Improved immunofluorescence assay for detection of Giardia and Cryptosporidium from asymptomatic adult cervine animals
We tested an improved immunofluorescence assay (IFA) in detecting Giardia and Cryptosporidium from feces of asymptomatic adult cervine animals. Samples were concentrated by sucrose flotation before being stained by fluorescent monoclonal antibody and examined microscopically. The detection limit was...
Gespeichert in:
| Veröffentlicht in: | Parasitology research (1987) 1999-08, Vol.85 (8-9), p.733-736 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 736 |
|---|---|
| container_issue | 8-9 |
| container_start_page | 733 |
| container_title | Parasitology research (1987) |
| container_volume | 85 |
| creator | DENG, M. Q CLIVER, D. O |
| description | We tested an improved immunofluorescence assay (IFA) in detecting Giardia and Cryptosporidium from feces of asymptomatic adult cervine animals. Samples were concentrated by sucrose flotation before being stained by fluorescent monoclonal antibody and examined microscopically. The detection limit was determined as 500 G. intestinalis cysts or 200 C. parvum oocysts/g of sample. Among the 82 samples collected from adult fallow deer, Columbian black-tailed deer, and Tule elk in northern California, 3 (3.7%) contained G. intestinalis cysts, which were confirmed by a species-specific polymerase chain reaction (PCR) following immunomagnetic capture (IC) of cysts. C. parvum oocysts were detected in a total of 13 (15.9%) samples, and oocysts from 2 such samples were smaller than oocysts from the other 11 samples. C. parvum identification was also confirmed by specific IC-PCR and sequencing of the PCR product. In addition, a C. muris-like organism was detected in 2 (2.4%) samples. Findings obtained with the improved IFA confirmed that cysts/oocysts may pass unnoticed in adult cervine animals and that subclinically infected individuals could serve as potential carriers of infection for humans and other animals via contaminated feces or water. |
| doi_str_mv | 10.1007/s004360050623 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69935634</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69935634</sourcerecordid><originalsourceid>FETCH-LOGICAL-c318t-4bcf607344ef162ed9fe518a1cc54ed3871b96432d757c2195f96ade17b842033</originalsourceid><addsrcrecordid>eNpVkM1LxDAQxYMoun4cvUoO4q2aadKkPcriFwhe9FyyyQQiTbMm7cL-90Z2QT3NMPzeY94j5BLYLTCm7jJjgkvGGiZrfkAWIHhdQdc0h2TBurIzAH5CTnP-ZAyUFOKYnEDRgBKwIJuXsE5xg5b6EOYxumGOCbPB0SDVOestdTFRixOayceRRkefvE7Wa6pHS5dpu55iXsfkrZ8DdSmGotuGcg168oZqOw8TNZg2fiyWow96yOfkyJWBF_t5Rj4eH96Xz9Xr29PL8v61MhzaqRIr4yRTXAh0IGu0ncMGWg3GNAItbxWsOlkSW9UoU5fYrpPaIqhVK2rG-Rm52fmWkF8z5qkPvoQbBj1inHMvu443kosCVjvQpJhzQtevU_k0bXtg_U_R_b-iC3-1N55XAe0fetdsAa73gM5GDy7p0fj8y7VKSg78G3xFh1I</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69935634</pqid></control><display><type>article</type><title>Improved immunofluorescence assay for detection of Giardia and Cryptosporidium from asymptomatic adult cervine animals</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>DENG, M. Q ; CLIVER, D. O</creator><creatorcontrib>DENG, M. Q ; CLIVER, D. O</creatorcontrib><description>We tested an improved immunofluorescence assay (IFA) in detecting Giardia and Cryptosporidium from feces of asymptomatic adult cervine animals. Samples were concentrated by sucrose flotation before being stained by fluorescent monoclonal antibody and examined microscopically. The detection limit was determined as 500 G. intestinalis cysts or 200 C. parvum oocysts/g of sample. Among the 82 samples collected from adult fallow deer, Columbian black-tailed deer, and Tule elk in northern California, 3 (3.7%) contained G. intestinalis cysts, which were confirmed by a species-specific polymerase chain reaction (PCR) following immunomagnetic capture (IC) of cysts. C. parvum oocysts were detected in a total of 13 (15.9%) samples, and oocysts from 2 such samples were smaller than oocysts from the other 11 samples. C. parvum identification was also confirmed by specific IC-PCR and sequencing of the PCR product. In addition, a C. muris-like organism was detected in 2 (2.4%) samples. Findings obtained with the improved IFA confirmed that cysts/oocysts may pass unnoticed in adult cervine animals and that subclinically infected individuals could serve as potential carriers of infection for humans and other animals via contaminated feces or water.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s004360050623</identifier><identifier>PMID: 10431741</identifier><identifier>CODEN: PARREZ</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animal protozoal diseases ; Animals ; Biological and medical sciences ; Cryptosporidiosis - parasitology ; Cryptosporidiosis - veterinary ; Cryptosporidium parvum - genetics ; Cryptosporidium parvum - immunology ; Cryptosporidium parvum - isolation & purification ; Deer ; DNA, Protozoan - analysis ; Giardia lamblia - genetics ; Giardia lamblia - immunology ; Giardia lamblia - isolation & purification ; Giardiasis - parasitology ; Giardiasis - veterinary ; Infectious diseases ; Medical sciences ; Microscopy, Fluorescence - methods ; Parasitic diseases ; Polymerase Chain Reaction ; Protozoal diseases ; Sequence Analysis, DNA</subject><ispartof>Parasitology research (1987), 1999-08, Vol.85 (8-9), p.733-736</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c318t-4bcf607344ef162ed9fe518a1cc54ed3871b96432d757c2195f96ade17b842033</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1876631$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10431741$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DENG, M. Q</creatorcontrib><creatorcontrib>CLIVER, D. O</creatorcontrib><title>Improved immunofluorescence assay for detection of Giardia and Cryptosporidium from asymptomatic adult cervine animals</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><description>We tested an improved immunofluorescence assay (IFA) in detecting Giardia and Cryptosporidium from feces of asymptomatic adult cervine animals. Samples were concentrated by sucrose flotation before being stained by fluorescent monoclonal antibody and examined microscopically. The detection limit was determined as 500 G. intestinalis cysts or 200 C. parvum oocysts/g of sample. Among the 82 samples collected from adult fallow deer, Columbian black-tailed deer, and Tule elk in northern California, 3 (3.7%) contained G. intestinalis cysts, which were confirmed by a species-specific polymerase chain reaction (PCR) following immunomagnetic capture (IC) of cysts. C. parvum oocysts were detected in a total of 13 (15.9%) samples, and oocysts from 2 such samples were smaller than oocysts from the other 11 samples. C. parvum identification was also confirmed by specific IC-PCR and sequencing of the PCR product. In addition, a C. muris-like organism was detected in 2 (2.4%) samples. Findings obtained with the improved IFA confirmed that cysts/oocysts may pass unnoticed in adult cervine animals and that subclinically infected individuals could serve as potential carriers of infection for humans and other animals via contaminated feces or water.</description><subject>Animal protozoal diseases</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cryptosporidiosis - parasitology</subject><subject>Cryptosporidiosis - veterinary</subject><subject>Cryptosporidium parvum - genetics</subject><subject>Cryptosporidium parvum - immunology</subject><subject>Cryptosporidium parvum - isolation & purification</subject><subject>Deer</subject><subject>DNA, Protozoan - analysis</subject><subject>Giardia lamblia - genetics</subject><subject>Giardia lamblia - immunology</subject><subject>Giardia lamblia - isolation & purification</subject><subject>Giardiasis - parasitology</subject><subject>Giardiasis - veterinary</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Microscopy, Fluorescence - methods</subject><subject>Parasitic diseases</subject><subject>Polymerase Chain Reaction</subject><subject>Protozoal diseases</subject><subject>Sequence Analysis, DNA</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM1LxDAQxYMoun4cvUoO4q2aadKkPcriFwhe9FyyyQQiTbMm7cL-90Z2QT3NMPzeY94j5BLYLTCm7jJjgkvGGiZrfkAWIHhdQdc0h2TBurIzAH5CTnP-ZAyUFOKYnEDRgBKwIJuXsE5xg5b6EOYxumGOCbPB0SDVOestdTFRixOayceRRkefvE7Wa6pHS5dpu55iXsfkrZ8DdSmGotuGcg168oZqOw8TNZg2fiyWow96yOfkyJWBF_t5Rj4eH96Xz9Xr29PL8v61MhzaqRIr4yRTXAh0IGu0ncMGWg3GNAItbxWsOlkSW9UoU5fYrpPaIqhVK2rG-Rm52fmWkF8z5qkPvoQbBj1inHMvu443kosCVjvQpJhzQtevU_k0bXtg_U_R_b-iC3-1N55XAe0fetdsAa73gM5GDy7p0fj8y7VKSg78G3xFh1I</recordid><startdate>19990801</startdate><enddate>19990801</enddate><creator>DENG, M. Q</creator><creator>CLIVER, D. O</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990801</creationdate><title>Improved immunofluorescence assay for detection of Giardia and Cryptosporidium from asymptomatic adult cervine animals</title><author>DENG, M. Q ; CLIVER, D. O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c318t-4bcf607344ef162ed9fe518a1cc54ed3871b96432d757c2195f96ade17b842033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animal protozoal diseases</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cryptosporidiosis - parasitology</topic><topic>Cryptosporidiosis - veterinary</topic><topic>Cryptosporidium parvum - genetics</topic><topic>Cryptosporidium parvum - immunology</topic><topic>Cryptosporidium parvum - isolation & purification</topic><topic>Deer</topic><topic>DNA, Protozoan - analysis</topic><topic>Giardia lamblia - genetics</topic><topic>Giardia lamblia - immunology</topic><topic>Giardia lamblia - isolation & purification</topic><topic>Giardiasis - parasitology</topic><topic>Giardiasis - veterinary</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Microscopy, Fluorescence - methods</topic><topic>Parasitic diseases</topic><topic>Polymerase Chain Reaction</topic><topic>Protozoal diseases</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DENG, M. Q</creatorcontrib><creatorcontrib>CLIVER, D. O</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DENG, M. Q</au><au>CLIVER, D. O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved immunofluorescence assay for detection of Giardia and Cryptosporidium from asymptomatic adult cervine animals</atitle><jtitle>Parasitology research (1987)</jtitle><addtitle>Parasitol Res</addtitle><date>1999-08-01</date><risdate>1999</risdate><volume>85</volume><issue>8-9</issue><spage>733</spage><epage>736</epage><pages>733-736</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><coden>PARREZ</coden><abstract>We tested an improved immunofluorescence assay (IFA) in detecting Giardia and Cryptosporidium from feces of asymptomatic adult cervine animals. Samples were concentrated by sucrose flotation before being stained by fluorescent monoclonal antibody and examined microscopically. The detection limit was determined as 500 G. intestinalis cysts or 200 C. parvum oocysts/g of sample. Among the 82 samples collected from adult fallow deer, Columbian black-tailed deer, and Tule elk in northern California, 3 (3.7%) contained G. intestinalis cysts, which were confirmed by a species-specific polymerase chain reaction (PCR) following immunomagnetic capture (IC) of cysts. C. parvum oocysts were detected in a total of 13 (15.9%) samples, and oocysts from 2 such samples were smaller than oocysts from the other 11 samples. C. parvum identification was also confirmed by specific IC-PCR and sequencing of the PCR product. In addition, a C. muris-like organism was detected in 2 (2.4%) samples. Findings obtained with the improved IFA confirmed that cysts/oocysts may pass unnoticed in adult cervine animals and that subclinically infected individuals could serve as potential carriers of infection for humans and other animals via contaminated feces or water.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>10431741</pmid><doi>10.1007/s004360050623</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0932-0113 |
| ispartof | Parasitology research (1987), 1999-08, Vol.85 (8-9), p.733-736 |
| issn | 0932-0113 1432-1955 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69935634 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animal protozoal diseases Animals Biological and medical sciences Cryptosporidiosis - parasitology Cryptosporidiosis - veterinary Cryptosporidium parvum - genetics Cryptosporidium parvum - immunology Cryptosporidium parvum - isolation & purification Deer DNA, Protozoan - analysis Giardia lamblia - genetics Giardia lamblia - immunology Giardia lamblia - isolation & purification Giardiasis - parasitology Giardiasis - veterinary Infectious diseases Medical sciences Microscopy, Fluorescence - methods Parasitic diseases Polymerase Chain Reaction Protozoal diseases Sequence Analysis, DNA |
| title | Improved immunofluorescence assay for detection of Giardia and Cryptosporidium from asymptomatic adult cervine animals |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T03%3A01%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Improved%20immunofluorescence%20assay%20for%20detection%20of%20Giardia%20and%20Cryptosporidium%20from%20asymptomatic%20adult%20cervine%20animals&rft.jtitle=Parasitology%20research%20(1987)&rft.au=DENG,%20M.%20Q&rft.date=1999-08-01&rft.volume=85&rft.issue=8-9&rft.spage=733&rft.epage=736&rft.pages=733-736&rft.issn=0932-0113&rft.eissn=1432-1955&rft.coden=PARREZ&rft_id=info:doi/10.1007/s004360050623&rft_dat=%3Cproquest_cross%3E69935634%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69935634&rft_id=info:pmid/10431741&rfr_iscdi=true |