Quantifying biosynthetic human growth hormone in Escherichia coli with capillary electrophoresis under hydrophobic conditions
A method has been developed which is able to quantitate the content of precursor biosynthetic human growth hormone (Pre-bhGH) in the cytosol of E. coli cells containing the gene for human growth hormone (hGH). The method uses hydrophobic C 18 coated capillaries with native biosynthetic human growth...
Gespeichert in:
| Veröffentlicht in: | Journal of Chromatography A 1998-08, Vol.817 (1), p.205-214 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 214 |
|---|---|
| container_issue | 1 |
| container_start_page | 205 |
| container_title | Journal of Chromatography A |
| container_volume | 817 |
| creator | Jørgensen, Thomas K Bagger, Leif H Christiansen, Jesper Johnsen, Gitte H Faarbæk, Jan R Jørgensen, Linda Welinder, Benny S |
| description | A method has been developed which is able to quantitate the content of precursor biosynthetic human growth hormone (Pre-bhGH) in the cytosol of
E.
coli cells containing the gene for human growth hormone (hGH). The method uses hydrophobic C
18 coated capillaries with native biosynthetic human growth hormone (bhGH) as an internal standard. This allows for highly robust and precise determinations as well as the evaluation of the presence of deamidated forms in the cytosol samples. Furthermore, by modifying the running buffer with zwitterionic surfactants and an organic modifier, it is possible to detect a related form with a three sulfur atom Cys–Cys bridge (trisulfide Pre-bhGH). Thus, a strong tool for monitoring the effect of fermentation conditions on the biosynthesis of bhGH is obtained. |
| doi_str_mv | 10.1016/S0021-9673(98)00424-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69955262</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0021967398004245</els_id><sourcerecordid>69955262</sourcerecordid><originalsourceid>FETCH-LOGICAL-c389t-ee49c4be19e6a159116625271a0d2a615a27c9d9c466103ac029d74315c673f63</originalsourceid><addsrcrecordid>eNqFkEtv1DAQgH0AlVL4CZV8QAgOobYTO-sTQlV5SJWqqnC2vJNJMyixFztptQf-O96H9spppJlvXh9jl1J8kkKaqwchlKysaesPdvVRiEY1lX7Bzk_pV-x1zr-FkK1o1Rk7s61pGqvP2d_7xYeZ-i2FR76mmLdhHnAm4MMy-cAfU3yeBz7ENMWAnAK_yTBgIhjIc4gj8WcqAPgNjaNPW44jwpziprRgpsyX0GHiw7bb59ZlMsTQ0Uwx5DfsZe_HjG-P8YL9-nrz8_p7dXv37cf1l9sK6pWdK8TGQrNGadF4qa2UxiitWulFp7yR2qsWbFcYY6SoPQhlu7appYbye2_qC_b-MHeT4p8F8-wmyoDl4IBxyc5Yq7UyqoD6AEKKOSfs3SbRVN5yUridardX7XZOnV25vWqnS9_lccGynrA7dR09l_q7Y91n8GOffADKJ0w19UqJ3frPBwyLjCfC5DIQBsCOUrHqukj_OeQfmdWfkw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69955262</pqid></control><display><type>article</type><title>Quantifying biosynthetic human growth hormone in Escherichia coli with capillary electrophoresis under hydrophobic conditions</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Jørgensen, Thomas K ; Bagger, Leif H ; Christiansen, Jesper ; Johnsen, Gitte H ; Faarbæk, Jan R ; Jørgensen, Linda ; Welinder, Benny S</creator><creatorcontrib>Jørgensen, Thomas K ; Bagger, Leif H ; Christiansen, Jesper ; Johnsen, Gitte H ; Faarbæk, Jan R ; Jørgensen, Linda ; Welinder, Benny S</creatorcontrib><description>A method has been developed which is able to quantitate the content of precursor biosynthetic human growth hormone (Pre-bhGH) in the cytosol of
E.
coli cells containing the gene for human growth hormone (hGH). The method uses hydrophobic C
18 coated capillaries with native biosynthetic human growth hormone (bhGH) as an internal standard. This allows for highly robust and precise determinations as well as the evaluation of the presence of deamidated forms in the cytosol samples. Furthermore, by modifying the running buffer with zwitterionic surfactants and an organic modifier, it is possible to detect a related form with a three sulfur atom Cys–Cys bridge (trisulfide Pre-bhGH). Thus, a strong tool for monitoring the effect of fermentation conditions on the biosynthesis of bhGH is obtained.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/S0021-9673(98)00424-5</identifier><identifier>PMID: 9764495</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Biological and medical sciences ; Biotechnology ; Electrophoresis, Capillary - methods ; Escherichia coli - chemistry ; Fundamental and applied biological sciences. Psychology ; Growth hormones ; Health. Pharmaceutical industry ; Hormones. Growth factors ; Human Growth Hormone - analysis ; Humans ; Industrial applications and implications. Economical aspects ; Molecular Sequence Data ; Production of active biomolecules ; Protein Precursors - analysis ; Recombinant Proteins - analysis ; Reference Standards ; Reproducibility of Results</subject><ispartof>Journal of Chromatography A, 1998-08, Vol.817 (1), p.205-214</ispartof><rights>1998 Elsevier Science B.V.</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-ee49c4be19e6a159116625271a0d2a615a27c9d9c466103ac029d74315c673f63</citedby><cites>FETCH-LOGICAL-c389t-ee49c4be19e6a159116625271a0d2a615a27c9d9c466103ac029d74315c673f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0021967398004245$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,3536,23910,23911,25119,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2438202$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9764495$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jørgensen, Thomas K</creatorcontrib><creatorcontrib>Bagger, Leif H</creatorcontrib><creatorcontrib>Christiansen, Jesper</creatorcontrib><creatorcontrib>Johnsen, Gitte H</creatorcontrib><creatorcontrib>Faarbæk, Jan R</creatorcontrib><creatorcontrib>Jørgensen, Linda</creatorcontrib><creatorcontrib>Welinder, Benny S</creatorcontrib><title>Quantifying biosynthetic human growth hormone in Escherichia coli with capillary electrophoresis under hydrophobic conditions</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>A method has been developed which is able to quantitate the content of precursor biosynthetic human growth hormone (Pre-bhGH) in the cytosol of
E.
coli cells containing the gene for human growth hormone (hGH). The method uses hydrophobic C
18 coated capillaries with native biosynthetic human growth hormone (bhGH) as an internal standard. This allows for highly robust and precise determinations as well as the evaluation of the presence of deamidated forms in the cytosol samples. Furthermore, by modifying the running buffer with zwitterionic surfactants and an organic modifier, it is possible to detect a related form with a three sulfur atom Cys–Cys bridge (trisulfide Pre-bhGH). Thus, a strong tool for monitoring the effect of fermentation conditions on the biosynthesis of bhGH is obtained.</description><subject>Amino Acid Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Escherichia coli - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth hormones</subject><subject>Health. Pharmaceutical industry</subject><subject>Hormones. Growth factors</subject><subject>Human Growth Hormone - analysis</subject><subject>Humans</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Molecular Sequence Data</subject><subject>Production of active biomolecules</subject><subject>Protein Precursors - analysis</subject><subject>Recombinant Proteins - analysis</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv1DAQgH0AlVL4CZV8QAgOobYTO-sTQlV5SJWqqnC2vJNJMyixFztptQf-O96H9spppJlvXh9jl1J8kkKaqwchlKysaesPdvVRiEY1lX7Bzk_pV-x1zr-FkK1o1Rk7s61pGqvP2d_7xYeZ-i2FR76mmLdhHnAm4MMy-cAfU3yeBz7ENMWAnAK_yTBgIhjIc4gj8WcqAPgNjaNPW44jwpziprRgpsyX0GHiw7bb59ZlMsTQ0Uwx5DfsZe_HjG-P8YL9-nrz8_p7dXv37cf1l9sK6pWdK8TGQrNGadF4qa2UxiitWulFp7yR2qsWbFcYY6SoPQhlu7appYbye2_qC_b-MHeT4p8F8-wmyoDl4IBxyc5Yq7UyqoD6AEKKOSfs3SbRVN5yUridardX7XZOnV25vWqnS9_lccGynrA7dR09l_q7Y91n8GOffADKJ0w19UqJ3frPBwyLjCfC5DIQBsCOUrHqukj_OeQfmdWfkw</recordid><startdate>19980821</startdate><enddate>19980821</enddate><creator>Jørgensen, Thomas K</creator><creator>Bagger, Leif H</creator><creator>Christiansen, Jesper</creator><creator>Johnsen, Gitte H</creator><creator>Faarbæk, Jan R</creator><creator>Jørgensen, Linda</creator><creator>Welinder, Benny S</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980821</creationdate><title>Quantifying biosynthetic human growth hormone in Escherichia coli with capillary electrophoresis under hydrophobic conditions</title><author>Jørgensen, Thomas K ; Bagger, Leif H ; Christiansen, Jesper ; Johnsen, Gitte H ; Faarbæk, Jan R ; Jørgensen, Linda ; Welinder, Benny S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-ee49c4be19e6a159116625271a0d2a615a27c9d9c466103ac029d74315c673f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Amino Acid Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Escherichia coli - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth hormones</topic><topic>Health. Pharmaceutical industry</topic><topic>Hormones. Growth factors</topic><topic>Human Growth Hormone - analysis</topic><topic>Humans</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Molecular Sequence Data</topic><topic>Production of active biomolecules</topic><topic>Protein Precursors - analysis</topic><topic>Recombinant Proteins - analysis</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jørgensen, Thomas K</creatorcontrib><creatorcontrib>Bagger, Leif H</creatorcontrib><creatorcontrib>Christiansen, Jesper</creatorcontrib><creatorcontrib>Johnsen, Gitte H</creatorcontrib><creatorcontrib>Faarbæk, Jan R</creatorcontrib><creatorcontrib>Jørgensen, Linda</creatorcontrib><creatorcontrib>Welinder, Benny S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jørgensen, Thomas K</au><au>Bagger, Leif H</au><au>Christiansen, Jesper</au><au>Johnsen, Gitte H</au><au>Faarbæk, Jan R</au><au>Jørgensen, Linda</au><au>Welinder, Benny S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantifying biosynthetic human growth hormone in Escherichia coli with capillary electrophoresis under hydrophobic conditions</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>1998-08-21</date><risdate>1998</risdate><volume>817</volume><issue>1</issue><spage>205</spage><epage>214</epage><pages>205-214</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>A method has been developed which is able to quantitate the content of precursor biosynthetic human growth hormone (Pre-bhGH) in the cytosol of
E.
coli cells containing the gene for human growth hormone (hGH). The method uses hydrophobic C
18 coated capillaries with native biosynthetic human growth hormone (bhGH) as an internal standard. This allows for highly robust and precise determinations as well as the evaluation of the presence of deamidated forms in the cytosol samples. Furthermore, by modifying the running buffer with zwitterionic surfactants and an organic modifier, it is possible to detect a related form with a three sulfur atom Cys–Cys bridge (trisulfide Pre-bhGH). Thus, a strong tool for monitoring the effect of fermentation conditions on the biosynthesis of bhGH is obtained.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>9764495</pmid><doi>10.1016/S0021-9673(98)00424-5</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9673 |
| ispartof | Journal of Chromatography A, 1998-08, Vol.817 (1), p.205-214 |
| issn | 0021-9673 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69955262 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Biological and medical sciences Biotechnology Electrophoresis, Capillary - methods Escherichia coli - chemistry Fundamental and applied biological sciences. Psychology Growth hormones Health. Pharmaceutical industry Hormones. Growth factors Human Growth Hormone - analysis Humans Industrial applications and implications. Economical aspects Molecular Sequence Data Production of active biomolecules Protein Precursors - analysis Recombinant Proteins - analysis Reference Standards Reproducibility of Results |
| title | Quantifying biosynthetic human growth hormone in Escherichia coli with capillary electrophoresis under hydrophobic conditions |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T07%3A19%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantifying%20biosynthetic%20human%20growth%20hormone%20in%20Escherichia%20coli%20with%20capillary%20electrophoresis%20under%20hydrophobic%20conditions&rft.jtitle=Journal%20of%20Chromatography%20A&rft.au=J%C3%B8rgensen,%20Thomas%20K&rft.date=1998-08-21&rft.volume=817&rft.issue=1&rft.spage=205&rft.epage=214&rft.pages=205-214&rft.issn=0021-9673&rft.coden=JOCRAM&rft_id=info:doi/10.1016/S0021-9673(98)00424-5&rft_dat=%3Cproquest_cross%3E69955262%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69955262&rft_id=info:pmid/9764495&rft_els_id=S0021967398004245&rfr_iscdi=true |