Purification and Characterization of an RNA Dodecamer Sequence Binding Protein from Mitochondria of Saccharomyces cerevisiae

Saccharomyces cerevisiae mitochondrial mRNAs terminate at their 3′ ends with a conserved dodecamer sequence, 5′-AAUAA(U/C)AUUCUU-3′. We have identified a nuclear-encoded protein (DBP) which specifically binds to the dodecamer sequence and have purified it to apparent homogeneity by RNA affinity chro...

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Veröffentlicht in:	Biochemical and biophysical research communications 1999-08, Vol.261 (3), p.740-745
Hauptverfasser:	Li, Huilin, Zassenhaus, H.Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence binding proteins Binding Sites dodecamer binding proteins messenger RNA Mitochondria - chemistry Nucleic Acid Conformation nucleotide sequences Oligonucleotides - chemistry Oligonucleotides - metabolism RNA - chemistry RNA - metabolism RNA-Binding Proteins - isolation & purification RNA-Binding Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - ultrastructure
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