-Cyclosome</topic><topic>Apc8 Subunit, Anaphase-Promoting Complex-Cyclosome</topic><topic>cdc23 gene</topic><topic>cell cycle</topic><topic>Cell Cycle Proteins - chemistry</topic><topic>Chromosomal Proteins, Non-Histone</topic><topic>Cloning, Molecular</topic><topic>deletions</topic><topic>DNA replication</topic><topic>DNA Replication - genetics</topic><topic>dna replication proteins</topic><topic>DNA-binding proteins</topic><topic>Fungal Proteins - chemistry</topic><topic>genbank/aj224944</topic><topic>Genes, Fungal - genetics</topic><topic>genetic complementation</topic><topic>Genetic Complementation Test</topic><topic>genetic transformation</topic><topic>Microscopy, Fluorescence</topic><topic>Minichromosome Maintenance Proteins</topic><topic>Molecular Sequence Data</topic><topic>mutants</topic><topic>Mutation - genetics</topic><topic>nucleotide sequences</topic><topic>Phenotype</topic><topic>Restriction Mapping</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>Schizosaccharomyces - genetics</topic><topic>Schizosaccharomyces pombe</topic><topic>Schizosaccharomyces pombe Proteins</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Deletion - genetics</topic><topic>species differences</topic><topic>structural genes</topic><topic>Ubiquitin-Protein Ligase Complexes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aves, S.J</creatorcontrib><creatorcontrib>Tongue, N</creatorcontrib><creatorcontrib>Foster, A.J</creatorcontrib><creatorcontrib>Hart, E.A</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Current genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aves, S.J</au><au>Tongue, N</au><au>Foster, A.J</au><au>Hart, E.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>essential Schizosaccharomyces pombe cdc23 DNA replication gene shares structural and functional homology with the Saccharomyces cerevisiae DNA43 (MCM10) gene</atitle><jtitle>Current genetics</jtitle><addtitle>Curr Genet</addtitle><date>1998-09-01</date><risdate>1998</risdate><volume>34</volume><issue>3</issue><spage>164</spage><epage>171</epage><pages>164-171</pages><issn>0172-8083</issn><eissn>1432-0983</eissn><abstract>The fission yeast cdc23 gene is required for correct DNA replication: cdc23 mutants show reduced rates of DNA synthesis and become elongated after cell-cycle arrest. We have cloned the Schizosaccharomyces pombe cdc23 gene by complementation of the temperature-sensitive phenotype of cdc23-M36 and confirmed the identity of the gene by integrative mapping. Analysis of the DNA sequence reveals that cdc23 can encode a protein of 593 amino acids (Mr=67 kDa) with 22% overall identity and many structural homologies with the product of the Saccharomyces cerevisiae DNA43 (MCM10) gene which is required for correct initiation of DNA synthesis at chromosomal origins of replication. Construction of a cdc23 null allele has established that the cdc23 gene is essential for viability, with cdc23 deletion mutant spores germinating but undergoing arrest with undivided nuclei in the first or second cell cycle. The S. pombe cdc23 gene on an expression plasmid is able to complement the S. cerevisiae dna43-1 mutant. These structural and functional homologies between two distantly related species suggest that cdc23 and DNA43 may represent genes for a conserved essential eukaryotic DNA replication function.</abstract><cop>United States</cop><pmid>9745018</pmid><doi>10.1007/s002940050382</doi><tpages>8</tpages></addata></record> |
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| subjects | Amino Acid Sequence amino acid sequences Anaphase-Promoting Complex-Cyclosome Apc8 Subunit, Anaphase-Promoting Complex-Cyclosome cdc23 gene cell cycle Cell Cycle Proteins - chemistry Chromosomal Proteins, Non-Histone Cloning, Molecular deletions DNA replication DNA Replication - genetics dna replication proteins DNA-binding proteins Fungal Proteins - chemistry genbank/aj224944 Genes, Fungal - genetics genetic complementation Genetic Complementation Test genetic transformation Microscopy, Fluorescence Minichromosome Maintenance Proteins Molecular Sequence Data mutants Mutation - genetics nucleotide sequences Phenotype Restriction Mapping Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins Schizosaccharomyces - genetics Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins Sequence Alignment Sequence Analysis, DNA Sequence Deletion - genetics species differences structural genes Ubiquitin-Protein Ligase Complexes |
| title | essential Schizosaccharomyces pombe cdc23 DNA replication gene shares structural and functional homology with the Saccharomyces cerevisiae DNA43 (MCM10) gene |
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