Annual changes in serum leptin concentration in the adult female Japanese black bear (Ursus thibetanus japonicus)
In the present study, assay of the serum leptin concentration of the Japanese black bear (Ursus thibetanus japonicus) was attempted using a canine-leptin-specific sandwich enzyme-linked immunosorbent assay (ELISA). The dose-response curve of the bear serum was linear and parallel to the canine lepti...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2008, Vol.70(12), pp.1399-1403 |
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| container_title | Journal of Veterinary Medical Science |
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| creator | Tsubota, T.(Hokkaido Univ., Sapporo (Japan)) Sato, M Okano, T Nakamura, S Asano, M Komatsu, T Shibata, H Saito, M |
| description | In the present study, assay of the serum leptin concentration of the Japanese black bear (Ursus thibetanus japonicus) was attempted using a canine-leptin-specific sandwich enzyme-linked immunosorbent assay (ELISA). The dose-response curve of the bear serum was linear and parallel to the canine leptin standard curve. In mated and unmated bears, the serum leptin concentration was stable at low levels from May to August or September, gradually increased from September or October, and then remarkably increased in late November. We conclude that this method may be useful for measuring bear serum leptin concentration and that the serum leptin concentration changes annually with a peak in late November. |
| doi_str_mv | 10.1292/jvms.70.1399 |
| format | Article |
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The dose-response curve of the bear serum was linear and parallel to the canine leptin standard curve. In mated and unmated bears, the serum leptin concentration was stable at low levels from May to August or September, gradually increased from September or October, and then remarkably increased in late November. We conclude that this method may be useful for measuring bear serum leptin concentration and that the serum leptin concentration changes annually with a peak in late November.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.70.1399</identifier><identifier>PMID: 19122415</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Animals ; Animals, Zoo ; BLOOD PROTEINS ; ELISA ; Female ; GAIN DE POIDS ; GANANCIA DE PESO ; Japanese black bear ; leptin ; Leptin - blood ; PROTEINAS SANGUINEAS ; PROTEINE SANGUINE ; SEASONAL VARIATION ; TEST ELISA ; Time Factors ; URSIDAE ; Ursidae - physiology ; Ursus thibetanus japonicus ; VARIACION ESTACIONAL ; VARIATION SAISONNIERE ; WEIGHT GAIN</subject><ispartof>Journal of Veterinary Medical Science, 2008, Vol.70(12), pp.1399-1403</ispartof><rights>2008 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c582t-a724f9c555e490fa0487526e23872ea0abfc0230fe463f14b4ddffba11c7d6763</citedby><cites>FETCH-LOGICAL-c582t-a724f9c555e490fa0487526e23872ea0abfc0230fe463f14b4ddffba11c7d6763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,1884,27928,27929</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19122415$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsubota, T.(Hokkaido Univ., Sapporo (Japan))</creatorcontrib><creatorcontrib>Sato, M</creatorcontrib><creatorcontrib>Okano, T</creatorcontrib><creatorcontrib>Nakamura, S</creatorcontrib><creatorcontrib>Asano, M</creatorcontrib><creatorcontrib>Komatsu, T</creatorcontrib><creatorcontrib>Shibata, H</creatorcontrib><creatorcontrib>Saito, M</creatorcontrib><title>Annual changes in serum leptin concentration in the adult female Japanese black bear (Ursus thibetanus japonicus)</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>In the present study, assay of the serum leptin concentration of the Japanese black bear (Ursus thibetanus japonicus) was attempted using a canine-leptin-specific sandwich enzyme-linked immunosorbent assay (ELISA). The dose-response curve of the bear serum was linear and parallel to the canine leptin standard curve. In mated and unmated bears, the serum leptin concentration was stable at low levels from May to August or September, gradually increased from September or October, and then remarkably increased in late November. We conclude that this method may be useful for measuring bear serum leptin concentration and that the serum leptin concentration changes annually with a peak in late November.</description><subject>Animals</subject><subject>Animals, Zoo</subject><subject>BLOOD PROTEINS</subject><subject>ELISA</subject><subject>Female</subject><subject>GAIN DE POIDS</subject><subject>GANANCIA DE PESO</subject><subject>Japanese black bear</subject><subject>leptin</subject><subject>Leptin - blood</subject><subject>PROTEINAS SANGUINEAS</subject><subject>PROTEINE SANGUINE</subject><subject>SEASONAL VARIATION</subject><subject>TEST ELISA</subject><subject>Time Factors</subject><subject>URSIDAE</subject><subject>Ursidae - physiology</subject><subject>Ursus thibetanus japonicus</subject><subject>VARIACION ESTACIONAL</subject><subject>VARIATION SAISONNIERE</subject><subject>WEIGHT GAIN</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1r3DAQxUVpabZpb722CAKlgTiVZNlaHcPSrxBID81ZjOXRxq4tO5JcyH9fmV0S6EWjx_x4DO8R8p6zSy60-NL_HeOlyqLU-gXZ8FKqQslSvyQbpnldKFGxE_Imxp4xwWWtX5MTrrkQklcb8nDl_QIDtffg9xhp52nEsIx0wDllYSdv0acAqZv8uk33SKFdhkQdjjAgvYYZPEakzQD2D20QAv18F-ISM9s1mMDnbw_z5Du7xPO35JWDIeK74zwld9--_t79KG5uv__cXd0UttqKVIAS0mlbVRVKzRwwuVWVqFGUWyUQGDTOMlEyh7IuHZeNbFvnGuDcqrZWdXlKPh185zA9LBiTGbtocRjytdMSTa11yaXgGTz7D-ynJfh8m8lxbVVOWVeZujhQNkwxBnRmDt0I4dFwZtYizFqEUVnkIjL-8Wi6NCO2z_Ax-QzsDkAfE-zxCYCQOjvgs5s4vqvt0zbXFQz67PLh4OJgMrAPXTTXvwRjeu265uU_4PKlqw</recordid><startdate>20081201</startdate><enddate>20081201</enddate><creator>Tsubota, T.(Hokkaido Univ., Sapporo (Japan))</creator><creator>Sato, M</creator><creator>Okano, T</creator><creator>Nakamura, S</creator><creator>Asano, M</creator><creator>Komatsu, T</creator><creator>Shibata, H</creator><creator>Saito, M</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20081201</creationdate><title>Annual changes in serum leptin concentration in the adult female Japanese black bear (Ursus thibetanus japonicus)</title><author>Tsubota, T.(Hokkaido Univ., Sapporo (Japan)) ; Sato, M ; Okano, T ; Nakamura, S ; Asano, M ; Komatsu, T ; Shibata, H ; Saito, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c582t-a724f9c555e490fa0487526e23872ea0abfc0230fe463f14b4ddffba11c7d6763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Animals, Zoo</topic><topic>BLOOD PROTEINS</topic><topic>ELISA</topic><topic>Female</topic><topic>GAIN DE POIDS</topic><topic>GANANCIA DE PESO</topic><topic>Japanese black bear</topic><topic>leptin</topic><topic>Leptin - blood</topic><topic>PROTEINAS SANGUINEAS</topic><topic>PROTEINE SANGUINE</topic><topic>SEASONAL VARIATION</topic><topic>TEST ELISA</topic><topic>Time Factors</topic><topic>URSIDAE</topic><topic>Ursidae - physiology</topic><topic>Ursus thibetanus japonicus</topic><topic>VARIACION ESTACIONAL</topic><topic>VARIATION SAISONNIERE</topic><topic>WEIGHT GAIN</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsubota, T.(Hokkaido Univ., Sapporo (Japan))</creatorcontrib><creatorcontrib>Sato, M</creatorcontrib><creatorcontrib>Okano, T</creatorcontrib><creatorcontrib>Nakamura, S</creatorcontrib><creatorcontrib>Asano, M</creatorcontrib><creatorcontrib>Komatsu, T</creatorcontrib><creatorcontrib>Shibata, H</creatorcontrib><creatorcontrib>Saito, M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsubota, T.(Hokkaido Univ., Sapporo (Japan))</au><au>Sato, M</au><au>Okano, T</au><au>Nakamura, S</au><au>Asano, M</au><au>Komatsu, T</au><au>Shibata, H</au><au>Saito, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Annual changes in serum leptin concentration in the adult female Japanese black bear (Ursus thibetanus japonicus)</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2008-12-01</date><risdate>2008</risdate><volume>70</volume><issue>12</issue><spage>1399</spage><epage>1403</epage><pages>1399-1403</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>In the present study, assay of the serum leptin concentration of the Japanese black bear (Ursus thibetanus japonicus) was attempted using a canine-leptin-specific sandwich enzyme-linked immunosorbent assay (ELISA). The dose-response curve of the bear serum was linear and parallel to the canine leptin standard curve. In mated and unmated bears, the serum leptin concentration was stable at low levels from May to August or September, gradually increased from September or October, and then remarkably increased in late November. We conclude that this method may be useful for measuring bear serum leptin concentration and that the serum leptin concentration changes annually with a peak in late November.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>19122415</pmid><doi>10.1292/jvms.70.1399</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Animals, Zoo BLOOD PROTEINS ELISA Female GAIN DE POIDS GANANCIA DE PESO Japanese black bear leptin Leptin - blood PROTEINAS SANGUINEAS PROTEINE SANGUINE SEASONAL VARIATION TEST ELISA Time Factors URSIDAE Ursidae - physiology Ursus thibetanus japonicus VARIACION ESTACIONAL VARIATION SAISONNIERE WEIGHT GAIN |
| title | Annual changes in serum leptin concentration in the adult female Japanese black bear (Ursus thibetanus japonicus) |
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