Localization of C. trachomatis inc proteins in expression of their genes in HeLa cell culture

Co-localization of Chlamydia trachomatis incorporation membrane proteins with cell organelles was studied in HeLa cell culture after transfection by expressing vectors carrying incA, incB, incC, incD, incE, incF, incG genes, respectively, fused with the marker green fluorescent protein (EGFP) gene....

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Veröffentlicht in:	Bulletin of experimental biology and medicine 2008-08, Vol.146 (2), p.237-242
Hauptverfasser:	Shkarupeta, M. M, Kostrjukova, E. S, Lazarev, V. N, Levitskii, S. A, Basovskii, Yu. I, Govorun, V. M
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - metabolism Biomedical and Life Sciences Biomedicine Cell Biology Chlamydia trachomatis Chlamydia trachomatis - genetics Gene Expression Genes, Bacterial Genetics Green Fluorescent Proteins HeLa Cells Humans IncA IncB IncC IncD IncE IncF IncG incorporation membrane proteins Internal Medicine Laboratory Medicine Membrane Proteins - metabolism Microscopy, Confocal Microscopy, Fluorescence Organelles - metabolism Pathology Recombinant Fusion Proteins - metabolism Transfection
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Zusammenfassung:	Co-localization of Chlamydia trachomatis incorporation membrane proteins with cell organelles was studied in HeLa cell culture after transfection by expressing vectors carrying incA, incB, incC, incD, incE, incF, incG genes, respectively, fused with the marker green fluorescent protein (EGFP) gene. The prokaryotic proteins were co-located with compartments of the secretory pathway of the eukaryotic cell in the course of biogenesis.
ISSN:	0007-4888 1573-8221
DOI:	10.1007/s10517-008-0254-8