Synthesis, cloning and expression of the single-chain Fv gene of the HPr-specific monoclonal antibody, Jel42. Determination of binding constants with wild-type and mutant HPrs

The monoclonal antibody Jel42 is specific for the Escherichia coli histidine-containing protein, HPr, which is an 85 amino acid phosphocarrier protein of the phosphoenolpyruvate:sugar phosphotransferase system. The binding domain (Fv) has been produced as a single chain Fv (scFv). The scFv gene was...

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Veröffentlicht in:	Protein engineering 1999-07, Vol.12 (7), p.623-630
Hauptverfasser:	Smallshaw, Joan E., Georges, Fawzy, Lee, Jeremy S., Waygood, E.Bruce
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Antibodies, Monoclonal - genetics Antibodies, Monoclonal - metabolism antibody Antigen-Antibody Complex Bacterial Proteins Base Sequence Cloning, Molecular Escherichia coli gene synthesis HPr HPr protein Immunoglobulin Variable Region - genetics Immunoglobulin Variable Region - isolation & purification Immunoglobulin Variable Region - metabolism Molecular Sequence Data Mutation Phosphoenolpyruvate Sugar Phosphotransferase System - genetics Phosphoenolpyruvate Sugar Phosphotransferase System - immunology phosphoenolpyruvate:sugar phosphotransferase protein binding constant Protein Folding Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Sequence Analysis, DNA single-chain Fv Temperature
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