Membrane perturbing factor in reticulocyte lysate, which is transiently activated by proteases
Proteases have been used to examine the topology of proteins on various membranes. We reexamined the conditions of protease treatment for rough microsomal membranes and found that proteinase K degraded the lumenal proteins in the presence of reticulocyte lysate. The lysate treated with either heat o...
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| Veröffentlicht in: | FEBS letters 1999-07, Vol.454 (3), p.345-348 |
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| creator | Sakaki, Kenjiro Sakaguchi, Masao Ota, Kazuhisa Mihara, Katsuyoshi |
| description | Proteases have been used to examine the topology of proteins on various membranes. We reexamined the conditions of protease treatment for rough microsomal membranes and found that proteinase K degraded the lumenal proteins in the presence of reticulocyte lysate. The lysate treated with either heat or
N-ethylmaleimide no longer promoted the degradation. The reticulocyte dependent degradation was also observed with papain, trypsin, and elastase. This activity was transiently generated by treating reticulocyte lysate short-term with trypsin. We thus concluded that a membrane perturbing factor(s) must exist in reticulocyte which is transiently activated by protease treatment. |
| doi_str_mv | 10.1016/S0014-5793(99)00842-X |
| format | Article |
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| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1999-07, Vol.454 (3), p.345-348 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; Alma/SFX Local Collection |
| subjects | Animals Dogs Endopeptidase K - metabolism Membrane Proteins - metabolism Membrane topology Microsomes - metabolism mPL, mature prolactin PDI protein disulfide isomerase pPL, preprolactin: RL, reticulocyte lysate Protease treatment: Membrane degradation Rabbits Reticulocytes - metabolism RM, rough microsomal membrane Substrate Specificity |
| title | Membrane perturbing factor in reticulocyte lysate, which is transiently activated by proteases |
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