Virulence Markers in the 5′ Untranslated Region of Genotype 2 Bovine Viral Diarrhea Virus Isolates
Virulence markers to distinguish high from low virulence bovine viral diarrhea virus genotype 2 isolates have not been previously reported. The objective of this study was to identify virulence markers by evaluating the primary and secondary structures of the 5′-untranslated region of low and high v...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1998-10, Vol.250 (1), p.164-172 |
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| creator | Topliff, Christina L Kelling, Clayton L |
| description | Virulence markers to distinguish high from low virulence bovine viral diarrhea virus genotype 2 isolates have not been previously reported. The objective of this study was to identify virulence markers by evaluating the primary and secondary structures of the 5′-untranslated region of low and high virulence bovine viral diarrhea virus genotype 2 isolates. The nucleotide sequences of the entire 5′-untranslated region mRNA of eight bovine viral diarrhea virus genotype 2 isolates, four of high virulence and four of low virulence, and two genotype 1 reference isolates were determined using a polymerase chain reaction and a 5′ Rapid Amplification of cDNA Ends System. Two nucleotide substitutions were identified in the internal ribosomal entry site that distinguished the high virulence from the low virulence genotype 2 isolates. The low virulence isolates had a cytosine at position 219, whereas the high virulence isolates had a uracil. At position 278, a uracil or cytosine was found in the low and high virulence groups, respectively. The substituted bases are virulence markers that were used to identify bovine viral diarrhea virus genotype 2 isolates of high virulence. |
| doi_str_mv | 10.1006/viro.1998.9350 |
| format | Article |
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The objective of this study was to identify virulence markers by evaluating the primary and secondary structures of the 5′-untranslated region of low and high virulence bovine viral diarrhea virus genotype 2 isolates. The nucleotide sequences of the entire 5′-untranslated region mRNA of eight bovine viral diarrhea virus genotype 2 isolates, four of high virulence and four of low virulence, and two genotype 1 reference isolates were determined using a polymerase chain reaction and a 5′ Rapid Amplification of cDNA Ends System. Two nucleotide substitutions were identified in the internal ribosomal entry site that distinguished the high virulence from the low virulence genotype 2 isolates. The low virulence isolates had a cytosine at position 219, whereas the high virulence isolates had a uracil. At position 278, a uracil or cytosine was found in the low and high virulence groups, respectively. 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The objective of this study was to identify virulence markers by evaluating the primary and secondary structures of the 5′-untranslated region of low and high virulence bovine viral diarrhea virus genotype 2 isolates. The nucleotide sequences of the entire 5′-untranslated region mRNA of eight bovine viral diarrhea virus genotype 2 isolates, four of high virulence and four of low virulence, and two genotype 1 reference isolates were determined using a polymerase chain reaction and a 5′ Rapid Amplification of cDNA Ends System. Two nucleotide substitutions were identified in the internal ribosomal entry site that distinguished the high virulence from the low virulence genotype 2 isolates. The low virulence isolates had a cytosine at position 219, whereas the high virulence isolates had a uracil. At position 278, a uracil or cytosine was found in the low and high virulence groups, respectively. 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The objective of this study was to identify virulence markers by evaluating the primary and secondary structures of the 5′-untranslated region of low and high virulence bovine viral diarrhea virus genotype 2 isolates. The nucleotide sequences of the entire 5′-untranslated region mRNA of eight bovine viral diarrhea virus genotype 2 isolates, four of high virulence and four of low virulence, and two genotype 1 reference isolates were determined using a polymerase chain reaction and a 5′ Rapid Amplification of cDNA Ends System. Two nucleotide substitutions were identified in the internal ribosomal entry site that distinguished the high virulence from the low virulence genotype 2 isolates. The low virulence isolates had a cytosine at position 219, whereas the high virulence isolates had a uracil. At position 278, a uracil or cytosine was found in the low and high virulence groups, respectively. The substituted bases are virulence markers that were used to identify bovine viral diarrhea virus genotype 2 isolates of high virulence.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>9770430</pmid><doi>10.1006/viro.1998.9350</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Virology (New York, N.Y.), 1998-10, Vol.250 (1), p.164-172 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals |
| subjects | 5' Untranslated Regions - genetics Animals Base Sequence BOVINE DIARRHOEA PESTIVIRUS Bovine viral diarrhea virus-2 Bovine Virus Diarrhea-Mucosal Disease - virology Cattle Diarrhea Viruses, Bovine Viral - genetics Diarrhea Viruses, Bovine Viral - pathogenicity DNA, Complementary GENETIC MARKERS Genetic Variation Genotype Molecular Sequence Data Nucleic Acid Conformation PATHOGENICITY Phylogeny RNA, Viral Sequence Analysis, DNA Sequence Homology, Nucleic Acid Virulence |
| title | Virulence Markers in the 5′ Untranslated Region of Genotype 2 Bovine Viral Diarrhea Virus Isolates |
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